In [1]:
import Bio
from Bio.Blast.Applications import NcbiblastnCommandline
from Bio import SeqIO
from Bio.Blast import NCBIXML
from Bio import Restriction
from Bio.Restriction import *
from Bio.Alphabet.IUPAC import IUPACAmbiguousDNA
from Bio.Seq import Seq
from Bio.SeqRecord import SeqRecord
from Bio import SeqFeature
import numpy
import cPickle as pickle
import subprocess
import matplotlib as mpl
import matplotlib.pyplot as plt
import matplotlib.font_manager as fm
import matplotlib
import string
import MySQLdb as mdb
import sys
import multiprocessing as mp
from al_funcs import *
from operator import itemgetter, attrgetter, methodcaller
In [2]:
import shelve
In [10]:
# resultssorted = shelve.open("xtch10")
In [11]:
# r[-1]
Out[11]:
SeqRecord(seq=Seq('CAGCCGTAGCCCCATCCCCC', IUPACAmbiguousDNA()), id='227956_R', name='38604220', description='ScrFI', dbxrefs=['Chr10-maingenome'])
In [3]:
guidescoresch10 = pickle.load(open("xt_chromosome10_scored.pkl", "rb"))
resultssorted = sorted(guidescoresch10, reverse=False, key=lambda a: int(attrgetter("name")(a)))
# for item in r:
# resultssorted[] =
# resultssorted.sync()
In [4]:
del r
del guidescoresch10
---------------------------------------------------------------------------
NameError Traceback (most recent call last)
<ipython-input-4-fafb3854f24d> in <module>()
----> 1 del r
2 del guidescoresch10
NameError: name 'r' is not defined
In [5]:
# Set variables:
#genomefasta = "../../Genomic Data/LAEVIS_7.1.repeatMasked.fa" #"xl71"
genomefasta = "../../Genomic Data/xt80plus.fasta" # "xt80plus"
genomename = "xt80plus"
In [6]:
# This makes a dict of the genome for score lookups
genome = SeqIO.parse(open(genomefasta, 'rb'), "fasta", alphabet=IUPACAmbiguousDNA())
genomedict = {}
for item in genome:
genomedict[item.id] = item
In [7]:
chromosome = "Chr10-maingenome"
In [8]:
class amplicon:
'''
A complete amplicon for use in EATING; the class contains methods for designing
amplifying primers and describing the guides within it.
properties:
.start, .end: count of runs along chromosome
.fiveprimeabut, .threeprimeabut: count of guides along chromosome that abut good guides
.guides: list of good guides in run
.length: permissible region distance
.guidecount: number of guides in list (=len(self.guides))
.genomename: genomename
.chromosome
.left_outside: the id of the guide to the left of the outside
.left_inside: ...
.permissible_start: absolute numbering vs chromosome
.required_start_absolute: absolute numbering vs chromosome
.required_start_relative
.permissible_region
'''
def __init__(self, run):
self.start = run[0]
self.end = run[1]
try:
self.fiveprimeabut = scores_and_details[self.start-1]
self.threeprimeabut= scores_and_details[self.end]
except:
self.fiveprimeabut = "0"
self.threeprimeabut = "None"
self.guides = scores_and_details[self.start:self.end]
self.length = int(self.threeprimeabut.name)-int(self.fiveprimeabut.name)
self.guidecount = self.end - self.start
self.genomename = genomename
self.chromosome = chromosome
# Define the sequences necessary
def pullsequences(self):
self.left_outside = self.fiveprimeabut.id[-1]
self.left_inside = self.guides[0].id[-1]
if self.left_outside == "F" and self.left_inside == "R":
self.permissible_start = int(self.fiveprimeabut.name) + 10
self.required_start_absolute = int(self.guides[0].name) +14
elif self.left_outside == "R" and self.left_inside == "R":
self.permissible_start = int(self.fiveprimeabut.name) + 1
self.required_start_absolute = int(self.guides[0].name) +14
elif self.left_outside == "R" and self.left_inside == "F":
self.permissible_start = int(self.fiveprimeabut.name) + 1
self.required_start_absolute = int(self.guides[0].name) +18
elif self.left_outside == "F" and self.left_inside == "F":
self.permissible_start = int(self.fiveprimeabut.name) + 10
self.required_start_absolute = int(self.guides[0].name) +18
else:
print("error on left")
# (fiveprimeabuttingguide, threeprimeabuttingguide), end-start, scores_and_details[start:end]))
#self.right_inside = item[2][-1][1].id[-1]
self.right_inside = self.guides[-1].id[-1]
self.right_outside = self.threeprimeabut.id[-1]
if self.right_outside == "F" and self.right_inside == "R":
self.permissible_end = int(self.threeprimeabut.name) + 19
self.required_end_absolute = int(self.guides[-1].name) + 2
elif self.right_outside == "R" and self.right_inside == "F":
self.permissible_end = int(self.threeprimeabut.name) + 10
self.required_end_absolute = int(self.guides[-1].name) + 8
elif self.right_outside == "R" and self.right_inside == "R":
self.permissible_end = int(self.threeprimeabut.name) + 10
self.required_end_absolute = int(self.guides[-1].name) + 2
elif self.right_outside == "F" and self.right_inside == "F":
self.permissible_end = int(self.threeprimeabut.name) + 19
self.required_end_absolute = int(self.guides[-1].name) + 8
else:
print("error on right")
self.permissible_region = annotchrom[self.permissible_start:self.permissible_end]
# Bounds that need to be included in PCR product :
self.required_start_relative = self.required_start_absolute-self.permissible_start
self.required_end_relative = self.required_end_absolute - self.permissible_start
#self.amp.dbxrefs=((self.required_start_relative, self.required_end_relative))
# Set up some other stuff:
self.permissible_region.name =str(self.fiveprimeabut.name)
self.permissible_region.id =str(self.fiveprimeabut.name)
self.permissible_region.description=str(self.guidecount)
self.permissible_region.seq.alphabet = IUPACAmbiguousDNA()
In [9]:
#Original version using guide list
probeyield = []
x = 94 #Threshold
amplicon_positions = []
scores = [(details.annotations["score"], len(details)) for details in resultssorted]
scores_and_details = resultssorted
runs=[]
ends=[]
previtemgood = 0
for index, (item, guide_length) in enumerate(scores):
if item >= x and previtemgood ==0 and guide_length > 19:
runs.append(index)
previtemgood = 1
elif item >= x and previtemgood == 1 and guide_length > 19:
None
elif previtemgood == 1:
previtemgood = 0
ends.append(index)
runs = zip(runs, ends)
for i in runs:
if i[1] - i[0] > 3:
amplicon_positions.append(amplicon(i))
goodruns = sorted(amplicon_positions, reverse=True, key=attrgetter("guidecount"))# sorts by the number of guides in list
In [10]:
for item in goodruns:
item.pullsequences()
---------------------------------------------------------------------------
NameError Traceback (most recent call last)
<ipython-input-10-9164ec469044> in <module>()
1 for item in goodruns:
----> 2 item.pullsequences()
<ipython-input-8-d92d5f7112a3> in pullsequences(self)
71 print("error on right")
72
---> 73 self.permissible_region = annotchrom[self.permissible_start:self.permissible_end]
74 # Bounds that need to be included in PCR product :
75 self.required_start_relative = self.required_start_absolute-self.permissible_start
NameError: global name 'annotchrom' is not defined
In [213]:
# This double-checks the score of the guides in the "goodruns" due to some weirdness with a portion of the scores in the
# SQL database. If they're bad, it adds them to badruns.
badruns = []
for index, k in enumerate(goodruns[0:1000]):
if index > 499: #only necessary if resuming the calculation part way through the list
for item in k.guides:
rescore = al_scoreguide(item, "xt80plus", genomedict)
if rescore.annotations["score"] < 94:
print(index, item, rescore.annotations["score"])
badruns.append(index)
(507, SeqRecord(seq=Seq('ATTGTGACTTTCCTTGGGCC', IUPACAmbiguousDNA()), id='166904_R', name='13841357', description='ScrFI', dbxrefs=['Chr10-maingenome']), 0)
(526, SeqRecord(seq=Seq('AGAACTATCAGGGCCAGAAC', IUPACAmbiguousDNA()), id='91255_F', name='21968004', description='BfaI', dbxrefs=['Chr10-maingenome']), 91)
(526, SeqRecord(seq=Seq('ATGCATCTTCTGCCTACACC', IUPACAmbiguousDNA()), id='91256_R', name='21968026', description='BfaI', dbxrefs=['Chr10-maingenome']), 16)
(526, SeqRecord(seq=Seq('CTTAGGGCAGTGATCCCAAC', IUPACAmbiguousDNA()), id='91259_F', name='21968715', description='BfaI', dbxrefs=['Chr10-maingenome']), 25)
(526, SeqRecord(seq=Seq('ACATGGTGCTGGTGAGCCAC', IUPACAmbiguousDNA()), id='91260_R', name='21968737', description='BfaI', dbxrefs=['Chr10-maingenome']), 28)
(528, SeqRecord(seq=Seq('CGCAGGTTAAGTACCATACC', IUPACAmbiguousDNA()), id='186803_F', name='22602870', description='ScrFI', dbxrefs=['Chr10-maingenome']), 48)
(528, SeqRecord(seq=Seq('TAGTGTATTTAACCTATACC', IUPACAmbiguousDNA()), id='186804_R', name='22602891', description='ScrFI', dbxrefs=['Chr10-maingenome']), 25)
(528, SeqRecord(seq=Seq('TAAAAGTTAAGCACAGTGCC', IUPACAmbiguousDNA()), id='186804_F', name='22603028', description='ScrFI', dbxrefs=['Chr10-maingenome']), 66)
(528, SeqRecord(seq=Seq('CACTGTACTTAACCTGTGCC', IUPACAmbiguousDNA()), id='186805_R', name='22603049', description='ScrFI', dbxrefs=['Chr10-maingenome']), 4)
(528, SeqRecord(seq=Seq('CACAGGTTAAGTACAGTGCC', IUPACAmbiguousDNA()), id='186805_F', name='22603051', description='ScrFI', dbxrefs=['Chr10-maingenome']), 1)
(528, SeqRecord(seq=Seq('CACTGTACTTAACCCGTACC', IUPACAmbiguousDNA()), id='186806_R', name='22603072', description='ScrFI', dbxrefs=['Chr10-maingenome']), 12)
(528, SeqRecord(seq=Seq('TACGGGTTAAGTACAGTGCC', IUPACAmbiguousDNA()), id='186806_F', name='22603074', description='ScrFI', dbxrefs=['Chr10-maingenome']), 8)
(528, SeqRecord(seq=Seq('CACTGTACTTAACCCGTACC', IUPACAmbiguousDNA()), id='186807_R', name='22603095', description='ScrFI', dbxrefs=['Chr10-maingenome']), 12)
(528, SeqRecord(seq=Seq('TACGGGTTAAGTACAGTGCC', IUPACAmbiguousDNA()), id='186807_F', name='22603097', description='ScrFI', dbxrefs=['Chr10-maingenome']), 8)
(528, SeqRecord(seq=Seq('CACTGTACTTAACCTGTACC', IUPACAmbiguousDNA()), id='186808_R', name='22603118', description='ScrFI', dbxrefs=['Chr10-maingenome']), 6)
(528, SeqRecord(seq=Seq('TACAGGTTAAGTACAGTGCC', IUPACAmbiguousDNA()), id='186808_F', name='22603120', description='ScrFI', dbxrefs=['Chr10-maingenome']), 1)
(528, SeqRecord(seq=Seq('CACTGTACTTAACCTGTACC', IUPACAmbiguousDNA()), id='186809_R', name='22603141', description='ScrFI', dbxrefs=['Chr10-maingenome']), 6)
(528, SeqRecord(seq=Seq('TACAGGTTAAGTACAGTGCC', IUPACAmbiguousDNA()), id='186809_F', name='22603143', description='ScrFI', dbxrefs=['Chr10-maingenome']), 1)
(528, SeqRecord(seq=Seq('GAACAGGGGGAGAGAGAGAC', IUPACAmbiguousDNA()), id='24281_F', name='22603658', description='HpaII', dbxrefs=['Chr10-maingenome']), 14)
(528, SeqRecord(seq=Seq('AACAGGGGGAGAGAGAGACC', IUPACAmbiguousDNA()), id='186811_F', name='22603659', description='ScrFI', dbxrefs=['Chr10-maingenome']), 24)
(528, SeqRecord(seq=Seq('CCTGCCTTACATAGTCCTCC', IUPACAmbiguousDNA()), id='24282_R', name='22603680', description='HpaII', dbxrefs=['Chr10-maingenome']), 11)
(528, SeqRecord(seq=Seq('CCTGCCTTACATAGTCCTCC', IUPACAmbiguousDNA()), id='186812_R', name='22603680', description='ScrFI', dbxrefs=['Chr10-maingenome']), 11)
(528, SeqRecord(seq=Seq('GGCAAAGGCAGAGGCTGACC', IUPACAmbiguousDNA()), id='186812_F', name='22603724', description='ScrFI', dbxrefs=['Chr10-maingenome']), 13)
(539, SeqRecord(seq=Seq('ACTTTTTTCTTTCCCCCACC', IUPACAmbiguousDNA()), id='203386_R', name='29685279', description='ScrFI', dbxrefs=['Chr10-maingenome']), 85)
(539, SeqRecord(seq=Seq('AATCCAATACAGTGACCCCC', IUPACAmbiguousDNA()), id='203390_R', name='29686012', description='ScrFI', dbxrefs=['Chr10-maingenome']), 52)
(559, SeqRecord(seq=Seq('TATTGAGAAAATGTAGGCCC', IUPACAmbiguousDNA()), id='134471_R', name='2158209', description='ScrFI', dbxrefs=['Chr10-maingenome']), 62)
(567, SeqRecord(seq=Seq('ATAATGCCCCTTTAATTACC', IUPACAmbiguousDNA()), id='141781_F', name='4351776', description='ScrFI', dbxrefs=['Chr10-maingenome']), 83)
(571, SeqRecord(seq=Seq('AAAGGCAATACTACTTGGCC', IUPACAmbiguousDNA()), id='7511_R', name='4884460', description='HpaII', dbxrefs=['Chr10-maingenome']), 4)
(571, SeqRecord(seq=Seq('AAAGGCAATACTACTTGGCC', IUPACAmbiguousDNA()), id='143296_R', name='4884460', description='ScrFI', dbxrefs=['Chr10-maingenome']), 4)
(600, SeqRecord(seq=Seq('GTGGCCAACTCAGTGGTTCC', IUPACAmbiguousDNA()), id='170787_R', name='15480364', description='ScrFI', dbxrefs=['Chr10-maingenome']), 84)
(602, SeqRecord(seq=Seq('TAGAACTATAAAATCTGCAC', IUPACAmbiguousDNA()), id='20742_F', name='18113850', description='HpaII', dbxrefs=['Chr10-maingenome']), 93)
(603, SeqRecord(seq=Seq('GAGATGCTGAAACTTTTGGC', IUPACAmbiguousDNA()), id='85675_R', name='19547227', description='BfaI', dbxrefs=['Chr10-maingenome']), 1)
(611, SeqRecord(seq=Seq('AGAGAAGTCTATATTGTTTC', IUPACAmbiguousDNA()), id='97880_R', name='24748430', description='BfaI', dbxrefs=['Chr10-maingenome']), 62)
(611, SeqRecord(seq=Seq('ATATACCTGAATAAGTATAC', IUPACAmbiguousDNA()), id='97886_F', name='24751600', description='BfaI', dbxrefs=['Chr10-maingenome']), 78)
(611, SeqRecord(seq=Seq('ACAACAGATTCATTCATCAC', IUPACAmbiguousDNA()), id='97888_R', name='24751833', description='BfaI', dbxrefs=['Chr10-maingenome']), 24)
(624, SeqRecord(seq=Seq('TCTCTCACCCCTTCCCATTC', IUPACAmbiguousDNA()), id='106552_F', name='28499110', description='BfaI', dbxrefs=['Chr10-maingenome']), 66)
(624, SeqRecord(seq=Seq('CAAAAAGTGGATGAACATAC', IUPACAmbiguousDNA()), id='106553_R', name='28499132', description='BfaI', dbxrefs=['Chr10-maingenome']), 66)
(624, SeqRecord(seq=Seq('CCCTCCTAAACCTGCTGCCC', IUPACAmbiguousDNA()), id='106555_R', name='28500270', description='BfaI', dbxrefs=['Chr10-maingenome']), 25)
(624, SeqRecord(seq=Seq('TGTGGCCTCTCCACAAATCC', IUPACAmbiguousDNA()), id='200362_R', name='28500546', description='ScrFI', dbxrefs=['Chr10-maingenome']), 0)
(645, SeqRecord(seq=Seq('GTCCCAGCATGCCATTGTGC', IUPACAmbiguousDNA()), id='1928_F', name='933192', description='HpaII', dbxrefs=['Chr10-maingenome']), 48)
(658, SeqRecord(seq=Seq('TAAAACCATGAGATCAATAC', IUPACAmbiguousDNA()), id='48515_R', name='3075581', description='BfaI', dbxrefs=['Chr10-maingenome']), 79)
(658, SeqRecord(seq=Seq('CCAGGGGAGTTACTGGATTC', IUPACAmbiguousDNA()), id='48516_R', name='3075814', description='BfaI', dbxrefs=['Chr10-maingenome']), 63)
(658, SeqRecord(seq=Seq('AAAAAAACTGGGACAAAAAC', IUPACAmbiguousDNA()), id='48517_R', name='3076766', description='BfaI', dbxrefs=['Chr10-maingenome']), 83)
(676, SeqRecord(seq=Seq('GGAGATGGTGTGGCACAGCC', IUPACAmbiguousDNA()), id='145611_R', name='5671887', description='ScrFI', dbxrefs=['Chr10-maingenome']), 62)
(697, SeqRecord(seq=Seq('GGGAATTATTTGGGCTGGAC', IUPACAmbiguousDNA()), id='68072_R', name='11728227', description='BfaI', dbxrefs=['Chr10-maingenome']), 90)
(703, SeqRecord(seq=Seq('AATGCTCAAATATCACATAC', IUPACAmbiguousDNA()), id='17236_F', name='14026077', description='HpaII', dbxrefs=['Chr10-maingenome']), 5)
(710, SeqRecord(seq=Seq('ATTGTGACTTTCCTTGGGCC', IUPACAmbiguousDNA()), id='171645_F', name='15861938', description='ScrFI', dbxrefs=['Chr10-maingenome']), 0)
(725, SeqRecord(seq=Seq('CCAACTCTTCCAGTAGCACC', IUPACAmbiguousDNA()), id='194214_F', name='25739167', description='ScrFI', dbxrefs=['Chr10-maingenome']), 87)
(725, SeqRecord(seq=Seq('CAGGGTGCAACTGGTTTCCC', IUPACAmbiguousDNA()), id='194215_R', name='25739188', description='ScrFI', dbxrefs=['Chr10-maingenome']), 62)
(725, SeqRecord(seq=Seq('AAGGGTGATGCTGGTCCTCC', IUPACAmbiguousDNA()), id='194218_R', name='25739598', description='ScrFI', dbxrefs=['Chr10-maingenome']), 85)
(726, SeqRecord(seq=Seq('CTGTGCCCAAATATACATGC', IUPACAmbiguousDNA()), id='101155_R', name='26089433', description='BfaI', dbxrefs=['Chr10-maingenome']), 90)
(726, SeqRecord(seq=Seq('TCTCATTTCAAACCACTGCC', IUPACAmbiguousDNA()), id='101157_F', name='26090346', description='BfaI', dbxrefs=['Chr10-maingenome']), 13)
(726, SeqRecord(seq=Seq('GTTCAAATTGCCATAGCAAC', IUPACAmbiguousDNA()), id='101158_R', name='26090368', description='BfaI', dbxrefs=['Chr10-maingenome']), 3)
(726, SeqRecord(seq=Seq('TGCTATGGCAATTTGAACCC', IUPACAmbiguousDNA()), id='101158_F', name='26090370', description='BfaI', dbxrefs=['Chr10-maingenome']), 3)
(726, SeqRecord(seq=Seq('TTGAGCAAGTATCTGGTTGC', IUPACAmbiguousDNA()), id='101159_R', name='26090392', description='BfaI', dbxrefs=['Chr10-maingenome']), 20)
(783, SeqRecord(seq=Seq('TACTTTCTAATATTTTTTTC', IUPACAmbiguousDNA()), id='54869_R', name='5904094', description='BfaI', dbxrefs=['Chr10-maingenome']), 54)
(783, SeqRecord(seq=Seq('GATTTTTAAAAAGCGATTAC', IUPACAmbiguousDNA()), id='54873_F', name='5907748', description='BfaI', dbxrefs=['Chr10-maingenome']), 84)
(790, SeqRecord(seq=Seq('GATGGGCGAAATGTTTCGCC', IUPACAmbiguousDNA()), id='152296_R', name='7771930', description='ScrFI', dbxrefs=['Chr10-maingenome']), 0)
(792, SeqRecord(seq=Seq('CTGCTTTATGGCTGACATTC', IUPACAmbiguousDNA()), id='60012_R', name='8173656', description='BfaI', dbxrefs=['Chr10-maingenome']), 0)
(805, SeqRecord(seq=Seq('GTACAAAGAACAAAGAAACC', IUPACAmbiguousDNA()), id='157530_F', name='9815673', description='ScrFI', dbxrefs=['Chr10-maingenome']), 93)
(816, SeqRecord(seq=Seq('GAGCAGGATGATGATAATAC', IUPACAmbiguousDNA()), id='69196_F', name='12239761', description='BfaI', dbxrefs=['Chr10-maingenome']), 48)
(816, SeqRecord(seq=Seq('CTTGTCCTACTGTCTTGATC', IUPACAmbiguousDNA()), id='69197_R', name='12239783', description='BfaI', dbxrefs=['Chr10-maingenome']), 50)
(816, SeqRecord(seq=Seq('GAGCAGGATGATGATAATAC', IUPACAmbiguousDNA()), id='69197_F', name='12239967', description='BfaI', dbxrefs=['Chr10-maingenome']), 48)
(816, SeqRecord(seq=Seq('CTTGTCCTACTGTCTTGATC', IUPACAmbiguousDNA()), id='69198_R', name='12239989', description='BfaI', dbxrefs=['Chr10-maingenome']), 50)
(816, SeqRecord(seq=Seq('GAGCAGGATGATGATAATAC', IUPACAmbiguousDNA()), id='69198_F', name='12240097', description='BfaI', dbxrefs=['Chr10-maingenome']), 48)
(816, SeqRecord(seq=Seq('CTTGTCCTACTGTCTTGATC', IUPACAmbiguousDNA()), id='69199_R', name='12240119', description='BfaI', dbxrefs=['Chr10-maingenome']), 50)
(816, SeqRecord(seq=Seq('TAAACACTTATATGCTGTTC', IUPACAmbiguousDNA()), id='69200_R', name='12241152', description='BfaI', dbxrefs=['Chr10-maingenome']), 66)
(816, SeqRecord(seq=Seq('TCTAACCCTAATTGACCTTC', IUPACAmbiguousDNA()), id='69200_F', name='12241179', description='BfaI', dbxrefs=['Chr10-maingenome']), 14)
(823, SeqRecord(seq=Seq('TTTCAATAGTCAGAACCACC', IUPACAmbiguousDNA()), id='170710_R', name='15445371', description='ScrFI', dbxrefs=['Chr10-maingenome']), 4)
(844, SeqRecord(seq=Seq('TATTTATTTGCAGTTTTCTC', IUPACAmbiguousDNA()), id='97848_R', name='24736905', description='BfaI', dbxrefs=['Chr10-maingenome']), 89)
(844, SeqRecord(seq=Seq('CAGTACCCAAGGGTGTATTC', IUPACAmbiguousDNA()), id='97851_R', name='24737495', description='BfaI', dbxrefs=['Chr10-maingenome']), 30)
(844, SeqRecord(seq=Seq('TTTTTACTTTGTGCTCCACC', IUPACAmbiguousDNA()), id='97853_R', name='24738319', description='BfaI', dbxrefs=['Chr10-maingenome']), 49)
(844, SeqRecord(seq=Seq('AAATGCTGATAAAAAAAATC', IUPACAmbiguousDNA()), id='97853_F', name='24738434', description='BfaI', dbxrefs=['Chr10-maingenome']), 91)
(844, SeqRecord(seq=Seq('CTGTCTGAAAACACAGCAGC', IUPACAmbiguousDNA()), id='97854_R', name='24738456', description='BfaI', dbxrefs=['Chr10-maingenome']), 63)
(854, SeqRecord(seq=Seq('CCACTTTCTAAACTAAGGTC', IUPACAmbiguousDNA()), id='104177_R', name='27506368', description='BfaI', dbxrefs=['Chr10-maingenome']), 87)
(854, SeqRecord(seq=Seq('CCTTTTATTACGTTCCCCCC', IUPACAmbiguousDNA()), id='104178_R', name='27506668', description='BfaI', dbxrefs=['Chr10-maingenome']), 88)
(866, SeqRecord(seq=Seq('GCACCGAACGATCGCACGTC', IUPACAmbiguousDNA()), id='111434_F', name='30506967', description='BfaI', dbxrefs=['Chr10-maingenome']), 47)
(866, SeqRecord(seq=Seq('AATGGCGGATGAATAATCGC', IUPACAmbiguousDNA()), id='111435_R', name='30506989', description='BfaI', dbxrefs=['Chr10-maingenome']), 65)
(868, SeqRecord(seq=Seq('TTCCTATGCTTCAAAATGGC', IUPACAmbiguousDNA()), id='111478_F', name='30541237', description='BfaI', dbxrefs=['Chr10-maingenome']), 72)
(882, SeqRecord(seq=Seq('TATCTACCTATCTATCTACC', IUPACAmbiguousDNA()), id='118698_R', name='33939212', description='BfaI', dbxrefs=['Chr10-maingenome']), 14)
(884, SeqRecord(seq=Seq('TTAGTCCCTCCTCTCCTGCC', IUPACAmbiguousDNA()), id='216157_R', name='34445402', description='ScrFI', dbxrefs=['Chr10-maingenome']), 0)
(885, SeqRecord(seq=Seq('ATGGCACCTTACAGCCCCCC', IUPACAmbiguousDNA()), id='216444_R', name='34550793', description='ScrFI', dbxrefs=['Chr10-maingenome']), 0)
(891, SeqRecord(seq=Seq('AGAGGAGAGAGTTCTGCACC', IUPACAmbiguousDNA()), id='224578_R', name='37398525', description='ScrFI', dbxrefs=['Chr10-maingenome']), 93)
(891, SeqRecord(seq=Seq('GAGTTAAGGTGGCCATACCC', IUPACAmbiguousDNA()), id='224582_F', name='37399154', description='ScrFI', dbxrefs=['Chr10-maingenome']), 11)
(891, SeqRecord(seq=Seq('CTCACCAGCTAAAATCTGCC', IUPACAmbiguousDNA()), id='224583_R', name='37399175', description='ScrFI', dbxrefs=['Chr10-maingenome']), 31)
(928, SeqRecord(seq=Seq('CCACCTTGTGTTACTGCCCC', IUPACAmbiguousDNA()), id='4837_R', name='2882424', description='HpaII', dbxrefs=['Chr10-maingenome']), 50)
(928, SeqRecord(seq=Seq('CCACCTTGTGTTACTGCCCC', IUPACAmbiguousDNA()), id='137125_R', name='2882424', description='ScrFI', dbxrefs=['Chr10-maingenome']), 50)
(942, SeqRecord(seq=Seq('CCACCCAAAAGCTACTGCCC', IUPACAmbiguousDNA()), id='54892_F', name='5916018', description='BfaI', dbxrefs=['Chr10-maingenome']), 57)
(953, SeqRecord(seq=Seq('TGCCAGGGGTTGCAAGGTGC', IUPACAmbiguousDNA()), id='60181_R', name='8240090', description='BfaI', dbxrefs=['Chr10-maingenome']), 35)
(953, SeqRecord(seq=Seq('CCACTGTTCCTTTAAAGAGC', IUPACAmbiguousDNA()), id='60185_R', name='8242634', description='BfaI', dbxrefs=['Chr10-maingenome']), 65)
(962, SeqRecord(seq=Seq('TCTGTAACCTTGTTATGAGC', IUPACAmbiguousDNA()), id='64782_F', name='10350653', description='BfaI', dbxrefs=['Chr10-maingenome']), 6)
(970, SeqRecord(seq=Seq('GTTATAGAAAACCCTATTCC', IUPACAmbiguousDNA()), id='68084_F', name='11734623', description='BfaI', dbxrefs=['Chr10-maingenome']), 3)
(991, SeqRecord(seq=Seq('GCTGTGTGAGCCTCACAGCC', IUPACAmbiguousDNA()), id='179474_R', name='19436504', description='ScrFI', dbxrefs=['Chr10-maingenome']), 66)
In [229]:
# goodruns_filtered= [] #uncomment if it's a new goodruns_filtered list, rather than a pkl loaded one.
for index, item in enumerate(goodruns[:1000]):
if index > 499:
if index not in set(badruns):
goodruns_filtered.append(item)
In [230]:
len(set(goodruns_filtered))
Out[230]:
898
In [231]:
sum([item.guidecount for item in goodruns_filtered[:432]])
Out[231]:
13730
So, after filtering out the improperly scored guides, the top 432 amplicons contain 13730 guides.
In [340]:
# This tries to describe whether the runs are sort of evenly spaced along the chromosome
%pylab inline
plt.plot(sorted([int(item.guides[1].name) for item in exp50[0:432]]))
Populating the interactive namespace from numpy and matplotlib
WARNING: pylab import has clobbered these variables: ['random']
`%matplotlib` prevents importing * from pylab and numpy
Out[340]:
[<matplotlib.lines.Line2D at 0x1fe461810>]
In [1]:
relativeserver = "localhost"
In [71]:
def scoredblookup(guide, genome):
con = mdb.connect(relativeserver, 'root', '2399lane', 'guidescores');
cur = con.cursor(mdb.cursors.DictCursor)
cur.execute("SELECT score, sequence, version FROM scores WHERE sequence='{0}' AND genome = '{1}'"\
.format(str(guide.seq), genome))
dbresult = cur.fetchall()
#guide.annotations["score"] = dbresult[0]["score"]
return dbresult
In [71]:
for item in resultssorted:
t = scoredblookup(item, genomename)
if len(t) ==2 and t[0]["score"] != t[1]["score"]:
print str(t) + "\n"
({'score': 99, 'version': 0L, 'sequence': 'CTGCTTTATGGCTGACATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CTGCTTTATGGCTGACATTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATGTACCTCTTCTGCACCCC'}, {'score': 16, 'version': 2L, 'sequence': 'ATGTACCTCTTCTGCACCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGACAGATTCGCTCATTAC'}, {'score': 0, 'version': 2L, 'sequence': 'GGGACAGATTCGCTCATTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATGTACCTCTTCTGCACCCC'}, {'score': 16, 'version': 2L, 'sequence': 'ATGTACCTCTTCTGCACCCC'})
({'score': 1, 'version': 0L, 'sequence': 'ATAGATAGATAGATAGATGC'}, {'score': 0, 'version': 2L, 'sequence': 'ATAGATAGATAGATAGATGC'})
({'score': 1, 'version': 0L, 'sequence': 'ATAGATAGATAGATAGATGC'}, {'score': 0, 'version': 2L, 'sequence': 'ATAGATAGATAGATAGATGC'})
({'score': 99, 'version': 0L, 'sequence': 'TTAAACCACTGTCTGGTTGC'}, {'score': 0, 'version': 2L, 'sequence': 'TTAAACCACTGTCTGGTTGC'})
({'score': 99, 'version': 0L, 'sequence': 'TTAAACCACTGTCTGGTTGC'}, {'score': 0, 'version': 2L, 'sequence': 'TTAAACCACTGTCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTAAGGTAAACAAAACCC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCTAAGGTAAACAAAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGGTCCAGTTTAACC'}, {'score': 99, 'version': 2L, 'sequence': 'TAGGGTCCAGTTTAACC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 87, 'version': 0L, 'sequence': 'TAGAACCCTACAGCTGCC'}, {'score': 91, 'version': 2L, 'sequence': 'TAGAACCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 87, 'version': 0L, 'sequence': 'TAGAACCCTACAGCTGCC'}, {'score': 91, 'version': 2L, 'sequence': 'TAGAACCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 94, 'version': 0L, 'sequence': 'TAGAGACCTACAGCTGCC'}, {'score': 96, 'version': 2L, 'sequence': 'TAGAGACCTACAGCTGCC'})
({'score': 94, 'version': 0L, 'sequence': 'TAGAGACCTACAGCTGCC'}, {'score': 96, 'version': 2L, 'sequence': 'TAGAGACCTACAGCTGCC'})
({'score': 87, 'version': 0L, 'sequence': 'TAGAACCCTACAGCTGCC'}, {'score': 91, 'version': 2L, 'sequence': 'TAGAACCCTACAGCTGCC'})
({'score': 89, 'version': 0L, 'sequence': 'TAGAGCCCAACAGCTGCC'}, {'score': 91, 'version': 2L, 'sequence': 'TAGAGCCCAACAGCTGCC'})
({'score': 99, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGTC'}, {'score': 98, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGTC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 87, 'version': 0L, 'sequence': 'TAGAACCCTACAGCTGCC'}, {'score': 91, 'version': 2L, 'sequence': 'TAGAACCCTACAGCTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTAAGCAGCTGTAGGGCTC'}, {'score': 5, 'version': 2L, 'sequence': 'TCTAAGCAGCTGTAGGGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTAGAGCCCTACAGCTGCC'}, {'score': 49, 'version': 2L, 'sequence': 'CTTAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 87, 'version': 0L, 'sequence': 'TAGAACCCTACAGCTGCC'}, {'score': 91, 'version': 2L, 'sequence': 'TAGAACCCTACAGCTGCC'})
({'score': 89, 'version': 0L, 'sequence': 'TAGAGCCCAACAGCTGCC'}, {'score': 91, 'version': 2L, 'sequence': 'TAGAGCCCAACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 98, 'version': 0L, 'sequence': 'TCTGGGCAGCTGTAGGGCTC'}, {'score': 66, 'version': 2L, 'sequence': 'TCTGGGCAGCTGTAGGGCTC'})
({'score': 94, 'version': 0L, 'sequence': 'CCCAGAACCCTACAGCTGCC'}, {'score': 55, 'version': 2L, 'sequence': 'CCCAGAACCCTACAGCTGCC'})
({'score': 89, 'version': 0L, 'sequence': 'TAGAGCCCAACAGCTGCC'}, {'score': 91, 'version': 2L, 'sequence': 'TAGAGCCCAACAGCTGCC'})
({'score': 89, 'version': 0L, 'sequence': 'TAGAGCCCAACAGCTGCC'}, {'score': 91, 'version': 2L, 'sequence': 'TAGAGCCCAACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 84, 'version': 0L, 'sequence': 'TAGAGCCCTACAGCTGCC'}, {'score': 90, 'version': 2L, 'sequence': 'TAGAGCCCTACAGCTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATACCGGCCAATCCC'}, {'score': 6, 'version': 2L, 'sequence': 'GTCGGATACCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGGTATCCGACCGC'}, {'score': 9, 'version': 2L, 'sequence': 'ATTGGCCGGTATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCAC'}, {'score': 20, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGGTATCTGACCGC'}, {'score': 69, 'version': 2L, 'sequence': 'ATTGGCCGGTATCTGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTCGGATATTGGCCATCCC'}, {'score': 40, 'version': 2L, 'sequence': 'GGTCGGATATTGGCCATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GATGGCCAATATCCGACCGC'}, {'score': 86, 'version': 2L, 'sequence': 'GATGGCCAATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCAGATATTGGCCAATACC'}, {'score': 99, 'version': 2L, 'sequence': 'GTCAGATATTGGCCAATACC'})
({'score': 100, 'version': 0L, 'sequence': 'CAATATCTGACCGCTAAATC'}, {'score': 66, 'version': 2L, 'sequence': 'CAATATCTGACCGCTAAATC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCAGATATTGGCCAATCCC'}, {'score': 99, 'version': 2L, 'sequence': 'GTCAGATATTGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CAATATCTGACCGCTAAATC'}, {'score': 66, 'version': 2L, 'sequence': 'CAATATCTGACCGCTAAATC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCAC'}, {'score': 20, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GACGGATACCGGCCAATCCC'}, {'score': 86, 'version': 2L, 'sequence': 'GACGGATACCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATACCGGCCAATCCC'}, {'score': 6, 'version': 2L, 'sequence': 'GTCGGATACCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGGTATCCGACCGC'}, {'score': 9, 'version': 2L, 'sequence': 'ATTGGCCGGTATCCGACCGC'})
({'score': 99, 'version': 0L, 'sequence': 'GGTCAGATATTGGCCATCCC'}, {'score': 33, 'version': 2L, 'sequence': 'GGTCAGATATTGGCCATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GATGGCCAATATCTGACCGC'}, {'score': 87, 'version': 2L, 'sequence': 'GATGGCCAATATCTGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCAC'}, {'score': 17, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TATCGGCCAATCCTTAGATC'}, {'score': 19, 'version': 2L, 'sequence': 'TATCGGCCAATCCTTAGATC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACTGC'}, {'score': 22, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCAC'}, {'score': 20, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCAC'}, {'score': 20, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TATCGGCCAATCCTTAGATC'}, {'score': 19, 'version': 2L, 'sequence': 'TATCGGCCAATCCTTAGATC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACTGC'}, {'score': 22, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACTGC'}, {'score': 22, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCAC'}, {'score': 20, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATACCGGCCAATCCC'}, {'score': 6, 'version': 2L, 'sequence': 'GTCGGATACCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGGTATCCGACCGC'}, {'score': 9, 'version': 2L, 'sequence': 'ATTGGCCGGTATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTCGGATATTGGCCATCCC'}, {'score': 40, 'version': 2L, 'sequence': 'GGTCGGATATTGGCCATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GATGGCCAATATCCGACCAC'}, {'score': 99, 'version': 2L, 'sequence': 'GATGGCCAATATCCGACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TATCGGCCAATCCTTAGATC'}, {'score': 19, 'version': 2L, 'sequence': 'TATCGGCCAATCCTTAGATC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACTGC'}, {'score': 22, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCAC'}, {'score': 20, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACTGC'}, {'score': 22, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCAC'}, {'score': 17, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCAGCCAATCCC'}, {'score': 5, 'version': 2L, 'sequence': 'GTCGGATATCAGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCTGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCTGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACTGC'}, {'score': 22, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACTGC'}, {'score': 22, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACTGC'}, {'score': 22, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCGATATCCGACCGC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTGGCCGATATCCGACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGGATATCGGCCAATCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTCGGATATCGGCCAATCCC'})
({'score': 47, 'version': 0L, 'sequence': 'CGATATCCGACCGCTAAATC'}, {'score': 100, 'version': 2L, 'sequence': 'CGATATCCGACCGCTAAATC'})
({'score': 8, 'version': 0L, 'sequence': 'GTCGGATATCGACCAATCCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTCGGATATCGACCAATCCC'})
({'score': 47, 'version': 0L, 'sequence': 'CGATATCCGACCGCTAAATC'}, {'score': 100, 'version': 2L, 'sequence': 'CGATATCCGACCGCTAAATC'})
({'score': 97, 'version': 0L, 'sequence': 'GTTGGATATCGACCAATCCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTGGATATCGACCAATCCC'})
({'score': 45, 'version': 0L, 'sequence': 'CGATATCCAACCGCTAAATC'}, {'score': 100, 'version': 2L, 'sequence': 'CGATATCCAACCGCTAAATC'})
({'score': 100, 'version': 0L, 'sequence': 'TACTCACAGGTGCCAGTCAC'}, {'score': 97, 'version': 2L, 'sequence': 'TACTCACAGGTGCCAGTCAC'})
({'score': 99, 'version': 0L, 'sequence': 'AACAGGCTTTGTGTGCACTC'}, {'score': 100, 'version': 2L, 'sequence': 'AACAGGCTTTGTGTGCACTC'})
({'score': 99, 'version': 0L, 'sequence': 'CATTTTGCCCCCCCCAAGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CATTTTGCCCCCCCCAAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTATGGGGGGGCAAAATC'}, {'score': 80, 'version': 2L, 'sequence': 'TTTTATGGGGGGGCAAAATC'})
({'score': 97, 'version': 0L, 'sequence': 'ATGTTGAACCGCCCCTTTAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATGTTGAACCGCCCCTTTAC'})
({'score': 99, 'version': 0L, 'sequence': 'AAGACCAATTGTAAAGTTGC'}, {'score': 62, 'version': 2L, 'sequence': 'AAGACCAATTGTAAAGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTCATTCACACCACTGCC'}, {'score': 0, 'version': 2L, 'sequence': 'CTCTCATTCACACCACTGCC'})
({'score': 16, 'version': 0L, 'sequence': 'TGCTTAGGTAAACAAACCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCTTAGGTAAACAAACCCC'})
({'score': 1, 'version': 0L, 'sequence': 'TTCAGCAGCTATCCAGTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCAGCAGCTATCCAGTTGC'})
({'score': 97, 'version': 0L, 'sequence': 'GTGGTTTTTAAAAAATGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTGGTTTTTAAAAAATGCTC'})
({'score': 4, 'version': 0L, 'sequence': 'ACCTTTGAGTTAACTTTTAC'}, {'score': 0, 'version': 2L, 'sequence': 'ACCTTTGAGTTAACTTTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'}, {'score': 16, 'version': 2L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'})
({'score': 2, 'version': 0L, 'sequence': 'TGCTAAGGTAAGTTGCACCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCTAAGGTAAGTTGCACCC'})
({'score': 97, 'version': 0L, 'sequence': 'ATGTGTTTTCTGCCTGCTGC'}, {'score': 100, 'version': 2L, 'sequence': 'ATGTGTTTTCTGCCTGCTGC'})
({'score': 86, 'version': 0L, 'sequence': 'GAGGGGCCCCTCTCTGTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GAGGGGCCCCTCTCTGTTCC'})
({'score': 56, 'version': 0L, 'sequence': 'GCAGGGGATTTTCTGCCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCAGGGGATTTTCTGCCTTC'})
({'score': 98, 'version': 0L, 'sequence': 'TATAGGCATTATGGTTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TATAGGCATTATGGTTGCAC'})
({'score': 99, 'version': 0L, 'sequence': 'GGATTTCTACTGCAGCAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGATTTCTACTGCAGCAAGC'})
({'score': 93, 'version': 0L, 'sequence': 'ATGGGCCCAGGGTGACCAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATGGGCCCAGGGTGACCAAC'})
({'score': 84, 'version': 0L, 'sequence': 'TATCATCTATCTACCATCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TATCATCTATCTACCATCTC'})
({'score': 31, 'version': 0L, 'sequence': 'TAATAGAGTTGATAGATAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TAATAGAGTTGATAGATAGC'})
({'score': 99, 'version': 0L, 'sequence': 'AGTGAGACTTCACAGCTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTGAGACTTCACAGCTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGGGCTGGGATATAGTTGC'}, {'score': 87, 'version': 2L, 'sequence': 'CAGGGCTGGGATATAGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCATGGTAACCATGATTAAC'}, {'score': 95, 'version': 2L, 'sequence': 'CCATGGTAACCATGATTAAC'})
({'score': 42, 'version': 0L, 'sequence': 'ACTCCCTACCCAGAGTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCCCTACCCAGAGTGCAC'})
({'score': 42, 'version': 0L, 'sequence': 'ACTCCCTACCCAGAGTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCCCTACCCAGAGTGCAC'})
({'score': 30, 'version': 0L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'})
({'score': 97, 'version': 0L, 'sequence': 'GGGGGTGGGCAGCATGGGAC'}, {'score': 45, 'version': 2L, 'sequence': 'GGGGGTGGGCAGCATGGGAC'})
({'score': 30, 'version': 0L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'})
({'score': 30, 'version': 0L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'})
({'score': 30, 'version': 0L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'})
({'score': 50, 'version': 0L, 'sequence': 'GAGAGTGGGCAGTATGGGAC'}, {'score': 47, 'version': 2L, 'sequence': 'GAGAGTGGGCAGTATGGGAC'})
({'score': 30, 'version': 0L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCCCTACCCAGGGTGCAC'})
({'score': 99, 'version': 0L, 'sequence': 'AACAAAAAACTGAGTATGGC'}, {'score': 100, 'version': 2L, 'sequence': 'AACAAAAAACTGAGTATGGC'})
({'score': 0, 'version': 0L, 'sequence': 'TTTAGCAGCTATTTGGTTGC'}, {'score': 21, 'version': 2L, 'sequence': 'TTTAGCAGCTATTTGGTTGC'})
({'score': 0, 'version': 0L, 'sequence': 'TTTAGCAGCTATTTGGTTGC'}, {'score': 21, 'version': 2L, 'sequence': 'TTTAGCAGCTATTTGGTTGC'})
({'score': 93, 'version': 0L, 'sequence': 'TACAAGTAATGTTTACAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'TACAAGTAATGTTTACAGTC'})
({'score': 55, 'version': 0L, 'sequence': 'TGTAAACATTACTTGTAGAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTAAACATTACTTGTAGAC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 9, 'version': 0L, 'sequence': 'TAGACACTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACACTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGTGTC'}, {'score': 97, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGTGTC'})
({'score': 65, 'version': 0L, 'sequence': 'GTAGAGTTTAGTGTTTAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTAGAGTTTAGTGTTTAGTC'})
({'score': 94, 'version': 0L, 'sequence': 'TAAACACTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAAACACTAAACTCTACTTC'})
({'score': 41, 'version': 0L, 'sequence': 'TAGACTGTAAACATTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTGTAAACATTACTTC'})
({'score': 99, 'version': 0L, 'sequence': 'GAAGTAATGTTTACAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'GAAGTAATGTTTACAGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 41, 'version': 0L, 'sequence': 'TAGACTGTAAACATTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTGTAAACATTACTTC'})
({'score': 99, 'version': 0L, 'sequence': 'GAAGTAATGTTTACAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'GAAGTAATGTTTACAGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 41, 'version': 0L, 'sequence': 'TAGACTGTAAACATTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTGTAAACATTACTTC'})
({'score': 99, 'version': 0L, 'sequence': 'GAAGTAATGTTTACAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'GAAGTAATGTTTACAGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 95, 'version': 0L, 'sequence': 'TAGACACTAAAATCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACACTAAAATCTACTTC'})
({'score': 98, 'version': 0L, 'sequence': 'GAAGTAGATTTTAGTGTC'}, {'score': 99, 'version': 2L, 'sequence': 'GAAGTAGATTTTAGTGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 9, 'version': 0L, 'sequence': 'TAGACACTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACACTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGTGTC'}, {'score': 97, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGTGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 94, 'version': 0L, 'sequence': 'TAGACTCAGAACTCTACTTC'}, {'score': 63, 'version': 2L, 'sequence': 'TAGACTCAGAACTCTACTTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 94, 'version': 0L, 'sequence': 'TAGACTCAGAACTCTACTTC'}, {'score': 63, 'version': 2L, 'sequence': 'TAGACTCAGAACTCTACTTC'})
({'score': 95, 'version': 0L, 'sequence': 'GTAGAGTTTACAGTTTAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTAGAGTTTACAGTTTAGTC'})
({'score': 11, 'version': 0L, 'sequence': 'TAGACTCTAAACTCTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTCTAAACTCTACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGTAGAGTTTAGAGTC'}, {'score': 91, 'version': 2L, 'sequence': 'GAAGTAGAGTTTAGAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTCTCCTTTATTTAGAGCC'}, {'score': 97, 'version': 2L, 'sequence': 'CCTCTCCTTTATTTAGAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGTATCAGACTGGGCCTCC'}, {'score': 6, 'version': 2L, 'sequence': 'CAGTATCAGACTGGGCCTCC'})
({'score': 96, 'version': 0L, 'sequence': 'TCAGGTTCTCCAGTGGGCCC'}, {'score': 13, 'version': 2L, 'sequence': 'TCAGGTTCTCCAGTGGGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGACCCTGGGTAGAGCCAGC'}, {'score': 97, 'version': 2L, 'sequence': 'TGACCCTGGGTAGAGCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TACTTACTTAGCCTGGAACC'}, {'score': 99, 'version': 2L, 'sequence': 'TACTTACTTAGCCTGGAACC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGCCACACTGGGGCAGAAC'}, {'score': 41, 'version': 2L, 'sequence': 'CAGCCACACTGGGGCAGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACGCTGGGCAGATTGGCACC'}, {'score': 77, 'version': 2L, 'sequence': 'ACGCTGGGCAGATTGGCACC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGTTGTTATGGTTATTTCC'}, {'score': 99, 'version': 2L, 'sequence': 'TGGTTGTTATGGTTATTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGGGGGCTCAGTAGCAAGC'}, {'score': 99, 'version': 2L, 'sequence': 'TGGGGGGCTCAGTAGCAAGC'})
({'score': 98, 'version': 0L, 'sequence': 'CAACCTGCTAATATGGTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CAACCTGCTAATATGGTTTC'})
({'score': 68, 'version': 0L, 'sequence': 'ATGCTGATGGTGAGATCTCC'}, {'score': 99, 'version': 2L, 'sequence': 'ATGCTGATGGTGAGATCTCC'})
({'score': 99, 'version': 0L, 'sequence': 'AGAACACATTTAAGGAGACC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAACACATTTAAGGAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'TATAGGAAAAGGGGCAACCC'}, {'score': 99, 'version': 2L, 'sequence': 'TATAGGAAAAGGGGCAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCCATCACCCACATTTTCC'}, {'score': 99, 'version': 2L, 'sequence': 'GCCCATCACCCACATTTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTATTGGGCCCCGGCGGCC'}, {'score': 0, 'version': 2L, 'sequence': 'TCTATTGGGCCCCGGCGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTCTGCAAGGGTCGGGTC'}, {'score': 32, 'version': 2L, 'sequence': 'GTGTCTGCAAGGGTCGGGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAACCCGCCTCGCTTACAC'}, {'score': 33, 'version': 2L, 'sequence': 'GTAACCCGCCTCGCTTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 95, 'version': 0L, 'sequence': 'GTAACCCGCCTTCCCTACAC'}, {'score': 18, 'version': 2L, 'sequence': 'GTAACCCGCCTTCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCAGGTTACCTGGGGTAAC'}, {'score': 41, 'version': 2L, 'sequence': 'GGCAGGTTACCTGGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAACCTGCCTTCCCTACAC'}, {'score': 14, 'version': 2L, 'sequence': 'GTAACCTGCCTTCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAACCTGCCTCCCCTACAC'}, {'score': 50, 'version': 2L, 'sequence': 'GTAACCTGCCTCCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 95, 'version': 0L, 'sequence': 'GTAACCCGCCTTCCCTACAC'}, {'score': 18, 'version': 2L, 'sequence': 'GTAACCCGCCTTCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAACCCGCCTCGCTTACAC'}, {'score': 33, 'version': 2L, 'sequence': 'GTAACCCGCCTCGCTTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 95, 'version': 0L, 'sequence': 'GTAACCCGCCTTCCCTACAC'}, {'score': 18, 'version': 2L, 'sequence': 'GTAACCCGCCTTCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 95, 'version': 0L, 'sequence': 'GTAACCCGCCTTCCCTACAC'}, {'score': 18, 'version': 2L, 'sequence': 'GTAACCCGCCTTCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAACCCGCCTCGCTTACAC'}, {'score': 33, 'version': 2L, 'sequence': 'GTAACCCGCCTCGCTTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 95, 'version': 0L, 'sequence': 'GTAACCCGCCTTCCCTACAC'}, {'score': 18, 'version': 2L, 'sequence': 'GTAACCCGCCTTCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCAGGTTACCTGGGGTAAC'}, {'score': 41, 'version': 2L, 'sequence': 'GGCAGGTTACCTGGGGTAAC'})
({'score': 95, 'version': 0L, 'sequence': 'GTAACCCGCCTTCCCTACAC'}, {'score': 18, 'version': 2L, 'sequence': 'GTAACCCGCCTTCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAACCCGCCTCGCTTACAC'}, {'score': 33, 'version': 2L, 'sequence': 'GTAACCCGCCTCGCTTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCAGGTTACCTGGGGTAAC'}, {'score': 41, 'version': 2L, 'sequence': 'GGCAGGTTACCTGGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAACCTGCCTTCCCTACAC'}, {'score': 14, 'version': 2L, 'sequence': 'GTAACCTGCCTTCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 95, 'version': 0L, 'sequence': 'GTAACCCGCCTTCCCTACAC'}, {'score': 18, 'version': 2L, 'sequence': 'GTAACCCGCCTTCCCTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAACCCGCCTTGCTTACAC'}, {'score': 33, 'version': 2L, 'sequence': 'GTAACCCGCCTTGCTTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'}, {'score': 11, 'version': 2L, 'sequence': 'GGCGGGTTACCTGGGGTAAC'})
({'score': 16, 'version': 0L, 'sequence': 'TTTATACATATGGGTATGGC'}, {'score': 96, 'version': 2L, 'sequence': 'TTTATACATATGGGTATGGC'})
({'score': 98, 'version': 0L, 'sequence': 'TAGGAGCTGCCATATTGC'}, {'score': 99, 'version': 2L, 'sequence': 'TAGGAGCTGCCATATTGC'})
({'score': 99, 'version': 0L, 'sequence': 'GCTGTAATATTATACTGTAC'}, {'score': 95, 'version': 2L, 'sequence': 'GCTGTAATATTATACTGTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTCATAATTTACAAAGTGC'}, {'score': 97, 'version': 2L, 'sequence': 'TTTCATAATTTACAAAGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CGGACCAGCCCACACATCAC'}, {'score': 24, 'version': 2L, 'sequence': 'CGGACCAGCCCACACATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTAAGGGCATCAGACAGAC'}, {'score': 54, 'version': 2L, 'sequence': 'TGTAAGGGCATCAGACAGAC'})
({'score': 95, 'version': 0L, 'sequence': 'CTGTATGACTAACCTTGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGTATGACTAACCTTGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATGGCAGCTGAGAGAGCTTC'}, {'score': 65, 'version': 2L, 'sequence': 'ATGGCAGCTGAGAGAGCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAGCTTCCCGCTGCAGCTC'}, {'score': 0, 'version': 2L, 'sequence': 'GTAGCTTCCCGCTGCAGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'TAATCTGAGATACCAATAGC'}, {'score': 17, 'version': 2L, 'sequence': 'TAATCTGAGATACCAATAGC'})
({'score': 70, 'version': 0L, 'sequence': 'TGACCCCCGCAGCCAAAACC'}, {'score': 49, 'version': 2L, 'sequence': 'TGACCCCCGCAGCCAAAACC'})
({'score': 47, 'version': 0L, 'sequence': 'ACTGGAGCCTCACAGAGCAC'}, {'score': 0, 'version': 2L, 'sequence': 'ACTGGAGCCTCACAGAGCAC'})
({'score': 97, 'version': 0L, 'sequence': 'TGCTGGGTGAAATTGGACCC'}, {'score': 9, 'version': 2L, 'sequence': 'TGCTGGGTGAAATTGGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGAAACATTTCGCCCATCAC'}, {'score': 0, 'version': 2L, 'sequence': 'GGAAACATTTCGCCCATCAC'})
({'score': 1, 'version': 0L, 'sequence': 'ATAGATAGATAGATAGATGC'}, {'score': 0, 'version': 2L, 'sequence': 'ATAGATAGATAGATAGATGC'})
({'score': 1, 'version': 0L, 'sequence': 'ATAGATAGATAGATAGATGC'}, {'score': 0, 'version': 2L, 'sequence': 'ATAGATAGATAGATAGATGC'})
({'score': 1, 'version': 0L, 'sequence': 'ATAGATAGATAGATAGATGC'}, {'score': 0, 'version': 2L, 'sequence': 'ATAGATAGATAGATAGATGC'})
({'score': 1, 'version': 0L, 'sequence': 'ATAGATAGATAGATAGATGC'}, {'score': 0, 'version': 2L, 'sequence': 'ATAGATAGATAGATAGATGC'})
({'score': 1, 'version': 0L, 'sequence': 'ATAGATAGATAGATAGATGC'}, {'score': 0, 'version': 2L, 'sequence': 'ATAGATAGATAGATAGATGC'})
({'score': 85, 'version': 0L, 'sequence': 'CCCCTAAATGTTTGCCGCCC'}, {'score': 1, 'version': 2L, 'sequence': 'CCCCTAAATGTTTGCCGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTCATTCACACCACTGCC'}, {'score': 0, 'version': 2L, 'sequence': 'CTCTCATTCACACCACTGCC'})
({'score': 2, 'version': 0L, 'sequence': 'TGCTAAGGTAAGTTGCACCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCTAAGGTAAGTTGCACCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAATTTGATCATTTGGCCC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATTTGATCATTTGGCCC'})
({'score': 20, 'version': 0L, 'sequence': 'GCTGGGGACCTGGGGTTTTC'}, {'score': 0, 'version': 2L, 'sequence': 'GCTGGGGACCTGGGGTTTTC'})
({'score': 0, 'version': 0L, 'sequence': 'GGGACAGATTCTCTCATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGACAGATTCTCTCATCAC'})
({'score': 1, 'version': 0L, 'sequence': 'TGTTAGGTTAAATTGGACCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTTAGGTTAAATTGGACCC'})
({'score': 0, 'version': 0L, 'sequence': 'TTCAGCAGATATTTGGTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCAGCAGATATTTGGTTGC'})
({'score': 39, 'version': 0L, 'sequence': 'TTATTACTTAACTTGTATTC'}, {'score': 100, 'version': 2L, 'sequence': 'TTATTACTTAACTTGTATTC'})
({'score': 97, 'version': 0L, 'sequence': 'TTTATAGATATTGACAAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTATAGATATTGACAAGTC'})
({'score': 99, 'version': 0L, 'sequence': 'TTTTTTAATTGCTTGATGGC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTTTTAATTGCTTGATGGC'})
({'score': 72, 'version': 0L, 'sequence': 'TTTATCTGAGGGTTTTATTC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTATCTGAGGGTTTTATTC'})
({'score': 95, 'version': 0L, 'sequence': 'GGTGAATAGAAACTGAATTC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTGAATAGAAACTGAATTC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACTTACACCCTGCAGATC'}, {'score': 95, 'version': 2L, 'sequence': 'ACACTTACACCCTGCAGATC'})
({'score': 0, 'version': 0L, 'sequence': 'GGGTTGGGGAATGCCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGTTGGGGAATGCCCTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCCACCTCCTTTTGAGTTC'}, {'score': 68, 'version': 2L, 'sequence': 'TTCCACCTCCTTTTGAGTTC'})
({'score': 64, 'version': 0L, 'sequence': 'ACAGGGTCCATAGAGCCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'ACAGGGTCCATAGAGCCCTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAAGAGCTTTATACAGTTC'}, {'score': 46, 'version': 2L, 'sequence': 'ATAAGAGCTTTATACAGTTC'})
({'score': 95, 'version': 0L, 'sequence': 'ACCTAAAGACATTACTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCTAAAGACATTACTGCAC'})
({'score': 32, 'version': 0L, 'sequence': 'GGCCTAAGATTGACCAGATC'}, {'score': 2, 'version': 2L, 'sequence': 'GGCCTAAGATTGACCAGATC'})
({'score': 14, 'version': 0L, 'sequence': 'AAAATCAAACCTGCCCGGTC'}, {'score': 85, 'version': 2L, 'sequence': 'AAAATCAAACCTGCCCGGTC'})
({'score': 78, 'version': 0L, 'sequence': 'CCTTTAGGTTGAGCACAACC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTTTAGGTTGAGCACAACC'})
({'score': 94, 'version': 0L, 'sequence': 'AGTCCGACACTGTTCCAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTCCGACACTGTTCCAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGTGCAGATTGGCTGGTGC'}, {'score': 50, 'version': 2L, 'sequence': 'AAGTGCAGATTGGCTGGTGC'})
({'score': 65, 'version': 0L, 'sequence': 'GATCCACTTGTTTAGTCACC'}, {'score': 100, 'version': 2L, 'sequence': 'GATCCACTTGTTTAGTCACC'})
({'score': 57, 'version': 0L, 'sequence': 'CGAAGAATCCCTCGCTTGGC'}, {'score': 100, 'version': 2L, 'sequence': 'CGAAGAATCCCTCGCTTGGC'})
({'score': 97, 'version': 0L, 'sequence': 'GATTGAAAATTATTTATTGC'}, {'score': 100, 'version': 2L, 'sequence': 'GATTGAAAATTATTTATTGC'})
({'score': 95, 'version': 0L, 'sequence': 'CAATTACTTATTTAAATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'CAATTACTTATTTAAATCAC'})
({'score': 37, 'version': 0L, 'sequence': 'TAGAGTGGGTACCGGACCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGAGTGGGTACCGGACCAC'})
({'score': 33, 'version': 0L, 'sequence': 'GAAGCCTGTTACACTCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GAAGCCTGTTACACTCTTCC'})
({'score': 18, 'version': 0L, 'sequence': 'GCTATTACCCCCCTCTAACC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTATTACCCCCCTCTAACC'})
({'score': 91, 'version': 0L, 'sequence': 'CAGTAGAGGAATAGCCAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGTAGAGGAATAGCCAGTC'})
({'score': 29, 'version': 0L, 'sequence': 'AAAGTAACTATGCTGCCACC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAGTAACTATGCTGCCACC'})
({'score': 17, 'version': 0L, 'sequence': 'TTGTTGCTTTGTTTGACCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGTTGCTTTGTTTGACCAC'})
({'score': 47, 'version': 0L, 'sequence': 'TCTGCCAACAACGTAGTACC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTGCCAACAACGTAGTACC'})
({'score': 99, 'version': 0L, 'sequence': 'TCCCCTTTCTACCAGTAACC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCCCTTTCTACCAGTAACC'})
({'score': 94, 'version': 0L, 'sequence': 'TTGGCACAGATAAGTGCAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGGCACAGATAAGTGCAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGGGGTGACCCAAAAGTTC'}, {'score': 99, 'version': 2L, 'sequence': 'TGGGGGTGACCCAAAAGTTC'})
({'score': 17, 'version': 0L, 'sequence': 'GGTATCCAGAATAAGTGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTATCCAGAATAAGTGGGC'})
({'score': 9, 'version': 0L, 'sequence': 'ACCAACCTCAAAACACGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCAACCTCAAAACACGCAC'})
({'score': 66, 'version': 0L, 'sequence': 'GCTATTGCGTCCCATAGGTC'}, {'score': 40, 'version': 2L, 'sequence': 'GCTATTGCGTCCCATAGGTC'})
({'score': 68, 'version': 0L, 'sequence': 'TTATCCAGGTTTTAAACTCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTATCCAGGTTTTAAACTCC'})
({'score': 97, 'version': 0L, 'sequence': 'ACATCACCAGCTCCACCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'ACATCACCAGCTCCACCCCC'})
({'score': 95, 'version': 0L, 'sequence': 'CATTTACAAATCTGAATTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CATTTACAAATCTGAATTTC'})
({'score': 98, 'version': 0L, 'sequence': 'CAGTTTTAGCAGGAACTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGTTTTAGCAGGAACTTTC'})
({'score': 99, 'version': 0L, 'sequence': 'AATGACCCCCCCCCTGTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AATGACCCCCCCCCTGTTTC'})
({'score': 96, 'version': 0L, 'sequence': 'CACTAACATTGAAATAAGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CACTAACATTGAAATAAGCC'})
({'score': 17, 'version': 0L, 'sequence': 'TCGTAAATCTCGAAACTATC'}, {'score': 100, 'version': 2L, 'sequence': 'TCGTAAATCTCGAAACTATC'})
({'score': 18, 'version': 0L, 'sequence': 'GTCAATTTTTCTTTTACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTCAATTTTTCTTTTACTTC'})
({'score': 35, 'version': 0L, 'sequence': 'TTGCTAAGTTATTTGGATCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGCTAAGTTATTTGGATCC'})
({'score': 1, 'version': 0L, 'sequence': 'TCTGTTTTAGTAGCTGCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTGTTTTAGTAGCTGCTAC'})
({'score': 54, 'version': 0L, 'sequence': 'GGGAAGCCACACACAGCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGAAGCCACACACAGCTAC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTCAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAGATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTTAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTTAGATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 64, 'version': 0L, 'sequence': 'TGCCACCTTCAGATCTGAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAGATCTGAAC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTCAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAGATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTCAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAGATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 20, 'version': 0L, 'sequence': 'TGCCACCTTCAAATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAAATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTCAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAGATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTTAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTTAGATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTCAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAGATCTGAGC'})
({'score': 66, 'version': 0L, 'sequence': 'CCATTAGATTGCACGTTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCACGTTCTC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTTAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTTAGATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTCAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAGATCTGAGC'})
({'score': 66, 'version': 0L, 'sequence': 'CCATTAGATTGCACGTTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCACGTTCTC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTTAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTTAGATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 64, 'version': 0L, 'sequence': 'TGCCACCTTCAGATCTGAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAGATCTGAAC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 11, 'version': 0L, 'sequence': 'TGCCACCTTCAGATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAGATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 20, 'version': 0L, 'sequence': 'TGCCACCTTCAAATCTGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCACCTTCAAATCTGAGC'})
({'score': 14, 'version': 0L, 'sequence': 'CCATTAGATTGCAAGTTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATTAGATTGCAAGTTCCC'})
({'score': 54, 'version': 0L, 'sequence': 'GCTTGAACATCCCATAATAC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTTGAACATCCCATAATAC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 93, 'version': 0L, 'sequence': 'AATAAGCCGGCACTCACGTC'}, {'score': 100, 'version': 2L, 'sequence': 'AATAAGCCGGCACTCACGTC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTGATCATTTTGTAGGTCC'}, {'score': 96, 'version': 2L, 'sequence': 'ACTGATCATTTTGTAGGTCC'})
({'score': 99, 'version': 0L, 'sequence': 'GGGCAGCCATGATTGCTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGCAGCCATGATTGCTCCC'})
({'score': 99, 'version': 0L, 'sequence': 'TTTCCATCTAAAAAAATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTCCATCTAAAAAAATTCC'})
({'score': 39, 'version': 0L, 'sequence': 'TCAAATCAGTGCGTAGTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'TCAAATCAGTGCGTAGTTGC'})
({'score': 97, 'version': 0L, 'sequence': 'TAATAAAGGGGAACTATTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TAATAAAGGGGAACTATTAC'})
({'score': 97, 'version': 0L, 'sequence': 'TTTAACCAGCCCCTGGTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTAACCAGCCCCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGTGAAGCAGATGTAGGGC'}, {'score': 97, 'version': 2L, 'sequence': 'AAGTGAAGCAGATGTAGGGC'})
({'score': 1, 'version': 0L, 'sequence': 'CTGCAATACTTACTGATATC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGCAATACTTACTGATATC'})
({'score': 96, 'version': 0L, 'sequence': 'CCCCCCCCCGCAGGGCTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CCCCCCCCCGCAGGGCTTTC'})
({'score': 66, 'version': 0L, 'sequence': 'ATCAAGCTGCCATGCATGCC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCAAGCTGCCATGCATGCC'})
({'score': 66, 'version': 0L, 'sequence': 'GGCGCAGTACTGAGCAATGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGCGCAGTACTGAGCAATGC'})
({'score': 0, 'version': 0L, 'sequence': 'GTTATAAAATGGCCTATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTATAAAATGGCCTATTCC'})
({'score': 0, 'version': 0L, 'sequence': 'AAAAACTATTTAATGTCAGC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAAACTATTTAATGTCAGC'})
({'score': 0, 'version': 0L, 'sequence': 'ATACAGTTCTAAAGAATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATACAGTTCTAAAGAATCAC'})
({'score': 97, 'version': 0L, 'sequence': 'GATTTCCATGTGGGTTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GATTTCCATGTGGGTTTTTC'})
({'score': 66, 'version': 0L, 'sequence': 'ATCAAGCTGCCATGCATGCC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCAAGCTGCCATGCATGCC'})
({'score': 66, 'version': 0L, 'sequence': 'GGCGCAGTACTGAGCAATGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGCGCAGTACTGAGCAATGC'})
({'score': 97, 'version': 0L, 'sequence': 'TATTAGGTACCTACAGTACC'}, {'score': 100, 'version': 2L, 'sequence': 'TATTAGGTACCTACAGTACC'})
({'score': 10, 'version': 0L, 'sequence': 'GTGGAGGGCCAGAGATCCAC'}, {'score': 100, 'version': 2L, 'sequence': 'GTGGAGGGCCAGAGATCCAC'})
({'score': 16, 'version': 0L, 'sequence': 'CTGGGGCTTAGTGATGTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGGGGCTTAGTGATGTTTC'})
({'score': 9, 'version': 0L, 'sequence': 'GATGGCAAGAAGGAGGAGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GATGGCAAGAAGGAGGAGCC'})
({'score': 50, 'version': 0L, 'sequence': 'CAGACAAGCCAAACCCACAC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGACAAGCCAAACCCACAC'})
({'score': 10, 'version': 0L, 'sequence': 'AAACCACCATAATCCCTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAACCACCATAATCCCTCTC'})
({'score': 13, 'version': 0L, 'sequence': 'CTCGCAAAGGTGCTTTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTCGCAAAGGTGCTTTTTTC'})
({'score': 5, 'version': 0L, 'sequence': 'GCCTGAAAACAAAATCAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCTGAAAACAAAATCAGTC'})
({'score': 8, 'version': 0L, 'sequence': 'GGAATAGCAAAGTAGACATC'}, {'score': 100, 'version': 2L, 'sequence': 'GGAATAGCAAAGTAGACATC'})
({'score': 0, 'version': 0L, 'sequence': 'AGTTTGGGGAATGCCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTTTGGGGAATGCCCTTCC'})
({'score': 1, 'version': 0L, 'sequence': 'TGCTTGTTCAAGAACTCATC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCTTGTTCAAGAACTCATC'})
({'score': 1, 'version': 0L, 'sequence': 'CTAATATCTACAGTTAGTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATATCTACAGTTAGTAC'})
({'score': 99, 'version': 0L, 'sequence': 'ATTGGGGGGATGGAGGGGAC'}, {'score': 93, 'version': 2L, 'sequence': 'ATTGGGGGGATGGAGGGGAC'})
({'score': 20, 'version': 0L, 'sequence': 'ACTCATTACAGCTGCGCTGC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCATTACAGCTGCGCTGC'})
({'score': 97, 'version': 0L, 'sequence': 'AGAGCAATTAGGAAATATAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAGCAATTAGGAAATATAC'})
({'score': 100, 'version': 0L, 'sequence': 'TAATAGGGTGCCAGTAGCAC'}, {'score': 94, 'version': 2L, 'sequence': 'TAATAGGGTGCCAGTAGCAC'})
({'score': 96, 'version': 0L, 'sequence': 'GACACGCCACTCCCAGGCGC'}, {'score': 75, 'version': 2L, 'sequence': 'GACACGCCACTCCCAGGCGC'})
({'score': 12, 'version': 0L, 'sequence': 'CTGTGTAAAGGCCAATTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGTGTAAAGGCCAATTAAC'})
({'score': 5, 'version': 0L, 'sequence': 'TAGTATTTCCCTGGCATGTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGTATTTCCCTGGCATGTC'})
({'score': 26, 'version': 0L, 'sequence': 'GACACTGTTATACAGATAGC'}, {'score': 100, 'version': 2L, 'sequence': 'GACACTGTTATACAGATAGC'})
({'score': 99, 'version': 0L, 'sequence': 'CTGGCCAAGCCTATGGCTGC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGGCCAAGCCTATGGCTGC'})
({'score': 77, 'version': 0L, 'sequence': 'TTATTAGCCCATTGGTCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTATTAGCCCATTGGTCCCC'})
({'score': 0, 'version': 0L, 'sequence': 'TGCTAAGCTAACTTGGACCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCTAAGCTAACTTGGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTGGCCATTGGCCTGAGTCC'}, {'score': 94, 'version': 2L, 'sequence': 'TTGGCCATTGGCCTGAGTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTCGCTCTCCTATTGGCCC'}, {'score': 95, 'version': 2L, 'sequence': 'TGTCGCTCTCCTATTGGCCC'})
({'score': 97, 'version': 0L, 'sequence': 'CAGGGCTGGGGCCCCAGTAC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGGGCTGGGGCCCCAGTAC'})
({'score': 98, 'version': 0L, 'sequence': 'GCCTAATGGGGAACAGGAC'}, {'score': 69, 'version': 2L, 'sequence': 'GCCTAATGGGGAACAGGAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTATAAAGAGAGGAAAATC'}, {'score': 83, 'version': 2L, 'sequence': 'AGTATAAAGAGAGGAAAATC'})
({'score': 21, 'version': 0L, 'sequence': 'GGGTAACAGTCGCCCCACTC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGTAACAGTCGCCCCACTC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCCAAGGTCCCGAGCATTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCAAGGTCCCGAGCATTC'})
({'score': 3, 'version': 0L, 'sequence': 'GTGACAACACACGGAGCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'GTGACAACACACGGAGCTAC'})
({'score': 2, 'version': 0L, 'sequence': 'TCTGTTTCTGTAGCTGCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTGTTTCTGTAGCTGCTAC'})
({'score': 98, 'version': 0L, 'sequence': 'TGTGTGGGGCAGTTTGCAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTGTGGGGCAGTTTGCAAC'})
({'score': 40, 'version': 0L, 'sequence': 'TATCAGGGCCAACATTGTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TATCAGGGCCAACATTGTAC'})
({'score': 37, 'version': 0L, 'sequence': 'ACAGTGAGGCGGGGCAAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACAGTGAGGCGGGGCAAAAC'})
({'score': 33, 'version': 0L, 'sequence': 'GTAACTAAAACAAGACCCAC'}, {'score': 100, 'version': 2L, 'sequence': 'GTAACTAAAACAAGACCCAC'})
({'score': 41, 'version': 0L, 'sequence': 'TTGTCTCCATCCCACCCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGTCTCCATCCCACCCCTC'})
({'score': 50, 'version': 0L, 'sequence': 'GCCATTGTTGCCGATGGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCATTGTTGCCGATGGAGC'})
({'score': 50, 'version': 0L, 'sequence': 'CCCAGTGATACACTGACCGC'}, {'score': 100, 'version': 2L, 'sequence': 'CCCAGTGATACACTGACCGC'})
({'score': 49, 'version': 0L, 'sequence': 'TTGCTGTAAGACATGAGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGCTGTAAGACATGAGGCC'})
({'score': 66, 'version': 0L, 'sequence': 'GTTACCAACATGGCGCTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTACCAACATGGCGCTCCC'})
({'score': 95, 'version': 0L, 'sequence': 'CTCGCCCCCTCCATGGTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'CTCGCCCCCTCCATGGTTGC'})
({'score': 49, 'version': 0L, 'sequence': 'TTGCTGTAAGACATGAGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGCTGTAAGACATGAGGCC'})
({'score': 50, 'version': 0L, 'sequence': 'CCCAGTGATACACTGACCGC'}, {'score': 100, 'version': 2L, 'sequence': 'CCCAGTGATACACTGACCGC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 0, 'version': 0L, 'sequence': 'AAGGTTGGGGACCCCTGCTC'}, {'score': 8, 'version': 2L, 'sequence': 'AAGGTTGGGGACCCCTGCTC'})
({'score': 2, 'version': 0L, 'sequence': 'CTTGTTATCAACAAAACCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTTGTTATCAACAAAACCCC'})
({'score': 0, 'version': 0L, 'sequence': 'CAAATTGAGGAATATTGGCC'}, {'score': 87, 'version': 2L, 'sequence': 'CAAATTGAGGAATATTGGCC'})
({'score': 0, 'version': 0L, 'sequence': 'CCTTGTAATATGCCCAGTGC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTTGTAATATGCCCAGTGC'})
({'score': 11, 'version': 0L, 'sequence': 'CTCTGAACCTACGTGACAGC'}, {'score': 100, 'version': 2L, 'sequence': 'CTCTGAACCTACGTGACAGC'})
({'score': 12, 'version': 0L, 'sequence': 'TCCATGCTCATACGCAACAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCATGCTCATACGCAACAC'})
({'score': 6, 'version': 0L, 'sequence': 'AGGGGGGGACCGCCAGCCAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGGGGGGGACCGCCAGCCAC'})
({'score': 18, 'version': 0L, 'sequence': 'ACCAACCTGTATCCCATATC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCAACCTGTATCCCATATC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGTACATGGGCACTCTTCC'}, {'score': 96, 'version': 2L, 'sequence': 'CTGTACATGGGCACTCTTCC'})
({'score': 46, 'version': 0L, 'sequence': 'TCAGCTTCCCTGGGGGCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TCAGCTTCCCTGGGGGCCCC'})
({'score': 39, 'version': 0L, 'sequence': 'CAGTGTCGGACTGCGCCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGTGTCGGACTGCGCCCCC'})
({'score': 95, 'version': 0L, 'sequence': 'TTATTGACTGTGGATTTTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTATTGACTGTGGATTTTAC'})
({'score': 99, 'version': 0L, 'sequence': 'TAATAAGTGTAAATGGCCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TAATAAGTGTAAATGGCCAC'})
({'score': 98, 'version': 0L, 'sequence': 'CACATTCATATAGGGGGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CACATTCATATAGGGGGGCC'})
({'score': 85, 'version': 0L, 'sequence': 'CAGTGGGGCCCTCCTACTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGTGGGGCCCTCCTACTCC'})
({'score': 97, 'version': 0L, 'sequence': 'TAACCTGCAGAGCCAGCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'TAACCTGCAGAGCCAGCTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 17, 'version': 0L, 'sequence': 'CGTAACCTGCAGAGCATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAGCATTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 25, 'version': 0L, 'sequence': 'CGTAAACTGCAGAACATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAAACTGCAGAACATTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 25, 'version': 0L, 'sequence': 'CGTAAACTGCAGAACATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAAACTGCAGAACATTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 17, 'version': 0L, 'sequence': 'CGTAACCTGCAGAGCATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAGCATTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 17, 'version': 0L, 'sequence': 'CGTAACCTGCAGAGCATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAGCATTCC'})
({'score': 12, 'version': 0L, 'sequence': 'TGTAACCTGCAGAGCATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTAACCTGCAGAGCATTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 20, 'version': 0L, 'sequence': 'CGTAACCTGCAGAACATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAACATTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 17, 'version': 0L, 'sequence': 'CGTAACCTGCAGAGCATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAGCATTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 17, 'version': 0L, 'sequence': 'CGTAACCTGCAGAGCATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAGCATTCC'})
({'score': 66, 'version': 0L, 'sequence': 'GCCCTGGGACACCCCGGATC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCTGGGACACCCCGGATC'})
({'score': 20, 'version': 0L, 'sequence': 'CGTAACCTGCAGAACATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAACATTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 20, 'version': 0L, 'sequence': 'CGTAACCTGCAGAACATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAACATTCC'})
({'score': 27, 'version': 0L, 'sequence': 'GCCCCGGGACACCCTGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCTGGCTC'})
({'score': 17, 'version': 0L, 'sequence': 'CGTAACCTGCAGAGCATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAGCATTCC'})
({'score': 13, 'version': 0L, 'sequence': 'GCCCCGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCCGGGACACCCCGGCTC'})
({'score': 17, 'version': 0L, 'sequence': 'CGTAACCTGCAGAGCATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAGCATTCC'})
({'score': 66, 'version': 0L, 'sequence': 'GCCCTGGGACACCCCGGATC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCTGGGACACCCCGGATC'})
({'score': 20, 'version': 0L, 'sequence': 'CGTAACCTGCAGAACATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGTAACCTGCAGAACATTCC'})
({'score': 8, 'version': 0L, 'sequence': 'GCCCTGGGACACCCCGGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCTGGGACACCCCGGCTC'})
({'score': 99, 'version': 0L, 'sequence': 'TGTACTGAGTCGCATCCAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTACTGAGTCGCATCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGAAGGCAAGACACAAGAC'}, {'score': 99, 'version': 2L, 'sequence': 'GAGAAGGCAAGACACAAGAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTCACAGCAGCCAATAGGC'}, {'score': 99, 'version': 2L, 'sequence': 'TGTCACAGCAGCCAATAGGC'})
({'score': 96, 'version': 0L, 'sequence': 'CTATAGTTCCAGGGTGATGC'}, {'score': 100, 'version': 2L, 'sequence': 'CTATAGTTCCAGGGTGATGC'})
({'score': 98, 'version': 0L, 'sequence': 'AGTAGGCCGGTGCCTGCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTAGGCCGGTGCCTGCTCC'})
({'score': 47, 'version': 0L, 'sequence': 'TGGGCACGGTTCTGCCCAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGGCACGGTTCTGCCCAAC'})
({'score': 70, 'version': 0L, 'sequence': 'GACTTATTAGAAAGGGTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'GACTTATTAGAAAGGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCCAGCCAAGGACACCTTC'}, {'score': 64, 'version': 2L, 'sequence': 'CCCCAGCCAAGGACACCTTC'})
({'score': 36, 'version': 0L, 'sequence': 'AGAATGCATATTTGCTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAATGCATATTTGCTTTTC'})
({'score': 99, 'version': 0L, 'sequence': 'AGAGGGAAATCACATGAGAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAGGGAAATCACATGAGAC'})
({'score': 70, 'version': 0L, 'sequence': 'AGGTGCAAAGTAAAGGGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGGTGCAAAGTAAAGGGCAC'})
({'score': 6, 'version': 0L, 'sequence': 'AGTACAGGGGAATCCCTATC'}, {'score': 44, 'version': 2L, 'sequence': 'AGTACAGGGGAATCCCTATC'})
({'score': 95, 'version': 0L, 'sequence': 'AATAAAGGGATTGGTAGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'AATAAAGGGATTGGTAGCAC'})
({'score': 98, 'version': 0L, 'sequence': 'GGGGTTAATTGGCTGACCCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGGTTAATTGGCTGACCCC'})
({'score': 40, 'version': 0L, 'sequence': 'GGGCAATGGTAAATGAGCCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGCAATGGTAAATGAGCCC'})
({'score': 96, 'version': 0L, 'sequence': 'CAGACAGATCCTACTCTCAC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGACAGATCCTACTCTCAC'})
({'score': 97, 'version': 0L, 'sequence': 'TCTGTGTGAAATGTAATTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTGTGTGAAATGTAATTAC'})
({'score': 10, 'version': 0L, 'sequence': 'GCAGCTTATAGGGGAGGGGC'}, {'score': 5, 'version': 2L, 'sequence': 'GCAGCTTATAGGGGAGGGGC'})
({'score': 3, 'version': 0L, 'sequence': 'ACTCAGGCAGTGGGCTGGAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCAGGCAGTGGGCTGGAC'})
({'score': 92, 'version': 0L, 'sequence': 'AAGCTGCCATCAGAGTGACC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGCTGCCATCAGAGTGACC'})
({'score': 41, 'version': 0L, 'sequence': 'AGGGGGCGGAGCTGGGAATC'}, {'score': 29, 'version': 2L, 'sequence': 'AGGGGGCGGAGCTGGGAATC'})
({'score': 84, 'version': 0L, 'sequence': 'GGGGGGGGGTCCTGCCAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGGGGGGGTCCTGCCAAGC'})
({'score': 99, 'version': 0L, 'sequence': 'AATTTTCCTACCCCCTCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'AATTTTCCTACCCCCTCCCC'})
({'score': 58, 'version': 0L, 'sequence': 'GGGGGGGGGGGAACTGTCAC'}, {'score': 12, 'version': 2L, 'sequence': 'GGGGGGGGGGGAACTGTCAC'})
({'score': 97, 'version': 0L, 'sequence': 'TGGCTTGCAAACAGACAGGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGCTTGCAAACAGACAGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAACTGGGGGGGTTTTCCC'}, {'score': 97, 'version': 2L, 'sequence': 'TAAACTGGGGGGGTTTTCCC'})
({'score': 98, 'version': 0L, 'sequence': 'GGGGTTTAAAAATTATTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGGTTTAAAAATTATTTTC'})
({'score': 97, 'version': 0L, 'sequence': 'AGGTAGCACAGACTGAAGGC'}, {'score': 95, 'version': 2L, 'sequence': 'AGGTAGCACAGACTGAAGGC'})
({'score': 96, 'version': 0L, 'sequence': 'TGCCAGTCCTTATAGGGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCAGTCCTTATAGGGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGAGAGTGAGAATGGGTTC'}, {'score': 95, 'version': 2L, 'sequence': 'CTGAGAGTGAGAATGGGTTC'})
({'score': 99, 'version': 0L, 'sequence': 'CTGGGCAGGCCTGGACTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGGGCAGGCCTGGACTAAC'})
({'score': 33, 'version': 0L, 'sequence': 'CGATGTGGCCCTGATCCGAC'}, {'score': 100, 'version': 2L, 'sequence': 'CGATGTGGCCCTGATCCGAC'})
({'score': 10, 'version': 0L, 'sequence': 'CCCCATACACGGGCCAATCC'}, {'score': 50, 'version': 2L, 'sequence': 'CCCCATACACGGGCCAATCC'})
({'score': 93, 'version': 0L, 'sequence': 'TTTATTTCTGTTACTTTTTC'}, {'score': 95, 'version': 2L, 'sequence': 'TTTATTTCTGTTACTTTTTC'})
({'score': 0, 'version': 0L, 'sequence': 'GATGTGGTCCCCAATCCGAC'}, {'score': 100, 'version': 2L, 'sequence': 'GATGTGGTCCCCAATCCGAC'})
({'score': 51, 'version': 0L, 'sequence': 'ACGGGCCTGGTTAGAAAATC'}, {'score': 100, 'version': 2L, 'sequence': 'ACGGGCCTGGTTAGAAAATC'})
({'score': 48, 'version': 0L, 'sequence': 'AGTATATAATACAGCATTGC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTATATAATACAGCATTGC'})
({'score': 50, 'version': 0L, 'sequence': 'AAAAGCATTGAAATCCACCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAAGCATTGAAATCCACCC'})
({'score': 97, 'version': 0L, 'sequence': 'CTGTATGTTTTAGGCTTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGTATGTTTTAGGCTTCTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCATAGGAAGGGGTTACCAC'}, {'score': 99, 'version': 2L, 'sequence': 'GCATAGGAAGGGGTTACCAC'})
({'score': 36, 'version': 0L, 'sequence': 'CCCCCCCCCCCCCTACATTC'}, {'score': 100, 'version': 2L, 'sequence': 'CCCCCCCCCCCCCTACATTC'})
({'score': 17, 'version': 0L, 'sequence': 'AGAAATAGGGGTTGTAGCAC'}, {'score': 16, 'version': 2L, 'sequence': 'AGAAATAGGGGTTGTAGCAC'})
({'score': 99, 'version': 0L, 'sequence': 'TTGTTTTACATTGCTCCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGTTTTACATTGCTCCCCC'})
({'score': 99, 'version': 0L, 'sequence': 'CCTGGAACACTTGTTGAGAC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTGGAACACTTGTTGAGAC'})
({'score': 0, 'version': 0L, 'sequence': 'TCCTGGATTCAGTGCATCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCTGGATTCAGTGCATCCC'})
({'score': 97, 'version': 0L, 'sequence': 'TTATCATTGTAAATGGCCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTATCATTGTAAATGGCCAC'})
({'score': 12, 'version': 0L, 'sequence': 'ATTCACCTACTTTATAGGTC'}, {'score': 100, 'version': 2L, 'sequence': 'ATTCACCTACTTTATAGGTC'})
({'score': 22, 'version': 0L, 'sequence': 'CGCCATATGGGATACGTGGC'}, {'score': 17, 'version': 2L, 'sequence': 'CGCCATATGGGATACGTGGC'})
({'score': 17, 'version': 0L, 'sequence': 'TTTTCGGGGCATCCCCTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTTCGGGGCATCCCCTTGC'})
({'score': 10, 'version': 0L, 'sequence': 'CAGCCAGAAACTGACTTATC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGCCAGAAACTGACTTATC'})
({'score': 6, 'version': 0L, 'sequence': 'CAGCTGCAGATAGCACAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGCTGCAGATAGCACAGTC'})
({'score': 17, 'version': 0L, 'sequence': 'CTGCAACTCCGCTCATACTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGCAACTCCGCTCATACTC'})
({'score': 33, 'version': 0L, 'sequence': 'TGCAGGTCACCGGTTAAACC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCAGGTCACCGGTTAAACC'})
({'score': 30, 'version': 0L, 'sequence': 'TCCTCTAAAGTTTTGATGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCTCTAAAGTTTTGATGCC'})
({'score': 5, 'version': 0L, 'sequence': 'TATTGCAAGATGGGCACAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TATTGCAAGATGGGCACAGC'})
({'score': 27, 'version': 0L, 'sequence': 'GATAGAGCTTCATATGAACC'}, {'score': 100, 'version': 2L, 'sequence': 'GATAGAGCTTCATATGAACC'})
({'score': 23, 'version': 0L, 'sequence': 'ATCCACCAGTGGGCATAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCCACCAGTGGGCATAAAC'})
({'score': 39, 'version': 0L, 'sequence': 'AAATTGAAATTTGGTAGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATTGAAATTTGGTAGAGC'})
({'score': 7, 'version': 0L, 'sequence': 'ACCTTCCTCCGGAGGAGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCTTCCTCCGGAGGAGGCC'})
({'score': 23, 'version': 0L, 'sequence': 'TTCCCCAATGCTGTTTGCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCCCCAATGCTGTTTGCCC'})
({'score': 36, 'version': 0L, 'sequence': 'TCAGTAAGCCTGCCGTTGAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCAGTAAGCCTGCCGTTGAC'})
({'score': 60, 'version': 0L, 'sequence': 'AAGCCCACGTGCCAAGATTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGCCCACGTGCCAAGATTC'})
({'score': 27, 'version': 0L, 'sequence': 'CTGAATCCAGTTTCTGTATC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGAATCCAGTTTCTGTATC'})
({'score': 49, 'version': 0L, 'sequence': 'TGGATCAGCTCTGGTATTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGATCAGCTCTGGTATTAC'})
({'score': 37, 'version': 0L, 'sequence': 'ATTAGCAAATAGACTCAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATTAGCAAATAGACTCAAAC'})
({'score': 40, 'version': 0L, 'sequence': 'AGTCCCTGCACTGAGTTATC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTCCCTGCACTGAGTTATC'})
({'score': 49, 'version': 0L, 'sequence': 'ACTGACTTACCAAGTGCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTGACTTACCAAGTGCCCC'})
({'score': 27, 'version': 0L, 'sequence': 'CACACTACGTATTACAGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CACACTACGTATTACAGTTC'})
({'score': 49, 'version': 0L, 'sequence': 'CTGGCTAAGGGAGATGTGCC'}, {'score': 37, 'version': 2L, 'sequence': 'CTGGCTAAGGGAGATGTGCC'})
({'score': 28, 'version': 0L, 'sequence': 'CTCTGTGCTGTTTGCATACC'}, {'score': 100, 'version': 2L, 'sequence': 'CTCTGTGCTGTTTGCATACC'})
({'score': 40, 'version': 0L, 'sequence': 'ACAATCAGACGAGACCGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'ACAATCAGACGAGACCGAGC'})
({'score': 40, 'version': 0L, 'sequence': 'TCGGTCTCGTCTGATTGTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCGGTCTCGTCTGATTGTAC'})
({'score': 39, 'version': 0L, 'sequence': 'GGCAGAGGCAGATTTATCTC'}, {'score': 32, 'version': 2L, 'sequence': 'GGCAGAGGCAGATTTATCTC'})
({'score': 50, 'version': 0L, 'sequence': 'GCAGGGAGTCTCCAATGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCAGGGAGTCTCCAATGGCC'})
({'score': 50, 'version': 0L, 'sequence': 'AAGGTCCAATAGCAACCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGGTCCAATAGCAACCCTC'})
({'score': 50, 'version': 0L, 'sequence': 'ACAGAAATTGAGTTCTTGAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACAGAAATTGAGTTCTTGAC'})
({'score': 33, 'version': 0L, 'sequence': 'TGCCCAGTTAGCAAATGTTC'}, {'score': 21, 'version': 2L, 'sequence': 'TGCCCAGTTAGCAAATGTTC'})
({'score': 33, 'version': 0L, 'sequence': 'CACCCCTCTACCCATGACAC'}, {'score': 100, 'version': 2L, 'sequence': 'CACCCCTCTACCCATGACAC'})
({'score': 49, 'version': 0L, 'sequence': 'TCCATACCTGGGTCCATGAC'}, {'score': 40, 'version': 2L, 'sequence': 'TCCATACCTGGGTCCATGAC'})
({'score': 60, 'version': 0L, 'sequence': 'CTGGATGGGAGATTTTAACC'}, {'score': 37, 'version': 2L, 'sequence': 'CTGGATGGGAGATTTTAACC'})
({'score': 65, 'version': 0L, 'sequence': 'CAGAGTGTGGGAAAGTTTCC'}, {'score': 64, 'version': 2L, 'sequence': 'CAGAGTGTGGGAAAGTTTCC'})
({'score': 97, 'version': 0L, 'sequence': 'CCGGACTTTCGAGCAGTGTC'}, {'score': 100, 'version': 2L, 'sequence': 'CCGGACTTTCGAGCAGTGTC'})
({'score': 52, 'version': 0L, 'sequence': 'GGTTAAACGCTCCATGGTAC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTTAAACGCTCCATGGTAC'})
({'score': 96, 'version': 0L, 'sequence': 'GTCTACCAAGGTGCCCTGTC'}, {'score': 92, 'version': 2L, 'sequence': 'GTCTACCAAGGTGCCCTGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CACAAATACAAATATGCAGC'}, {'score': 69, 'version': 2L, 'sequence': 'CACAAATACAAATATGCAGC'})
({'score': 46, 'version': 0L, 'sequence': 'ACTTTCTCCTTGTTTGGCCC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTTTCTCCTTGTTTGGCCC'})
({'score': 91, 'version': 0L, 'sequence': 'TGGAGAGCAATAAAGGGTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGAGAGCAATAAAGGGTAC'})
({'score': 51, 'version': 0L, 'sequence': 'CCTGGTTGCTTGTGTGGCCC'}, {'score': 52, 'version': 2L, 'sequence': 'CCTGGTTGCTTGTGTGGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTAAACACACCATCGATTC'}, {'score': 74, 'version': 2L, 'sequence': 'ACTAAACACACCATCGATTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTGTCTCTTCACTGGTTGC'}, {'score': 55, 'version': 2L, 'sequence': 'GCTGTCTCTTCACTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'ACAGGCTGAGGTCCGGTACC'}, {'score': 46, 'version': 2L, 'sequence': 'ACAGGCTGAGGTCCGGTACC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTGTGTTTTAGTGACAGC'}, {'score': 69, 'version': 2L, 'sequence': 'TTCTGTGTTTTAGTGACAGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTTACCTTTTAGTATGATC'}, {'score': 16, 'version': 2L, 'sequence': 'ATTTACCTTTTAGTATGATC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCAGCAGCTATTTGTTTGC'}, {'score': 23, 'version': 2L, 'sequence': 'TGCAGCAGCTATTTGTTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTAAGGGTAATTTGGACCC'}, {'score': 19, 'version': 2L, 'sequence': 'GCTAAGGGTAATTTGGACCC'})
({'score': 97, 'version': 0L, 'sequence': 'GACCCTGTACTGGGGAGAAC'}, {'score': 100, 'version': 2L, 'sequence': 'GACCCTGTACTGGGGAGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCTTTAACCTGCAGCCCTC'}, {'score': 93, 'version': 2L, 'sequence': 'CCCTTTAACCTGCAGCCCTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTGTAGTTCAGCAATACC'}, {'score': 62, 'version': 2L, 'sequence': 'TGCTGTAGTTCAGCAATACC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCTAAAAAAACAAAGTTGC'}, {'score': 97, 'version': 2L, 'sequence': 'CCCTAAAAAAACAAAGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTGCAGCTATCTGGTTGC'}, {'score': 11, 'version': 2L, 'sequence': 'TGCTGCAGCTATCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CATCTCCAGAGGCTCCTTCC'}, {'score': 98, 'version': 2L, 'sequence': 'CATCTCCAGAGGCTCCTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGGAAATGAACAATATGAC'}, {'score': 1, 'version': 2L, 'sequence': 'TGGGAAATGAACAATATGAC'})
({'score': 87, 'version': 0L, 'sequence': 'TCACCCAGGGACTGCAGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'TCACCCAGGGACTGCAGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCCATAATTGCTCTGTAGC'}, {'score': 52, 'version': 2L, 'sequence': 'TTCCATAATTGCTCTGTAGC'})
({'score': 90, 'version': 0L, 'sequence': 'TTCTACAGAGCCTTTGGCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCTACAGAGCCTTTGGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTAAACAAGACATTAAGC'}, {'score': 99, 'version': 2L, 'sequence': 'GTGTAAACAAGACATTAAGC'})
({'score': 89, 'version': 0L, 'sequence': 'AAATTTGAATGAAGGAGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATTTGAATGAAGGAGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGAAAAATGAATATGAAAC'}, {'score': 96, 'version': 2L, 'sequence': 'CTGAAAAATGAATATGAAAC'})
({'score': 46, 'version': 0L, 'sequence': 'TTTGTGTTTTTTGTTAGTTC'}, {'score': 96, 'version': 2L, 'sequence': 'TTTGTGTTTTTTGTTAGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTTAAACTCTCAGATGACC'}, {'score': 99, 'version': 2L, 'sequence': 'GGTTAAACTCTCAGATGACC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTACATCAAAAGCGTTCAC'}, {'score': 96, 'version': 2L, 'sequence': 'CTTACATCAAAAGCGTTCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GATGTGGTCCCCAATCCCAC'}, {'score': 40, 'version': 2L, 'sequence': 'GATGTGGTCCCCAATCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGCTGACAGAGCTACAATC'}, {'score': 93, 'version': 2L, 'sequence': 'GAGCTGACAGAGCTACAATC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTCACCTAACATTTTGTC'}, {'score': 97, 'version': 2L, 'sequence': 'GTGTCACCTAACATTTTGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCCATTCACAGGCCGATTC'}, {'score': 3, 'version': 2L, 'sequence': 'GCCCATTCACAGGCCGATTC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGGATTGATATTGGCAGC'}, {'score': 17, 'version': 2L, 'sequence': 'AAAGGATTGATATTGGCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTATTAGCTACCTGGTTGC'}, {'score': 12, 'version': 2L, 'sequence': 'TTTATTAGCTACCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTAAGTGCCACTGGGTTGC'}, {'score': 99, 'version': 2L, 'sequence': 'TGTAAGTGCCACTGGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GACAAGTGAGTGAGACAGCC'}, {'score': 29, 'version': 2L, 'sequence': 'GACAAGTGAGTGAGACAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTGCAACATTCAGTCATTC'}, {'score': 91, 'version': 2L, 'sequence': 'ACTGCAACATTCAGTCATTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTGCTTTCCCAGCTGCCC'}, {'score': 94, 'version': 2L, 'sequence': 'TGCTGCTTTCCCAGCTGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GACTTAAAGGGCACTGTCAC'}, {'score': 90, 'version': 2L, 'sequence': 'GACTTAAAGGGCACTGTCAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGCAAGGAAAAGGTATGGC'}, {'score': 98, 'version': 2L, 'sequence': 'AGGCAAGGAAAAGGTATGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTTGCAGAAATACAACTCC'}, {'score': 9, 'version': 2L, 'sequence': 'TGTTGCAGAAATACAACTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTATTTTATCATTCAAAC'}, {'score': 92, 'version': 2L, 'sequence': 'TTTTATTTTATCATTCAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAGTTAGGGTCAGGGACCC'}, {'score': 90, 'version': 2L, 'sequence': 'GTAGTTAGGGTCAGGGACCC'})
({'score': 85, 'version': 0L, 'sequence': 'CAACTGGGCAGAAGCAGCAC'}, {'score': 87, 'version': 2L, 'sequence': 'CAACTGGGCAGAAGCAGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTGGCACAATAGGTTTTAGC'}, {'score': 99, 'version': 2L, 'sequence': 'TTGGCACAATAGGTTTTAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCCTGTTCTAAACACAGCC'}, {'score': 70, 'version': 2L, 'sequence': 'TCCCTGTTCTAAACACAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATACACAATACACACAATAC'}, {'score': 66, 'version': 2L, 'sequence': 'ATACACAATACACACAATAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAAATGTAATAAAGAAATC'}, {'score': 46, 'version': 2L, 'sequence': 'TCAAATGTAATAAAGAAATC'})
({'score': 100, 'version': 0L, 'sequence': 'GCATTCTTTTTTTTTCTGTC'}, {'score': 95, 'version': 2L, 'sequence': 'GCATTCTTTTTTTTTCTGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAGCTTTTCACCAAAAATC'}, {'score': 95, 'version': 2L, 'sequence': 'GTAGCTTTTCACCAAAAATC'})
({'score': 94, 'version': 0L, 'sequence': 'GAACAAGAACTCTGTGGATC'}, {'score': 100, 'version': 2L, 'sequence': 'GAACAAGAACTCTGTGGATC'})
({'score': 55, 'version': 0L, 'sequence': 'CTAAAGTTTAGTGGAGGGTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTAAAGTTTAGTGGAGGGTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTACACAGGTGGAAGCTTAC'}, {'score': 98, 'version': 2L, 'sequence': 'TTACACAGGTGGAAGCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTACCCCAGGCTGATGTGC'}, {'score': 99, 'version': 2L, 'sequence': 'ACTACCCCAGGCTGATGTGC'})
({'score': 98, 'version': 0L, 'sequence': 'GCTAAGGACAATCTAAGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTAAGGACAATCTAAGCAC'})
({'score': 49, 'version': 0L, 'sequence': 'TTCTCCTGTGGTTTCAGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCTCCTGTGGTTTCAGCTC'})
({'score': 62, 'version': 0L, 'sequence': 'AACTTTAGAAGCTGAAGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AACTTTAGAAGCTGAAGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'ACCTGTATATCTTCTATTTC'}, {'score': 67, 'version': 2L, 'sequence': 'ACCTGTATATCTTCTATTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGTGTAGAGTGTCTGTGTC'}, {'score': 74, 'version': 2L, 'sequence': 'CTGTGTAGAGTGTCTGTGTC'})
({'score': 98, 'version': 0L, 'sequence': 'GAATATACAAATGGAGGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GAATATACAAATGGAGGGCC'})
({'score': 82, 'version': 0L, 'sequence': 'CGCAGGAGGCGGGGCAGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'CGCAGGAGGCGGGGCAGAGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGCCACAGAAAGTAATGAC'}, {'score': 98, 'version': 2L, 'sequence': 'GGGCCACAGAAAGTAATGAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAACGTTTCAGTCCTTTAC'}, {'score': 5, 'version': 2L, 'sequence': 'CCAACGTTTCAGTCCTTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCAGCACCTATTTGGTTGC'}, {'score': 0, 'version': 2L, 'sequence': 'TTCAGCACCTATTTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGCAACAAAGGCCCAGGCC'}, {'score': 0, 'version': 2L, 'sequence': 'AGGCAACAAAGGCCCAGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGAACATGTAAGTCGC'}, {'score': 93, 'version': 2L, 'sequence': 'GCGAACATGTAAGTCGC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTCTGTTATAAAGATAGC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCTCTGTTATAAAGATAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCCATACACGGGCCAATTC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCCATACACGGGCCAATTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTAAGGTAATTTAGATCC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCTAAGGTAATTTAGATCC'})
({'score': 95, 'version': 0L, 'sequence': 'GATGTATAAGTTTCAGTGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GATGTATAAGTTTCAGTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCATTATAAATGGCTGCCCC'}, {'score': 62, 'version': 2L, 'sequence': 'TCATTATAAATGGCTGCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCATTATAAATGGCTGCCCC'}, {'score': 62, 'version': 2L, 'sequence': 'TCATTATAAATGGCTGCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AATCCCCTGCTGTGTAGCCC'}, {'score': 18, 'version': 2L, 'sequence': 'AATCCCCTGCTGTGTAGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AATCCCCTGCTGTGTAGCCC'}, {'score': 18, 'version': 2L, 'sequence': 'AATCCCCTGCTGTGTAGCCC'})
({'score': 99, 'version': 0L, 'sequence': 'ATTCCAGGCATTTTGTCTCC'}, {'score': 72, 'version': 2L, 'sequence': 'ATTCCAGGCATTTTGTCTCC'})
({'score': 99, 'version': 0L, 'sequence': 'TATCTTATTTCCATCATCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TATCTTATTTCCATCATCTC'})
({'score': 99, 'version': 0L, 'sequence': 'GCCAAGGAGTGGATCTTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCAAGGAGTGGATCTTCTC'})
({'score': 100, 'version': 0L, 'sequence': 'ACCCATAGGTGGGGGATATC'}, {'score': 0, 'version': 2L, 'sequence': 'ACCCATAGGTGGGGGATATC'})
({'score': 98, 'version': 0L, 'sequence': 'AAGGGCTGCTGGGTATCAGC'}, {'score': 99, 'version': 2L, 'sequence': 'AAGGGCTGCTGGGTATCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACATTCTGTTCTCTTACC'}, {'score': 96, 'version': 2L, 'sequence': 'TCACATTCTGTTCTCTTACC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACATTCTGTTCTCTTACC'}, {'score': 96, 'version': 2L, 'sequence': 'TCACATTCTGTTCTCTTACC'})
({'score': 100, 'version': 0L, 'sequence': 'AGATCTGAGTGTATGGCCAC'}, {'score': 95, 'version': 2L, 'sequence': 'AGATCTGAGTGTATGGCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCGCAGGTGACTAATCTCCC'}, {'score': 1, 'version': 2L, 'sequence': 'TCGCAGGTGACTAATCTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCGCAGGTGACTAATCTCCC'}, {'score': 1, 'version': 2L, 'sequence': 'TCGCAGGTGACTAATCTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTCTAGCAGTAGGTTTATC'}, {'score': 79, 'version': 2L, 'sequence': 'AGTCTAGCAGTAGGTTTATC'})
({'score': 96, 'version': 0L, 'sequence': 'GGAGGGAACACTGTAGGAGC'}, {'score': 99, 'version': 2L, 'sequence': 'GGAGGGAACACTGTAGGAGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCATAGATGTCTCCCCC'}, {'score': 28, 'version': 2L, 'sequence': 'AGTGCATAGATGTCTCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCATAGATGTCTCCCCC'}, {'score': 28, 'version': 2L, 'sequence': 'AGTGCATAGATGTCTCCCCC'})
({'score': 88, 'version': 0L, 'sequence': 'TAAATAAATGTAAGTCAGGC'}, {'score': 99, 'version': 2L, 'sequence': 'TAAATAAATGTAAGTCAGGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGGGCTGTGAGTTTGAATC'}, {'score': 59, 'version': 2L, 'sequence': 'AGGGGCTGTGAGTTTGAATC'})
({'score': 98, 'version': 0L, 'sequence': 'TATATACAGTGTTCCAGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'TATATACAGTGTTCCAGGGC'})
({'score': 97, 'version': 0L, 'sequence': 'GAATCCATAGATTGCCAGTC'}, {'score': 0, 'version': 2L, 'sequence': 'GAATCCATAGATTGCCAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCATAGATGTCTCCCCC'}, {'score': 28, 'version': 2L, 'sequence': 'AGTGCATAGATGTCTCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCATAGATGTCTCCCCC'}, {'score': 28, 'version': 2L, 'sequence': 'AGTGCATAGATGTCTCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTCCTATGATGTCATCAC'}, {'score': 40, 'version': 2L, 'sequence': 'CTCTCCTATGATGTCATCAC'})
({'score': 99, 'version': 0L, 'sequence': 'GGAGCTGGGCCAGCCAGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGAGCTGGGCCAGCCAGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGCAATAGGCTTTGGCTGC'}, {'score': 0, 'version': 2L, 'sequence': 'GAGCAATAGGCTTTGGCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTAGGTTTTCTCTGACCC'}, {'score': 66, 'version': 2L, 'sequence': 'TTCTAGGTTTTCTCTGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTAGGTTTTCTCTGACCC'}, {'score': 66, 'version': 2L, 'sequence': 'TTCTAGGTTTTCTCTGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCATAGATGTCTCCCCC'}, {'score': 28, 'version': 2L, 'sequence': 'AGTGCATAGATGTCTCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCATAGATGTCTCCCCC'}, {'score': 28, 'version': 2L, 'sequence': 'AGTGCATAGATGTCTCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATGTGTATCGCTGCGGCGCC'}, {'score': 19, 'version': 2L, 'sequence': 'ATGTGTATCGCTGCGGCGCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATGTGTATCGCTGCGGCGCC'}, {'score': 19, 'version': 2L, 'sequence': 'ATGTGTATCGCTGCGGCGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTCCTGTGATGTCATCAC'}, {'score': 32, 'version': 2L, 'sequence': 'CTCTCCTGTGATGTCATCAC'})
({'score': 95, 'version': 0L, 'sequence': 'TCCCCATACATGGGCAGATC'}, {'score': 20, 'version': 2L, 'sequence': 'TCCCCATACATGGGCAGATC'})
({'score': 99, 'version': 0L, 'sequence': 'GGGCATAGCAAAAGTCGGTC'}, {'score': 1, 'version': 2L, 'sequence': 'GGGCATAGCAAAAGTCGGTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTTCCAAATTGGCCGCCCC'}, {'score': 1, 'version': 2L, 'sequence': 'ATTTCCAAATTGGCCGCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTTCCAAATTGGCCGCCCC'}, {'score': 1, 'version': 2L, 'sequence': 'ATTTCCAAATTGGCCGCCCC'})
({'score': 99, 'version': 0L, 'sequence': 'ATATGAAATATGGGCCAGGC'}, {'score': 100, 'version': 2L, 'sequence': 'ATATGAAATATGGGCCAGGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACTGACC'}, {'score': 22, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACTGACC'}, {'score': 22, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGGGGCGCC'}, {'score': 16, 'version': 2L, 'sequence': 'ACACACAGGAGAGGGGCGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGAGGCGCC'}, {'score': 28, 'version': 2L, 'sequence': 'ACACACAGGAGAGAGGCGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACTGACC'}, {'score': 22, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACTGACC'}, {'score': 22, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGAGGCGCC'}, {'score': 28, 'version': 2L, 'sequence': 'ACACACAGGAGAGAGGCGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGAGGCGCC'}, {'score': 28, 'version': 2L, 'sequence': 'ACACACAGGAGAGAGGCGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACTGACC'}, {'score': 22, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACTGACC'}, {'score': 22, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGAGGCGCC'}, {'score': 28, 'version': 2L, 'sequence': 'ACACACAGGAGAGAGGCGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACTGACC'}, {'score': 22, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACTGACC'}, {'score': 22, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGGGGCGCC'}, {'score': 16, 'version': 2L, 'sequence': 'ACACACAGGAGAGGGGCGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGGGGCGCC'}, {'score': 16, 'version': 2L, 'sequence': 'ACACACAGGAGAGGGGCGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGGGGCGCC'}, {'score': 16, 'version': 2L, 'sequence': 'ACACACAGGAGAGGGGCGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGGGGCGCC'}, {'score': 16, 'version': 2L, 'sequence': 'ACACACAGGAGAGGGGCGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGGGGCACC'}, {'score': 99, 'version': 2L, 'sequence': 'ACACACAGGAGAGGGGCACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAAGAGAGGGGCGCC'}, {'score': 12, 'version': 2L, 'sequence': 'ACACACAAGAGAGGGGCGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGGGGCGCC'}, {'score': 16, 'version': 2L, 'sequence': 'ACACACAGGAGAGGGGCGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGTGTGGCACCGACAGACC'}, {'score': 14, 'version': 2L, 'sequence': 'GTGTGTGGCACCGACAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACACAGGAGAGGGGCGCC'}, {'score': 16, 'version': 2L, 'sequence': 'ACACACAGGAGAGGGGCGCC'})
({'score': 99, 'version': 0L, 'sequence': 'ACACACAGGAGGGGGGCGCC'}, {'score': 22, 'version': 2L, 'sequence': 'ACACACAGGAGGGGGGCGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGTGTGGGTGGCCCAGCTC'}, {'score': 95, 'version': 2L, 'sequence': 'TAGTGTGGGTGGCCCAGCTC'})
({'score': 97, 'version': 0L, 'sequence': 'GCCATCTCTGGTATGTGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCATCTCTGGTATGTGGGC'})
({'score': 96, 'version': 0L, 'sequence': 'ACCTTTTGTATCTGGGCAGC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCTTTTGTATCTGGGCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTACATGCAGTAGCTACGC'}, {'score': 99, 'version': 2L, 'sequence': 'TTTACATGCAGTAGCTACGC'})
({'score': 100, 'version': 0L, 'sequence': 'CAATGCAGCGAGGTGCTGCC'}, {'score': 93, 'version': 2L, 'sequence': 'CAATGCAGCGAGGTGCTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CAATGCAGCGAGGTGCTGCC'}, {'score': 93, 'version': 2L, 'sequence': 'CAATGCAGCGAGGTGCTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTAATGATGTGTAAGTGCC'}, {'score': 99, 'version': 2L, 'sequence': 'TTTAATGATGTGTAAGTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CGGCTCGTTGATACCGTCCC'}, {'score': 10, 'version': 2L, 'sequence': 'CGGCTCGTTGATACCGTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CGGCTCGTTGATACCGTCCC'}, {'score': 10, 'version': 2L, 'sequence': 'CGGCTCGTTGATACCGTCCC'})
({'score': 96, 'version': 0L, 'sequence': 'TATGCCCCTTAGTGAAGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'TATGCCCCTTAGTGAAGGGC'})
({'score': 55, 'version': 0L, 'sequence': 'CCTTCCTTGTCTTCTGCAGC'}, {'score': 62, 'version': 2L, 'sequence': 'CCTTCCTTGTCTTCTGCAGC'})
({'score': 58, 'version': 0L, 'sequence': 'ATTGGGAAAGGGGAAGGAGC'}, {'score': 62, 'version': 2L, 'sequence': 'ATTGGGAAAGGGGAAGGAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTTTTATGAACTGGATGC'}, {'score': 95, 'version': 2L, 'sequence': 'TTTTTTTATGAACTGGATGC'})
({'score': 93, 'version': 0L, 'sequence': 'AGAAGATTAAAAACCTCAGC'}, {'score': 99, 'version': 2L, 'sequence': 'AGAAGATTAAAAACCTCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTCATCCTCTTTGTTCTCC'}, {'score': 96, 'version': 2L, 'sequence': 'CTTCATCCTCTTTGTTCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTCCTTGGCTGGGGCCCAC'}, {'score': 5, 'version': 2L, 'sequence': 'TGTCCTTGGCTGGGGCCCAC'})
({'score': 99, 'version': 0L, 'sequence': 'GCTGGTTGGGTGAGCAGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTGGTTGGGTGAGCAGGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GCTGGTTGGGTGAGCAGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTGGTTGGGTGAGCAGGCC'})
({'score': 96, 'version': 0L, 'sequence': 'GCCTCCCTGTAATTGATGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCTCCCTGTAATTGATGGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCAGGTTACACACAGGGTGC'}, {'score': 99, 'version': 2L, 'sequence': 'GCAGGTTACACACAGGGTGC'})
({'score': 89, 'version': 0L, 'sequence': 'CGGAAGTTCTGTGAGAAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'CGGAAGTTCTGTGAGAAAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTAATGTGTTGTTTATTGC'}, {'score': 99, 'version': 2L, 'sequence': 'CCTAATGTGTTGTTTATTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CGCACAGGCCTTCTGAAAAC'}, {'score': 91, 'version': 2L, 'sequence': 'CGCACAGGCCTTCTGAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGAACCTACCCAGGAGTGGC'}, {'score': 0, 'version': 2L, 'sequence': 'TGAACCTACCCAGGAGTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'GAATTCTGAAGGAGAATGTC'}, {'score': 2, 'version': 2L, 'sequence': 'GAATTCTGAAGGAGAATGTC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 96, 'version': 0L, 'sequence': 'AAGCAGGCAGATTCCCAGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGCAGGCAGATTCCCAGCC'})
({'score': 96, 'version': 0L, 'sequence': 'AAGCAGGCAGATTCCCAGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGCAGGCAGATTCCCAGCC'})
({'score': 99, 'version': 0L, 'sequence': 'CCTGCTCTGGGCTTCTGTGC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTGCTCTGGGCTTCTGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTCCAGTTTGGGGAATAAC'}, {'score': 99, 'version': 2L, 'sequence': 'AGTCCAGTTTGGGGAATAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CGGCTAGAATGTAAACCGC'}, {'score': 99, 'version': 2L, 'sequence': 'CGGCTAGAATGTAAACCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGGTTTACATTCTAGC'}, {'score': 98, 'version': 2L, 'sequence': 'GCGGTTTACATTCTAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTGCCTTGTGGGTGCCAC'}, {'score': 97, 'version': 2L, 'sequence': 'CTCTGCCTTGTGGGTGCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCTCAAGTTTTGTGACTGC'}, {'score': 66, 'version': 2L, 'sequence': 'AGCTCAAGTTTTGTGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCTCAAGTTTTGTGACTGC'}, {'score': 66, 'version': 2L, 'sequence': 'AGCTCAAGTTTTGTGACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGATAGGGTTGCCACCTGGC'}, {'score': 2, 'version': 2L, 'sequence': 'AGATAGGGTTGCCACCTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CGACTGGGTCAGTGAAATAC'}, {'score': 95, 'version': 2L, 'sequence': 'CGACTGGGTCAGTGAAATAC'})
({'score': 97, 'version': 0L, 'sequence': 'GGAAATATATCAGCAAGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGAAATATATCAGCAAGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAATTTGTAATACCGCTCC'}, {'score': 78, 'version': 2L, 'sequence': 'TAAATTTGTAATACCGCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAATTTGTAATACCGCTCC'}, {'score': 78, 'version': 2L, 'sequence': 'TAAATTTGTAATACCGCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGCGGTATTACAAATTTAC'}, {'score': 25, 'version': 2L, 'sequence': 'GAGCGGTATTACAAATTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AATTGATGTATTTATGTTTC'}, {'score': 92, 'version': 2L, 'sequence': 'AATTGATGTATTTATGTTTC'})
({'score': 0, 'version': 0L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCAATTGTTTTTTGGCTGC'}, {'score': 0, 'version': 2L, 'sequence': 'GGCAATTGTTTTTTGGCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGGACACACTTATGGGCTC'}, {'score': 98, 'version': 2L, 'sequence': 'CTGGACACACTTATGGGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGGGGGGTGAAGAGCCCAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTGGGGGGTGAAGAGCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCCCCTGGGTTGGGAGCCC'}, {'score': 0, 'version': 2L, 'sequence': 'GGCCCCTGGGTTGGGAGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTGGGCCTCAGGGGCCCAC'}, {'score': 2, 'version': 2L, 'sequence': 'TCTGGGCCTCAGGGGCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCACCTTGTGTTACTGCCCC'}, {'score': 50, 'version': 2L, 'sequence': 'CCACCTTGTGTTACTGCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCACCTTGTGTTACTGCCCC'}, {'score': 50, 'version': 2L, 'sequence': 'CCACCTTGTGTTACTGCCCC'})
({'score': 99, 'version': 0L, 'sequence': 'GGGGTAAGTGGGGGGAGCAC'}, {'score': 92, 'version': 2L, 'sequence': 'GGGGTAAGTGGGGGGAGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTTTTTCCTGGTATCTCGC'}, {'score': 80, 'version': 2L, 'sequence': 'GTTTTTTCCTGGTATCTCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCAAATTACCCTAGCAAC'}, {'score': 1, 'version': 2L, 'sequence': 'GCTCAAATTACCCTAGCAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTCTCATACAAACACTGCC'}, {'score': 89, 'version': 2L, 'sequence': 'CCTCTCATACAAACACTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTCTCATACAAACACTGCC'}, {'score': 89, 'version': 2L, 'sequence': 'CCTCTCATACAAACACTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTAAGATAATTAGCACCC'}, {'score': 14, 'version': 2L, 'sequence': 'TGCTAAGATAATTAGCACCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTAAGATAATTAGCACCC'}, {'score': 14, 'version': 2L, 'sequence': 'TGCTAAGATAATTAGCACCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCCTGAAATTCGATCCCAC'}, {'score': 49, 'version': 2L, 'sequence': 'TTCCTGAAATTCGATCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTGCGATGTACATTCTCGC'}, {'score': 33, 'version': 2L, 'sequence': 'CCTGCGATGTACATTCTCGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCCCGAAATTCGATCCCAC'}, {'score': 27, 'version': 2L, 'sequence': 'TTCCCGAAATTCGATCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGAGATCTGCCAGATTTC'}, {'score': 4, 'version': 2L, 'sequence': 'ATTGAGATCTGCCAGATTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTACCCGACTGATATCTGTC'}, {'score': 81, 'version': 2L, 'sequence': 'CTACCCGACTGATATCTGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAACGATTTACATTCTAGC'}, {'score': 22, 'version': 2L, 'sequence': 'CTAACGATTTACATTCTAGC'})
({'score': 100, 'version': 0L, 'sequence': 'ACGTTGTAGAAACCACTGGC'}, {'score': 98, 'version': 2L, 'sequence': 'ACGTTGTAGAAACCACTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CATGGGCAGATTTAAGTTGC'}, {'score': 24, 'version': 2L, 'sequence': 'CATGGGCAGATTTAAGTTGC'})
({'score': 99, 'version': 0L, 'sequence': 'TTGTTCTGAAGGACCCGAAC'}, {'score': 33, 'version': 2L, 'sequence': 'TTGTTCTGAAGGACCCGAAC'})
({'score': 99, 'version': 0L, 'sequence': 'GGCCCTGATTGGTTGCGCCC'}, {'score': 45, 'version': 2L, 'sequence': 'GGCCCTGATTGGTTGCGCCC'})
({'score': 99, 'version': 0L, 'sequence': 'GGCCCTGATTGGTTGCGCCC'}, {'score': 45, 'version': 2L, 'sequence': 'GGCCCTGATTGGTTGCGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CACTGCATCAGAACACGCAC'}, {'score': 50, 'version': 2L, 'sequence': 'CACTGCATCAGAACACGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTCTGATGCAGTGGGGAGC'}, {'score': 14, 'version': 2L, 'sequence': 'GTTCTGATGCAGTGGGGAGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGAGAGATAATCTGCAGCC'}, {'score': 99, 'version': 2L, 'sequence': 'GGGAGAGATAATCTGCAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGAGAGATAATCTGCAGCC'}, {'score': 99, 'version': 2L, 'sequence': 'GGGAGAGATAATCTGCAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAGCAACAGGGGAATAAGC'}, {'score': 93, 'version': 2L, 'sequence': 'TCAGCAACAGGGGAATAAGC'})
({'score': 100, 'version': 0L, 'sequence': 'ACCAGGTACGTGGGCACATC'}, {'score': 95, 'version': 2L, 'sequence': 'ACCAGGTACGTGGGCACATC'})
({'score': 100, 'version': 0L, 'sequence': 'TATATATGCAATATCACCCC'}, {'score': 94, 'version': 2L, 'sequence': 'TATATATGCAATATCACCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TATATATGCAATATCACCCC'}, {'score': 94, 'version': 2L, 'sequence': 'TATATATGCAATATCACCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTGCCCAGTTTGTGCCAC'}, {'score': 99, 'version': 2L, 'sequence': 'TTCTGCCCAGTTTGTGCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCTGTGATGTGTGTCCTGC'}, {'score': 95, 'version': 2L, 'sequence': 'CCCTGTGATGTGTGTCCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGCTGGCGGATAGAGTGGC'}, {'score': 93, 'version': 2L, 'sequence': 'GAGCTGGCGGATAGAGTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCAATAGTTCTTTTGCTGC'}, {'score': 2, 'version': 2L, 'sequence': 'AGCAATAGTTCTTTTGCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTACTAGGAATTACATTAC'}, {'score': 98, 'version': 2L, 'sequence': 'TTTACTAGGAATTACATTAC'})
({'score': 100, 'version': 0L, 'sequence': 'CACCATCTGTATTGTGCCAC'}, {'score': 88, 'version': 2L, 'sequence': 'CACCATCTGTATTGTGCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CGCACACAGGACCTGTGCCC'}, {'score': 96, 'version': 2L, 'sequence': 'CGCACACAGGACCTGTGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CGCACACAGGACCTGTGCCC'}, {'score': 96, 'version': 2L, 'sequence': 'CGCACACAGGACCTGTGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCAGAGGGAGGAGAAGAAGC'}, {'score': 98, 'version': 2L, 'sequence': 'GCAGAGGGAGGAGAAGAAGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTCTCATTACTCCCTGTTC'}, {'score': 95, 'version': 2L, 'sequence': 'ATTCTCATTACTCCCTGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CATTAGATTGTAAGCTCTGC'}, {'score': 1, 'version': 2L, 'sequence': 'CATTAGATTGTAAGCTCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTACCTCCTGCTATTCAACC'}, {'score': 97, 'version': 2L, 'sequence': 'GTACCTCCTGCTATTCAACC'})
({'score': 100, 'version': 0L, 'sequence': 'GTACCTCCTGCTATTCAACC'}, {'score': 97, 'version': 2L, 'sequence': 'GTACCTCCTGCTATTCAACC'})
({'score': 100, 'version': 0L, 'sequence': 'CGTCTGTGGGCCCCATACAC'}, {'score': 10, 'version': 2L, 'sequence': 'CGTCTGTGGGCCCCATACAC'})
({'score': 95, 'version': 0L, 'sequence': 'AAAAGTTACAGCCAGCAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAAGTTACAGCCAGCAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTCTATTCATGAATTAAAC'}, {'score': 99, 'version': 2L, 'sequence': 'TTTCTATTCATGAATTAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTTTGGGGGTGACCATATC'}, {'score': 97, 'version': 2L, 'sequence': 'AGTTTGGGGGTGACCATATC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTGTCAGATGCTGTTTTCC'}, {'score': 94, 'version': 2L, 'sequence': 'CCTGTCAGATGCTGTTTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTGTCAGATGCTGTTTTCC'}, {'score': 94, 'version': 2L, 'sequence': 'CCTGTCAGATGCTGTTTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'CAAGGAGTGCAATGTGGTTC'}, {'score': 99, 'version': 2L, 'sequence': 'CAAGGAGTGCAATGTGGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAGTGGTAGCATGGGGTTC'}, {'score': 95, 'version': 2L, 'sequence': 'CTAGTGGTAGCATGGGGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTATAACTGATGACATCAC'}, {'score': 34, 'version': 2L, 'sequence': 'ATTATAACTGATGACATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCAGGTGGTGAGCAAAATC'}, {'score': 97, 'version': 2L, 'sequence': 'AGCAGGTGGTGAGCAAAATC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTCTGTTTTATTTACATGC'}, {'score': 0, 'version': 2L, 'sequence': 'AGTCTGTTTTATTTACATGC'})
({'score': 99, 'version': 0L, 'sequence': 'GCATGTAAATAAAACAGAC'}, {'score': 7, 'version': 2L, 'sequence': 'GCATGTAAATAAAACAGAC'})
({'score': 6, 'version': 0L, 'sequence': 'CGGGGAGATTAGTCGCCCGC'}, {'score': 15, 'version': 2L, 'sequence': 'CGGGGAGATTAGTCGCCCGC'})
({'score': 0, 'version': 0L, 'sequence': 'TCCTGGATTCAGTGCATCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCTGGATTCAGTGCATCCC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGCAATAGGCTTTGGCTGC'}, {'score': 0, 'version': 2L, 'sequence': 'GAGCAATAGGCTTTGGCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTAGGTTTTCTCTGACCC'}, {'score': 66, 'version': 2L, 'sequence': 'TTCTAGGTTTTCTCTGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTAGGTTTTCTCTGACCC'}, {'score': 66, 'version': 2L, 'sequence': 'TTCTAGGTTTTCTCTGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCATAGATGTCTCCCCC'}, {'score': 28, 'version': 2L, 'sequence': 'AGTGCATAGATGTCTCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCATAGATGTCTCCCCC'}, {'score': 28, 'version': 2L, 'sequence': 'AGTGCATAGATGTCTCCCCC'})
({'score': 39, 'version': 0L, 'sequence': 'CTGTAGCTGTTCAATATGGC'}, {'score': 89, 'version': 2L, 'sequence': 'CTGTAGCTGTTCAATATGGC'})
({'score': 99, 'version': 0L, 'sequence': 'CCCACCCTACCTTGCCCCGC'}, {'score': 33, 'version': 2L, 'sequence': 'CCCACCCTACCTTGCCCCGC'})
({'score': 100, 'version': 0L, 'sequence': 'CACCTACATGATGGAGTCCC'}, {'score': 97, 'version': 2L, 'sequence': 'CACCTACATGATGGAGTCCC'})
({'score': 98, 'version': 0L, 'sequence': 'TTCCAGTTTAAATTCCAGAC'}, {'score': 92, 'version': 2L, 'sequence': 'TTCCAGTTTAAATTCCAGAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACTCAAATGAACAATGCC'}, {'score': 66, 'version': 2L, 'sequence': 'TCACTCAAATGAACAATGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GACTAAGGCTTTAGATACAC'}, {'score': 95, 'version': 2L, 'sequence': 'GACTAAGGCTTTAGATACAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGAAGCCATTGTTTAACC'}, {'score': 72, 'version': 2L, 'sequence': 'AAAGAAGCCATTGTTTAACC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACTCAAATGAACAATGCC'}, {'score': 66, 'version': 2L, 'sequence': 'TCACTCAAATGAACAATGCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATCTAAGGCTTTAGATACAC'}, {'score': 95, 'version': 2L, 'sequence': 'ATCTAAGGCTTTAGATACAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGAAGCCATTTTTTAACC'}, {'score': 72, 'version': 2L, 'sequence': 'AAAGAAGCCATTTTTTAACC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAAACCATGAGATCAATAC'}, {'score': 79, 'version': 2L, 'sequence': 'TAAAACCATGAGATCAATAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAGGGGAGTTACTGGATTC'}, {'score': 63, 'version': 2L, 'sequence': 'CCAGGGGAGTTACTGGATTC'})
({'score': 100, 'version': 0L, 'sequence': 'AATCTACATGGCTGCTGCTC'}, {'score': 99, 'version': 2L, 'sequence': 'AATCTACATGGCTGCTGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAAAAACTGGGACAAAAAC'}, {'score': 83, 'version': 2L, 'sequence': 'AAAAAAACTGGGACAAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GATAAATTAGGTCACAAAAC'}, {'score': 98, 'version': 2L, 'sequence': 'GATAAATTAGGTCACAAAAC'})
({'score': 99, 'version': 0L, 'sequence': 'CAATTAAAATGGGACAAGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CAATTAAAATGGGACAAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTGCTTAGTAGTGCCACC'}, {'score': 95, 'version': 2L, 'sequence': 'TCCTGCTTAGTAGTGCCACC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGAGGAAACACAGAGCTAC'}, {'score': 3, 'version': 2L, 'sequence': 'GTGAGGAAACACAGAGCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATATATTAGCTGCTATGC'}, {'score': 93, 'version': 2L, 'sequence': 'ATATATATTAGCTGCTATGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTGGTAAATGCATAAGAAC'}, {'score': 95, 'version': 2L, 'sequence': 'GCTGGTAAATGCATAAGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCAGGCTGGGGCGGATTAAC'}, {'score': 97, 'version': 2L, 'sequence': 'GCAGGCTGGGGCGGATTAAC'})
({'score': 98, 'version': 0L, 'sequence': 'ATCTATAATGCAGGTATGAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCTATAATGCAGGTATGAC'})
({'score': 100, 'version': 0L, 'sequence': 'AATGGGCCCCTTATATGTCC'}, {'score': 98, 'version': 2L, 'sequence': 'AATGGGCCCCTTATATGTCC'})
({'score': 99, 'version': 0L, 'sequence': 'GAAGAAGCCAACCTTGCAGC'}, {'score': 100, 'version': 2L, 'sequence': 'GAAGAAGCCAACCTTGCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CATACTGTTACTTCATACAC'}, {'score': 94, 'version': 2L, 'sequence': 'CATACTGTTACTTCATACAC'})
({'score': 97, 'version': 0L, 'sequence': 'CTCATTAGTACAAAGTTAGC'}, {'score': 100, 'version': 2L, 'sequence': 'CTCATTAGTACAAAGTTAGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGGACAATGGGTTTTAAC'}, {'score': 96, 'version': 2L, 'sequence': 'AAAGGACAATGGGTTTTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTACCTGTACTCTGGGTCTC'}, {'score': 99, 'version': 2L, 'sequence': 'TTACCTGTACTCTGGGTCTC'})
({'score': 100, 'version': 0L, 'sequence': 'TAACTCAATGAACAGGCTGC'}, {'score': 97, 'version': 2L, 'sequence': 'TAACTCAATGAACAGGCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAACCCAACTCTAAAAATTC'}, {'score': 99, 'version': 2L, 'sequence': 'AAACCCAACTCTAAAAATTC'})
({'score': 62, 'version': 0L, 'sequence': 'ACTTGCAAATTGGTTGCAGC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTTGCAAATTGGTTGCAGC'})
({'score': 99, 'version': 0L, 'sequence': 'ACTGCTAATATCTTAAAAAC'}, {'score': 3, 'version': 2L, 'sequence': 'ACTGCTAATATCTTAAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATACATTGCTTTTATTTC'}, {'score': 88, 'version': 2L, 'sequence': 'ATATACATTGCTTTTATTTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATGGGGAGGGGGCGGGGCCC'}, {'score': 89, 'version': 2L, 'sequence': 'ATGGGGAGGGGGCGGGGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCCTTGGCTGCTATTTTAC'}, {'score': 2, 'version': 2L, 'sequence': 'GGCCTTGGCTGCTATTTTAC'})
({'score': 73, 'version': 0L, 'sequence': 'GCCTGGGATTGCCACCTGGC'}, {'score': 74, 'version': 2L, 'sequence': 'GCCTGGGATTGCCACCTGGC'})
({'score': 83, 'version': 0L, 'sequence': 'AAATACCAGTGACAGACAGC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATACCAGTGACAGACAGC'})
({'score': 99, 'version': 0L, 'sequence': 'CAGTTATTTTTTTATTCTAC'}, {'score': 97, 'version': 2L, 'sequence': 'CAGTTATTTTTTTATTCTAC'})
({'score': 99, 'version': 0L, 'sequence': 'ATATAATCTGGCCCTCAGAC'}, {'score': 98, 'version': 2L, 'sequence': 'ATATAATCTGGCCCTCAGAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATACCCATATTAACCAATCC'}, {'score': 99, 'version': 2L, 'sequence': 'ATACCCATATTAACCAATCC'})
({'score': 99, 'version': 0L, 'sequence': 'CAAAACATAAGCCAGAAATC'}, {'score': 100, 'version': 2L, 'sequence': 'CAAAACATAAGCCAGAAATC'})
({'score': 99, 'version': 0L, 'sequence': 'AAGGTGACATTTTGGAGGTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGGTGACATTTTGGAGGTC'})
({'score': 99, 'version': 0L, 'sequence': 'TGATGGAACATATCCTGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TGATGGAACATATCCTGTTC'})
({'score': 95, 'version': 0L, 'sequence': 'CTTGATTTGTAACCCAAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'CTTGATTTGTAACCCAAAGC'})
({'score': 64, 'version': 0L, 'sequence': 'ATATATATATATATATCCAC'}, {'score': 4, 'version': 2L, 'sequence': 'ATATATATATATATATCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCAGCTCTATGGGACAGATC'}, {'score': 99, 'version': 2L, 'sequence': 'GCAGCTCTATGGGACAGATC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCTCTGATGGTACCAAGTC'}, {'score': 72, 'version': 2L, 'sequence': 'AGCTCTGATGGTACCAAGTC'})
({'score': 97, 'version': 0L, 'sequence': 'TGGTACAGTCCCTATGTGAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGTACAGTCCCTATGTGAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCCATTCTGCCTTTTTTTC'}, {'score': 97, 'version': 2L, 'sequence': 'TTCCATTCTGCCTTTTTTTC'})
({'score': 92, 'version': 0L, 'sequence': 'TATAGAGAAGGGGGGGGGAC'}, {'score': 93, 'version': 2L, 'sequence': 'TATAGAGAAGGGGGGGGGAC'})
({'score': 99, 'version': 0L, 'sequence': 'CAGGTTGAGAAGGGGTGAAC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGGTTGAGAAGGGGTGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGCTATTGCCCCTGCCAC'}, {'score': 89, 'version': 2L, 'sequence': 'TTTGCTATTGCCCCTGCCAC'})
({'score': 43, 'version': 0L, 'sequence': 'ACAGCAGGAGGGCTGCGGGC'}, {'score': 62, 'version': 2L, 'sequence': 'ACAGCAGGAGGGCTGCGGGC'})
({'score': 97, 'version': 0L, 'sequence': 'GATACCAATGTAAGCCCAGC'}, {'score': 100, 'version': 2L, 'sequence': 'GATACCAATGTAAGCCCAGC'})
({'score': 13, 'version': 0L, 'sequence': 'GATACCCCAGAAAGTCCAGC'}, {'score': 33, 'version': 2L, 'sequence': 'GATACCCCAGAAAGTCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTCAACCTAACTAACCTTC'}, {'score': 12, 'version': 2L, 'sequence': 'TCTCAACCTAACTAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTCAACCTAACTAACCTTC'}, {'score': 12, 'version': 2L, 'sequence': 'TCTCAACCTAACTAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCAACCTAACTAACCTTC'}, {'score': 24, 'version': 2L, 'sequence': 'GCTCAACCTAACTAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCAACCTAACTAACCTTC'}, {'score': 24, 'version': 2L, 'sequence': 'GCTCAACCTAACTAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTCACGCTAACTGACCTTC'}, {'score': 1, 'version': 2L, 'sequence': 'TCTCACGCTAACTGACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGCTGGTATTTAACTGGCC'}, {'score': 2, 'version': 2L, 'sequence': 'TGGCTGGTATTTAACTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGTCAGTAAATCCCCAAC'}, {'score': 99, 'version': 2L, 'sequence': 'TAGTCAGTAAATCCCCAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTAATACCAGTACCGGCTC'}, {'score': 65, 'version': 2L, 'sequence': 'TGTAATACCAGTACCGGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGAGGGCAGTCCTGCCTAC'}, {'score': 96, 'version': 2L, 'sequence': 'AAGAGGGCAGTCCTGCCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTTGTGTGAATATCACATC'}, {'score': 95, 'version': 2L, 'sequence': 'CCTTGTGTGAATATCACATC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAATTTGATCATTTGGCCC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATTTGATCATTTGGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAATTTGATCATTTGGCCC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATTTGATCATTTGGCCC'})
({'score': 5, 'version': 0L, 'sequence': 'CTTTTATTACATAACCCCAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTTTTATTACATAACCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTCTGTTATAAAGATAGC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCTCTGTTATAAAGATAGC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAATTCCACCAAAACCCAC'}, {'score': 6, 'version': 2L, 'sequence': 'GAAATTCCACCAAAACCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTCATTCACACCACTGCC'}, {'score': 0, 'version': 2L, 'sequence': 'CTCTCATTCACACCACTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGCACACAATCGAATATTC'}, {'score': 19, 'version': 2L, 'sequence': 'GAGCACACAATCGAATATTC'})
({'score': 95, 'version': 0L, 'sequence': 'GCACTCTGCAGCTCCAACCC'}, {'score': 13, 'version': 2L, 'sequence': 'GCACTCTGCAGCTCCAACCC'})
({'score': 89, 'version': 0L, 'sequence': 'TTACTGCTCCTGGGCAAATC'}, {'score': 0, 'version': 2L, 'sequence': 'TTACTGCTCCTGGGCAAATC'})
({'score': 99, 'version': 0L, 'sequence': 'GATTAGTTGCTATGGGTTAC'}, {'score': 0, 'version': 2L, 'sequence': 'GATTAGTTGCTATGGGTTAC'})
({'score': 99, 'version': 0L, 'sequence': 'GATTAGTTGCTATGGGTTAC'}, {'score': 0, 'version': 2L, 'sequence': 'GATTAGTTGCTATGGGTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'}, {'score': 16, 'version': 2L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCAATAGTTATTTGGCTGC'}, {'score': 0, 'version': 2L, 'sequence': 'AGCAATAGTTATTTGGCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCAATAGTTATTTGGCTGC'}, {'score': 0, 'version': 2L, 'sequence': 'AGCAATAGTTATTTGGCTGC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTCTGTTATAAAGATAGC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCTCTGTTATAAAGATAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAATTTGATCATTTGGCCC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATTTGATCATTTGGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CGTTGATGCGGTCCCCGAAC'}, {'score': 47, 'version': 2L, 'sequence': 'CGTTGATGCGGTCCCCGAAC'})
({'score': 33, 'version': 0L, 'sequence': 'TAACTGCTGAAATTCCAAAC'}, {'score': 0, 'version': 2L, 'sequence': 'TAACTGCTGAAATTCCAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'}, {'score': 16, 'version': 2L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGAACATGTAAGTCGC'}, {'score': 93, 'version': 2L, 'sequence': 'GCGAACATGTAAGTCGC'})
({'score': 33, 'version': 0L, 'sequence': 'TAACTGCTGAAATTCCAAAC'}, {'score': 0, 'version': 2L, 'sequence': 'TAACTGCTGAAATTCCAAAC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 99, 'version': 0L, 'sequence': 'TATCCAGAATGCTTGTGACC'}, {'score': 0, 'version': 2L, 'sequence': 'TATCCAGAATGCTTGTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'CCACGATAAGTAGCTGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CCACGATAAGTAGCTGCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGGCACCCTCGGCTTATAC'}, {'score': 6, 'version': 2L, 'sequence': 'AAGGCACCCTCGGCTTATAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCATGAAAAGTAGCTGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CCATGAAAAGTAGCTGCTAC'})
({'score': 0, 'version': 0L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'})
({'score': 1, 'version': 0L, 'sequence': 'CTAATATCTACAGTTAGTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATATCTACAGTTAGTAC'})
({'score': 0, 'version': 0L, 'sequence': 'CTGGGATTTAAAATAGGCCC'}, {'score': 3, 'version': 2L, 'sequence': 'CTGGGATTTAAAATAGGCCC'})
({'score': 0, 'version': 0L, 'sequence': 'CTGGGATTTAAAATAGGCCC'}, {'score': 3, 'version': 2L, 'sequence': 'CTGGGATTTAAAATAGGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCAATGCCATCCCACTGGC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCAATGCCATCCCACTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCACCGGCAATTTACATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CCCACCGGCAATTTACATTC'})
({'score': 0, 'version': 0L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'}, {'score': 16, 'version': 2L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'})
({'score': 0, 'version': 0L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCATGATAAGTAGCTGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CCATGATAAGTAGCTGCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCCAGGAAGTTTCAGCGGC'}, {'score': 6, 'version': 2L, 'sequence': 'TTCCAGGAAGTTTCAGCGGC'})
({'score': 92, 'version': 0L, 'sequence': 'CCACGACAAGTAGCCGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CCACGACAAGTAGCCGCTAC'})
({'score': 0, 'version': 0L, 'sequence': 'CAAATTGAGGAATATTGGCC'}, {'score': 87, 'version': 2L, 'sequence': 'CAAATTGAGGAATATTGGCC'})
({'score': 0, 'version': 0L, 'sequence': 'CAAATTGAGGAATATTGGCC'}, {'score': 87, 'version': 2L, 'sequence': 'CAAATTGAGGAATATTGGCC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATCCGCGGTAAGGCGTTTTC'}, {'score': 0, 'version': 2L, 'sequence': 'ATCCGCGGTAAGGCGTTTTC'})
({'score': 0, 'version': 0L, 'sequence': 'CTGGGATTTAAAATAGGCCC'}, {'score': 3, 'version': 2L, 'sequence': 'CTGGGATTTAAAATAGGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CGAAATGCCATCCCACCGAC'}, {'score': 2, 'version': 2L, 'sequence': 'CGAAATGCCATCCCACCGAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCACCGGCAATTTACATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CCCACCGGCAATTTACATTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACACCACTGCCTGGTTGC'}, {'score': 0, 'version': 2L, 'sequence': 'TCACACCACTGCCTGGTTGC'})
({'score': 20, 'version': 0L, 'sequence': 'AGTAAATCACCAGCTAGGGC'}, {'score': 0, 'version': 2L, 'sequence': 'AGTAAATCACCAGCTAGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'GACTACCAAATGGTTTCATC'}, {'score': 98, 'version': 2L, 'sequence': 'GACTACCAAATGGTTTCATC'})
({'score': 100, 'version': 0L, 'sequence': 'ACAGCTGTGTAATAGGTTTC'}, {'score': 95, 'version': 2L, 'sequence': 'ACAGCTGTGTAATAGGTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAGAGGCAGGGGTGCTTAC'}, {'score': 99, 'version': 2L, 'sequence': 'TCAGAGGCAGGGGTGCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACAGTGTCAGACTGGGCGTC'}, {'score': 99, 'version': 2L, 'sequence': 'ACAGTGTCAGACTGGGCGTC'})
({'score': 99, 'version': 0L, 'sequence': 'TTTTCAGCATAAAATTACAC'}, {'score': 96, 'version': 2L, 'sequence': 'TTTTCAGCATAAAATTACAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTGTGCAGGTACCCTGAGC'}, {'score': 95, 'version': 2L, 'sequence': 'TCTGTGCAGGTACCCTGAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGGGATTCGGCCGAATCC'}, {'score': 3, 'version': 2L, 'sequence': 'TTTGGGATTCGGCCGAATCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGGGATTCGGCCGAATCC'}, {'score': 3, 'version': 2L, 'sequence': 'TTTGGGATTCGGCCGAATCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTAAGCATTCGGTTTCGGC'}, {'score': 3, 'version': 2L, 'sequence': 'TTTAAGCATTCGGTTTCGGC'})
({'score': 100, 'version': 0L, 'sequence': 'GAATTGGGATTTGGTTCATC'}, {'score': 91, 'version': 2L, 'sequence': 'GAATTGGGATTTGGTTCATC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTAATTCCCCCCCCCCCCC'}, {'score': 50, 'version': 2L, 'sequence': 'AGTAATTCCCCCCCCCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTAATTCCCCCCCCCCCCC'}, {'score': 50, 'version': 2L, 'sequence': 'AGTAATTCCCCCCCCCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGTGCAAACAGCACCTACC'}, {'score': 99, 'version': 2L, 'sequence': 'CTGTGCAAACAGCACCTACC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGTGCAAACAGCACCTACC'}, {'score': 99, 'version': 2L, 'sequence': 'CTGTGCAAACAGCACCTACC'})
({'score': 79, 'version': 0L, 'sequence': 'GGCGCTACAGATTGCCAGTC'}, {'score': 82, 'version': 2L, 'sequence': 'GGCGCTACAGATTGCCAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTATAATATCAACAAACAGC'}, {'score': 93, 'version': 2L, 'sequence': 'TTATAATATCAACAAACAGC'})
({'score': 98, 'version': 0L, 'sequence': 'TGTCAGAGAGGAGTTGGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTCAGAGAGGAGTTGGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCCTTTGAATGGCTGCCTC'}, {'score': 98, 'version': 2L, 'sequence': 'CTCCTTTGAATGGCTGCCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCACTGATCCCTGGGCCCAC'}, {'score': 0, 'version': 2L, 'sequence': 'CCACTGATCCCTGGGCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAGGGGGGGTCACTGACCC'}, {'score': 11, 'version': 2L, 'sequence': 'ATAGGGGGGGTCACTGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAGGGGGGGTCACTGACCC'}, {'score': 11, 'version': 2L, 'sequence': 'ATAGGGGGGGTCACTGACCC'})
({'score': 10, 'version': 0L, 'sequence': 'ATTATACTGACCTTTGGGTC'}, {'score': 12, 'version': 2L, 'sequence': 'ATTATACTGACCTTTGGGTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTACTGGCAGTTATAATGC'}, {'score': 99, 'version': 2L, 'sequence': 'TCTACTGGCAGTTATAATGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCGCCGTGTGCCATCCCAC'}, {'score': 27, 'version': 2L, 'sequence': 'GCCGCCGTGTGCCATCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGAACATGTAAGTCGC'}, {'score': 93, 'version': 2L, 'sequence': 'GCGAACATGTAAGTCGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCCCGAACTACCATCCCTC'}, {'score': 12, 'version': 2L, 'sequence': 'TCCCCGAACTACCATCCCTC'})
({'score': 93, 'version': 0L, 'sequence': 'TAATTCTGTTTGATGCGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TAATTCTGTTTGATGCGGCC'})
({'score': 93, 'version': 0L, 'sequence': 'TAATTCTGTTTGATGCGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TAATTCTGTTTGATGCGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'AATCTCTGCTCCTTACATGC'}, {'score': 64, 'version': 2L, 'sequence': 'AATCTCTGCTCCTTACATGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGCACCTGACTTATAAATC'}, {'score': 33, 'version': 2L, 'sequence': 'GGGCACCTGACTTATAAATC'})
({'score': 99, 'version': 0L, 'sequence': 'CAGCTCCTATAATGGGAGGC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGCTCCTATAATGGGAGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTATATCAAACACAAGCAC'}, {'score': 96, 'version': 2L, 'sequence': 'TCTATATCAAACACAAGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTACGGAAGGACCCCTTAAC'}, {'score': 3, 'version': 2L, 'sequence': 'TTACGGAAGGACCCCTTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGCTTTGTATGTTTGCAC'}, {'score': 94, 'version': 2L, 'sequence': 'TTTGCTTTGTATGTTTGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGATTGAGGCCATTTTGGC'}, {'score': 99, 'version': 2L, 'sequence': 'CTGATTGAGGCCATTTTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGTTTGTTTAAGAAGCAC'}, {'score': 98, 'version': 2L, 'sequence': 'TTTGTTTGTTTAAGAAGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCGATGCAGCCCCCCCCTC'}, {'score': 99, 'version': 2L, 'sequence': 'AGCGATGCAGCCCCCCCCTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAATCTCATTATCTGTTGGC'}, {'score': 50, 'version': 2L, 'sequence': 'GAATCTCATTATCTGTTGGC'})
({'score': 98, 'version': 0L, 'sequence': 'GAATATGAATAGGATAGCAC'}, {'score': 45, 'version': 2L, 'sequence': 'GAATATGAATAGGATAGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACATTTCATTGGTTTCCC'}, {'score': 95, 'version': 2L, 'sequence': 'TCACATTTCATTGGTTTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACATTTCATTGGTTTCCC'}, {'score': 95, 'version': 2L, 'sequence': 'TCACATTTCATTGGTTTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGGGTATTCCAAGGGAATC'}, {'score': 94, 'version': 2L, 'sequence': 'GTGGGTATTCCAAGGGAATC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGAATGAAAAGTAATAAC'}, {'score': 98, 'version': 2L, 'sequence': 'TTTGAATGAAAAGTAATAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGATTTTTATTTTATTCTGC'}, {'score': 86, 'version': 2L, 'sequence': 'TGATTTTTATTTTATTCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCTGTGTATGGGCCCCCAC'}, {'score': 31, 'version': 2L, 'sequence': 'GCCTGTGTATGGGCCCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TATATATGTATAAAATTGGC'}, {'score': 49, 'version': 2L, 'sequence': 'TATATATGTATAAAATTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCAATAGTTTATGTGCTGC'}, {'score': 9, 'version': 2L, 'sequence': 'AGCAATAGTTTATGTGCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATATACATAAACCATCCC'}, {'score': 99, 'version': 2L, 'sequence': 'ATATATACATAAACCATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCAGGCAGCCATCTGGAAC'}, {'score': 96, 'version': 2L, 'sequence': 'TGCAGGCAGCCATCTGGAAC'})
({'score': 99, 'version': 0L, 'sequence': 'CAATGGGTGGTGGAACACTC'}, {'score': 100, 'version': 2L, 'sequence': 'CAATGGGTGGTGGAACACTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTATCCTGTTTCGCTTTAC'}, {'score': 99, 'version': 2L, 'sequence': 'TCTATCCTGTTTCGCTTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGAACTTTTGTGAATTTTC'}, {'score': 85, 'version': 2L, 'sequence': 'GTGAACTTTTGTGAATTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTAATCTATTTTTTTATC'}, {'score': 91, 'version': 2L, 'sequence': 'TTTTAATCTATTTTTTTATC'})
({'score': 59, 'version': 0L, 'sequence': 'AAAAATGACACATTTTGGTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAAATGACACATTTTGGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GGAGTGCGTTGAGTGCATGC'}, {'score': 1, 'version': 2L, 'sequence': 'GGAGTGCGTTGAGTGCATGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATCAGTTGTAGAAACATCAC'}, {'score': 2, 'version': 2L, 'sequence': 'ATCAGTTGTAGAAACATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CGGGTCTGGGGCATCGCACC'}, {'score': 3, 'version': 2L, 'sequence': 'CGGGTCTGGGGCATCGCACC'})
({'score': 100, 'version': 0L, 'sequence': 'CGGGTCTGGGGCATCGCACC'}, {'score': 3, 'version': 2L, 'sequence': 'CGGGTCTGGGGCATCGCACC'})
({'score': 3, 'version': 0L, 'sequence': 'GGTACTCTGGTAGGCCAGTC'}, {'score': 36, 'version': 2L, 'sequence': 'GGTACTCTGGTAGGCCAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCGATCCCTGCTGGGAACTC'}, {'score': 35, 'version': 2L, 'sequence': 'CCGATCCCTGCTGGGAACTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTGTGTTTGTATTGGTAC'}, {'score': 98, 'version': 2L, 'sequence': 'TCCTGTGTTTGTATTGGTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAGCGTTGCTTCTCAACAC'}, {'score': 75, 'version': 2L, 'sequence': 'TAAGCGTTGCTTCTCAACAC'})
({'score': 3, 'version': 0L, 'sequence': 'GTGCTCAGTGGGCTCTGGCC'}, {'score': 56, 'version': 2L, 'sequence': 'GTGCTCAGTGGGCTCTGGCC'})
({'score': 3, 'version': 0L, 'sequence': 'GTGCTCAGTGGGCTCTGGCC'}, {'score': 56, 'version': 2L, 'sequence': 'GTGCTCAGTGGGCTCTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCCCCAAGAACATGACATC'}, {'score': 95, 'version': 2L, 'sequence': 'CCCCCCAAGAACATGACATC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGCCCACCAGGATTTTCCC'}, {'score': 25, 'version': 2L, 'sequence': 'GAGCCCACCAGGATTTTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GATAACTCAACTACAGGCAC'}, {'score': 99, 'version': 2L, 'sequence': 'GATAACTCAACTACAGGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTCATGCCCCTCCAGCCGC'}, {'score': 97, 'version': 2L, 'sequence': 'TTTCATGCCCCTCCAGCCGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGTGTCTGTTACACAGAC'}, {'score': 97, 'version': 2L, 'sequence': 'ATTGTGTCTGTTACACAGAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAAATAGAAATCTTTGCGC'}, {'score': 99, 'version': 2L, 'sequence': 'AAAAATAGAAATCTTTGCGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGGCAATACTACTTGGCC'}, {'score': 4, 'version': 2L, 'sequence': 'AAAGGCAATACTACTTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGGCAATACTACTTGGCC'}, {'score': 4, 'version': 2L, 'sequence': 'AAAGGCAATACTACTTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCAGCTTAAAAAGTTGTGC'}, {'score': 97, 'version': 2L, 'sequence': 'TGCAGCTTAAAAAGTTGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AATTTATGCCTCCCTAGGCC'}, {'score': 1, 'version': 2L, 'sequence': 'AATTTATGCCTCCCTAGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'AATTTATGCCTCCCTAGGCC'}, {'score': 1, 'version': 2L, 'sequence': 'AATTTATGCCTCCCTAGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CGGGCCTTGCCACATATC'}, {'score': 99, 'version': 2L, 'sequence': 'CGGGCCTTGCCACATATC'})
({'score': 46, 'version': 0L, 'sequence': 'TAGGGGGGTGCAGGGCAAAC'}, {'score': 47, 'version': 2L, 'sequence': 'TAGGGGGGTGCAGGGCAAAC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGTAAATGCCAAACAAGTC'}, {'score': 98, 'version': 2L, 'sequence': 'TGGTAAATGCCAAACAAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGCCATACTGTCTTCTTGC'}, {'score': 95, 'version': 2L, 'sequence': 'CTGCCATACTGTCTTCTTGC'})
({'score': 99, 'version': 0L, 'sequence': 'TACCCGCACCCAGCGCTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TACCCGCACCCAGCGCTCCC'})
({'score': 99, 'version': 0L, 'sequence': 'TACCCGCACCCAGCGCTCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TACCCGCACCCAGCGCTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCCTCCCTCTATCCCTGTC'}, {'score': 98, 'version': 2L, 'sequence': 'CCCCTCCCTCTATCCCTGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGGAGTGAGAGCGCCTCCC'}, {'score': 97, 'version': 2L, 'sequence': 'GGGGAGTGAGAGCGCCTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGGAGTGAGAGCGCCTCCC'}, {'score': 97, 'version': 2L, 'sequence': 'GGGGAGTGAGAGCGCCTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCACATGTGCAGCCCCAGCC'}, {'score': 98, 'version': 2L, 'sequence': 'CCACATGTGCAGCCCCAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACTGCCCTTTCCTTCCCC'}, {'score': 97, 'version': 2L, 'sequence': 'TCACTGCCCTTTCCTTCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACTGCCCTTTCCTTCCCC'}, {'score': 97, 'version': 2L, 'sequence': 'TCACTGCCCTTTCCTTCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTGCTAGGGTGGAAGATGGC'}, {'score': 97, 'version': 2L, 'sequence': 'TTGCTAGGGTGGAAGATGGC'})
({'score': 1, 'version': 0L, 'sequence': 'CTAATATCTACAGTTAGTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATATCTACAGTTAGTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATCAATTTATGGTTAGCACC'}, {'score': 11, 'version': 2L, 'sequence': 'ATCAATTTATGGTTAGCACC'})
({'score': 100, 'version': 0L, 'sequence': 'ATCAATTTATGGTTAGCACC'}, {'score': 11, 'version': 2L, 'sequence': 'ATCAATTTATGGTTAGCACC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAAAATGACGGATTAGAAC'}, {'score': 50, 'version': 2L, 'sequence': 'CCAAAATGACGGATTAGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTTTAATATTTTGGTGCGC'}, {'score': 1, 'version': 2L, 'sequence': 'ACTTTAATATTTTGGTGCGC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGGGGCGGCATGCCGCCC'}, {'score': 33, 'version': 2L, 'sequence': 'TAGGGGGCGGCATGCCGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGGGGCGGCATGCCGCCC'}, {'score': 33, 'version': 2L, 'sequence': 'TAGGGGGCGGCATGCCGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCCTGCCCGCCCTGGC'}, {'score': 96, 'version': 2L, 'sequence': 'GCCCTGCCCGCCCTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGAACTGCATTTCTTTTACC'}, {'score': 94, 'version': 2L, 'sequence': 'TGAACTGCATTTCTTTTACC'})
({'score': 100, 'version': 0L, 'sequence': 'TGAACTGCATTTCTTTTACC'}, {'score': 94, 'version': 2L, 'sequence': 'TGAACTGCATTTCTTTTACC'})
({'score': 100, 'version': 0L, 'sequence': 'CATTAACACAACATCCACAC'}, {'score': 97, 'version': 2L, 'sequence': 'CATTAACACAACATCCACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTGTTGTGTAATGAACGC'}, {'score': 99, 'version': 2L, 'sequence': 'GTGTGTTGTGTAATGAACGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATAACTTTATATACAAGC'}, {'score': 97, 'version': 2L, 'sequence': 'ATATAACTTTATATACAAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TATCGGTTGCCTCGTTGACC'}, {'score': 94, 'version': 2L, 'sequence': 'TATCGGTTGCCTCGTTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'TATCGGTTGCCTCGTTGACC'}, {'score': 94, 'version': 2L, 'sequence': 'TATCGGTTGCCTCGTTGACC'})
({'score': 62, 'version': 0L, 'sequence': 'CTGCTTTATGGTTGAGATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CTGCTTTATGGTTGAGATTC'})
({'score': 62, 'version': 0L, 'sequence': 'CTGCTTTATGGTTGAGATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CTGCTTTATGGTTGAGATTC'})
({'score': 62, 'version': 0L, 'sequence': 'CTGCTTTATGGTTGAGATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CTGCTTTATGGTTGAGATTC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGGATTGGATCTGGGCCGC'}, {'score': 2, 'version': 2L, 'sequence': 'GGGGATTGGATCTGGGCCGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCGAAACCCAAACCGAATC'}, {'score': 2, 'version': 2L, 'sequence': 'CCCGAAACCCAAACCGAATC'})
({'score': 100, 'version': 0L, 'sequence': 'GCAGTCTCGGACTGGCCCAC'}, {'score': 0, 'version': 2L, 'sequence': 'GCAGTCTCGGACTGGCCCAC'})
({'score': 43, 'version': 0L, 'sequence': 'GGAGTTTTCCTGGTATCC'}, {'score': 97, 'version': 2L, 'sequence': 'GGAGTTTTCCTGGTATCC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGAGGTCCCCCTGTTGTGC'}, {'score': 97, 'version': 2L, 'sequence': 'TAGAGGTCCCCCTGTTGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGCAGATTCTCACAGGGCC'}, {'score': 34, 'version': 2L, 'sequence': 'AAGCAGATTCTCACAGGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGCAGATTCTCACAGGGCC'}, {'score': 34, 'version': 2L, 'sequence': 'AAGCAGATTCTCACAGGGCC'})
({'score': 97, 'version': 0L, 'sequence': 'CAGAGTACCAGAGAATAGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGAGTACCAGAGAATAGCC'})
({'score': 97, 'version': 0L, 'sequence': 'CAGAGTACCAGAGAATAGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGAGTACCAGAGAATAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTATCGGACTCTATCGGACC'}, {'score': 99, 'version': 2L, 'sequence': 'GTATCGGACTCTATCGGACC'})
({'score': 100, 'version': 0L, 'sequence': 'GTATCGGACTCTATCGGACC'}, {'score': 99, 'version': 2L, 'sequence': 'GTATCGGACTCTATCGGACC'})
({'score': 45, 'version': 0L, 'sequence': 'GCTTTCTGGATAACGGTTTC'}, {'score': 1, 'version': 2L, 'sequence': 'GCTTTCTGGATAACGGTTTC'})
({'score': 13, 'version': 0L, 'sequence': 'ACAATCAGGGTAGGGTAGGC'}, {'score': 79, 'version': 2L, 'sequence': 'ACAATCAGGGTAGGGTAGGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGATAAGGGGTCCGATACC'}, {'score': 17, 'version': 2L, 'sequence': 'AGGATAAGGGGTCCGATACC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGATAAGGGGTCCGATACC'}, {'score': 17, 'version': 2L, 'sequence': 'AGGATAAGGGGTCCGATACC'})
({'score': 100, 'version': 0L, 'sequence': 'CATGACTGGGGCAGGGGCCC'}, {'score': 99, 'version': 2L, 'sequence': 'CATGACTGGGGCAGGGGCCC'})
({'score': 92, 'version': 0L, 'sequence': 'TGGGACATCAGGAAAAAACC'}, {'score': 1, 'version': 2L, 'sequence': 'TGGGACATCAGGAAAAAACC'})
({'score': 92, 'version': 0L, 'sequence': 'TGGGACATCAGGAAAAAACC'}, {'score': 1, 'version': 2L, 'sequence': 'TGGGACATCAGGAAAAAACC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCATCTGTTGGTGCAACAC'}, {'score': 96, 'version': 2L, 'sequence': 'TGCATCTGTTGGTGCAACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCACTATCAGCCCAGCCCCC'}, {'score': 98, 'version': 2L, 'sequence': 'GCACTATCAGCCCAGCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCACTATCAGCCCAGCCCCC'}, {'score': 98, 'version': 2L, 'sequence': 'GCACTATCAGCCCAGCCCCC'})
({'score': 44, 'version': 0L, 'sequence': 'GGGACAGAGCTGGGCTGTGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGACAGAGCTGGGCTGTGC'})
({'score': 6, 'version': 0L, 'sequence': 'CGGCTCGTTAAAGCGGTCCC'}, {'score': 9, 'version': 2L, 'sequence': 'CGGCTCGTTAAAGCGGTCCC'})
({'score': 6, 'version': 0L, 'sequence': 'CGGCTCGTTAAAGCGGTCCC'}, {'score': 9, 'version': 2L, 'sequence': 'CGGCTCGTTAAAGCGGTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTAAATGGGGTCACTGACTC'}, {'score': 40, 'version': 2L, 'sequence': 'TTAAATGGGGTCACTGACTC'})
({'score': 100, 'version': 0L, 'sequence': 'ACCAAAAGTTTTTTGGCTGC'}, {'score': 6, 'version': 2L, 'sequence': 'ACCAAAAGTTTTTTGGCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCCCTGAAGTTGCCCCCAC'}, {'score': 14, 'version': 2L, 'sequence': 'GGCCCTGAAGTTGCCCCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGTGCTTGCACGGAGTAAC'}, {'score': 96, 'version': 2L, 'sequence': 'CTGTGCTTGCACGGAGTAAC'})
({'score': 0, 'version': 0L, 'sequence': 'AAGGTTGGGGACCCCTGCTC'}, {'score': 8, 'version': 2L, 'sequence': 'AAGGTTGGGGACCCCTGCTC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 1, 'version': 0L, 'sequence': 'TGTTAGGTTAAATTGGACCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTTAGGTTAAATTGGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAATTTGATCATTTGGCCC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATTTGATCATTTGGCCC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAATTTCAACCCTTTCCAC'}, {'score': 3, 'version': 2L, 'sequence': 'TAAATTTCAACCCTTTCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCGCATCTGTTCTGGTGAAC'}, {'score': 3, 'version': 2L, 'sequence': 'CCGCATCTGTTCTGGTGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCACCGCTCTAGGACCTCC'}, {'score': 3, 'version': 2L, 'sequence': 'TGCACCGCTCTAGGACCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCACCGCTCTAGGACCTCC'}, {'score': 3, 'version': 2L, 'sequence': 'TGCACCGCTCTAGGACCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTGGGAAGACTGCGGGGCC'}, {'score': 1, 'version': 2L, 'sequence': 'CCTGGGAAGACTGCGGGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTAGTCTGCTGCTTTTATCC'}, {'score': 3, 'version': 2L, 'sequence': 'TTAGTCTGCTGCTTTTATCC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAAGCAGCAGACTAAATCC'}, {'score': 3, 'version': 2L, 'sequence': 'AAAAGCAGCAGACTAAATCC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAAGCAGCAGACTAAATCC'}, {'score': 3, 'version': 2L, 'sequence': 'AAAAGCAGCAGACTAAATCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTGATAATGCCGAACGGTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TTGATAATGCCGAACGGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTCTACCTTCTTGCGGGGC'}, {'score': 3, 'version': 2L, 'sequence': 'TCTCTACCTTCTTGCGGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAAGGCCTACAGCCTGGCC'}, {'score': 3, 'version': 2L, 'sequence': 'CTAAGGCCTACAGCCTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCAGATTTTCCTGGAATCC'}, {'score': 3, 'version': 2L, 'sequence': 'GCCAGATTTTCCTGGAATCC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 99, 'version': 0L, 'sequence': 'TAGGGTCCATCTTAACC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGGGTCCATCTTAACC'})
({'score': 99, 'version': 0L, 'sequence': 'TTAAACCAAACAGCCCCACC'}, {'score': 100, 'version': 2L, 'sequence': 'TTAAACCAAACAGCCCCACC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCAGACTGAGGGCCCCC'}, {'score': 96, 'version': 2L, 'sequence': 'AGTGCAGACTGAGGGCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCACTGGCTGGAAGGTTTC'}, {'score': 98, 'version': 2L, 'sequence': 'GGCACTGGCTGGAAGGTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGGCCCCCCACCCCAGTC'}, {'score': 45, 'version': 2L, 'sequence': 'TAGGGCCCCCCACCCCAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTGGGCTTTTAGGGGTATC'}, {'score': 3, 'version': 2L, 'sequence': 'GCTGGGCTTTTAGGGGTATC'})
({'score': 100, 'version': 0L, 'sequence': 'GTAATGCCTATTGGTTCTTC'}, {'score': 86, 'version': 2L, 'sequence': 'GTAATGCCTATTGGTTCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTCTCCTACATACCCCAGC'}, {'score': 38, 'version': 2L, 'sequence': 'GTTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCCACCTTC'}, {'score': 18, 'version': 2L, 'sequence': 'TCCTACCCTAACCCACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTTACAAACCTTC'}, {'score': 33, 'version': 2L, 'sequence': 'TCCTACCCTTACAAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTTACAAACCTTC'}, {'score': 33, 'version': 2L, 'sequence': 'TCCTACCCTTACAAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTTACAAACCTTC'}, {'score': 33, 'version': 2L, 'sequence': 'TCCTACCCTTACAAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTAACCAACCTTC'}, {'score': 8, 'version': 2L, 'sequence': 'TCCTACCCTAACCAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTTACAAACCTTC'}, {'score': 33, 'version': 2L, 'sequence': 'TCCTACCCTTACAAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTACCCTTACAAACCTTC'}, {'score': 33, 'version': 2L, 'sequence': 'TCCTACCCTTACAAACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTCTCCTACATACCCCAGC'}, {'score': 6, 'version': 2L, 'sequence': 'GCTCTCCTACATACCCCAGC'})
({'score': 95, 'version': 0L, 'sequence': 'AAATCAGACAACCACCGAAC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATCAGACAACCACCGAAC'})
({'score': 43, 'version': 0L, 'sequence': 'ACAGGCAGCAGTGCTGTAAC'}, {'score': 62, 'version': 2L, 'sequence': 'ACAGGCAGCAGTGCTGTAAC'})
({'score': 94, 'version': 0L, 'sequence': 'AACAATGTGTGATATTAAAC'}, {'score': 93, 'version': 2L, 'sequence': 'AACAATGTGTGATATTAAAC'})
({'score': 99, 'version': 0L, 'sequence': 'CTGAAATCTTTAGACTAATC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGAAATCTTTAGACTAATC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTTCCAGTCATGTGATTC'}, {'score': 11, 'version': 2L, 'sequence': 'TTCTTCCAGTCATGTGATTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAAAATATTGATTGTTTTC'}, {'score': 98, 'version': 2L, 'sequence': 'ATAAAATATTGATTGTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CACCCCCCAGCTGGCAACCC'}, {'score': 95, 'version': 2L, 'sequence': 'CACCCCCCAGCTGGCAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'CAAACACACACAAATATATC'}, {'score': 96, 'version': 2L, 'sequence': 'CAAACACACACAAATATATC'})
({'score': 99, 'version': 0L, 'sequence': 'TCTCCCTATGGGTGCTGACC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTCCCTATGGGTGCTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'TATCACAGGAGCTCCCCATC'}, {'score': 35, 'version': 2L, 'sequence': 'TATCACAGGAGCTCCCCATC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGGGTCGCAGACACTTTCC'}, {'score': 24, 'version': 2L, 'sequence': 'AAGGGTCGCAGACACTTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGACTGTCTCTCTGTCTGTC'}, {'score': 95, 'version': 2L, 'sequence': 'AGACTGTCTCTCTGTCTGTC'})
({'score': 99, 'version': 0L, 'sequence': 'TTGTGTGGCCATTATAAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGTGTGGCCATTATAAAGC'})
({'score': 30, 'version': 0L, 'sequence': 'TGTATCAGCCTGTGTATGGC'}, {'score': 50, 'version': 2L, 'sequence': 'TGTATCAGCCTGTGTATGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTAAGCTGCCAAGTTGGTC'}, {'score': 74, 'version': 2L, 'sequence': 'TGTAAGCTGCCAAGTTGGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGCCCAGGACCAAAGCTTC'}, {'score': 99, 'version': 2L, 'sequence': 'CTGCCCAGGACCAAAGCTTC'})
({'score': 89, 'version': 0L, 'sequence': 'CATACACATGAAAACAAAAC'}, {'score': 98, 'version': 2L, 'sequence': 'CATACACATGAAAACAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTCATCTTTATTGACCTTC'}, {'score': 22, 'version': 2L, 'sequence': 'TCTCATCTTTATTGACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGTTTATTTGGTTTACCCC'}, {'score': 90, 'version': 2L, 'sequence': 'GTGTTTATTTGGTTTACCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCCACTGGTTGCCATACTC'}, {'score': 98, 'version': 2L, 'sequence': 'GCCCACTGGTTGCCATACTC'})
({'score': 97, 'version': 0L, 'sequence': 'GTACTGCCTTTATCCCACAC'}, {'score': 100, 'version': 2L, 'sequence': 'GTACTGCCTTTATCCCACAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCCACAAGTTGTTTGTTAC'}, {'score': 93, 'version': 2L, 'sequence': 'AGCCACAAGTTGTTTGTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGTATTTGGTGTGACCCCC'}, {'score': 32, 'version': 2L, 'sequence': 'GAGTATTTGGTGTGACCCCC'})
({'score': 90, 'version': 0L, 'sequence': 'ACATTTAGTTATTGCTGCAC'}, {'score': 88, 'version': 2L, 'sequence': 'ACATTTAGTTATTGCTGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTTGATGGTCAACCACTTC'}, {'score': 3, 'version': 2L, 'sequence': 'CTTTGATGGTCAACCACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGAAATTCAAGACCCTGCC'}, {'score': 64, 'version': 2L, 'sequence': 'AGGAAATTCAAGACCCTGCC'})
({'score': 89, 'version': 0L, 'sequence': 'TTTAGTTTCGTTTGTTTTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTAGTTTCGTTTGTTTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATTGGCATTCGGCTAAGC'}, {'score': 98, 'version': 2L, 'sequence': 'ATATTGGCATTCGGCTAAGC'})
({'score': 99, 'version': 0L, 'sequence': 'CTCCCTGCCTTGTGCCCCTC'}, {'score': 97, 'version': 2L, 'sequence': 'CTCCCTGCCTTGTGCCCCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAATAGACAGCACCCCCCC'}, {'score': 68, 'version': 2L, 'sequence': 'CCAATAGACAGCACCCCCCC'})
({'score': 97, 'version': 0L, 'sequence': 'ACTGATTTTATTGTCATTGC'}, {'score': 96, 'version': 2L, 'sequence': 'ACTGATTTTATTGTCATTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGAATCAAACCAAGGGTTC'}, {'score': 62, 'version': 2L, 'sequence': 'TGGAATCAAACCAAGGGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTTGTTGCTCACAACAATC'}, {'score': 96, 'version': 2L, 'sequence': 'ATTTGTTGCTCACAACAATC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTCCTATCTTTCCATACAC'}, {'score': 99, 'version': 2L, 'sequence': 'TCTCCTATCTTTCCATACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCATGAAAGTGGAGTTTGCC'}, {'score': 96, 'version': 2L, 'sequence': 'GCATGAAAGTGGAGTTTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAGGCCATGTGTTTAAGTC'}, {'score': 99, 'version': 2L, 'sequence': 'ATAGGCCATGTGTTTAAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGAGATGTGCAGTGACATC'}, {'score': 2, 'version': 2L, 'sequence': 'GAGAGATGTGCAGTGACATC'})
({'score': 98, 'version': 0L, 'sequence': 'AGAAGGAAAGGTGCAACCAC'}, {'score': 17, 'version': 2L, 'sequence': 'AGAAGGAAAGGTGCAACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTGAAAAAGTAGCTGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CCTGAAAAAGTAGCTGCTAC'})
({'score': 0, 'version': 0L, 'sequence': 'CAAATTGAGGAATATTGGCC'}, {'score': 87, 'version': 2L, 'sequence': 'CAAATTGAGGAATATTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCATTTCAGCAGCTGGTTGC'}, {'score': 9, 'version': 2L, 'sequence': 'GCATTTCAGCAGCTGGTTGC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'ACAAAGGCAGATGCCAGACC'}, {'score': 0, 'version': 2L, 'sequence': 'ACAAAGGCAGATGCCAGACC'})
({'score': 12, 'version': 0L, 'sequence': 'ATTCCTTATCTGGAAAACCC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTCCTTATCTGGAAAACCC'})
({'score': 98, 'version': 0L, 'sequence': 'TATGTAAAAAGTGAGAAGAC'}, {'score': 70, 'version': 2L, 'sequence': 'TATGTAAAAAGTGAGAAGAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGGGGTAATTTACCATTAC'}, {'score': 93, 'version': 2L, 'sequence': 'GGGGGGTAATTTACCATTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTCATTTCTTATGTCACCC'}, {'score': 98, 'version': 2L, 'sequence': 'TGTCATTTCTTATGTCACCC'})
({'score': 100, 'version': 0L, 'sequence': 'CATTCATTGGTTGATGGGCC'}, {'score': 36, 'version': 2L, 'sequence': 'CATTCATTGGTTGATGGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCAAGGCACCTATACATGC'}, {'score': 2, 'version': 2L, 'sequence': 'GCCAAGGCACCTATACATGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAATAGTAAAAAATGTCCGC'}, {'score': 99, 'version': 2L, 'sequence': 'AAATAGTAAAAAATGTCCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GACTATGGTGTTTTTTAGCC'}, {'score': 4, 'version': 2L, 'sequence': 'GACTATGGTGTTTTTTAGCC'})
({'score': 99, 'version': 0L, 'sequence': 'GACCGACAGCTGGAATTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'GACCGACAGCTGGAATTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAAATTGTAAGCACAGAGC'}, {'score': 90, 'version': 2L, 'sequence': 'AAAAATTGTAAGCACAGAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CACTGGCAGCGAAAAAAAAC'}, {'score': 96, 'version': 2L, 'sequence': 'CACTGGCAGCGAAAAAAAAC'})
({'score': 62, 'version': 0L, 'sequence': 'TTGCCCGGAATAGGATGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGCCCGGAATAGGATGTTC'})
({'score': 18, 'version': 0L, 'sequence': 'GTTTAGCTTTTAGTATGATC'}, {'score': 23, 'version': 2L, 'sequence': 'GTTTAGCTTTTAGTATGATC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTATTGGGCCATGTATAGC'}, {'score': 99, 'version': 2L, 'sequence': 'TTTATTGGGCCATGTATAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTATTTTACATGTATATTCC'}, {'score': 97, 'version': 2L, 'sequence': 'TTATTTTACATGTATATTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TATCTTTTTAATGGGTGGGC'}, {'score': 99, 'version': 2L, 'sequence': 'TATCTTTTTAATGGGTGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TACTGTCCCTTCACCTTCTC'}, {'score': 64, 'version': 2L, 'sequence': 'TACTGTCCCTTCACCTTCTC'})
({'score': 44, 'version': 0L, 'sequence': 'TTTTACATACAAGCAAACAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTTACATACAAGCAAACAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTATGTTTGGGCTTCTCTAC'}, {'score': 36, 'version': 2L, 'sequence': 'TTATGTTTGGGCTTCTCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGCACATGCTCAGTGTGTC'}, {'score': 24, 'version': 2L, 'sequence': 'CTGCACATGCTCAGTGTGTC'})
({'score': 100, 'version': 0L, 'sequence': 'AATCCAAAAACTATCATATC'}, {'score': 94, 'version': 2L, 'sequence': 'AATCCAAAAACTATCATATC'})
({'score': 82, 'version': 0L, 'sequence': 'TGGGTTGGGAGAGGGTGGAC'}, {'score': 90, 'version': 2L, 'sequence': 'TGGGTTGGGAGAGGGTGGAC'})
({'score': 100, 'version': 0L, 'sequence': 'CATACCTGTACATACATTTC'}, {'score': 85, 'version': 2L, 'sequence': 'CATACCTGTACATACATTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CAAATAATATTAAAGACACC'}, {'score': 90, 'version': 2L, 'sequence': 'CAAATAATATTAAAGACACC'})
({'score': 78, 'version': 0L, 'sequence': 'AATTTTCCAATGGGCAGAAC'}, {'score': 97, 'version': 2L, 'sequence': 'AATTTTCCAATGGGCAGAAC'})
({'score': 98, 'version': 0L, 'sequence': 'GTGTTCTGAGGAGGGGTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'GTGTTCTGAGGAGGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTTCTTCAACTCCACCTTC'}, {'score': 94, 'version': 2L, 'sequence': 'CCTTCTTCAACTCCACCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAACTGGAGGTCCAGGGAC'}, {'score': 50, 'version': 2L, 'sequence': 'CCAACTGGAGGTCCAGGGAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTTGGGGTTGTTTTGCGAC'}, {'score': 98, 'version': 2L, 'sequence': 'GCTTGGGGTTGTTTTGCGAC'})
({'score': 62, 'version': 0L, 'sequence': 'CCAAATCCTACAGAAAGTAC'}, {'score': 96, 'version': 2L, 'sequence': 'CCAAATCCTACAGAAAGTAC'})
({'score': 47, 'version': 0L, 'sequence': 'CTAAAGATGGTAGAATTTTC'}, {'score': 95, 'version': 2L, 'sequence': 'CTAAAGATGGTAGAATTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGACATTTAAAAAATAATTC'}, {'score': 81, 'version': 2L, 'sequence': 'TGACATTTAAAAAATAATTC'})
({'score': 99, 'version': 0L, 'sequence': 'CCTCTGCAGGGGGGGCCATC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTCTGCAGGGGGGGCCATC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCCTTTTTTTTTTATTGCC'}, {'score': 92, 'version': 2L, 'sequence': 'TCCCTTTTTTTTTTATTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CAACTAAAGGGGTAAATGTC'}, {'score': 98, 'version': 2L, 'sequence': 'CAACTAAAGGGGTAAATGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GGAATCTGAATTGCTTTATC'}, {'score': 96, 'version': 2L, 'sequence': 'GGAATCTGAATTGCTTTATC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAATGGTATAATATATATC'}, {'score': 99, 'version': 2L, 'sequence': 'GAAATGGTATAATATATATC'})
({'score': 100, 'version': 0L, 'sequence': 'CGCACACACAGCCACACCCC'}, {'score': 91, 'version': 2L, 'sequence': 'CGCACACACAGCCACACCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCATTGCAAAATTGCCATC'}, {'score': 89, 'version': 2L, 'sequence': 'TGCATTGCAAAATTGCCATC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCCCTGGGTTTCCCTGTGC'}, {'score': 25, 'version': 2L, 'sequence': 'CTCCCTGGGTTTCCCTGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'ACAGTATTCAAAATCTTGTC'}, {'score': 97, 'version': 2L, 'sequence': 'ACAGTATTCAAAATCTTGTC'})
({'score': 98, 'version': 0L, 'sequence': 'CACTGATTTCCAGCAAAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'CACTGATTTCCAGCAAAAGC'})
({'score': 50, 'version': 0L, 'sequence': 'TAGACTTTTGTGGTTATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGACTTTTGTGGTTATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATCACTGGCTAAACATATGC'}, {'score': 99, 'version': 2L, 'sequence': 'ATCACTGGCTAAACATATGC'})
({'score': 100, 'version': 0L, 'sequence': 'TATGCTTACGTACCAATGAC'}, {'score': 98, 'version': 2L, 'sequence': 'TATGCTTACGTACCAATGAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATCAAATATAAATTACAC'}, {'score': 98, 'version': 2L, 'sequence': 'ATATCAAATATAAATTACAC'})
({'score': 87, 'version': 0L, 'sequence': 'TAGGTACTGTGGGGCTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGGTACTGTGGGGCTGCAC'})
({'score': 99, 'version': 0L, 'sequence': 'TGGCAAGCCGTAGCCCTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGCAAGCCGTAGCCCTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGAACATGTAAGTCGC'}, {'score': 93, 'version': 2L, 'sequence': 'GCGAACATGTAAGTCGC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCTTCAGGACCTGGACGGC'}, {'score': 50, 'version': 2L, 'sequence': 'GGCTTCAGGACCTGGACGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTTTTTATTCAACCAAAAC'}, {'score': 99, 'version': 2L, 'sequence': 'TGTTTTTATTCAACCAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGTAGTCCACCAATAGCC'}, {'score': 50, 'version': 2L, 'sequence': 'TTTGTAGTCCACCAATAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTTTCTGTCCTTAGAGTTC'}, {'score': 98, 'version': 2L, 'sequence': 'GTTTTCTGTCCTTAGAGTTC'})
({'score': 47, 'version': 0L, 'sequence': 'CCTTGAATCAGGGCCAGAAC'}, {'score': 91, 'version': 2L, 'sequence': 'CCTTGAATCAGGGCCAGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GATATGGCAGCTTCTATTTC'}, {'score': 97, 'version': 2L, 'sequence': 'GATATGGCAGCTTCTATTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAGATGGTAAATCTCCAGC'}, {'score': 24, 'version': 2L, 'sequence': 'CCAGATGGTAAATCTCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CAAATTTGTAATTCTGGCCC'}, {'score': 3, 'version': 2L, 'sequence': 'CAAATTTGTAATTCTGGCCC'})
({'score': 97, 'version': 0L, 'sequence': 'GACATAAGGGAAGTTGCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GACATAAGGGAAGTTGCCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAGTTTATTTTAAATTTCC'}, {'score': 85, 'version': 2L, 'sequence': 'CCAGTTTATTTTAAATTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTTGGTTACATTGAACCC'}, {'score': 24, 'version': 2L, 'sequence': 'TGCTTGGTTACATTGAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTGTTATTGACAATAAAC'}, {'score': 98, 'version': 2L, 'sequence': 'CTCTGTTATTGACAATAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TATTTAATTATTTACAATGC'}, {'score': 58, 'version': 2L, 'sequence': 'TATTTAATTATTTACAATGC'})
({'score': 100, 'version': 0L, 'sequence': 'CGTCACAGATGCGAGCTCAC'}, {'score': 95, 'version': 2L, 'sequence': 'CGTCACAGATGCGAGCTCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCCTGATCTGTGTATAATC'}, {'score': 91, 'version': 2L, 'sequence': 'TCCCTGATCTGTGTATAATC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGTTTACATTATTTATAC'}, {'score': 95, 'version': 2L, 'sequence': 'ATTGTTTACATTATTTATAC'})
({'score': 100, 'version': 0L, 'sequence': 'GACTCGGGCTGTGGGATAAC'}, {'score': 0, 'version': 2L, 'sequence': 'GACTCGGGCTGTGGGATAAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACATGTCTCCCCTACAAAAC'}, {'score': 95, 'version': 2L, 'sequence': 'ACATGTCTCCCCTACAAAAC'})
({'score': 36, 'version': 0L, 'sequence': 'GGGAAATCGCCGGACAAGAC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGAAATCGCCGGACAAGAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTTAACTACAGAAATAGGC'}, {'score': 1, 'version': 2L, 'sequence': 'GCTTAACTACAGAAATAGGC'})
({'score': 100, 'version': 0L, 'sequence': 'AACATAATGAACAAAATTTC'}, {'score': 9, 'version': 2L, 'sequence': 'AACATAATGAACAAAATTTC'})
({'score': 69, 'version': 0L, 'sequence': 'GTATATGGTCTGGGCTGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTATATGGTCTGGGCTGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTCCCTAAGCTCAGGTAAC'}, {'score': 8, 'version': 2L, 'sequence': 'AGTCCCTAAGCTCAGGTAAC'})
({'score': 94, 'version': 0L, 'sequence': 'TATATAAAATATGGCATTCC'}, {'score': 6, 'version': 2L, 'sequence': 'TATATAAAATATGGCATTCC'})
({'score': 96, 'version': 0L, 'sequence': 'GTTACTGGGACAGAAGACAC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTACTGGGACAGAAGACAC'})
({'score': 86, 'version': 0L, 'sequence': 'GTAGTTAAAATAGGAACTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTAGTTAAAATAGGAACTCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACAAAGGCAGATGCCAGACC'}, {'score': 0, 'version': 2L, 'sequence': 'ACAAAGGCAGATGCCAGACC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 22, 'version': 0L, 'sequence': 'TTCTTCAGCTATCTTGTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCTTCAGCTATCTTGTTGC'})
({'score': 95, 'version': 0L, 'sequence': 'TGAAGAAATTGGAAACTGAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGAAGAAATTGGAAACTGAC'})
({'score': 95, 'version': 0L, 'sequence': 'GGGGGTGGAAATGTGTTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGGGTGGAAATGTGTTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CGTCTCCCCTCTGGGTAGGC'}, {'score': 97, 'version': 2L, 'sequence': 'CGTCTCCCCTCTGGGTAGGC'})
({'score': 100, 'version': 0L, 'sequence': 'ACAAGATTTATGCTGTAGGC'}, {'score': 65, 'version': 2L, 'sequence': 'ACAAGATTTATGCTGTAGGC'})
({'score': 97, 'version': 0L, 'sequence': 'CCCTATTCAGCTTTCTATTC'}, {'score': 100, 'version': 2L, 'sequence': 'CCCTATTCAGCTTTCTATTC'})
({'score': 97, 'version': 0L, 'sequence': 'GGCATTTCCTAATGCAGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGCATTTCCTAATGCAGAGC'})
({'score': 79, 'version': 0L, 'sequence': 'ACGAGGGGGGGGGGGGTGGC'}, {'score': 100, 'version': 2L, 'sequence': 'ACGAGGGGGGGGGGGGTGGC'})
({'score': 98, 'version': 0L, 'sequence': 'ATTTAATCAACCCTTTCCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATTTAATCAACCCTTTCCAC'})
({'score': 61, 'version': 0L, 'sequence': 'TTTGTTTAGGAAACAGAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTGTTTAGGAAACAGAAGC'})
({'score': 94, 'version': 0L, 'sequence': 'CAAGCATCAACATATTATTC'}, {'score': 100, 'version': 2L, 'sequence': 'CAAGCATCAACATATTATTC'})
({'score': 35, 'version': 0L, 'sequence': 'CCTCAACCCCTTTATTTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTCAACCCCTTTATTTTCC'})
({'score': 20, 'version': 0L, 'sequence': 'ATTATGGATAGAAAGGAGAC'}, {'score': 17, 'version': 2L, 'sequence': 'ATTATGGATAGAAAGGAGAC'})
({'score': 0, 'version': 0L, 'sequence': 'TCCGGGGGCGGTGAGATCGC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCGGGGGCGGTGAGATCGC'})
({'score': 0, 'version': 0L, 'sequence': 'CGCAATTGTGCTCTCTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'CGCAATTGTGCTCTCTGCAC'})
({'score': 0, 'version': 0L, 'sequence': 'CACAATTGCGCTCTCTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'CACAATTGCGCTCTCTGCAC'})
({'score': 6, 'version': 0L, 'sequence': 'AACCGAAAATTTGGCTCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AACCGAAAATTTGGCTCTTC'})
({'score': 9, 'version': 0L, 'sequence': 'TGTGGCATTTAATCCGCCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTGGCATTTAATCCGCCAC'})
({'score': 5, 'version': 0L, 'sequence': 'CGGATAATATGCTCTTTGTC'}, {'score': 100, 'version': 2L, 'sequence': 'CGGATAATATGCTCTTTGTC'})
({'score': 59, 'version': 0L, 'sequence': 'ATGTCCGGACTGGGCTTCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATGTCCGGACTGGGCTTCAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTAGGGTTGCCACCTCTCC'}, {'score': 15, 'version': 2L, 'sequence': 'ACTAGGGTTGCCACCTCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTAGGGTTGCCACCTCTCC'}, {'score': 15, 'version': 2L, 'sequence': 'ACTAGGGTTGCCACCTCTCC'})
({'score': 3, 'version': 0L, 'sequence': 'GCCGGGAGAGGTGGCAACCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCGGGAGAGGTGGCAACCC'})
({'score': 3, 'version': 0L, 'sequence': 'GCCGGGAGAGGTGGCAACCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCGGGAGAGGTGGCAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTAGGGTTGCCACCTCTCC'}, {'score': 15, 'version': 2L, 'sequence': 'ACTAGGGTTGCCACCTCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTAGGGTTGCCACCTCTCC'}, {'score': 15, 'version': 2L, 'sequence': 'ACTAGGGTTGCCACCTCTCC'})
({'score': 59, 'version': 0L, 'sequence': 'ATGTCCAGACTGGGCTTCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATGTCCAGACTGGGCTTCAC'})
({'score': 49, 'version': 0L, 'sequence': 'TCAACAAGCCAATGTGGTTC'}, {'score': 99, 'version': 2L, 'sequence': 'TCAACAAGCCAATGTGGTTC'})
({'score': 95, 'version': 0L, 'sequence': 'TTTGCTTTTTTTGTGGAATC'}, {'score': 99, 'version': 2L, 'sequence': 'TTTGCTTTTTTTGTGGAATC'})
({'score': 99, 'version': 0L, 'sequence': 'GCATGTCTAAAGTGTCTATC'}, {'score': 100, 'version': 2L, 'sequence': 'GCATGTCTAAAGTGTCTATC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTAGATTGTAAGCTCAC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCTTAGATTGTAAGCTCAC'})
({'score': 91, 'version': 0L, 'sequence': 'TGCAATGGTTATTCTATGGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCAATGGTTATTCTATGGC'})
({'score': 98, 'version': 0L, 'sequence': 'CCTCACTCTCCTTCCTCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTCACTCTCCTTCCTCCTC'})
({'score': 99, 'version': 0L, 'sequence': 'TACAGTAGGGTTTCTTAACC'}, {'score': 100, 'version': 2L, 'sequence': 'TACAGTAGGGTTTCTTAACC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCCTGTACACTGATAGGGC'}, {'score': 94, 'version': 2L, 'sequence': 'CTCCTGTACACTGATAGGGC'})
({'score': 1, 'version': 0L, 'sequence': 'TAAACAGTAATTAAACCAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TAAACAGTAATTAAACCAAC'})
({'score': 17, 'version': 0L, 'sequence': 'TATTGGAGCCAAAACAATCC'}, {'score': 99, 'version': 2L, 'sequence': 'TATTGGAGCCAAAACAATCC'})
({'score': 28, 'version': 0L, 'sequence': 'GTTTGCCCAGAAGTAGTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTTGCCCAGAAGTAGTAAC'})
({'score': 39, 'version': 0L, 'sequence': 'GAGCATTAAACCATTGCAAC'}, {'score': 100, 'version': 2L, 'sequence': 'GAGCATTAAACCATTGCAAC'})
({'score': 0, 'version': 0L, 'sequence': 'CCGTGACAAGTAACTGCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'CCGTGACAAGTAACTGCTAC'})
({'score': 91, 'version': 0L, 'sequence': 'AACAATTTTTTTTTCATCTC'}, {'score': 100, 'version': 2L, 'sequence': 'AACAATTTTTTTTTCATCTC'})
({'score': 97, 'version': 0L, 'sequence': 'TATTTATTGTTAGTACTGAC'}, {'score': 100, 'version': 2L, 'sequence': 'TATTTATTGTTAGTACTGAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGAAAAGTACACTCTGGGCC'}, {'score': 99, 'version': 2L, 'sequence': 'AGAAAAGTACACTCTGGGCC'})
({'score': 24, 'version': 0L, 'sequence': 'TTTAAGGGCTCAGTCCCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTAAGGGCTCAGTCCCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'CACTGAGCCCAAATTCAGAC'}, {'score': 95, 'version': 2L, 'sequence': 'CACTGAGCCCAAATTCAGAC'})
({'score': 100, 'version': 0L, 'sequence': 'AATATGAAGATAGACAAGGC'}, {'score': 93, 'version': 2L, 'sequence': 'AATATGAAGATAGACAAGGC'})
({'score': 96, 'version': 0L, 'sequence': 'AAAAAAGCAAATGGTTTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAAAAGCAAATGGTTTCTC'})
({'score': 99, 'version': 0L, 'sequence': 'TATATAAGGAGGGAATACAC'}, {'score': 100, 'version': 2L, 'sequence': 'TATATAAGGAGGGAATACAC'})
({'score': 97, 'version': 0L, 'sequence': 'CAATTAGCTGCTATGGGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CAATTAGCTGCTATGGGTTC'})
({'score': 96, 'version': 0L, 'sequence': 'AAATACATCATGCTCAACCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATACATCATGCTCAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATGGATAATTATGAGCAGCC'}, {'score': 78, 'version': 2L, 'sequence': 'ATGGATAATTATGAGCAGCC'})
({'score': 93, 'version': 0L, 'sequence': 'TATCCTAATGGAAATGGATC'}, {'score': 100, 'version': 2L, 'sequence': 'TATCCTAATGGAAATGGATC'})
({'score': 97, 'version': 0L, 'sequence': 'GTTGCAGGAATTCAATTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTGCAGGAATTCAATTTTC'})
({'score': 99, 'version': 0L, 'sequence': 'GAGGTATGGGTCAGGTCATC'}, {'score': 100, 'version': 2L, 'sequence': 'GAGGTATGGGTCAGGTCATC'})
({'score': 95, 'version': 0L, 'sequence': 'TCCTGGACTGCAAAAATATC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCTGGACTGCAAAAATATC'})
({'score': 67, 'version': 0L, 'sequence': 'ACTAATAAACCGTATTTTGC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTAATAAACCGTATTTTGC'})
({'score': 98, 'version': 0L, 'sequence': 'AAACATTGCAGATTGTGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAACATTGCAGATTGTGTTC'})
({'score': 98, 'version': 0L, 'sequence': 'TGTAAATTCTGTACAGCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTAAATTCTGTACAGCCTC'})
({'score': 91, 'version': 0L, 'sequence': 'GAATTATTATTTTTTTTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GAATTATTATTTTTTTTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAACTATTAAAGGGCAGTC'}, {'score': 94, 'version': 2L, 'sequence': 'CTAACTATTAAAGGGCAGTC'})
({'score': 98, 'version': 0L, 'sequence': 'GGTATGGGGAAAGTGTTGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTATGGGGAAAGTGTTGGC'})
({'score': 90, 'version': 0L, 'sequence': 'TAGCACAATTAAATTGGAAC'}, {'score': 99, 'version': 2L, 'sequence': 'TAGCACAATTAAATTGGAAC'})
({'score': 99, 'version': 0L, 'sequence': 'ATAACTTTTTCTTAAACAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATAACTTTTTCTTAAACAAC'})
({'score': 99, 'version': 0L, 'sequence': 'ATTCTACTTTATTATAATTC'}, {'score': 100, 'version': 2L, 'sequence': 'ATTCTACTTTATTATAATTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAACTACAGGATATAAATC'}, {'score': 99, 'version': 2L, 'sequence': 'GAACTACAGGATATAAATC'})
({'score': 91, 'version': 0L, 'sequence': 'TAACCAAAGTTTTTTTCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'TAACCAAAGTTTTTTTCTCC'})
({'score': 96, 'version': 0L, 'sequence': 'AAAGCAAATGAGTTGCCACC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAGCAAATGAGTTGCCACC'})
({'score': 86, 'version': 0L, 'sequence': 'TACTCAATGTTGTAAAATAC'}, {'score': 100, 'version': 2L, 'sequence': 'TACTCAATGTTGTAAAATAC'})
({'score': 93, 'version': 0L, 'sequence': 'TACCTTTAACATATGAATGC'}, {'score': 100, 'version': 2L, 'sequence': 'TACCTTTAACATATGAATGC'})
({'score': 1, 'version': 0L, 'sequence': 'CACACTATGTGTAAATACGC'}, {'score': 100, 'version': 2L, 'sequence': 'CACACTATGTGTAAATACGC'})
({'score': 15, 'version': 0L, 'sequence': 'GTCCGCGGTAATGCGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTCCGCGGTAATGCGTTTTC'})
({'score': 95, 'version': 0L, 'sequence': 'TTGACACAGTCGTTAGTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGACACAGTCGTTAGTGCC'})
({'score': 96, 'version': 0L, 'sequence': 'CTTTATGATGCCCTCACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTTTATGATGCCCTCACTTC'})
({'score': 70, 'version': 0L, 'sequence': 'ATAAGGGATAATGTACCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'ATAAGGGATAATGTACCCCC'})
({'score': 81, 'version': 0L, 'sequence': 'ATTATCCTTATAAGTTACAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATTATCCTTATAAGTTACAC'})
({'score': 59, 'version': 0L, 'sequence': 'CCCCCACCAGTGTGTGTCAC'}, {'score': 100, 'version': 2L, 'sequence': 'CCCCCACCAGTGTGTGTCAC'})
({'score': 66, 'version': 0L, 'sequence': 'AAAAATGCCTCCCGCTCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAAATGCCTCCCGCTCTCC'})
({'score': 66, 'version': 0L, 'sequence': 'CATTAACATGGAAGCCTGTC'}, {'score': 100, 'version': 2L, 'sequence': 'CATTAACATGGAAGCCTGTC'})
({'score': 66, 'version': 0L, 'sequence': 'CAGTCAGCAATCTGATTCAC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGTCAGCAATCTGATTCAC'})
({'score': 66, 'version': 0L, 'sequence': 'ATAAAATATTAACCAGTGGC'}, {'score': 100, 'version': 2L, 'sequence': 'ATAAAATATTAACCAGTGGC'})
({'score': 70, 'version': 0L, 'sequence': 'TATTTTATCCGGCCTCTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TATTTTATCCGGCCTCTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAGCACTTGCCAATCAGGC'}, {'score': 63, 'version': 2L, 'sequence': 'TCAGCACTTGCCAATCAGGC'})
({'score': 7, 'version': 0L, 'sequence': 'ATCTGGCAGGTTTAAAAATC'}, {'score': 99, 'version': 2L, 'sequence': 'ATCTGGCAGGTTTAAAAATC'})
({'score': 0, 'version': 0L, 'sequence': 'TAAACCATATATACAACCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TAAACCATATATACAACCAC'})
({'score': 42, 'version': 0L, 'sequence': 'TATTGTGATACAGTTAGTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TATTGTGATACAGTTAGTAC'})
({'score': 0, 'version': 0L, 'sequence': 'ATCTGTCATTACCCCATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCTGTCATTACCCCATCAC'})
({'score': 44, 'version': 0L, 'sequence': 'ACCTACTACCACCTCCAGAC'}, {'score': 42, 'version': 2L, 'sequence': 'ACCTACTACCACCTCCAGAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGAAGCCTGGGGCTAAAGCC'}, {'score': 96, 'version': 2L, 'sequence': 'GGAAGCCTGGGGCTAAAGCC'})
({'score': 87, 'version': 0L, 'sequence': 'GTTCATCTCCCTTTTATTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTCATCTCCCTTTTATTTC'})
({'score': 99, 'version': 0L, 'sequence': 'GGGTTGGGTGCATCCCAGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGTTGGGTGCATCCCAGCC'})
({'score': 98, 'version': 0L, 'sequence': 'TGTTCCTTTAGATATGGGTC'}, {'score': 63, 'version': 2L, 'sequence': 'TGTTCCTTTAGATATGGGTC'})
({'score': 0, 'version': 0L, 'sequence': 'TGCCAGAATCCAAAGATTGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCAGAATCCAAAGATTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTTGGAGCCACTGTGGACC'}, {'score': 99, 'version': 2L, 'sequence': 'AGTTGGAGCCACTGTGGACC'})
({'score': 99, 'version': 0L, 'sequence': 'AACCTCCCTTTATGCCAACC'}, {'score': 100, 'version': 2L, 'sequence': 'AACCTCCCTTTATGCCAACC'})
({'score': 97, 'version': 0L, 'sequence': 'CTTAAGCCAATGGCTACTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTTAAGCCAATGGCTACTCC'})
({'score': 97, 'version': 0L, 'sequence': 'AACTATAGGGTTGTCCCACC'}, {'score': 100, 'version': 2L, 'sequence': 'AACTATAGGGTTGTCCCACC'})
({'score': 0, 'version': 0L, 'sequence': 'ACCGCTGTTCCGCGGCACAC'}, {'score': 99, 'version': 2L, 'sequence': 'ACCGCTGTTCCGCGGCACAC'})
({'score': 1, 'version': 0L, 'sequence': 'GTGCCAAGAGCCACCCCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTGCCAAGAGCCACCCCCCC'})
({'score': 1, 'version': 0L, 'sequence': 'TAACAGCAGCAGTGCCCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TAACAGCAGCAGTGCCCCCC'})
({'score': 97, 'version': 0L, 'sequence': 'AAGACTTTTCTGCAGGTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGACTTTTCTGCAGGTCTC'})
({'score': 90, 'version': 0L, 'sequence': 'TCTTTGCTTTTTCTAAATTC'}, {'score': 99, 'version': 2L, 'sequence': 'TCTTTGCTTTTTCTAAATTC'})
({'score': 0, 'version': 0L, 'sequence': 'GGAAACATTTCACCCATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'GGAAACATTTCACCCATCAC'})
({'score': 99, 'version': 0L, 'sequence': 'TGGTGCTGATTTCATATATC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGTGCTGATTTCATATATC'})
({'score': 98, 'version': 0L, 'sequence': 'TATAAAACTGGAAATATATC'}, {'score': 100, 'version': 2L, 'sequence': 'TATAAAACTGGAAATATATC'})
({'score': 99, 'version': 0L, 'sequence': 'ATACATAAAGCATATAAATC'}, {'score': 100, 'version': 2L, 'sequence': 'ATACATAAAGCATATAAATC'})
({'score': 66, 'version': 0L, 'sequence': 'CATTAACATGGAAGCCTGTC'}, {'score': 100, 'version': 2L, 'sequence': 'CATTAACATGGAAGCCTGTC'})
({'score': 66, 'version': 0L, 'sequence': 'AAAAATGCCTCCCGCTCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAAATGCCTCCCGCTCTCC'})
({'score': 43, 'version': 0L, 'sequence': 'TAGATTTTTGAAGGGGTAC'}, {'score': 99, 'version': 2L, 'sequence': 'TAGATTTTTGAAGGGGTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATCAGGAATAATAGACAAAC'}, {'score': 93, 'version': 2L, 'sequence': 'ATCAGGAATAATAGACAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTTAGCGAGAGGCCAGTGC'}, {'score': 98, 'version': 2L, 'sequence': 'CCTTAGCGAGAGGCCAGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTTTTTACCTTCTTTTTC'}, {'score': 90, 'version': 2L, 'sequence': 'CTCTTTTTACCTTCTTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'AAATCAAATTATAGTAGTAC'}, {'score': 50, 'version': 2L, 'sequence': 'AAATCAAATTATAGTAGTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCCAGGCACGGGCTGATTC'}, {'score': 60, 'version': 2L, 'sequence': 'TGCCAGGCACGGGCTGATTC'})
({'score': 89, 'version': 0L, 'sequence': 'CAGGGACCGATATCGGCAGC'}, {'score': 0, 'version': 2L, 'sequence': 'CAGGGACCGATATCGGCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGTCGACCCGCACATCAC'}, {'score': 9, 'version': 2L, 'sequence': 'TTTGTCGACCCGCACATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGCATCATAGGTATCTGCC'}, {'score': 59, 'version': 2L, 'sequence': 'AGGCATCATAGGTATCTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACCCCTTCCACTCTGCACCC'}, {'score': 99, 'version': 2L, 'sequence': 'ACCCCTTCCACTCTGCACCC'})
({'score': 94, 'version': 0L, 'sequence': 'ACTAAAACCTGGCAATCTGC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTAAAACCTGGCAATCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATCTTCCACTGACTTCTC'}, {'score': 59, 'version': 2L, 'sequence': 'ATATCTTCCACTGACTTCTC'})
({'score': 96, 'version': 0L, 'sequence': 'TACATTCCAATATTCCTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TACATTCCAATATTCCTCTC'})
({'score': 95, 'version': 0L, 'sequence': 'TACAGAAGCAATAATATTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TACAGAAGCAATAATATTTC'})
({'score': 82, 'version': 0L, 'sequence': 'ACATTATAAATATAAAAAAC'}, {'score': 89, 'version': 2L, 'sequence': 'ACATTATAAATATAAAAAAC'})
({'score': 96, 'version': 0L, 'sequence': 'CCGAAATATTTTTGCTTTAC'}, {'score': 95, 'version': 2L, 'sequence': 'CCGAAATATTTTTGCTTTAC'})
({'score': 99, 'version': 0L, 'sequence': 'CTGCTAAAACCATATATGTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGCTAAAACCATATATGTC'})
({'score': 97, 'version': 0L, 'sequence': 'TGGATAGTTTAATAAATGAC'}, {'score': 8, 'version': 2L, 'sequence': 'TGGATAGTTTAATAAATGAC'})
({'score': 96, 'version': 0L, 'sequence': 'AAAAAGAACACAAAAACCTC'}, {'score': 32, 'version': 2L, 'sequence': 'AAAAAGAACACAAAAACCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGGGGTGTGCCGAGCCCAC'}, {'score': 35, 'version': 2L, 'sequence': 'CTGGGGTGTGCCGAGCCCAC'})
({'score': 99, 'version': 0L, 'sequence': 'ATGTATCAGTATCAGTAAGC'}, {'score': 96, 'version': 2L, 'sequence': 'ATGTATCAGTATCAGTAAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCCCATAATACACTTTTCC'}, {'score': 96, 'version': 2L, 'sequence': 'TCCCCATAATACACTTTTCC'})
({'score': 95, 'version': 0L, 'sequence': 'GAAAGCTATACAGTAATTAC'}, {'score': 100, 'version': 2L, 'sequence': 'GAAAGCTATACAGTAATTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTTGATGGGTTATAAACC'}, {'score': 91, 'version': 2L, 'sequence': 'TTTTTGATGGGTTATAAACC'})
({'score': 91, 'version': 0L, 'sequence': 'GCTGCCGCGTTTGCCATCCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTGCCGCGTTTGCCATCCC'})
({'score': 94, 'version': 0L, 'sequence': 'ACAAGGGCAACTAAAGCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'ACAAGGGCAACTAAAGCTTC'})
({'score': 64, 'version': 0L, 'sequence': 'GTGAAGACACGTGGCGCTAC'}, {'score': 13, 'version': 2L, 'sequence': 'GTGAAGACACGTGGCGCTAC'})
({'score': 99, 'version': 0L, 'sequence': 'ATATATTAAACAAGGCTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATATATTAAACAAGGCTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGAATCTGAATTAATTGC'}, {'score': 93, 'version': 2L, 'sequence': 'TTTGAATCTGAATTAATTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTGGCTGCTGTTGAGAAGC'}, {'score': 68, 'version': 2L, 'sequence': 'CTTGGCTGCTGTTGAGAAGC'})
({'score': 60, 'version': 0L, 'sequence': 'GGGCTGCTGAACCTCTTTGC'}, {'score': 29, 'version': 2L, 'sequence': 'GGGCTGCTGAACCTCTTTGC'})
({'score': 98, 'version': 0L, 'sequence': 'GATATGACTATGGTTATGAC'}, {'score': 100, 'version': 2L, 'sequence': 'GATATGACTATGGTTATGAC'})
({'score': 99, 'version': 0L, 'sequence': 'CCATGTTATTATCACTATCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCATGTTATTATCACTATCC'})
({'score': 99, 'version': 0L, 'sequence': 'GCTTGGCTGTCTGAGGGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTTGGCTGTCTGAGGGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTACATAACATCTTTTGTC'}, {'score': 98, 'version': 2L, 'sequence': 'TTTACATAACATCTTTTGTC'})
({'score': 99, 'version': 0L, 'sequence': 'AACGTATTATGTAATTGATC'}, {'score': 100, 'version': 2L, 'sequence': 'AACGTATTATGTAATTGATC'})
({'score': 100, 'version': 0L, 'sequence': 'TTGGCGAGGTCGAAGTCTCC'}, {'score': 66, 'version': 2L, 'sequence': 'TTGGCGAGGTCGAAGTCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGGAGTATTTACGCTCTGC'}, {'score': 16, 'version': 2L, 'sequence': 'CTGGAGTATTTACGCTCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AACAATGGGCTGTCCTGTTC'}, {'score': 14, 'version': 2L, 'sequence': 'AACAATGGGCTGTCCTGTTC'})
({'score': 95, 'version': 0L, 'sequence': 'ACATGGTACTGTTTTATTTC'}, {'score': 73, 'version': 2L, 'sequence': 'ACATGGTACTGTTTTATTTC'})
({'score': 39, 'version': 0L, 'sequence': 'ACTGATTTCCTTTTTCTCTC'}, {'score': 0, 'version': 2L, 'sequence': 'ACTGATTTCCTTTTTCTCTC'})
({'score': 86, 'version': 0L, 'sequence': 'AGGTTAGTGGAGTAACCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGGTTAGTGGAGTAACCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTGGACATCAATGATCATC'}, {'score': 62, 'version': 2L, 'sequence': 'GCTGGACATCAATGATCATC'})
({'score': 96, 'version': 0L, 'sequence': 'TACTTTCTAATATTTTTTTC'}, {'score': 54, 'version': 2L, 'sequence': 'TACTTTCTAATATTTTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TAATTAGAAAGAACTTGTCC'}, {'score': 94, 'version': 2L, 'sequence': 'TAATTAGAAAGAACTTGTCC'})
({'score': 99, 'version': 0L, 'sequence': 'GATTTTTAAAAAGCGATTAC'}, {'score': 84, 'version': 2L, 'sequence': 'GATTTTTAAAAAGCGATTAC'})
({'score': 96, 'version': 0L, 'sequence': 'TACATATATATATGCGGACC'}, {'score': 99, 'version': 2L, 'sequence': 'TACATATATATATGCGGACC'})
({'score': 98, 'version': 0L, 'sequence': 'TATATATAGTATTGTATCAC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATATAGTATTGTATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGTTGCTTGGTATTAATC'}, {'score': 95, 'version': 2L, 'sequence': 'AAAGTTGCTTGGTATTAATC'})
({'score': 100, 'version': 0L, 'sequence': 'GTATGATTTAAAGATGTCTC'}, {'score': 96, 'version': 2L, 'sequence': 'GTATGATTTAAAGATGTCTC'})
({'score': 65, 'version': 0L, 'sequence': 'GCAGTGGTTCTCAACCTTCC'}, {'score': 10, 'version': 2L, 'sequence': 'GCAGTGGTTCTCAACCTTCC'})
({'score': 98, 'version': 0L, 'sequence': 'GTATTAAAGGCTTGCGGCAC'}, {'score': 11, 'version': 2L, 'sequence': 'GTATTAAAGGCTTGCGGCAC'})
({'score': 60, 'version': 0L, 'sequence': 'CCTTTAAATATATTTATCTC'}, {'score': 56, 'version': 2L, 'sequence': 'CCTTTAAATATATTTATCTC'})
({'score': 91, 'version': 0L, 'sequence': 'TATTGTAAAAAAAAGGAAAC'}, {'score': 81, 'version': 2L, 'sequence': 'TATTGTAAAAAAAAGGAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGAAGGTTTCTGCTCATCTC'}, {'score': 97, 'version': 2L, 'sequence': 'TGAAGGTTTCTGCTCATCTC'})
({'score': 10, 'version': 0L, 'sequence': 'ACAGATTGCCAGTCCTGGCC'}, {'score': 0, 'version': 2L, 'sequence': 'ACAGATTGCCAGTCCTGGCC'})
({'score': 97, 'version': 0L, 'sequence': 'TTTTTGCAGGGGGGGGTGAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTTTGCAGGGGGGGGTGAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCACCCAAAAGCTACTGCCC'}, {'score': 57, 'version': 2L, 'sequence': 'CCACCCAAAAGCTACTGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGACTTGTGGGCCTATGCC'}, {'score': 96, 'version': 2L, 'sequence': 'GGGACTTGTGGGCCTATGCC'})
({'score': 96, 'version': 0L, 'sequence': 'TGTGGAATTTGGGGTAACAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTGGAATTTGGGGTAACAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCAGTTCTTCTCAGGTGAC'}, {'score': 99, 'version': 2L, 'sequence': 'CTCAGTTCTTCTCAGGTGAC'})
({'score': 64, 'version': 0L, 'sequence': 'TGGAGCAATAGGAGGAGTTC'}, {'score': 0, 'version': 2L, 'sequence': 'TGGAGCAATAGGAGGAGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GATAGGTCCTACACCTGCAC'}, {'score': 93, 'version': 2L, 'sequence': 'GATAGGTCCTACACCTGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTGTAATTCGGAAACTTC'}, {'score': 59, 'version': 2L, 'sequence': 'TCCTGTAATTCGGAAACTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTATAGAATGCCCCATTCC'}, {'score': 1, 'version': 2L, 'sequence': 'GTTATAGAATGCCCCATTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTGGGTCATTAGAGATCAC'}, {'score': 0, 'version': 2L, 'sequence': 'TCTGGGTCATTAGAGATCAC'})
({'score': 0, 'version': 0L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 100, 'version': 0L, 'sequence': 'GATTCTTCAAAGAAAGATTC'}, {'score': 95, 'version': 2L, 'sequence': 'GATTCTTCAAAGAAAGATTC'})
({'score': 100, 'version': 0L, 'sequence': 'GAATCTTTCTTTGAAGAATC'}, {'score': 93, 'version': 2L, 'sequence': 'GAATCTTTCTTTGAAGAATC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGAATTTAGATAAGAATC'}, {'score': 93, 'version': 2L, 'sequence': 'AAAGAATTTAGATAAGAATC'})
({'score': 100, 'version': 0L, 'sequence': 'AAATCTCTTTTCTTTTTTTC'}, {'score': 70, 'version': 2L, 'sequence': 'AAATCTCTTTTCTTTTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GATTTTATTCCTTTCTGTTC'}, {'score': 60, 'version': 2L, 'sequence': 'GATTTTATTCCTTTCTGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCGAGTAAGTAGGTTACAAC'}, {'score': 95, 'version': 2L, 'sequence': 'CCGAGTAAGTAGGTTACAAC'})
({'score': 47, 'version': 0L, 'sequence': 'TATACAAAGTATAAGTGATC'}, {'score': 100, 'version': 2L, 'sequence': 'TATACAAAGTATAAGTGATC'})
({'score': 96, 'version': 0L, 'sequence': 'ACCCTTTATCTACAGACCCC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCCTTTATCTACAGACCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CACTCAATAGGGGGCAGCAC'}, {'score': 99, 'version': 2L, 'sequence': 'CACTCAATAGGGGGCAGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCATCTATCTGCATTAATC'}, {'score': 99, 'version': 2L, 'sequence': 'TTCATCTATCTGCATTAATC'})
({'score': 96, 'version': 0L, 'sequence': 'CATAGTTGGCTGCAGTAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'CATAGTTGGCTGCAGTAAAC'})
({'score': 0, 'version': 0L, 'sequence': 'CAAATTGAGGAATATTGGCC'}, {'score': 87, 'version': 2L, 'sequence': 'CAAATTGAGGAATATTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGAATATGTATTTGCTGTTC'}, {'score': 13, 'version': 2L, 'sequence': 'TGAATATGTATTTGCTGTTC'})
({'score': 93, 'version': 0L, 'sequence': 'AGCCACTCGCCGCGGTCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCCACTCGCCGCGGTCTCC'})
({'score': 98, 'version': 0L, 'sequence': 'AGGAACATGGGGTAGAGGCC'}, {'score': 99, 'version': 2L, 'sequence': 'AGGAACATGGGGTAGAGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCTGGATTCAGTGCATCAC'}, {'score': 15, 'version': 2L, 'sequence': 'TCCTGGATTCAGTGCATCAC'})
({'score': 87, 'version': 0L, 'sequence': 'GCCAAACCGAACCCGAATCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCAAACCGAACCCGAATCC'})
({'score': 100, 'version': 0L, 'sequence': 'CATTTAACCCTTTCAAATAC'}, {'score': 97, 'version': 2L, 'sequence': 'CATTTAACCCTTTCAAATAC'})
({'score': 100, 'version': 0L, 'sequence': 'CATTTGACCCTTCCAAATCC'}, {'score': 24, 'version': 2L, 'sequence': 'CATTTGACCCTTCCAAATCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTAATTAGCATCTGCTAAC'}, {'score': 19, 'version': 2L, 'sequence': 'TCTAATTAGCATCTGCTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGACATTTATAATGCCAGAC'}, {'score': 98, 'version': 2L, 'sequence': 'TGACATTTATAATGCCAGAC'})
({'score': 86, 'version': 0L, 'sequence': 'CAGAGCAGATAGTGGGGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGAGCAGATAGTGGGGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACATGGCTACTTTGTGGC'}, {'score': 95, 'version': 2L, 'sequence': 'ACACATGGCTACTTTGTGGC'})
({'score': 99, 'version': 0L, 'sequence': 'GCCCAAAAGAGCTTACAATC'}, {'score': 40, 'version': 2L, 'sequence': 'GCCCAAAAGAGCTTACAATC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCTCCTGGCATTACTCAAC'}, {'score': 99, 'version': 2L, 'sequence': 'GGCTCCTGGCATTACTCAAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTACATCCAGTAATGTAAC'}, {'score': 90, 'version': 2L, 'sequence': 'ACTACATCCAGTAATGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGCATTTTGCTTGCTGCAC'}, {'score': 96, 'version': 2L, 'sequence': 'GCGCATTTTGCTTGCTGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAAGCGAGTGGATTTTATC'}, {'score': 99, 'version': 2L, 'sequence': 'CCAAGCGAGTGGATTTTATC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAATGGAGGTGGTTAGCTC'}, {'score': 95, 'version': 2L, 'sequence': 'TCAATGGAGGTGGTTAGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGTAGCTAATTCCATGCC'}, {'score': 96, 'version': 2L, 'sequence': 'TTTGTAGCTAATTCCATGCC'})
({'score': 94, 'version': 0L, 'sequence': 'TGGAAAAGCCACAAGGGGGC'}, {'score': 96, 'version': 2L, 'sequence': 'TGGAAAAGCCACAAGGGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGTGAAAATTCCAATATCC'}, {'score': 99, 'version': 2L, 'sequence': 'CTGTGAAAATTCCAATATCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGCCCAACTGATCTCTACC'}, {'score': 99, 'version': 2L, 'sequence': 'CTGCCCAACTGATCTCTACC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTGCCCCCCCCTGTGAGTC'}, {'score': 95, 'version': 2L, 'sequence': 'TCTGCCCCCCCCTGTGAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'TACCAGCCAGTTGGCAACCC'}, {'score': 2, 'version': 2L, 'sequence': 'TACCAGCCAGTTGGCAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAAACCGCTGCCTGATTGC'}, {'score': 1, 'version': 2L, 'sequence': 'TCAAACCGCTGCCTGATTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTGAGCAGCCGTCTGGTTGC'}, {'score': 9, 'version': 2L, 'sequence': 'TTGAGCAGCCGTCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAACTGAACCTACTGAAGC'}, {'score': 93, 'version': 2L, 'sequence': 'TCAACTGAACCTACTGAAGC'})
({'score': 98, 'version': 0L, 'sequence': 'CAGTAGCTTCAGGGTTCCCC'}, {'score': 3, 'version': 2L, 'sequence': 'CAGTAGCTTCAGGGTTCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCCCATGCCTTTATAAACC'}, {'score': 92, 'version': 2L, 'sequence': 'CCCCCATGCCTTTATAAACC'})
({'score': 99, 'version': 0L, 'sequence': 'TCCAGCAACATTGTGGTCCC'}, {'score': 94, 'version': 2L, 'sequence': 'TCCAGCAACATTGTGGTCCC'})
({'score': 95, 'version': 0L, 'sequence': 'GGCCCTGGGTGTAGGGGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGCCCTGGGTGTAGGGGGCC'})
({'score': 66, 'version': 0L, 'sequence': 'ATTCGCTGCGAATCCATGCC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTCGCTGCGAATCCATGCC'})
({'score': 67, 'version': 0L, 'sequence': 'GAGAAGACACACAGAGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'GAGAAGACACACAGAGCTAC'})
({'score': 0, 'version': 0L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTATAGGAAAAGTCGGTCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTATAGAATGCCCCATTCC'}, {'score': 1, 'version': 2L, 'sequence': 'GTTATAGAATGCCCCATTCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGAACATGTAAGTCGC'}, {'score': 93, 'version': 2L, 'sequence': 'GCGAACATGTAAGTCGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTGGTTGGGGACCCCTGCCC'}, {'score': 4, 'version': 2L, 'sequence': 'GTGGTTGGGGACCCCTGCCC'})
({'score': 92, 'version': 0L, 'sequence': 'CCACGACAAGTAGCCGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CCACGACAAGTAGCCGCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTAATTTGATCATTTGGCCC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATTTGATCATTTGGCCC'})
({'score': 0, 'version': 0L, 'sequence': 'ATCTGTCATTACCCCATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCTGTCATTACCCCATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTCCTACAAACTTGGCACC'}, {'score': 3, 'version': 2L, 'sequence': 'TTTCCTACAAACTTGGCACC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCACCGGCAATTTACATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CCCACCGGCAATTTACATTC'})
({'score': 53, 'version': 0L, 'sequence': 'TAGAGAGAAGTTATATGAC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGAGAGAAGTTATATGAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTGCTACAGGGCTGCAATC'}, {'score': 99, 'version': 2L, 'sequence': 'CTTGCTACAGGGCTGCAATC'})
({'score': 62, 'version': 0L, 'sequence': 'TCAGCCCCTAATTGTTTTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCAGCCCCTAATTGTTTTAC'})
({'score': 95, 'version': 0L, 'sequence': 'GAAACATGCTATTAAGTATC'}, {'score': 97, 'version': 2L, 'sequence': 'GAAACATGCTATTAAGTATC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTGCAATAGATAGAAGCC'}, {'score': 98, 'version': 2L, 'sequence': 'CTCTGCAATAGATAGAAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGCTGCAGAAATGCCAACC'}, {'score': 85, 'version': 2L, 'sequence': 'CAGCTGCAGAAATGCCAACC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCGTGTCAGGGGAAGACTC'}, {'score': 49, 'version': 2L, 'sequence': 'GTCGTGTCAGGGGAAGACTC'})
({'score': 59, 'version': 0L, 'sequence': 'AAGCAACAGAAGAAGACCTC'}, {'score': 99, 'version': 2L, 'sequence': 'AAGCAACAGAAGAAGACCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CATCTCAATGTGTCTGCCCC'}, {'score': 95, 'version': 2L, 'sequence': 'CATCTCAATGTGTCTGCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGGGTCTTGACAGACTCCC'}, {'score': 64, 'version': 2L, 'sequence': 'CAGGGTCTTGACAGACTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTCTTGACCGACACATCAC'}, {'score': 37, 'version': 2L, 'sequence': 'TTTCTTGACCGACACATCAC'})
({'score': 99, 'version': 0L, 'sequence': 'CCAGAGCTCCTGCCCTTCTC'}, {'score': 96, 'version': 2L, 'sequence': 'CCAGAGCTCCTGCCCTTCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCAGACTGAGAGGCACAAC'}, {'score': 95, 'version': 2L, 'sequence': 'CTCAGACTGAGAGGCACAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GACAGGGAGGAAGGACACAC'}, {'score': 98, 'version': 2L, 'sequence': 'GACAGGGAGGAAGGACACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GAAGACACTTACTTTGTACC'}, {'score': 99, 'version': 2L, 'sequence': 'GAAGACACTTACTTTGTACC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGACCGGACCCTGGATCAAC'}, {'score': 13, 'version': 2L, 'sequence': 'GGACCGGACCCTGGATCAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCCCTCCCAGCTCCCTTTC'}, {'score': 49, 'version': 2L, 'sequence': 'TCCCCTCCCAGCTCCCTTTC'})
({'score': 70, 'version': 0L, 'sequence': 'TATAAATATTTGGCAGACAC'}, {'score': 97, 'version': 2L, 'sequence': 'TATAAATATTTGGCAGACAC'})
({'score': 91, 'version': 0L, 'sequence': 'TACATATAATTAAATACAGC'}, {'score': 95, 'version': 2L, 'sequence': 'TACATATAATTAAATACAGC'})
({'score': 68, 'version': 0L, 'sequence': 'ATTTTTTGCACTTTCCATGC'}, {'score': 99, 'version': 2L, 'sequence': 'ATTTTTTGCACTTTCCATGC'})
({'score': 98, 'version': 0L, 'sequence': 'CCCCCACCCCAGTCCCACTC'}, {'score': 97, 'version': 2L, 'sequence': 'CCCCCACCCCAGTCCCACTC'})
({'score': 95, 'version': 0L, 'sequence': 'GTAACCCCCAGTGATGTCAC'}, {'score': 97, 'version': 2L, 'sequence': 'GTAACCCCCAGTGATGTCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GATGGGAACCAATCAGCTGC'}, {'score': 89, 'version': 2L, 'sequence': 'GATGGGAACCAATCAGCTGC'})
({'score': 19, 'version': 0L, 'sequence': 'GCCAATCTGGGCCTGGGTCC'}, {'score': 0, 'version': 2L, 'sequence': 'GCCAATCTGGGCCTGGGTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGTATCTGAGTGGGCCAGC'}, {'score': 97, 'version': 2L, 'sequence': 'TGGTATCTGAGTGGGCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCAGGGCAGGGGACAGGCC'}, {'score': 97, 'version': 2L, 'sequence': 'GCCAGGGCAGGGGACAGGCC'})
({'score': 97, 'version': 0L, 'sequence': 'AAGCGGACAGGTGGCAACCC'}, {'score': 13, 'version': 2L, 'sequence': 'AAGCGGACAGGTGGCAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCGGCAGAAGTGGCGACCC'}, {'score': 62, 'version': 2L, 'sequence': 'GCCGGCAGAAGTGGCGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATACCAGCCAATGTACTTGC'}, {'score': 44, 'version': 2L, 'sequence': 'ATACCAGCCAATGTACTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GAGGGTTTTTGTTTTTCCCC'}, {'score': 99, 'version': 2L, 'sequence': 'GAGGGTTTTTGTTTTTCCCC'})
({'score': 81, 'version': 0L, 'sequence': 'CTTGGAAAAGTAGCTGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTTGGAAAAGTAGCTGCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TACACATTCAGTGGCGTAAC'}, {'score': 97, 'version': 2L, 'sequence': 'TACACATTCAGTGGCGTAAC'})
({'score': 94, 'version': 0L, 'sequence': 'CTGTGGGGCCCAGTAACAAC'}, {'score': 13, 'version': 2L, 'sequence': 'CTGTGGGGCCCAGTAACAAC'})
({'score': 95, 'version': 0L, 'sequence': 'TTAAATAAACTGCCATTGTC'}, {'score': 99, 'version': 2L, 'sequence': 'TTAAATAAACTGCCATTGTC'})
({'score': 85, 'version': 0L, 'sequence': 'AATAAGAGAATGTAAAAAAC'}, {'score': 99, 'version': 2L, 'sequence': 'AATAAGAGAATGTAAAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTCCCTTTACTATTGTATC'}, {'score': 94, 'version': 2L, 'sequence': 'TTTCCCTTTACTATTGTATC'})
({'score': 4, 'version': 0L, 'sequence': 'TTCCTCCTACAGGCAACTTC'}, {'score': 12, 'version': 2L, 'sequence': 'TTCCTCCTACAGGCAACTTC'})
({'score': 74, 'version': 0L, 'sequence': 'TTGGCTTGTTGATGCCGTCC'}, {'score': 53, 'version': 2L, 'sequence': 'TTGGCTTGTTGATGCCGTCC'})
({'score': 24, 'version': 0L, 'sequence': 'AGAACCCTGAGGAACCCCAC'}, {'score': 28, 'version': 2L, 'sequence': 'AGAACCCTGAGGAACCCCAC'})
({'score': 88, 'version': 0L, 'sequence': 'AGATCTGTGGAAAGGCCACC'}, {'score': 16, 'version': 2L, 'sequence': 'AGATCTGTGGAAAGGCCACC'})
({'score': 100, 'version': 0L, 'sequence': 'ATACCATACATCAGTGTTTC'}, {'score': 98, 'version': 2L, 'sequence': 'ATACCATACATCAGTGTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTATAGTGTCAAGAATATC'}, {'score': 22, 'version': 2L, 'sequence': 'GCTATAGTGTCAAGAATATC'})
({'score': 50, 'version': 0L, 'sequence': 'TGAACACCTATTTGCTGTCC'}, {'score': 9, 'version': 2L, 'sequence': 'TGAACACCTATTTGCTGTCC'})
({'score': 95, 'version': 0L, 'sequence': 'GGTGGCCTTTCAGGTATATC'}, {'score': 100, 'version': 2L, 'sequence': 'GGTGGCCTTTCAGGTATATC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGCCACCTTCATGGTCAGC'}, {'score': 99, 'version': 2L, 'sequence': 'AGGCCACCTTCATGGTCAGC'})
({'score': 85, 'version': 0L, 'sequence': 'AAACAGAAATTTGGCTCTTC'}, {'score': 5, 'version': 2L, 'sequence': 'AAACAGAAATTTGGCTCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CACAATTGCACACTCTGCAC'}, {'score': 7, 'version': 2L, 'sequence': 'CACAATTGCACACTCTGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCAATTGTGCTCTCAATAC'}, {'score': 82, 'version': 2L, 'sequence': 'TGCAATTGTGCTCTCAATAC'})
({'score': 98, 'version': 0L, 'sequence': 'CTCTTTTTTTGGAGGTCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTCTTTTTTTGGAGGTCCTC'})
({'score': 50, 'version': 0L, 'sequence': 'TATTGATGTTGCCTTATTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TATTGATGTTGCCTTATTAC'})
({'score': 68, 'version': 0L, 'sequence': 'CCAGGGTCGGAGTGGCCCAC'}, {'score': 4, 'version': 2L, 'sequence': 'CCAGGGTCGGAGTGGCCCAC'})
({'score': 98, 'version': 0L, 'sequence': 'TCCTTTGACCCCAGTGCTAC'}, {'score': 92, 'version': 2L, 'sequence': 'TCCTTTGACCCCAGTGCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'CATTTTCCAGTGCAATACTC'}, {'score': 81, 'version': 2L, 'sequence': 'CATTTTCCAGTGCAATACTC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGAAGAGACCCTGAGCTAC'}, {'score': 12, 'version': 2L, 'sequence': 'GTGAAGAGACCCTGAGCTAC'})
({'score': 99, 'version': 0L, 'sequence': 'GTGAAGAGACCCTGAGCTAC'}, {'score': 12, 'version': 2L, 'sequence': 'GTGAAGAGACCCTGAGCTAC'})
({'score': 98, 'version': 0L, 'sequence': 'GTATGGCTGTATGGATTGTC'}, {'score': 66, 'version': 2L, 'sequence': 'GTATGGCTGTATGGATTGTC'})
({'score': 99, 'version': 0L, 'sequence': 'TGCAGGCCAGAGCCAGAGAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCAGGCCAGAGCCAGAGAC'})
({'score': 42, 'version': 0L, 'sequence': 'CTAAAGAAAAAAAACTGCAC'}, {'score': 90, 'version': 2L, 'sequence': 'CTAAAGAAAAAAAACTGCAC'})
({'score': 70, 'version': 0L, 'sequence': 'TAGGGGGCCCACCGGCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGGGGGCCCACCGGCCTC'})
({'score': 5, 'version': 0L, 'sequence': 'TATTGCAAGATGGGCACAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TATTGCAAGATGGGCACAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGGGAGGCTTAACCTCCCC'}, {'score': 0, 'version': 2L, 'sequence': 'TGGGGAGGCTTAACCTCCCC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCGGGATTCGGTGCATCCC'}, {'score': 0, 'version': 2L, 'sequence': 'TCCGGGATTCGGTGCATCCC'})
({'score': 0, 'version': 0L, 'sequence': 'CTGGGATTTAAAATAGGCCC'}, {'score': 3, 'version': 2L, 'sequence': 'CTGGGATTTAAAATAGGCCC'})
({'score': 0, 'version': 0L, 'sequence': 'GTTATAAAATGGCCTATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTATAAAATGGCCTATTCC'})
({'score': 0, 'version': 0L, 'sequence': 'ATACAGTTCTAAAGAATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATACAGTTCTAAAGAATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTTCAAAATGCCTTTCCAC'}, {'score': 2, 'version': 2L, 'sequence': 'GCTTCAAAATGCCTTTCCAC'})
({'score': 0, 'version': 0L, 'sequence': 'TTGGAATTTCAGCAGCTATC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGGAATTTCAGCAGCTATC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGGTCCAGTTTAACC'}, {'score': 99, 'version': 2L, 'sequence': 'TAGGGTCCAGTTTAACC'})
({'score': 0, 'version': 0L, 'sequence': 'ATACAGTTCTAAAGAATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATACAGTTCTAAAGAATCAC'})
({'score': 0, 'version': 0L, 'sequence': 'GTTATAAAATGGCCTATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTATAAAATGGCCTATTCC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 99, 'version': 0L, 'sequence': 'GAAAAGGTGATGTACCGTGC'}, {'score': 0, 'version': 2L, 'sequence': 'GAAAAGGTGATGTACCGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCAGGAACAGCTGCCCC'}, {'score': 0, 'version': 2L, 'sequence': 'AGTGCAGGAACAGCTGCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGCAGGAACAGCTGCCCC'}, {'score': 0, 'version': 2L, 'sequence': 'AGTGCAGGAACAGCTGCCCC'})
({'score': 67, 'version': 0L, 'sequence': 'GAGAAGACACACAGAGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'GAGAAGACACACAGAGCTAC'})
({'score': 57, 'version': 0L, 'sequence': 'TTTAACAGCTATCTGGTTGC'}, {'score': 0, 'version': 2L, 'sequence': 'TTTAACAGCTATCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACACCACTGCCTGGTTGC'}, {'score': 0, 'version': 2L, 'sequence': 'TCACACCACTGCCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTGCCCCATTCTCCCCTCC'}, {'score': 21, 'version': 2L, 'sequence': 'CCTGCCCCATTCTCCCCTCC'})
({'score': 0, 'version': 0L, 'sequence': 'CAAATTGAGGAATATTGGCC'}, {'score': 87, 'version': 2L, 'sequence': 'CAAATTGAGGAATATTGGCC'})
({'score': 66, 'version': 0L, 'sequence': 'ATTCGCTGCGAATCCATGCC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTCGCTGCGAATCCATGCC'})
({'score': 3, 'version': 0L, 'sequence': 'CAAAAAAATTTGCTCATCAC'}, {'score': 0, 'version': 2L, 'sequence': 'CAAAAAAATTTGCTCATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGGCCAGGTTAGAAAATC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTGGCCAGGTTAGAAAATC'})
({'score': 100, 'version': 0L, 'sequence': 'TTATAAATCTCGAAATTATC'}, {'score': 9, 'version': 2L, 'sequence': 'TTATAAATCTCGAAATTATC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTGAATAAATATAGTGTTC'}, {'score': 0, 'version': 2L, 'sequence': 'GCTGAATAAATATAGTGTTC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 0, 'version': 0L, 'sequence': 'GTTATAAAATGGCCTATTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTATAAAATGGCCTATTCC'})
({'score': 0, 'version': 0L, 'sequence': 'ATACAGTTCTAAAGAATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATACAGTTCTAAAGAATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATTGTTTCTGTTGCTTTC'}, {'score': 95, 'version': 2L, 'sequence': 'ATATTGTTTCTGTTGCTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGTGTCTACAGAAAAAAC'}, {'score': 95, 'version': 2L, 'sequence': 'TTTGTGTCTACAGAAAAAAC'})
({'score': 70, 'version': 0L, 'sequence': 'AAAAAATTAAAGAATAGCCC'}, {'score': 98, 'version': 2L, 'sequence': 'AAAAAATTAAAGAATAGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTGGGTTCATTAGATATTCC'}, {'score': 97, 'version': 2L, 'sequence': 'TTGGGTTCATTAGATATTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCCTTGGATCCCTCCAGAC'}, {'score': 6, 'version': 2L, 'sequence': 'TGCCTTGGATCCCTCCAGAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTTCACTTTGTAAAAATTC'}, {'score': 98, 'version': 2L, 'sequence': 'TGTTCACTTTGTAAAAATTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTAATTAAAAGTAGAACCC'}, {'score': 93, 'version': 2L, 'sequence': 'ATTAATTAAAAGTAGAACCC'})
({'score': 4, 'version': 0L, 'sequence': 'CAAACATAAAATTGGGGTTC'}, {'score': 95, 'version': 2L, 'sequence': 'CAAACATAAAATTGGGGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TATTTTTGGGGTGTATACAC'}, {'score': 79, 'version': 2L, 'sequence': 'TATTTTTGGGGTGTATACAC'})
({'score': 97, 'version': 0L, 'sequence': 'GAACCTGGCTTTAGCAGATC'}, {'score': 100, 'version': 2L, 'sequence': 'GAACCTGGCTTTAGCAGATC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTTAAAATGGAGCTTTAGC'}, {'score': 99, 'version': 2L, 'sequence': 'GTTTAAAATGGAGCTTTAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TATGTATATTTGTATTTATC'}, {'score': 3, 'version': 2L, 'sequence': 'TATGTATATTTGTATTTATC'})
({'score': 44, 'version': 0L, 'sequence': 'GATGTGTATACAAGTAGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'GATGTGTATACAAGTAGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GACAAAGGAACAGGAAAGTC'}, {'score': 99, 'version': 2L, 'sequence': 'GACAAAGGAACAGGAAAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTGAATAAGTGAAATCTGC'}, {'score': 91, 'version': 2L, 'sequence': 'GCTGAATAAGTGAAATCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTTTGTTTGTTTATATTTC'}, {'score': 95, 'version': 2L, 'sequence': 'GCTTTGTTTGTTTATATTTC'})
({'score': 98, 'version': 0L, 'sequence': 'CACCCATTATAATGAAGTGC'}, {'score': 100, 'version': 2L, 'sequence': 'CACCCATTATAATGAAGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGTTTATATAAAGAAACAC'}, {'score': 92, 'version': 2L, 'sequence': 'AAGTTTATATAAAGAAACAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCCTCATCTGTTCATCTTC'}, {'score': 16, 'version': 2L, 'sequence': 'GCCCTCATCTGTTCATCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGTGGTTTGAACAAAATAC'}, {'score': 48, 'version': 2L, 'sequence': 'CAGTGGTTTGAACAAAATAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCAGCAGCTGTTTGGTTGC'}, {'score': 4, 'version': 2L, 'sequence': 'TCCAGCAGCTGTTTGGTTGC'})
({'score': 48, 'version': 0L, 'sequence': 'GTTGCCAAGGGAAGAAGTGC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTGCCAAGGGAAGAAGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAAACACTTGCAAAATGTC'}, {'score': 99, 'version': 2L, 'sequence': 'TAAAACACTTGCAAAATGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTACAGCAGCCCCTCTGGC'}, {'score': 49, 'version': 2L, 'sequence': 'CTTACAGCAGCCCCTCTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTCTAACTTAGTAATACAC'}, {'score': 98, 'version': 2L, 'sequence': 'ATTCTAACTTAGTAATACAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCGTAAATTTTGAAATGATC'}, {'score': 36, 'version': 2L, 'sequence': 'TCGTAAATTTTGAAATGATC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATATATATATTTCATTCC'}, {'score': 53, 'version': 2L, 'sequence': 'ATATATATATATTTCATTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTCTCTTAATGTCATAGC'}, {'score': 40, 'version': 2L, 'sequence': 'TTTTCTCTTAATGTCATAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CAATGCTCTGTGCAATCTGC'}, {'score': 65, 'version': 2L, 'sequence': 'CAATGCTCTGTGCAATCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTATATATTGGTGGTGGCC'}, {'score': 66, 'version': 2L, 'sequence': 'TTTATATATTGGTGGTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCCAGCACAGTGGGAATGC'}, {'score': 66, 'version': 2L, 'sequence': 'CCCCAGCACAGTGGGAATGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGATGAAGTAACCAACCAC'}, {'score': 47, 'version': 2L, 'sequence': 'GGGATGAAGTAACCAACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGCATTCTAATTCCTTTCC'}, {'score': 64, 'version': 2L, 'sequence': 'AAGCATTCTAATTCCTTTCC'})
({'score': 85, 'version': 0L, 'sequence': 'ACCTGGGATTTCATTTGATC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCTGGGATTTCATTTGATC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTTGGGACCTGGGATTTCC'}, {'score': 6, 'version': 2L, 'sequence': 'ACTTGGGACCTGGGATTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'GATCCTTTTCAGGCCTTAGC'}, {'score': 64, 'version': 2L, 'sequence': 'GATCCTTTTCAGGCCTTAGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGGAGCAACACTGGTCAC'}, {'score': 66, 'version': 2L, 'sequence': 'AAAGGAGCAACACTGGTCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGCCTCCTCAAGCTTTTC'}, {'score': 66, 'version': 2L, 'sequence': 'TTTGCCTCCTCAAGCTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTCTGGATGTCCCGATAC'}, {'score': 66, 'version': 2L, 'sequence': 'TTCTCTGGATGTCCCGATAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATACCATTTATTATGCCCCC'}, {'score': 40, 'version': 2L, 'sequence': 'ATACCATTTATTATGCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAGGAGCTTATATTGTTCC'}, {'score': 66, 'version': 2L, 'sequence': 'TAAGGAGCTTATATTGTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTGCAGCAATAGAAAATGAC'}, {'score': 93, 'version': 2L, 'sequence': 'TTGCAGCAATAGAAAATGAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTTCCAGAAGATAATAAC'}, {'score': 66, 'version': 2L, 'sequence': 'TGCTTCCAGAAGATAATAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CATGGAAACATGTGAGCAAC'}, {'score': 52, 'version': 2L, 'sequence': 'CATGGAAACATGTGAGCAAC'})
({'score': 64, 'version': 0L, 'sequence': 'CATTGCCCCTCTTTGAGTCC'}, {'score': 66, 'version': 2L, 'sequence': 'CATTGCCCCTCTTTGAGTCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTCAAAGAGGGGCAATGGC'}, {'score': 66, 'version': 2L, 'sequence': 'ACTCAAAGAGGGGCAATGGC'})
({'score': 95, 'version': 0L, 'sequence': 'CAATATGCCATAATAAGTGC'}, {'score': 100, 'version': 2L, 'sequence': 'CAATATGCCATAATAAGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGATCCCCAACCAGCGAC'}, {'score': 99, 'version': 2L, 'sequence': 'AGTGATCCCCAACCAGCGAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTCAGCTCATGTTGCTCAC'}, {'score': 65, 'version': 2L, 'sequence': 'GGTCAGCTCATGTTGCTCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCCCCATCGATGTTGCTCC'}, {'score': 51, 'version': 2L, 'sequence': 'CTCCCCATCGATGTTGCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAGAAGTGGCAAAGTTGGC'}, {'score': 66, 'version': 2L, 'sequence': 'TCAGAAGTGGCAAAGTTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTACATGGCTTAATATAAC'}, {'score': 49, 'version': 2L, 'sequence': 'CCTACATGGCTTAATATAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGGGTTGATCAAGTTTTTC'}, {'score': 66, 'version': 2L, 'sequence': 'CTGGGTTGATCAAGTTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTAGACTTTAAGCTCTAC'}, {'score': 11, 'version': 2L, 'sequence': 'TTTTAGACTTTAAGCTCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAAGTGTTTCCAATTTCTC'}, {'score': 66, 'version': 2L, 'sequence': 'ATAAGTGTTTCCAATTTCTC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTACAGACTTATTATGATC'}, {'score': 66, 'version': 2L, 'sequence': 'GTTACAGACTTATTATGATC'})
({'score': 100, 'version': 0L, 'sequence': 'TAATGATAGTAAAGGCAACC'}, {'score': 66, 'version': 2L, 'sequence': 'TAATGATAGTAAAGGCAACC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGTAAAATGGTGAGGTGTC'}, {'score': 99, 'version': 2L, 'sequence': 'TGGTAAAATGGTGAGGTGTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTATTAGACTTAATTCTAC'}, {'score': 4, 'version': 2L, 'sequence': 'TGTATTAGACTTAATTCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGCTTATGCAGACTGCAC'}, {'score': 66, 'version': 2L, 'sequence': 'TTTGCTTATGCAGACTGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACATACATATGGTGCTTATC'}, {'score': 66, 'version': 2L, 'sequence': 'ACATACATATGGTGCTTATC'})
({'score': 51, 'version': 0L, 'sequence': 'ACCAATAATCTGCTGGGATC'}, {'score': 52, 'version': 2L, 'sequence': 'ACCAATAATCTGCTGGGATC'})
({'score': 100, 'version': 0L, 'sequence': 'TACTGTAGCTGCAGTTTTCC'}, {'score': 66, 'version': 2L, 'sequence': 'TACTGTAGCTGCAGTTTTCC'})
({'score': 99, 'version': 0L, 'sequence': 'TCTATGTATTTCTGTAGTCC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTATGTATTTCTGTAGTCC'})
({'score': 62, 'version': 0L, 'sequence': 'TTCCTTTCTTTCACAGGGTC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCCTTTCTTTCACAGGGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCCTCTCTATCATCTGTCC'}, {'score': 50, 'version': 2L, 'sequence': 'CTCCTCTCTATCATCTGTCC'})
({'score': 49, 'version': 0L, 'sequence': 'GAACATCTCAAGCAGAGAAC'}, {'score': 50, 'version': 2L, 'sequence': 'GAACATCTCAAGCAGAGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGTCTTTTATACAGGTGAC'}, {'score': 2, 'version': 2L, 'sequence': 'GGGTCTTTTATACAGGTGAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATATATATATTTCATTAC'}, {'score': 43, 'version': 2L, 'sequence': 'ATATATATATATTTCATTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTCTCTTAATGTCATAGC'}, {'score': 40, 'version': 2L, 'sequence': 'TTTTCTCTTAATGTCATAGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCAACAAACAGCCCCCAGCC'}, {'score': 94, 'version': 2L, 'sequence': 'GCAACAAACAGCCCCCAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TATACAATCATTTAGTAAGC'}, {'score': 99, 'version': 2L, 'sequence': 'TATACAATCATTTAGTAAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CAATGCTCTGTGCAATCTGC'}, {'score': 65, 'version': 2L, 'sequence': 'CAATGCTCTGTGCAATCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTATATATTGGTGGTGGCC'}, {'score': 66, 'version': 2L, 'sequence': 'TTTATATATTGGTGGTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCCAGCACAGTGGGAATGC'}, {'score': 66, 'version': 2L, 'sequence': 'CCCCAGCACAGTGGGAATGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTTTCTCCTGGTGTCCCGC'}, {'score': 3, 'version': 2L, 'sequence': 'GTTTTCTCCTGGTGTCCCGC'})
({'score': 100, 'version': 0L, 'sequence': 'ACCTTGCCACTGGCACTTGC'}, {'score': 96, 'version': 2L, 'sequence': 'ACCTTGCCACTGGCACTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GACTGGCAATTTGTGGATCC'}, {'score': 6, 'version': 2L, 'sequence': 'GACTGGCAATTTGTGGATCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGATGAAGTAACCAACCAC'}, {'score': 47, 'version': 2L, 'sequence': 'GGGATGAAGTAACCAACCAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGCATTCTAATTCCTTTCC'}, {'score': 64, 'version': 2L, 'sequence': 'AAGCATTCTAATTCCTTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAAGGGATTTCATTTGATC'}, {'score': 93, 'version': 2L, 'sequence': 'AAAAGGGATTTCATTTGATC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAATGGAGTATAAGTGCTC'}, {'score': 62, 'version': 2L, 'sequence': 'AAAATGGAGTATAAGTGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'GATCCTTTTCAGGCCTTAGC'}, {'score': 64, 'version': 2L, 'sequence': 'GATCCTTTTCAGGCCTTAGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGGAGCAACACTGGTCAC'}, {'score': 66, 'version': 2L, 'sequence': 'AAAGGAGCAACACTGGTCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGCCTCCTCAAGCTTTTC'}, {'score': 66, 'version': 2L, 'sequence': 'TTTGCCTCCTCAAGCTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTCTGGATGTCCCGATAC'}, {'score': 66, 'version': 2L, 'sequence': 'TTCTCTGGATGTCCCGATAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATACCATTTATTATGCCCCC'}, {'score': 40, 'version': 2L, 'sequence': 'ATACCATTTATTATGCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAGGAGCTTATATTGTTCC'}, {'score': 66, 'version': 2L, 'sequence': 'TAAGGAGCTTATATTGTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTTCCAGAAGATAATAAC'}, {'score': 66, 'version': 2L, 'sequence': 'TGCTTCCAGAAGATAATAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CATGGAAACGTGTGAGCAAC'}, {'score': 52, 'version': 2L, 'sequence': 'CATGGAAACGTGTGAGCAAC'})
({'score': 64, 'version': 0L, 'sequence': 'CATTGCCCCTCTTTGAGTCC'}, {'score': 66, 'version': 2L, 'sequence': 'CATTGCCCCTCTTTGAGTCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACTCAAAGAGGGGCAATGGC'}, {'score': 66, 'version': 2L, 'sequence': 'ACTCAAAGAGGGGCAATGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAGAAGTGGCAAAGTTGGC'}, {'score': 66, 'version': 2L, 'sequence': 'TCAGAAGTGGCAAAGTTGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTATATGGCTTAATATAAC'}, {'score': 49, 'version': 2L, 'sequence': 'CCTATATGGCTTAATATAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GATCACTGATAAACAGTATC'}, {'score': 17, 'version': 2L, 'sequence': 'GATCACTGATAAACAGTATC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTAGACTTTAGGCTCTAC'}, {'score': 38, 'version': 2L, 'sequence': 'TTTTAGACTTTAGGCTCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAAGTGTTTCCAATTTCTC'}, {'score': 66, 'version': 2L, 'sequence': 'ATAAGTGTTTCCAATTTCTC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTACAGACTTATTATGATC'}, {'score': 66, 'version': 2L, 'sequence': 'GTTACAGACTTATTATGATC'})
({'score': 100, 'version': 0L, 'sequence': 'TAATGATAGTAAAGGCAACC'}, {'score': 66, 'version': 2L, 'sequence': 'TAATGATAGTAAAGGCAACC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGTAAAATGGTGAGGTGCC'}, {'score': 99, 'version': 2L, 'sequence': 'TGGTAAAATGGTGAGGTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGCTTATGCAGACTGCAC'}, {'score': 66, 'version': 2L, 'sequence': 'TTTGCTTATGCAGACTGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACATACATATGGTGCTTATC'}, {'score': 66, 'version': 2L, 'sequence': 'ACATACATATGGTGCTTATC'})
({'score': 100, 'version': 0L, 'sequence': 'TACTGTAGCTGCAGTTTTCC'}, {'score': 66, 'version': 2L, 'sequence': 'TACTGTAGCTGCAGTTTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCCTCTCTATCATCTGTCC'}, {'score': 50, 'version': 2L, 'sequence': 'CTCCTCTCTATCATCTGTCC'})
({'score': 49, 'version': 0L, 'sequence': 'GAACATCTCAAGCAGAGAAC'}, {'score': 50, 'version': 2L, 'sequence': 'GAACATCTCAAGCAGAGAAC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 49, 'version': 0L, 'sequence': 'GAACATCTCAAGCAGAGAAC'}, {'score': 50, 'version': 2L, 'sequence': 'GAACATCTCAAGCAGAGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCCTCTCTATCATCTGTCC'}, {'score': 50, 'version': 2L, 'sequence': 'CTCCTCTCTATCATCTGTCC'})
({'score': 0, 'version': 0L, 'sequence': 'AAGGTTGGGGACCCCTGCTC'}, {'score': 8, 'version': 2L, 'sequence': 'AAGGTTGGGGACCCCTGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGACTACTCTCACCACTTC'}, {'score': 2, 'version': 2L, 'sequence': 'CTGACTACTCTCACCACTTC'})
({'score': 0, 'version': 0L, 'sequence': 'ATTCGCTGCAAATCCATGCC'}, {'score': 41, 'version': 2L, 'sequence': 'ATTCGCTGCAAATCCATGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCACTTGTCTGGTTTTGACC'}, {'score': 0, 'version': 2L, 'sequence': 'CCACTTGTCTGGTTTTGACC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTTCGGGGCCCCGAGCACC'}, {'score': 0, 'version': 2L, 'sequence': 'GGTTCGGGGCCCCGAGCACC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTTCGGGGCCCCGAGCACC'}, {'score': 0, 'version': 2L, 'sequence': 'GGTTCGGGGCCCCGAGCACC'})
({'score': 1, 'version': 0L, 'sequence': 'CTAATATCTACAGTTAGTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATATCTACAGTTAGTAC'})
({'score': 0, 'version': 0L, 'sequence': 'CAAATTGAGGAATATTGGCC'}, {'score': 87, 'version': 2L, 'sequence': 'CAAATTGAGGAATATTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCAATAGTTATTTGGCTGC'}, {'score': 0, 'version': 2L, 'sequence': 'AGCAATAGTTATTTGGCTGC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCAAGCGCATCCCGCTGTGC'}, {'score': 3, 'version': 2L, 'sequence': 'GCAAGCGCATCCCGCTGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCCTTGAGTTGGGCAGCTC'}, {'score': 95, 'version': 2L, 'sequence': 'TGCCTTGAGTTGGGCAGCTC'})
({'score': 1, 'version': 0L, 'sequence': 'TATGGGAGACTTTCTTGGGC'}, {'score': 35, 'version': 2L, 'sequence': 'TATGGGAGACTTTCTTGGGC'})
({'score': 0, 'version': 0L, 'sequence': 'ATGGGAGACTTTCCTCGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'ATGGGAGACTTTCCTCGGGC'})
({'score': 0, 'version': 0L, 'sequence': 'TATTGTGACTTTCCTTGGGC'}, {'score': 29, 'version': 2L, 'sequence': 'TATTGTGACTTTCCTTGGGC'})
({'score': 2, 'version': 0L, 'sequence': 'TGGCCCACCATGTTTTTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGCCCACCATGTTTTTTCC'})
({'score': 2, 'version': 0L, 'sequence': 'TGGCCCACCATGTTTTTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGCCCACCATGTTTTTTCC'})
({'score': 15, 'version': 0L, 'sequence': 'GGACTGGGCCGCCCAGACAC'}, {'score': 100, 'version': 2L, 'sequence': 'GGACTGGGCCGCCCAGACAC'})
({'score': 96, 'version': 0L, 'sequence': 'AAGATATATGTCTGCTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGATATATGTCTGCTGCAC'})
({'score': 2, 'version': 0L, 'sequence': 'AAAGGTGGCCATACATGGGC'}, {'score': 8, 'version': 2L, 'sequence': 'AAAGGTGGCCATACATGGGC'})
({'score': 52, 'version': 0L, 'sequence': 'CTAATATGGGCAGCTAGAAC'}, {'score': 85, 'version': 2L, 'sequence': 'CTAATATGGGCAGCTAGAAC'})
({'score': 64, 'version': 0L, 'sequence': 'CCTTTTCATGGGCCTCTTAC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTTTTCATGGGCCTCTTAC'})
({'score': 63, 'version': 0L, 'sequence': 'CCCGCCTCCAAATCATCAGC'}, {'score': 100, 'version': 2L, 'sequence': 'CCCGCCTCCAAATCATCAGC'})
({'score': 17, 'version': 0L, 'sequence': 'AGGCTAGGCCTGTGAAATAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGGCTAGGCCTGTGAAATAC'})
({'score': 37, 'version': 0L, 'sequence': 'TTGTAGGGTTGCCACCCAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTGTAGGGTTGCCACCCAAC'})
({'score': 97, 'version': 0L, 'sequence': 'TTTAGAACAGAGACCTACAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTAGAACAGAGACCTACAC'})
({'score': 59, 'version': 0L, 'sequence': 'CTGTGAGGCCAAACACTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGTGAGGCCAAACACTGCC'})
({'score': 59, 'version': 0L, 'sequence': 'CTGTGAGGCCAAACACTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGTGAGGCCAAACACTGCC'})
({'score': 72, 'version': 0L, 'sequence': 'GGGCGCTGAAATCATAAATC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGCGCTGAAATCATAAATC'})
({'score': 87, 'version': 0L, 'sequence': 'TTTTCCAATCCTTGGCGGGC'}, {'score': 97, 'version': 2L, 'sequence': 'TTTTCCAATCCTTGGCGGGC'})
({'score': 0, 'version': 0L, 'sequence': 'GATTAAAAAGACCAACGGGC'}, {'score': 45, 'version': 2L, 'sequence': 'GATTAAAAAGACCAACGGGC'})
({'score': 49, 'version': 0L, 'sequence': 'TATAGCCCGTGGGCCACGTC'}, {'score': 100, 'version': 2L, 'sequence': 'TATAGCCCGTGGGCCACGTC'})
({'score': 95, 'version': 0L, 'sequence': 'CCTCTGCCCTCTGTCTGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTCTGCCCTCTGTCTGGGC'})
({'score': 0, 'version': 0L, 'sequence': 'ACCTTCCCCTCTGTCTGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCTTCCCCTCTGTCTGGGC'})
({'score': 2, 'version': 0L, 'sequence': 'TTCCCACTGGACCTGAACAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCCCACTGGACCTGAACAC'})
({'score': 6, 'version': 0L, 'sequence': 'CCTGTTACTGTCTCCACATC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTGTTACTGTCTCCACATC'})
({'score': 7, 'version': 0L, 'sequence': 'AGTGCCGTTGATGAGAAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTGCCGTTGATGAGAAAGC'})
({'score': 7, 'version': 0L, 'sequence': 'GCGCCAGCGTAGATATGCGC'}, {'score': 100, 'version': 2L, 'sequence': 'GCGCCAGCGTAGATATGCGC'})
({'score': 19, 'version': 0L, 'sequence': 'CTCCCCCAACACGCGACCTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTCCCCCAACACGCGACCTC'})
({'score': 19, 'version': 0L, 'sequence': 'GACCAGCACCTTCACGCGTC'}, {'score': 100, 'version': 2L, 'sequence': 'GACCAGCACCTTCACGCGTC'})
({'score': 22, 'version': 0L, 'sequence': 'TCGACACCCTCAACGGACAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCGACACCCTCAACGGACAC'})
({'score': 14, 'version': 0L, 'sequence': 'AATTTCTGATCCTCCTGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'AATTTCTGATCCTCCTGCTC'})
({'score': 11, 'version': 0L, 'sequence': 'CCAAACGGAATAGCTGCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCAAACGGAATAGCTGCTCC'})
({'score': 11, 'version': 0L, 'sequence': 'CCAAACGGAATAGCTGCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCAAACGGAATAGCTGCTCC'})
({'score': 18, 'version': 0L, 'sequence': 'CTGCCCCATTGAGTTATTAC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGCCCCATTGAGTTATTAC'})
({'score': 39, 'version': 0L, 'sequence': 'CAATGGGGCAGTCGTAGATC'}, {'score': 100, 'version': 2L, 'sequence': 'CAATGGGGCAGTCGTAGATC'})
({'score': 19, 'version': 0L, 'sequence': 'CAGATGTTCTTCCTCCCCAC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGATGTTCTTCCTCCCCAC'})
({'score': 16, 'version': 0L, 'sequence': 'AGGACCCACACAACCCCAAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGGACCCACACAACCCCAAC'})
({'score': 19, 'version': 0L, 'sequence': 'AGGCCTTAAGACATCAGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGGCCTTAAGACATCAGTTC'})
({'score': 18, 'version': 0L, 'sequence': 'TGCCCAAGTCTTTGGTTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TGCCCAAGTCTTTGGTTCTC'})
({'score': 16, 'version': 0L, 'sequence': 'GTGACAATCTCCTTGCTGTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTGACAATCTCCTTGCTGTC'})
({'score': 16, 'version': 0L, 'sequence': 'GCTACAGGCGCGTCAGGAAC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTACAGGCGCGTCAGGAAC'})
({'score': 20, 'version': 0L, 'sequence': 'CCTAAATGCAGTTGTCAACC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTAAATGCAGTTGTCAACC'})
({'score': 20, 'version': 0L, 'sequence': 'CCTAAATGCAGTTGTCAACC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTAAATGCAGTTGTCAACC'})
({'score': 2, 'version': 0L, 'sequence': 'TTCCCACTGGACCTGAACAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCCCACTGGACCTGAACAC'})
({'score': 6, 'version': 0L, 'sequence': 'CCTGTTACTGTCTCCACATC'}, {'score': 100, 'version': 2L, 'sequence': 'CCTGTTACTGTCTCCACATC'})
({'score': 7, 'version': 0L, 'sequence': 'AGTGCCGTTGATGAGAAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTGCCGTTGATGAGAAAGC'})
({'score': 6, 'version': 0L, 'sequence': 'GCCCATGTGCGGCATCGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCCATGTGCGGCATCGTTC'})
({'score': 7, 'version': 0L, 'sequence': 'GCGCCAGCGTAGATATGCGC'}, {'score': 100, 'version': 2L, 'sequence': 'GCGCCAGCGTAGATATGCGC'})
({'score': 0, 'version': 0L, 'sequence': 'TATGTGGGCTGGCAGCCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TATGTGGGCTGGCAGCCTAC'})
({'score': 98, 'version': 0L, 'sequence': 'GTTCTGCCAAAAAGAACCTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTCTGCCAAAAAGAACCTC'})
({'score': 0, 'version': 0L, 'sequence': 'AAATTGGGCAGATATCGATC'}, {'score': 62, 'version': 2L, 'sequence': 'AAATTGGGCAGATATCGATC'})
({'score': 50, 'version': 0L, 'sequence': 'GACGAAATTTTTTAACCTGC'}, {'score': 100, 'version': 2L, 'sequence': 'GACGAAATTTTTTAACCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TATTTGGAGTTGGTCACTTC'}, {'score': 93, 'version': 2L, 'sequence': 'TATTTGGAGTTGGTCACTTC'})
({'score': 35, 'version': 0L, 'sequence': 'GGGAAATCGCAGCGACTTCC'}, {'score': 19, 'version': 2L, 'sequence': 'GGGAAATCGCAGCGACTTCC'})
({'score': 35, 'version': 0L, 'sequence': 'GGGAAATCGCAGCGACTTCC'}, {'score': 19, 'version': 2L, 'sequence': 'GGGAAATCGCAGCGACTTCC'})
({'score': 17, 'version': 0L, 'sequence': 'TGAAAGTAGAACCCACTGAC'}, {'score': 41, 'version': 2L, 'sequence': 'TGAAAGTAGAACCCACTGAC'})
({'score': 70, 'version': 0L, 'sequence': 'GCTGTTTGCCAGCACAGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTGTTTGCCAGCACAGAGC'})
({'score': 45, 'version': 0L, 'sequence': 'TCAACAGGGGCGGGCCAAGC'}, {'score': 3, 'version': 2L, 'sequence': 'TCAACAGGGGCGGGCCAAGC'})
({'score': 90, 'version': 0L, 'sequence': 'CATAAAGCTATGCCATTATC'}, {'score': 100, 'version': 2L, 'sequence': 'CATAAAGCTATGCCATTATC'})
({'score': 4, 'version': 0L, 'sequence': 'ATTTCTGGGTAATGTGTTTC'}, {'score': 7, 'version': 2L, 'sequence': 'ATTTCTGGGTAATGTGTTTC'})
({'score': 68, 'version': 0L, 'sequence': 'TAAAATGGGCAGATCTCAAC'}, {'score': 54, 'version': 2L, 'sequence': 'TAAAATGGGCAGATCTCAAC'})
({'score': 0, 'version': 0L, 'sequence': 'ACCGCTGTTCCGCGGCACAC'}, {'score': 99, 'version': 2L, 'sequence': 'ACCGCTGTTCCGCGGCACAC'})
({'score': 4, 'version': 0L, 'sequence': 'CTTTTCCCTTTAATAAAATC'}, {'score': 100, 'version': 2L, 'sequence': 'CTTTTCCCTTTAATAAAATC'})
({'score': 64, 'version': 0L, 'sequence': 'TAGCCCACCCTTTCCCTACC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGCCCACCCTTTCCCTACC'})
({'score': 64, 'version': 0L, 'sequence': 'TAGCCCACCCTTTCCCTACC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGCCCACCCTTTCCCTACC'})
({'score': 85, 'version': 0L, 'sequence': 'TATAATGACTTACATGGTTC'}, {'score': 95, 'version': 2L, 'sequence': 'TATAATGACTTACATGGTTC'})
({'score': 63, 'version': 0L, 'sequence': 'TAGCCCACCCCTTCCCTACC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGCCCACCCCTTCCCTACC'})
({'score': 63, 'version': 0L, 'sequence': 'TAGCCCACCCCTTCCCTACC'}, {'score': 100, 'version': 2L, 'sequence': 'TAGCCCACCCCTTCCCTACC'})
({'score': 35, 'version': 0L, 'sequence': 'TATAATGACTTACATAGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TATAATGACTTACATAGTTC'})
({'score': 81, 'version': 0L, 'sequence': 'TCCTGAGGGCCAGGGCCCAC'}, {'score': 63, 'version': 2L, 'sequence': 'TCCTGAGGGCCAGGGCCCAC'})
({'score': 28, 'version': 0L, 'sequence': 'GATCATGTTGGTGTGGGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GATCATGTTGGTGTGGGGGC'})
({'score': 99, 'version': 0L, 'sequence': 'GCAGTTTTTGCAGAAGTTAC'}, {'score': 100, 'version': 2L, 'sequence': 'GCAGTTTTTGCAGAAGTTAC'})
({'score': 5, 'version': 0L, 'sequence': 'GGCTGAGATGAAGGACGGAC'}, {'score': 95, 'version': 2L, 'sequence': 'GGCTGAGATGAAGGACGGAC'})
({'score': 3, 'version': 0L, 'sequence': 'CTACCTGTACCAGTTCCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTACCTGTACCAGTTCCCCC'})
({'score': 3, 'version': 0L, 'sequence': 'CTACCTGTACCAGTTCCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTACCTGTACCAGTTCCCCC'})
({'score': 20, 'version': 0L, 'sequence': 'GTACAGGTAGGACTCAGCGC'}, {'score': 100, 'version': 2L, 'sequence': 'GTACAGGTAGGACTCAGCGC'})
({'score': 18, 'version': 0L, 'sequence': 'TGTTCACAACTCCTCCCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTTCACAACTCCTCCCCCC'})
({'score': 18, 'version': 0L, 'sequence': 'TGTTCACAACTCCTCCCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTTCACAACTCCTCCCCCC'})
({'score': 18, 'version': 0L, 'sequence': 'CCCTCAGACTGTTAGAGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'CCCTCAGACTGTTAGAGGGC'})
({'score': 3, 'version': 0L, 'sequence': 'AAACTTCTGACCCAAGGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'AAACTTCTGACCCAAGGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGGCTTGGGTCAACAGGGC'}, {'score': 35, 'version': 2L, 'sequence': 'CAGGCTTGGGTCAACAGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTAGGGGAGAGGGAGTAGCC'}, {'score': 79, 'version': 2L, 'sequence': 'TTAGGGGAGAGGGAGTAGCC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 97, 'version': 0L, 'sequence': 'ATCCAAAGGAACAAGGCTGC'}, {'score': 92, 'version': 2L, 'sequence': 'ATCCAAAGGAACAAGGCTGC'})
({'score': 6, 'version': 0L, 'sequence': 'AGTTCCCAGTATAACTCAGC'}, {'score': 5, 'version': 2L, 'sequence': 'AGTTCCCAGTATAACTCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATGTGGGGGCCTATGGGTGC'}, {'score': 99, 'version': 2L, 'sequence': 'ATGTGGGGGCCTATGGGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGAGCTGGCATGGTTTTGC'}, {'score': 97, 'version': 2L, 'sequence': 'TGGAGCTGGCATGGTTTTGC'})
({'score': 99, 'version': 0L, 'sequence': 'AGTGTGTAAGCCTGCATGAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTGTGTAAGCCTGCATGAC'})
({'score': 16, 'version': 0L, 'sequence': 'AAATAAGATTGTTTCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATAAGATTGTTTCCTTCC'})
({'score': 16, 'version': 0L, 'sequence': 'AAATAAGATTGTTTCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATAAGATTGTTTCCTTCC'})
({'score': 11, 'version': 0L, 'sequence': 'TCTTATTTCCCCCTATACTC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTTATTTCCCCCTATACTC'})
({'score': 16, 'version': 0L, 'sequence': 'AAATAAGATTGTTTCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATAAGATTGTTTCCTTCC'})
({'score': 16, 'version': 0L, 'sequence': 'AAATAAGATTGTTTCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATAAGATTGTTTCCTTCC'})
({'score': 11, 'version': 0L, 'sequence': 'TCTTATTTCCCCCTATACTC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTTATTTCCCCCTATACTC'})
({'score': 16, 'version': 0L, 'sequence': 'AAATAAGATTGTTTCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATAAGATTGTTTCCTTCC'})
({'score': 16, 'version': 0L, 'sequence': 'AAATAAGATTGTTTCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATAAGATTGTTTCCTTCC'})
({'score': 11, 'version': 0L, 'sequence': 'TCTTATTTCCCCCTATACTC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTTATTTCCCCCTATACTC'})
({'score': 16, 'version': 0L, 'sequence': 'AAATAAGATTGTTTCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATAAGATTGTTTCCTTCC'})
({'score': 16, 'version': 0L, 'sequence': 'AAATAAGATTGTTTCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAATAAGATTGTTTCCTTCC'})
({'score': 11, 'version': 0L, 'sequence': 'TCTTATTTCCCCCTATACTC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTTATTTCCCCCTATACTC'})
({'score': 62, 'version': 0L, 'sequence': 'CTTATTTTCCCCCTATTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTTATTTTCCCCCTATTCTC'})
({'score': 18, 'version': 0L, 'sequence': 'ATCGGCGATCGTCCGATGAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCGGCGATCGTCCGATGAC'})
({'score': 22, 'version': 0L, 'sequence': 'ACGCAGCACTTCCGCATCTC'}, {'score': 100, 'version': 2L, 'sequence': 'ACGCAGCACTTCCGCATCTC'})
({'score': 11, 'version': 0L, 'sequence': 'ACCTCTGTGGTACTGAGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCTCTGTGGTACTGAGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCACTTGGCAGGCAATTTC'}, {'score': 49, 'version': 2L, 'sequence': 'AGCACTTGGCAGGCAATTTC'})
({'score': 0, 'version': 0L, 'sequence': 'GTGATCCCCAACCAGTGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTGATCCCCAACCAGTGGCC'})
({'score': 0, 'version': 0L, 'sequence': 'GTGATCCCCAACCAGTGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'GTGATCCCCAACCAGTGGCC'})
({'score': 28, 'version': 0L, 'sequence': 'GGTGAGCAACATGTTGCCCC'}, {'score': 24, 'version': 2L, 'sequence': 'GGTGAGCAACATGTTGCCCC'})
({'score': 96, 'version': 0L, 'sequence': 'TAAATAAATGTATCAATTGC'}, {'score': 100, 'version': 2L, 'sequence': 'TAAATAAATGTATCAATTGC'})
({'score': 61, 'version': 0L, 'sequence': 'TGTCGTCCCTGGTAACCTGC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTCGTCCCTGGTAACCTGC'})
({'score': 99, 'version': 0L, 'sequence': 'GGGGGGGGGGGAAAGGCTTC'}, {'score': 47, 'version': 2L, 'sequence': 'GGGGGGGGGGGAAAGGCTTC'})
({'score': 99, 'version': 0L, 'sequence': 'TTTTAACCAGTAATAATCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTTAACCAGTAATAATCTC'})
({'score': 0, 'version': 0L, 'sequence': 'ATTCGCTGCAAATCCATGCC'}, {'score': 41, 'version': 2L, 'sequence': 'ATTCGCTGCAAATCCATGCC'})
({'score': 3, 'version': 0L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'})
({'score': 3, 'version': 0L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'})
({'score': 12, 'version': 0L, 'sequence': 'GGGCAGATGAGGGGCAGGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGCAGATGAGGGGCAGGGC'})
({'score': 3, 'version': 0L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'})
({'score': 3, 'version': 0L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'})
({'score': 9, 'version': 0L, 'sequence': 'GGGCAGATGAGGGGCATGGC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGCAGATGAGGGGCATGGC'})
({'score': 98, 'version': 0L, 'sequence': 'TATTTCTCTTCAGACCTTAC'}, {'score': 100, 'version': 2L, 'sequence': 'TATTTCTCTTCAGACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'GATACTGAGTACACACACCC'}, {'score': 95, 'version': 2L, 'sequence': 'GATACTGAGTACACACACCC'})
({'score': 100, 'version': 0L, 'sequence': 'GATACTGAGTACACACACCC'}, {'score': 95, 'version': 2L, 'sequence': 'GATACTGAGTACACACACCC'})
({'score': 91, 'version': 0L, 'sequence': 'CTGCAGTTAAACTTAATGAC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGCAGTTAAACTTAATGAC'})
({'score': 49, 'version': 0L, 'sequence': 'CCGCAATAAATCTCTGCTGC'}, {'score': 55, 'version': 2L, 'sequence': 'CCGCAATAAATCTCTGCTGC'})
({'score': 61, 'version': 0L, 'sequence': 'TGGGCGAGATTAGTCGCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGGCGAGATTAGTCGCCCC'})
({'score': 61, 'version': 0L, 'sequence': 'TGGGCGAGATTAGTCGCCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGGCGAGATTAGTCGCCCC'})
({'score': 98, 'version': 0L, 'sequence': 'TTAATTTGATCATTTGGCCC'}, {'score': 0, 'version': 2L, 'sequence': 'TTAATTTGATCATTTGGCCC'})
({'score': 99, 'version': 0L, 'sequence': 'CTGCTTTATGGCTGACATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CTGCTTTATGGCTGACATTC'})
({'score': 97, 'version': 0L, 'sequence': 'CACAAGTATTAACAGGCTGC'}, {'score': 100, 'version': 2L, 'sequence': 'CACAAGTATTAACAGGCTGC'})
({'score': 64, 'version': 0L, 'sequence': 'AAGGCTGGGGACCCCTGCCC'}, {'score': 1, 'version': 2L, 'sequence': 'AAGGCTGGGGACCCCTGCCC'})
({'score': 95, 'version': 0L, 'sequence': 'AGTAATCAGTGGGTGACAGC'}, {'score': 94, 'version': 2L, 'sequence': 'AGTAATCAGTGGGTGACAGC'})
({'score': 25, 'version': 0L, 'sequence': 'GGAGAAACCCCTGCCAGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'GGAGAAACCCCTGCCAGCAC'})
({'score': 13, 'version': 0L, 'sequence': 'GGAGGTGTAAGTAGCGATCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGAGGTGTAAGTAGCGATCC'})
({'score': 13, 'version': 0L, 'sequence': 'GGAGGTGTAAGTAGCGATCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGAGGTGTAAGTAGCGATCC'})
({'score': 62, 'version': 0L, 'sequence': 'TTACCAGCAACTGCAGGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTACCAGCAACTGCAGGGCC'})
({'score': 62, 'version': 0L, 'sequence': 'TTACCAGCAACTGCAGGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTACCAGCAACTGCAGGGCC'})
({'score': 99, 'version': 0L, 'sequence': 'AAACCTCTGGCCAAGCCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAACCTCTGGCCAAGCCTTC'})
({'score': 69, 'version': 0L, 'sequence': 'ATACACAAGGAACAGGGAGC'}, {'score': 72, 'version': 2L, 'sequence': 'ATACACAAGGAACAGGGAGC'})
({'score': 0, 'version': 0L, 'sequence': 'TCTCGGGACCAAGGGTATTC'}, {'score': 49, 'version': 2L, 'sequence': 'TCTCGGGACCAAGGGTATTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCACATGCTCAGTGTGCTC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCACATGCTCAGTGTGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCATGGTTGCCATGGGTTAC'}, {'score': 14, 'version': 2L, 'sequence': 'GCATGGTTGCCATGGGTTAC'})
({'score': 30, 'version': 0L, 'sequence': 'AGCAATAACAACAGCACCCC'}, {'score': 32, 'version': 2L, 'sequence': 'AGCAATAACAACAGCACCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TATCTGAATAACATGAAAGC'}, {'score': 80, 'version': 2L, 'sequence': 'TATCTGAATAACATGAAAGC'})
({'score': 100, 'version': 0L, 'sequence': 'GCATCTCCTTGGTGCTACCC'}, {'score': 98, 'version': 2L, 'sequence': 'GCATCTCCTTGGTGCTACCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTGACAGCTATATGGTTGC'}, {'score': 89, 'version': 2L, 'sequence': 'TGTGACAGCTATATGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTAAGGTAAATTGAACCC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCTAAGGTAAATTGAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'AACTTCCTGCCCACTGGGAC'}, {'score': 95, 'version': 2L, 'sequence': 'AACTTCCTGCCCACTGGGAC'})
({'score': 90, 'version': 0L, 'sequence': 'CAAATAATGTTTAGATTCTC'}, {'score': 96, 'version': 2L, 'sequence': 'CAAATAATGTTTAGATTCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CATACAGTTATTTTAATTTC'}, {'score': 46, 'version': 2L, 'sequence': 'CATACAGTTATTTTAATTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGCCCCCTAAGTTTTGTCC'}, {'score': 99, 'version': 2L, 'sequence': 'CAGCCCCCTAAGTTTTGTCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGGCCAATTTCCTATTTTC'}, {'score': 78, 'version': 2L, 'sequence': 'CTGGCCAATTTCCTATTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTACCAGCTATCTGGTTGC'}, {'score': 5, 'version': 2L, 'sequence': 'TCTACCAGCTATCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGTTCCAGTTTACCC'}, {'score': 99, 'version': 2L, 'sequence': 'TAGGTTCCAGTTTACCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGCAACCGGCACTCCCATC'}, {'score': 59, 'version': 2L, 'sequence': 'AGGCAACCGGCACTCCCATC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCAGGCGTTCTAATGATCC'}, {'score': 70, 'version': 2L, 'sequence': 'CCCAGGCGTTCTAATGATCC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAAATATAATATTTATATC'}, {'score': 84, 'version': 2L, 'sequence': 'AAAAATATAATATTTATATC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCTTTAAATTTATTTATAC'}, {'score': 1, 'version': 2L, 'sequence': 'TTCTTTAAATTTATTTATAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTATATATAGATATATATC'}, {'score': 6, 'version': 2L, 'sequence': 'TTTATATATAGATATATATC'})
({'score': 72, 'version': 0L, 'sequence': 'CCAAATACCCCAGGTAACCC'}, {'score': 100, 'version': 2L, 'sequence': 'CCAAATACCCCAGGTAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCGCCAGTAAATCGCCAGC'}, {'score': 89, 'version': 2L, 'sequence': 'CCCGCCAGTAAATCGCCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAATGTGTAATTCTGCCTC'}, {'score': 97, 'version': 2L, 'sequence': 'TAAATGTGTAATTCTGCCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CAATGTTTTTGCCCTGTCTC'}, {'score': 99, 'version': 2L, 'sequence': 'CAATGTTTTTGCCCTGTCTC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTAGCAACCAGACACAGGC'}, {'score': 96, 'version': 2L, 'sequence': 'GTTAGCAACCAGACACAGGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTTGCTAACTGTACTTTTC'}, {'score': 94, 'version': 2L, 'sequence': 'GGTTGCTAACTGTACTTTTC'})
({'score': 89, 'version': 0L, 'sequence': 'ATGAGCCACATAAGAAAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATGAGCCACATAAGAAAAAC'})
({'score': 95, 'version': 0L, 'sequence': 'CACTAAGGTCACAGAGCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CACTAAGGTCACAGAGCTTC'})
({'score': 50, 'version': 0L, 'sequence': 'ATTTCATTAAGGGGCAGGAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATTTCATTAAGGGGCAGGAC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTCAGCAGCAATCTGTTGC'}, {'score': 20, 'version': 2L, 'sequence': 'TTTCAGCAGCAATCTGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTCAGTGTTCAACCTGCTC'}, {'score': 97, 'version': 2L, 'sequence': 'TTTCAGTGTTCAACCTGCTC'})
({'score': 97, 'version': 0L, 'sequence': 'CGGTTTGTGTGCAGAGGACC'}, {'score': 100, 'version': 2L, 'sequence': 'CGGTTTGTGTGCAGAGGACC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGTTCTGCAGCCAGAGTTC'}, {'score': 78, 'version': 2L, 'sequence': 'CAGTTCTGCAGCCAGAGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAGATACAACACAAACAAC'}, {'score': 93, 'version': 2L, 'sequence': 'ATAGATACAACACAAACAAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGAACATTGCAGAACCTTAC'}, {'score': 62, 'version': 2L, 'sequence': 'AGAACATTGCAGAACCTTAC'})
({'score': 96, 'version': 0L, 'sequence': 'AAGGTTCTGCAATGTTCTTC'}, {'score': 49, 'version': 2L, 'sequence': 'AAGGTTCTGCAATGTTCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGATATTACAGAACCTTAC'}, {'score': 67, 'version': 2L, 'sequence': 'AGGATATTACAGAACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGGTTCTGTAATATCCTCC'}, {'score': 59, 'version': 2L, 'sequence': 'AAGGTTCTGTAATATCCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGAACATTGCAGAACCTTAC'}, {'score': 62, 'version': 2L, 'sequence': 'AGAACATTGCAGAACCTTAC'})
({'score': 96, 'version': 0L, 'sequence': 'AAGGTTCTGCAATGTTCTTC'}, {'score': 49, 'version': 2L, 'sequence': 'AAGGTTCTGCAATGTTCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGATATTACAAAACCTTAC'}, {'score': 67, 'version': 2L, 'sequence': 'AGGATATTACAAAACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGGTTTTGTAATATCCTCC'}, {'score': 59, 'version': 2L, 'sequence': 'AAGGTTTTGTAATATCCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGTTCTGCAATATCCTTC'}, {'score': 48, 'version': 2L, 'sequence': 'TAGGTTCTGCAATATCCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGACATTGCAGAACCTTAC'}, {'score': 83, 'version': 2L, 'sequence': 'AAGACATTGCAGAACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACGGTTCTGCAGTGTGCTTC'}, {'score': 97, 'version': 2L, 'sequence': 'ACGGTTCTGCAGTGTGCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTGCAGAACCTACCTTAC'}, {'score': 48, 'version': 2L, 'sequence': 'TTTTGCAGAACCTACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGTTCTGCAAAATCCTCC'}, {'score': 50, 'version': 2L, 'sequence': 'TAGGTTCTGCAAAATCCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGACATTGCAGAACCTTAC'}, {'score': 36, 'version': 2L, 'sequence': 'AGGACATTGCAGAACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGGTTCTGCAATGTCCTTC'}, {'score': 42, 'version': 2L, 'sequence': 'AAGGTTCTGCAATGTCCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCACACTACAGAACATTAC'}, {'score': 50, 'version': 2L, 'sequence': 'AGCACACTACAGAACATTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AATGTTCTGTAGTGTGCTTC'}, {'score': 50, 'version': 2L, 'sequence': 'AATGTTCTGTAGTGTGCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTGCAGAACCTACCTTAC'}, {'score': 48, 'version': 2L, 'sequence': 'TTTTGCAGAACCTACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGTTCTGCAAAATCCTCC'}, {'score': 50, 'version': 2L, 'sequence': 'TAGGTTCTGCAAAATCCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGACATTGCAGAACCTTAC'}, {'score': 36, 'version': 2L, 'sequence': 'AGGACATTGCAGAACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGGTTCTGCAATGTCCTTC'}, {'score': 42, 'version': 2L, 'sequence': 'AAGGTTCTGCAATGTCCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCACACTACAGAACATTAC'}, {'score': 50, 'version': 2L, 'sequence': 'AGCACACTACAGAACATTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AATGTTCTGTAGTGTGCTTC'}, {'score': 50, 'version': 2L, 'sequence': 'AATGTTCTGTAGTGTGCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTTGCAGAACCTACCTTAC'}, {'score': 48, 'version': 2L, 'sequence': 'TTTTGCAGAACCTACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TAGGTTCTGCAAAATCCTCC'}, {'score': 50, 'version': 2L, 'sequence': 'TAGGTTCTGCAAAATCCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGACATTGCAGAACCTTAC'}, {'score': 36, 'version': 2L, 'sequence': 'AGGACATTGCAGAACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGGTTCTGCAATGTCCTTC'}, {'score': 42, 'version': 2L, 'sequence': 'AAGGTTCTGCAATGTCCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCACACTACAGAACATTAC'}, {'score': 50, 'version': 2L, 'sequence': 'AGCACACTACAGAACATTAC'})
({'score': 100, 'version': 0L, 'sequence': 'AATGTTCTGTAGTGTGCTTC'}, {'score': 50, 'version': 2L, 'sequence': 'AATGTTCTGTAGTGTGCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TATTGTAGAACCTACCTTAC'}, {'score': 62, 'version': 2L, 'sequence': 'TATTGTAGAACCTACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'TATTGCAGAACCTACCTTAC'}, {'score': 56, 'version': 2L, 'sequence': 'TATTGCAGAACCTACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGGTTCTGCAATATCCTTC'}, {'score': 48, 'version': 2L, 'sequence': 'CAGGTTCTGCAATATCCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGGTTCTGCAATATCCCCC'}, {'score': 99, 'version': 2L, 'sequence': 'CAGGTTCTGCAATATCCCCC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGACCTTGCAGAACCTTAC'}, {'score': 35, 'version': 2L, 'sequence': 'AGGACCTTGCAGAACCTTAC'})
({'score': 100, 'version': 0L, 'sequence': 'ACACTTGATAATAAAAGTCC'}, {'score': 99, 'version': 2L, 'sequence': 'ACACTTGATAATAAAAGTCC'})
({'score': 93, 'version': 0L, 'sequence': 'ACTGCTTTGGGGGGGCACAC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTGCTTTGGGGGGGCACAC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGGAGAAAAGCAAAGGAAC'}, {'score': 69, 'version': 2L, 'sequence': 'CAGGAGAAAAGCAAAGGAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCAAATAAATGTTCTCTGCC'}, {'score': 98, 'version': 2L, 'sequence': 'GCAAATAAATGTTCTCTGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTAAATATTTCGCCCATCAC'}, {'score': 14, 'version': 2L, 'sequence': 'TTAAATATTTCGCCCATCAC'})
({'score': 66, 'version': 0L, 'sequence': 'TCTTTGTATTTAAGCTGCTC'}, {'score': 100, 'version': 2L, 'sequence': 'TCTTTGTATTTAAGCTGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTGAGGTATCTTGGACCC'}, {'score': 5, 'version': 2L, 'sequence': 'TGCTGAGGTATCTTGGACCC'})
({'score': 100, 'version': 0L, 'sequence': 'GCCTCCTAAGTTTGCTCCAC'}, {'score': 99, 'version': 2L, 'sequence': 'GCCTCCTAAGTTTGCTCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGATCTTTTAATGTACGGC'}, {'score': 25, 'version': 2L, 'sequence': 'CAGATCTTTTAATGTACGGC'})
({'score': 100, 'version': 0L, 'sequence': 'GTCATTTGGCCAATTTAAGC'}, {'score': 96, 'version': 2L, 'sequence': 'GTCATTTGGCCAATTTAAGC'})
({'score': 84, 'version': 0L, 'sequence': 'CCTGGGTACCCCTGGAACTC'}, {'score': 0, 'version': 2L, 'sequence': 'CCTGGGTACCCCTGGAACTC'})
({'score': 86, 'version': 0L, 'sequence': 'CTGCACTGTCTGAGGGTTTC'}, {'score': 95, 'version': 2L, 'sequence': 'CTGCACTGTCTGAGGGTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGAGAAGAAATGAAAGATAC'}, {'score': 94, 'version': 2L, 'sequence': 'AGAGAAGAAATGAAAGATAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCCAGGGGTTGCAAGGTGC'}, {'score': 35, 'version': 2L, 'sequence': 'TGCCAGGGGTTGCAAGGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAAGCATTCTGTTTATCCC'}, {'score': 99, 'version': 2L, 'sequence': 'AAAAGCATTCTGTTTATCCC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGCCATTCAGGCCTATGTC'}, {'score': 94, 'version': 2L, 'sequence': 'CTGCCATTCAGGCCTATGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCACTGTTCCTTTAAAGAGC'}, {'score': 65, 'version': 2L, 'sequence': 'CCACTGTTCCTTTAAAGAGC'})
({'score': 100, 'version': 0L, 'sequence': 'AACGTTTAACTTCACCCTCC'}, {'score': 99, 'version': 2L, 'sequence': 'AACGTTTAACTTCACCCTCC'})
({'score': 100, 'version': 0L, 'sequence': 'ACATTGCTGATTTAGAAAAC'}, {'score': 99, 'version': 2L, 'sequence': 'ACATTGCTGATTTAGAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTGCCACAAGCAAGTTGTC'}, {'score': 99, 'version': 2L, 'sequence': 'GGTGCCACAAGCAAGTTGTC'})
({'score': 57, 'version': 0L, 'sequence': 'CAAATCAGGCCCAGATATGC'}, {'score': 100, 'version': 2L, 'sequence': 'CAAATCAGGCCCAGATATGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTAAAAAGTCAGACAAGTC'}, {'score': 92, 'version': 2L, 'sequence': 'CTTAAAAAGTCAGACAAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CGTGCATTGCTTCTAAGGCC'}, {'score': 98, 'version': 2L, 'sequence': 'CGTGCATTGCTTCTAAGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'CATTGTGATAAATCACCCAC'}, {'score': 97, 'version': 2L, 'sequence': 'CATTGTGATAAATCACCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CAACTGTGAGGCTGTACCTC'}, {'score': 89, 'version': 2L, 'sequence': 'CAACTGTGAGGCTGTACCTC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 3, 'version': 0L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'})
({'score': 3, 'version': 0L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCCCTGCCCCTCATCTGCC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 100, 'version': 0L, 'sequence': 'CCTCGCAAGCGCCTTTTTTC'}, {'score': 0, 'version': 2L, 'sequence': 'CCTCGCAAGCGCCTTTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGAACATGTAAGTCGC'}, {'score': 93, 'version': 2L, 'sequence': 'GCGAACATGTAAGTCGC'})
({'score': 0, 'version': 0L, 'sequence': 'AAGGTTGGGGACCCCTGCTC'}, {'score': 8, 'version': 2L, 'sequence': 'AAGGTTGGGGACCCCTGCTC'})
({'score': 67, 'version': 0L, 'sequence': 'GAGAAGACACACAGAGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'GAGAAGACACACAGAGCTAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGAACATGTAAGTCGC'}, {'score': 93, 'version': 2L, 'sequence': 'GCGAACATGTAAGTCGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTTGCTGCGTCACCAGTAC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTTGCTGCGTCACCAGTAC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 0, 'version': 0L, 'sequence': 'GGGTTGGGGAATGCCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGTTGGGGAATGCCCTTCC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'ATATCTGGCCTGAAATCGGC'}, {'score': 0, 'version': 2L, 'sequence': 'ATATCTGGCCTGAAATCGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TAACCTGCCCGATCGATATC'}, {'score': 0, 'version': 2L, 'sequence': 'TAACCTGCCCGATCGATATC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'}, {'score': 16, 'version': 2L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'}, {'score': 16, 'version': 2L, 'sequence': 'TTCAGCAGTTATCTGGTTGC'})
({'score': 0, 'version': 0L, 'sequence': 'CTGGGATTTAAAATAGGCCC'}, {'score': 3, 'version': 2L, 'sequence': 'CTGGGATTTAAAATAGGCCC'})
({'score': 1, 'version': 0L, 'sequence': 'CTAATATCTACAGTTAGTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATATCTACAGTTAGTAC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TAAACAGTAATTAAACCCAC'}, {'score': 0, 'version': 2L, 'sequence': 'TAAACAGTAATTAAACCCAC'})
({'score': 17, 'version': 0L, 'sequence': 'TATTGGAGCCAAAACAATCC'}, {'score': 99, 'version': 2L, 'sequence': 'TATTGGAGCCAAAACAATCC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTCCTCCAGCGATGCGTCC'}, {'score': 3, 'version': 2L, 'sequence': 'GTTCCTCCAGCGATGCGTCC'})
({'score': 0, 'version': 0L, 'sequence': 'CTGGGATTTAAAATAGGCCC'}, {'score': 3, 'version': 2L, 'sequence': 'CTGGGATTTAAAATAGGCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCCCTCTAGGTGCTCTCCC'}, {'score': 1, 'version': 2L, 'sequence': 'TCCCCTCTAGGTGCTCTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCCCTCTAGGTGCTCTCCC'}, {'score': 1, 'version': 2L, 'sequence': 'TCCCCTCTAGGTGCTCTCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCGTATGGCACCCTCAGATC'}, {'score': 2, 'version': 2L, 'sequence': 'TCGTATGGCACCCTCAGATC'})
({'score': 100, 'version': 0L, 'sequence': 'GACCCCCCCCAGTCCAACCC'}, {'score': 13, 'version': 2L, 'sequence': 'GACCCCCCCCAGTCCAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'GACCCCCCCCAGTCCAACCC'}, {'score': 13, 'version': 2L, 'sequence': 'GACCCCCCCCAGTCCAACCC'})
({'score': 100, 'version': 0L, 'sequence': 'GACCCCCCCCAGTCCAACCC'}, {'score': 13, 'version': 2L, 'sequence': 'GACCCCCCCCAGTCCAACCC'})
({'score': 0, 'version': 0L, 'sequence': 'TCCGGGGGCGGTGAGATCGC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCGGGGGCGGTGAGATCGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAGCTTTTGGCAGAGAGCC'}, {'score': 0, 'version': 2L, 'sequence': 'ATAGCTTTTGGCAGAGAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATAGCTTTTGGCAGAGAGCC'}, {'score': 0, 'version': 2L, 'sequence': 'ATAGCTTTTGGCAGAGAGCC'})
({'score': 66, 'version': 0L, 'sequence': 'ATTCGCTGCGAATCCATGCC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTCGCTGCGAATCCATGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTTCGGGGCCCCGAGCACC'}, {'score': 0, 'version': 2L, 'sequence': 'GGTTCGGGGCCCCGAGCACC'})
({'score': 0, 'version': 0L, 'sequence': 'ATTCGCTGCAAATCCATGCC'}, {'score': 41, 'version': 2L, 'sequence': 'ATTCGCTGCAAATCCATGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GGACTGGGCCGCCGAGACAC'}, {'score': 0, 'version': 2L, 'sequence': 'GGACTGGGCCGCCGAGACAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCAGGGAAGGGAGGGATTTC'}, {'score': 1, 'version': 2L, 'sequence': 'TCAGGGAAGGGAGGGATTTC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 96, 'version': 0L, 'sequence': 'ATCCCGGTGGGCCCCGGTTC'}, {'score': 10, 'version': 2L, 'sequence': 'ATCCCGGTGGGCCCCGGTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GATGCGGTCCCCGATCCCAC'}, {'score': 7, 'version': 2L, 'sequence': 'GATGCGGTCCCCGATCCCAC'})
({'score': 19, 'version': 0L, 'sequence': 'CTGAAGACACACAGAGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTGAAGACACACAGAGCTAC'})
({'score': 69, 'version': 0L, 'sequence': 'CCGTGACAAGTAGCTGTTAC'}, {'score': 5, 'version': 2L, 'sequence': 'CCGTGACAAGTAGCTGTTAC'})
({'score': 90, 'version': 0L, 'sequence': 'CCACAACAAGTAACTGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CCACAACAAGTAACTGCTAC'})
({'score': 26, 'version': 0L, 'sequence': 'ATCCCCATATGAGCTAAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCCCCATATGAGCTAAAAC'})
({'score': 26, 'version': 0L, 'sequence': 'ATCCCCATATGAGCTAAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCCCCATATGAGCTAAAAC'})
({'score': 26, 'version': 0L, 'sequence': 'ATCCCCATATGAGCTAAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCCCCATATGAGCTAAAAC'})
({'score': 26, 'version': 0L, 'sequence': 'ATCCCCATATGAGCTAAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCCCCATATGAGCTAAAAC'})
({'score': 26, 'version': 0L, 'sequence': 'ATCCCCATATGAGCTAAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCCCCATATGAGCTAAAAC'})
({'score': 26, 'version': 0L, 'sequence': 'ATCCCCATATGAGCTAAAAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCCCCATATGAGCTAAAAC'})
({'score': 0, 'version': 0L, 'sequence': 'CTGCTTTGTGGCTGAGATTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGCTTTGTGGCTGAGATTC'})
({'score': 97, 'version': 0L, 'sequence': 'AATGAATAAATAAGGATAGC'}, {'score': 100, 'version': 2L, 'sequence': 'AATGAATAAATAAGGATAGC'})
({'score': 99, 'version': 0L, 'sequence': 'TTTATTGCATTTATTATCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTATTGCATTTATTATCAC'})
({'score': 19, 'version': 0L, 'sequence': 'TGGCCTTCAAGTTGGACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGCCTTCAAGTTGGACTTC'})
({'score': 2, 'version': 0L, 'sequence': 'AGAATGGCCATTTGCTCTCC'}, {'score': 46, 'version': 2L, 'sequence': 'AGAATGGCCATTTGCTCTCC'})
({'score': 33, 'version': 0L, 'sequence': 'GCTGGACTTCTGGGTGTATC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTGGACTTCTGGGTGTATC'})
({'score': 15, 'version': 0L, 'sequence': 'AGAATGGCCATTTGCTCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAATGGCCATTTGCTCTAC'})
({'score': 17, 'version': 0L, 'sequence': 'TGGCCTTCAAGCTGGACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGCCTTCAAGCTGGACTTC'})
({'score': 15, 'version': 0L, 'sequence': 'AGAATGGCCATTTGCTCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAATGGCCATTTGCTCTAC'})
({'score': 17, 'version': 0L, 'sequence': 'TGGCCTTCAAGCTGGACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGCCTTCAAGCTGGACTTC'})
({'score': 2, 'version': 0L, 'sequence': 'AGAATGGCCATTTGCTCTCC'}, {'score': 46, 'version': 2L, 'sequence': 'AGAATGGCCATTTGCTCTCC'})
({'score': 17, 'version': 0L, 'sequence': 'TGGCCTTCAAGCTGGACTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGCCTTCAAGCTGGACTTC'})
({'score': 2, 'version': 0L, 'sequence': 'AGAATGGCCATTTGCTCTCC'}, {'score': 46, 'version': 2L, 'sequence': 'AGAATGGCCATTTGCTCTCC'})
({'score': 33, 'version': 0L, 'sequence': 'GCTGGACTTCTGGGTGTATC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTGGACTTCTGGGTGTATC'})
({'score': 97, 'version': 0L, 'sequence': 'TCGCCTTCATGCTGGGCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TCGCCTTCATGCTGGGCTTC'})
({'score': 15, 'version': 0L, 'sequence': 'AGAATGGCCATTTGCTCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAATGGCCATTTGCTCTAC'})
({'score': 43, 'version': 0L, 'sequence': 'GCTTGGATTTCAGGTGTATC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTTGGATTTCAGGTGTATC'})
({'score': 99, 'version': 0L, 'sequence': 'AGAAGAGCCATTTGCTCTAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAAGAGCCATTTGCTCTAC'})
({'score': 9, 'version': 0L, 'sequence': 'GCTGAGCTTTCGGGTGTATC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTGAGCTTTCGGGTGTATC'})
({'score': 95, 'version': 0L, 'sequence': 'ATAATTGTCATTTGCTCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'ATAATTGTCATTTGCTCTCC'})
({'score': 70, 'version': 0L, 'sequence': 'CTAAAAGATATATTGTCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTAAAAGATATATTGTCCTC'})
({'score': 2, 'version': 0L, 'sequence': 'TGGAGAGTGGGCCCATGGTC'}, {'score': 16, 'version': 2L, 'sequence': 'TGGAGAGTGGGCCCATGGTC'})
({'score': 63, 'version': 0L, 'sequence': 'CTGGGACCACCAGAAAACCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGGGACCACCAGAAAACCC'})
({'score': 12, 'version': 0L, 'sequence': 'TTAGAGGAAACCTATACCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTAGAGGAAACCTATACCCC'})
({'score': 1, 'version': 0L, 'sequence': 'AGAAGACGAACTTTGAAACC'}, {'score': 95, 'version': 2L, 'sequence': 'AGAAGACGAACTTTGAAACC'})
({'score': 88, 'version': 0L, 'sequence': 'CTGGATGATCCTCTTGCATC'}, {'score': 100, 'version': 2L, 'sequence': 'CTGGATGATCCTCTTGCATC'})
({'score': 99, 'version': 0L, 'sequence': 'TACAAATCATTCCAGTGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'TACAAATCATTCCAGTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAGGTAGAATCAGCCATCC'}, {'score': 98, 'version': 2L, 'sequence': 'AAAGGTAGAATCAGCCATCC'})
({'score': 78, 'version': 0L, 'sequence': 'TGGGGTAGGAGTTATGCAAC'}, {'score': 64, 'version': 2L, 'sequence': 'TGGGGTAGGAGTTATGCAAC'})
({'score': 96, 'version': 0L, 'sequence': 'AAGTAAATGGAGGTGTCAGC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGTAAATGGAGGTGTCAGC'})
({'score': 96, 'version': 0L, 'sequence': 'TCCATCTAACAAATCAATTC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCATCTAACAAATCAATTC'})
({'score': 65, 'version': 0L, 'sequence': 'TTTATACAGTAAATAACCCC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTATACAGTAAATAACCCC'})
({'score': 97, 'version': 0L, 'sequence': 'AGTTTGTATGAGAAAAGTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTTTGTATGAGAAAAGTTC'})
({'score': 18, 'version': 0L, 'sequence': 'AACATAGTTGTTCAGCTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AACATAGTTGTTCAGCTTTC'})
({'score': 22, 'version': 0L, 'sequence': 'TGGCAGAATGAAGTTTCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGCAGAATGAAGTTTCTTC'})
({'score': 96, 'version': 0L, 'sequence': 'TTCCAGGATGAAATTTCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TTCCAGGATGAAATTTCTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTCCAGGATGAGATTCTTC'}, {'score': 96, 'version': 2L, 'sequence': 'TTTCCAGGATGAGATTCTTC'})
({'score': 40, 'version': 0L, 'sequence': 'AACATAGTTGTTCAGCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AACATAGTTGTTCAGCTTCC'})
({'score': 92, 'version': 0L, 'sequence': 'TTTCAAAATGACATTTCTTC'}, {'score': 95, 'version': 2L, 'sequence': 'TTTCAAAATGACATTTCTTC'})
({'score': 90, 'version': 0L, 'sequence': 'AAAATAGTTGTTCAGCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'AAAATAGTTGTTCAGCTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGTTAAAGCCCCACTTACCC'}, {'score': 66, 'version': 2L, 'sequence': 'TGTTAAAGCCCCACTTACCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTATACTGATGCCTGGACTC'}, {'score': 96, 'version': 2L, 'sequence': 'TTATACTGATGCCTGGACTC'})
({'score': 83, 'version': 0L, 'sequence': 'CAAGAGAGATGGGTGGCTTC'}, {'score': 81, 'version': 2L, 'sequence': 'CAAGAGAGATGGGTGGCTTC'})
({'score': 25, 'version': 0L, 'sequence': 'ACTTGGGTTGTGTGATCCTC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTTGGGTTGTGTGATCCTC'})
({'score': 99, 'version': 0L, 'sequence': 'ACTCTATGAAACCTTAACTC'}, {'score': 59, 'version': 2L, 'sequence': 'ACTCTATGAAACCTTAACTC'})
({'score': 50, 'version': 0L, 'sequence': 'AATAAGCAGATAACTTGGTC'}, {'score': 100, 'version': 2L, 'sequence': 'AATAAGCAGATAACTTGGTC'})
({'score': 98, 'version': 0L, 'sequence': 'ATAAGAAATTCAGTCTTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'ATAAGAAATTCAGTCTTCTC'})
({'score': 98, 'version': 0L, 'sequence': 'AGCCCCTGTATGTTCACCTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCCCCTGTATGTTCACCTC'})
({'score': 99, 'version': 0L, 'sequence': 'ACCCTAAATTTTTCAAATCC'}, {'score': 100, 'version': 2L, 'sequence': 'ACCCTAAATTTTTCAAATCC'})
({'score': 66, 'version': 0L, 'sequence': 'ACAAGCCCTTGAAATTGATC'}, {'score': 100, 'version': 2L, 'sequence': 'ACAAGCCCTTGAAATTGATC'})
({'score': 66, 'version': 0L, 'sequence': 'ATGAAGTTTGGGTTTTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'ATGAAGTTTGGGTTTTGCAC'})
({'score': 66, 'version': 0L, 'sequence': 'CAGCGCGTTTCTCGCCGAGC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGCGCGTTTCTCGCCGAGC'})
({'score': 66, 'version': 0L, 'sequence': 'ATCATCGCAACCCTGTCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'ATCATCGCAACCCTGTCTTC'})
({'score': 66, 'version': 0L, 'sequence': 'AATATTAATCGCCCTTTGCC'}, {'score': 100, 'version': 2L, 'sequence': 'AATATTAATCGCCCTTTGCC'})
({'score': 66, 'version': 0L, 'sequence': 'ATTATTTATTTAGATTTCTC'}, {'score': 100, 'version': 2L, 'sequence': 'ATTATTTATTTAGATTTCTC'})
({'score': 65, 'version': 0L, 'sequence': 'TCCCATTCTTGTGATTTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCCCATTCTTGTGATTTAAC'})
({'score': 66, 'version': 0L, 'sequence': 'GGACACAAAACAACCTGATC'}, {'score': 100, 'version': 2L, 'sequence': 'GGACACAAAACAACCTGATC'})
({'score': 29, 'version': 0L, 'sequence': 'AGCTCCGTGTGTCTTCACCC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTCCGTGTGTCTTCACCC'})
({'score': 66, 'version': 0L, 'sequence': 'CTAAAACAGCTTATGTCTCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTAAAACAGCTTATGTCTCC'})
({'score': 66, 'version': 0L, 'sequence': 'CCTCCCAGAGGCTTGTGTCC'}, {'score': 56, 'version': 2L, 'sequence': 'CCTCCCAGAGGCTTGTGTCC'})
({'score': 66, 'version': 0L, 'sequence': 'AGCTTTATTACAGTAAATTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTACAGTAAATTC'})
({'score': 66, 'version': 0L, 'sequence': 'TTTATAACCATGGCAACAGC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTATAACCATGGCAACAGC'})
({'score': 45, 'version': 0L, 'sequence': 'TGTTAGGGTCCTATTTACTC'}, {'score': 42, 'version': 2L, 'sequence': 'TGTTAGGGTCCTATTTACTC'})
({'score': 0, 'version': 0L, 'sequence': 'ATTCGCTGCAAATCCATGCC'}, {'score': 41, 'version': 2L, 'sequence': 'ATTCGCTGCAAATCCATGCC'})
({'score': 0, 'version': 0L, 'sequence': 'TGATGAGCGAATTGTTTCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGATGAGCGAATTGTTTCAC'})
({'score': 0, 'version': 0L, 'sequence': 'TGAAACAATTCGCTCATCAC'}, {'score': 25, 'version': 2L, 'sequence': 'TGAAACAATTCGCTCATCAC'})
({'score': 90, 'version': 0L, 'sequence': 'ATAACATCCCTGTATTTATC'}, {'score': 100, 'version': 2L, 'sequence': 'ATAACATCCCTGTATTTATC'})
({'score': 0, 'version': 0L, 'sequence': 'AATAAAATTCGCGAAACGGC'}, {'score': 77, 'version': 2L, 'sequence': 'AATAAAATTCGCGAAACGGC'})
({'score': 12, 'version': 0L, 'sequence': 'TCGCCATTTCACGAATTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'TCGCCATTTCACGAATTTTC'})
({'score': 0, 'version': 0L, 'sequence': 'TGAAAAAATTTGCTCATCAC'}, {'score': 5, 'version': 2L, 'sequence': 'TGAAAAAATTTGCTCATCAC'})
({'score': 100, 'version': 0L, 'sequence': 'AAGAGAAGGGTTGCTCTCTC'}, {'score': 90, 'version': 2L, 'sequence': 'AAGAGAAGGGTTGCTCTCTC'})
({'score': 97, 'version': 0L, 'sequence': 'GTGGCAGGAGAATAGAGATC'}, {'score': 94, 'version': 2L, 'sequence': 'GTGGCAGGAGAATAGAGATC'})
({'score': 26, 'version': 0L, 'sequence': 'CGTCTTGGCCACCCCTGATC'}, {'score': 13, 'version': 2L, 'sequence': 'CGTCTTGGCCACCCCTGATC'})
({'score': 42, 'version': 0L, 'sequence': 'CGGTTATACCCCAGGTTATC'}, {'score': 100, 'version': 2L, 'sequence': 'CGGTTATACCCCAGGTTATC'})
({'score': 1, 'version': 0L, 'sequence': 'GAACCTCGGAAGTCAGGGAC'}, {'score': 100, 'version': 2L, 'sequence': 'GAACCTCGGAAGTCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCAAGGTTCTGTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCTTCCAAGGTTCTGTTCC'})
({'score': 15, 'version': 0L, 'sequence': 'AGAACCTTGGAAGGCAGGAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAACCTTGGAAGGCAGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 27, 'version': 0L, 'sequence': 'AGAACCTCGGAAGGCAGGAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAACCTCGGAAGGCAGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 32, 'version': 0L, 'sequence': 'CAGGGACTGGGAACAGAACC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGGGACTGGGAACAGAACC'})
({'score': 27, 'version': 0L, 'sequence': 'AGAACCTCGGAAGGCAGGAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAACCTCGGAAGGCAGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 27, 'version': 0L, 'sequence': 'AGAACCTCGGAAGGCAGGAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAACCTCGGAAGGCAGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCCGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTCGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCTGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCTGAGGTTCTGTTCC'})
({'score': 32, 'version': 0L, 'sequence': 'CAGGGACTGGGAACAGAACC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGGGACTGGGAACAGAACC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCCAAGGTTCTGTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCTTCCAAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTTGGAAGGCAGGGAC'}, {'score': 17, 'version': 2L, 'sequence': 'GAACCTTGGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCTGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCTGAGGTTCTGTTCC'})
({'score': 32, 'version': 0L, 'sequence': 'CAGGGACTGGGAACAGAACC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGGGACTGGGAACAGAACC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCTGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCTGAGGTTCTGTTCC'})
({'score': 32, 'version': 0L, 'sequence': 'CAGGGACTGGGAACAGAACC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGGGACTGGGAACAGAACC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCTGAGGTTCTGTTCC'}, {'score': 42, 'version': 2L, 'sequence': 'GCCTTCTGAGGTTCTGTTCC'})
({'score': 0, 'version': 0L, 'sequence': 'GAACCTCAGAAGGCAGGGAC'}, {'score': 100, 'version': 2L, 'sequence': 'GAACCTCAGAAGGCAGGGAC'})
({'score': 0, 'version': 0L, 'sequence': 'GCCTTCTAAGGTTCTGTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GCCTTCTAAGGTTCTGTTCC'})
({'score': 63, 'version': 0L, 'sequence': 'TTTATTCAGTGTTAATTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTATTCAGTGTTAATTAAC'})
({'score': 59, 'version': 0L, 'sequence': 'AGAATATTAAAAATTGTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'AGAATATTAAAAATTGTAAC'})
({'score': 99, 'version': 0L, 'sequence': 'CAGCACCTTGGACAGCTGAC'}, {'score': 100, 'version': 2L, 'sequence': 'CAGCACCTTGGACAGCTGAC'})
({'score': 90, 'version': 0L, 'sequence': 'GGCATTTTTTCCTGTTTAAC'}, {'score': 100, 'version': 2L, 'sequence': 'GGCATTTTTTCCTGTTTAAC'})
({'score': 95, 'version': 0L, 'sequence': 'GACAACTTATTGTTGTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'GACAACTTATTGTTGTGCAC'})
({'score': 10, 'version': 0L, 'sequence': 'TTTTCCAGCAATCTGGTTGC'}, {'score': 7, 'version': 2L, 'sequence': 'TTTTCCAGCAATCTGGTTGC'})
({'score': 0, 'version': 0L, 'sequence': 'AGTGACCCCCTTTTGAAAGC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTGACCCCCTTTTGAAAGC'})
({'score': 81, 'version': 0L, 'sequence': 'CTTCTTCTGCTAACATTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'CTTCTTCTGCTAACATTTTC'})
({'score': 54, 'version': 0L, 'sequence': 'AATAACAAAATAATGAAGAC'}, {'score': 100, 'version': 2L, 'sequence': 'AATAACAAAATAATGAAGAC'})
({'score': 22, 'version': 0L, 'sequence': 'AAGACAAATTGCAAAATTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AAGACAAATTGCAAAATTTC'})
({'score': 3, 'version': 0L, 'sequence': 'AACAGGTCCTATACCTGTAC'}, {'score': 100, 'version': 2L, 'sequence': 'AACAGGTCCTATACCTGTAC'})
({'score': 3, 'version': 0L, 'sequence': 'GCTCGGGACCTGGGGCCTTC'}, {'score': 100, 'version': 2L, 'sequence': 'GCTCGGGACCTGGGGCCTTC'})
({'score': 82, 'version': 0L, 'sequence': 'TGGTCTGTAGAGTAAAATAC'}, {'score': 100, 'version': 2L, 'sequence': 'TGGTCTGTAGAGTAAAATAC'})
({'score': 92, 'version': 0L, 'sequence': 'TAAATGTCTGAAATGTTATC'}, {'score': 100, 'version': 2L, 'sequence': 'TAAATGTCTGAAATGTTATC'})
({'score': 67, 'version': 0L, 'sequence': 'AAGGTTGAAAAACACTGGTC'}, {'score': 2, 'version': 2L, 'sequence': 'AAGGTTGAAAAACACTGGTC'})
({'score': 100, 'version': 0L, 'sequence': 'GTTATTCTCATATCTACCAC'}, {'score': 2, 'version': 2L, 'sequence': 'GTTATTCTCATATCTACCAC'})
({'score': 66, 'version': 0L, 'sequence': 'ATTCGCTGCGAATCCATGCC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTCGCTGCGAATCCATGCC'})
({'score': 0, 'version': 0L, 'sequence': 'GGGTTGGGGAATGCCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGTTGGGGAATGCCCTTCC'})
({'score': 2, 'version': 0L, 'sequence': 'AGGTATGGAGACCCCTTATC'}, {'score': 3, 'version': 2L, 'sequence': 'AGGTATGGAGACCCCTTATC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTTATGGATAATGGGTTTC'}, {'score': 0, 'version': 2L, 'sequence': 'CTTTATGGATAATGGGTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACACCACTGCCTGGTTGC'}, {'score': 0, 'version': 2L, 'sequence': 'TCACACCACTGCCTGGTTGC'})
({'score': 89, 'version': 0L, 'sequence': 'CAGGGACCGATATCGGCAGC'}, {'score': 0, 'version': 2L, 'sequence': 'CAGGGACCGATATCGGCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCCACCGGCAATTTACATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CCCACCGGCAATTTACATTC'})
({'score': 62, 'version': 0L, 'sequence': 'CTGCTTTATGGTTGAGATTC'}, {'score': 0, 'version': 2L, 'sequence': 'CTGCTTTATGGTTGAGATTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGAACATGTAAGTCGC'}, {'score': 93, 'version': 2L, 'sequence': 'GCGAACATGTAAGTCGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGGTTGTGGAATGCCCTTCC'}, {'score': 0, 'version': 2L, 'sequence': 'AGGTTGTGGAATGCCCTTCC'})
({'score': 1, 'version': 0L, 'sequence': 'CTAATATCTACAGTTAGTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATATCTACAGTTAGTAC'})
({'score': 1, 'version': 0L, 'sequence': 'TATATAACCTGCCTAACTGC'}, {'score': 52, 'version': 2L, 'sequence': 'TATATAACCTGCCTAACTGC'})
({'score': 96, 'version': 0L, 'sequence': 'ATTGTTTCAGCAGTCAGAAC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTGTTTCAGCAGTCAGAAC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 1, 'version': 0L, 'sequence': 'TAAACAGTAATTAAACCAAC'}, {'score': 100, 'version': 2L, 'sequence': 'TAAACAGTAATTAAACCAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGCACAGACCCGACCCTTGC'}, {'score': 8, 'version': 2L, 'sequence': 'GGCACAGACCCGACCCTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCTCTGTTATAAAGATAGC'}, {'score': 0, 'version': 2L, 'sequence': 'TGCTCTGTTATAAAGATAGC'})
({'score': 51, 'version': 0L, 'sequence': 'CCTATCCCATTCACACACAC'}, {'score': 1, 'version': 2L, 'sequence': 'CCTATCCCATTCACACACAC'})
({'score': 0, 'version': 0L, 'sequence': 'TTTAGCAGCTATTTGGTTGC'}, {'score': 21, 'version': 2L, 'sequence': 'TTTAGCAGCTATTTGGTTGC'})
({'score': 35, 'version': 0L, 'sequence': 'GGAGATTTGTCGTGGGCGAC'}, {'score': 1, 'version': 2L, 'sequence': 'GGAGATTTGTCGTGGGCGAC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 66, 'version': 0L, 'sequence': 'ATTCGCTGCGAATCCATGCC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTCGCTGCGAATCCATGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGCTACACAATTAGATGGC'}, {'score': 0, 'version': 2L, 'sequence': 'TGGCTACACAATTAGATGGC'})
({'score': 1, 'version': 0L, 'sequence': 'CTAATATCTACAGTTAGTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CTAATATCTACAGTTAGTAC'})
({'score': 95, 'version': 0L, 'sequence': 'ATAACTATATATACAACCAC'}, {'score': 2, 'version': 2L, 'sequence': 'ATAACTATATATACAACCAC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 0, 'version': 0L, 'sequence': 'CGCAATTGTGCTCTCTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'CGCAATTGTGCTCTCTGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAACAGTGAAAGGGCACAC'}, {'score': 6, 'version': 2L, 'sequence': 'CCAACAGTGAAAGGGCACAC'})
({'score': 13, 'version': 0L, 'sequence': 'ATGCCAGAGTGAAAGACAGC'}, {'score': 15, 'version': 2L, 'sequence': 'ATGCCAGAGTGAAAGACAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TATGAGCCTAAGTTCATAAC'}, {'score': 5, 'version': 2L, 'sequence': 'TATGAGCCTAAGTTCATAAC'})
({'score': 99, 'version': 0L, 'sequence': 'GTATTTATTTCTGTGTTTGC'}, {'score': 14, 'version': 2L, 'sequence': 'GTATTTATTTCTGTGTTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CCATGGGTATGGATATGGGC'}, {'score': 1, 'version': 2L, 'sequence': 'CCATGGGTATGGATATGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTATCTGCTCCAGCCATTTC'}, {'score': 95, 'version': 2L, 'sequence': 'CTATCTGCTCCAGCCATTTC'})
({'score': 99, 'version': 0L, 'sequence': 'TGTTTTTTTGGATCATACTC'}, {'score': 100, 'version': 2L, 'sequence': 'TGTTTTTTTGGATCATACTC'})
({'score': 62, 'version': 0L, 'sequence': 'ACAAAGCATAGAAAACAGGC'}, {'score': 99, 'version': 2L, 'sequence': 'ACAAAGCATAGAAAACAGGC'})
({'score': 93, 'version': 0L, 'sequence': 'GTTGAAACACCTGCCAAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'GTTGAAACACCTGCCAAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGCAGCTACATTGCCAGCTC'}, {'score': 97, 'version': 2L, 'sequence': 'AGCAGCTACATTGCCAGCTC'})
({'score': 100, 'version': 0L, 'sequence': 'TCACTCTATATCATTCCCTC'}, {'score': 99, 'version': 2L, 'sequence': 'TCACTCTATATCATTCCCTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCGTCGACTTACATTTTCAC'}, {'score': 59, 'version': 2L, 'sequence': 'CCGTCGACTTACATTTTCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TCCCCGATATGATATCCCAC'}, {'score': 0, 'version': 2L, 'sequence': 'TCCCCGATATGATATCCCAC'})
({'score': 98, 'version': 0L, 'sequence': 'CGATTGTGGTGAAATCAGTC'}, {'score': 100, 'version': 2L, 'sequence': 'CGATTGTGGTGAAATCAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'TATTGCTCAAAATAAATAAC'}, {'score': 99, 'version': 2L, 'sequence': 'TATTGCTCAAAATAAATAAC'})
({'score': 100, 'version': 0L, 'sequence': 'GGGTGAAAAGGCTGTGCAGC'}, {'score': 99, 'version': 2L, 'sequence': 'GGGTGAAAAGGCTGTGCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTATGGGAGATGACCTTCC'}, {'score': 6, 'version': 2L, 'sequence': 'TCTATGGGAGATGACCTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTATGGGAGATGACCTTCC'}, {'score': 6, 'version': 2L, 'sequence': 'TCTATGGGAGATGACCTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'TTTGGGCCACATGACTCTGC'}, {'score': 99, 'version': 2L, 'sequence': 'TTTGGGCCACATGACTCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAATAACAGATTAAGGACC'}, {'score': 93, 'version': 2L, 'sequence': 'AAAATAACAGATTAAGGACC'})
({'score': 100, 'version': 0L, 'sequence': 'AAAATAACAGATTAAGGACC'}, {'score': 93, 'version': 2L, 'sequence': 'AAAATAACAGATTAAGGACC'})
({'score': 99, 'version': 0L, 'sequence': 'CTTCCTTTTGGTTTCTGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTTCCTTTTGGTTTCTGGCC'})
({'score': 99, 'version': 0L, 'sequence': 'CTTCCTTTTGGTTTCTGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CTTCCTTTTGGTTTCTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'GACAATGAACAAAGGGTAAC'}, {'score': 90, 'version': 2L, 'sequence': 'GACAATGAACAAAGGGTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCACTGACCCCTGGGTCCAC'}, {'score': 1, 'version': 2L, 'sequence': 'CCACTGACCCCTGGGTCCAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGGTGCTGAACTTTCTCCTC'}, {'score': 98, 'version': 2L, 'sequence': 'TGGTGCTGAACTTTCTCCTC'})
({'score': 68, 'version': 0L, 'sequence': 'GTTTTTAGCAATAGACAGTC'}, {'score': 70, 'version': 2L, 'sequence': 'GTTTTTAGCAATAGACAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'CGGGTATGGGATCCTTTATC'}, {'score': 1, 'version': 2L, 'sequence': 'CGGGTATGGGATCCTTTATC'})
({'score': 99, 'version': 0L, 'sequence': 'TCAGTTTCATTAGATTGCAC'}, {'score': 100, 'version': 2L, 'sequence': 'TCAGTTTCATTAGATTGCAC'})
({'score': 100, 'version': 0L, 'sequence': 'GCTGGGGACCTGGGGTATGC'}, {'score': 97, 'version': 2L, 'sequence': 'GCTGGGGACCTGGGGTATGC'})
({'score': 100, 'version': 0L, 'sequence': 'CAGTAATTAATCCCAATAGC'}, {'score': 96, 'version': 2L, 'sequence': 'CAGTAATTAATCCCAATAGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTCTTTTTTGGTTTGGAAGC'}, {'score': 91, 'version': 2L, 'sequence': 'CTCTTTTTTGGTTTGGAAGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATACTCTTGAAGGGGAACTC'}, {'score': 95, 'version': 2L, 'sequence': 'ATACTCTTGAAGGGGAACTC'})
({'score': 100, 'version': 0L, 'sequence': 'TACATTTCAATTTATTTTGC'}, {'score': 94, 'version': 2L, 'sequence': 'TACATTTCAATTTATTTTGC'})
({'score': 98, 'version': 0L, 'sequence': 'GTCTGCTTCCTCTGTAGTTC'}, {'score': 13, 'version': 2L, 'sequence': 'GTCTGCTTCCTCTGTAGTTC'})
({'score': 0, 'version': 0L, 'sequence': 'GATTAAGAAGACCAACGGGC'}, {'score': 1, 'version': 2L, 'sequence': 'GATTAAGAAGACCAACGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TATGGCCCACGGGCCATGTC'}, {'score': 35, 'version': 2L, 'sequence': 'TATGGCCCACGGGCCATGTC'})
({'score': 100, 'version': 0L, 'sequence': 'TGATGTGGTCTCCGAACCCC'}, {'score': 43, 'version': 2L, 'sequence': 'TGATGTGGTCTCCGAACCCC'})
({'score': 100, 'version': 0L, 'sequence': 'TGATGTGGTCTCCGAACCCC'}, {'score': 43, 'version': 2L, 'sequence': 'TGATGTGGTCTCCGAACCCC'})
({'score': 100, 'version': 0L, 'sequence': 'GAATGACGGGTATAGGCGTC'}, {'score': 24, 'version': 2L, 'sequence': 'GAATGACGGGTATAGGCGTC'})
({'score': 92, 'version': 0L, 'sequence': 'TTCCTGCCCCACTGACAGCC'}, {'score': 93, 'version': 2L, 'sequence': 'TTCCTGCCCCACTGACAGCC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTGCACATTACCCAGCAGC'}, {'score': 99, 'version': 2L, 'sequence': 'ATTGCACATTACCCAGCAGC'})
({'score': 100, 'version': 0L, 'sequence': 'ATTATTATTTTCTATCTGTC'}, {'score': 50, 'version': 2L, 'sequence': 'ATTATTATTTTCTATCTGTC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGGGCCCTGGTGAAAAAC'}, {'score': 33, 'version': 2L, 'sequence': 'AGTGGGCCCTGGTGAAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGGGCCCTGGTGAAAAAC'}, {'score': 33, 'version': 2L, 'sequence': 'AGTGGGCCCTGGTGAAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGGGCCCTGGTGAAAAAC'}, {'score': 33, 'version': 2L, 'sequence': 'AGTGGGCCCTGGTGAAAAAC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTGGGCCCTGGTGAAAAAC'}, {'score': 33, 'version': 2L, 'sequence': 'AGTGGGCCCTGGTGAAAAAC'})
({'score': 99, 'version': 0L, 'sequence': 'GAAGCATTGGCGCTCTATCC'}, {'score': 66, 'version': 2L, 'sequence': 'GAAGCATTGGCGCTCTATCC'})
({'score': 99, 'version': 0L, 'sequence': 'GAAGCATTGGCGCTCTATCC'}, {'score': 66, 'version': 2L, 'sequence': 'GAAGCATTGGCGCTCTATCC'})
({'score': 99, 'version': 0L, 'sequence': 'GGCACCAGGGGTGGCAGACC'}, {'score': 66, 'version': 2L, 'sequence': 'GGCACCAGGGGTGGCAGACC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGGTGTGTGTAGGGTCCAC'}, {'score': 66, 'version': 2L, 'sequence': 'CTGGTGTGTGTAGGGTCCAC'})
({'score': 51, 'version': 0L, 'sequence': 'CACACCAGTCCAACCAAGTC'}, {'score': 66, 'version': 2L, 'sequence': 'CACACCAGTCCAACCAAGTC'})
({'score': 100, 'version': 0L, 'sequence': 'TTACTCTGGCGTCTGTCGGC'}, {'score': 66, 'version': 2L, 'sequence': 'TTACTCTGGCGTCTGTCGGC'})
({'score': 100, 'version': 0L, 'sequence': 'TTACATAATTGCATTATAAC'}, {'score': 63, 'version': 2L, 'sequence': 'TTACATAATTGCATTATAAC'})
({'score': 100, 'version': 0L, 'sequence': 'AATGCCTGAAATAAGACATC'}, {'score': 66, 'version': 2L, 'sequence': 'AATGCCTGAAATAAGACATC'})
({'score': 54, 'version': 0L, 'sequence': 'GCCTATTTTGAATCCCAATC'}, {'score': 1, 'version': 2L, 'sequence': 'GCCTATTTTGAATCCCAATC'})
({'score': 100, 'version': 0L, 'sequence': 'GGTTATAATGGGAAGTGTGC'}, {'score': 98, 'version': 2L, 'sequence': 'GGTTATAATGGGAAGTGTGC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTGCTCAATGTCTCCTTGC'}, {'score': 96, 'version': 2L, 'sequence': 'TCTGCTCAATGTCTCCTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CATCAGATGTTTTCATCTGC'}, {'score': 93, 'version': 2L, 'sequence': 'CATCAGATGTTTTCATCTGC'})
({'score': 100, 'version': 0L, 'sequence': 'GGAAAGCAGAACTAATTAAC'}, {'score': 97, 'version': 2L, 'sequence': 'GGAAAGCAGAACTAATTAAC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAATGTTATTCCAGTTACC'}, {'score': 97, 'version': 2L, 'sequence': 'CCAATGTTATTCCAGTTACC'})
({'score': 100, 'version': 0L, 'sequence': 'CCAATGTTATTCCAGTTACC'}, {'score': 97, 'version': 2L, 'sequence': 'CCAATGTTATTCCAGTTACC'})
({'score': 95, 'version': 0L, 'sequence': 'CGCCTGTTTGATAAATGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGCCTGTTTGATAAATGGCC'})
({'score': 95, 'version': 0L, 'sequence': 'CGCCTGTTTGATAAATGGCC'}, {'score': 100, 'version': 2L, 'sequence': 'CGCCTGTTTGATAAATGGCC'})
({'score': 11, 'version': 0L, 'sequence': 'GATTGGGGTGGATCAGGGGC'}, {'score': 25, 'version': 2L, 'sequence': 'GATTGGGGTGGATCAGGGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CGTTTTTATCTAGTTTTTCC'}, {'score': 88, 'version': 2L, 'sequence': 'CGTTTTTATCTAGTTTTTCC'})
({'score': 100, 'version': 0L, 'sequence': 'CGCACGTCCAAAAATTTTGC'}, {'score': 13, 'version': 2L, 'sequence': 'CGCACGTCCAAAAATTTTGC'})
({'score': 100, 'version': 0L, 'sequence': 'CGACTATTCCTTTTTGATGC'}, {'score': 0, 'version': 2L, 'sequence': 'CGACTATTCCTTTTTGATGC'})
({'score': 98, 'version': 0L, 'sequence': 'TTTGTCCCTCTCTGCTGGAC'}, {'score': 100, 'version': 2L, 'sequence': 'TTTGTCCCTCTCTGCTGGAC'})
({'score': 100, 'version': 0L, 'sequence': 'TGCCTGCTGTAGGGAATAGC'}, {'score': 99, 'version': 2L, 'sequence': 'TGCCTGCTGTAGGGAATAGC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTCACCGATAATATGCACC'}, {'score': 96, 'version': 2L, 'sequence': 'AGTCACCGATAATATGCACC'})
({'score': 100, 'version': 0L, 'sequence': 'AGTCACCGATAATATGCACC'}, {'score': 96, 'version': 2L, 'sequence': 'AGTCACCGATAATATGCACC'})
({'score': 99, 'version': 0L, 'sequence': 'ACAAGAAGCAGCTACTAATC'}, {'score': 100, 'version': 2L, 'sequence': 'ACAAGAAGCAGCTACTAATC'})
({'score': 94, 'version': 0L, 'sequence': 'AGTATTCTCTTACATAAGGC'}, {'score': 100, 'version': 2L, 'sequence': 'AGTATTCTCTTACATAAGGC'})
({'score': 100, 'version': 0L, 'sequence': 'CTGGGTGAAGACAATGTTTC'}, {'score': 97, 'version': 2L, 'sequence': 'CTGGGTGAAGACAATGTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'CCGCCTACCTTGCCTGCACC'}, {'score': 19, 'version': 2L, 'sequence': 'CCGCCTACCTTGCCTGCACC'})
({'score': 100, 'version': 0L, 'sequence': 'CCGCCTACCTTGCCTGCACC'}, {'score': 19, 'version': 2L, 'sequence': 'CCGCCTACCTTGCCTGCACC'})
({'score': 1, 'version': 0L, 'sequence': 'ATTACAATGCAGGATTCTGC'}, {'score': 100, 'version': 2L, 'sequence': 'ATTACAATGCAGGATTCTGC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 0, 'version': 0L, 'sequence': 'ACCGCTGTTCCGCGGCACAC'}, {'score': 99, 'version': 2L, 'sequence': 'ACCGCTGTTCCGCGGCACAC'})
({'score': 65, 'version': 0L, 'sequence': 'CAGGGGTGGGCCAAGCCGAC'}, {'score': 0, 'version': 2L, 'sequence': 'CAGGGGTGGGCCAAGCCGAC'})
({'score': 100, 'version': 0L, 'sequence': 'CTTTATGGATAATGGGTTTC'}, {'score': 0, 'version': 2L, 'sequence': 'CTTTATGGATAATGGGTTTC'})
({'score': 1, 'version': 0L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'}, {'score': 100, 'version': 2L, 'sequence': 'AGCTTTATTTGAGGGTTTTC'})
({'score': 100, 'version': 0L, 'sequence': 'GCGAACATGTAAGTCGC'}, {'score': 93, 'version': 2L, 'sequence': 'GCGAACATGTAAGTCGC'})
({'score': 0, 'version': 0L, 'sequence': 'ACTCACGGTGAGGCCTTACC'}, {'score': 100, 'version': 2L, 'sequence': 'ACTCACGGTGAGGCCTTACC'})
({'score': 0, 'version': 0L, 'sequence': 'GGGTTGGGGAATGCCCTTCC'}, {'score': 100, 'version': 2L, 'sequence': 'GGGTTGGGGAATGCCCTTCC'})
({'score': 90, 'version': 0L, 'sequence': 'CCACAACAAGTAACTGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'CCACAACAAGTAACTGCTAC'})
({'score': 67, 'version': 0L, 'sequence': 'GAGAAGACACACAGAGCTAC'}, {'score': 0, 'version': 2L, 'sequence': 'GAGAAGACACACAGAGCTAC'})
({'score': 17, 'version': 0L, 'sequence': 'TATTGGAGCCAAAACAATCC'}, {'score': 99, 'version': 2L, 'sequence': 'TATTGGAGCCAAAACAATCC'})
({'score': 16, 'version': 0L, 'sequence': 'ATACTGGCTGGAATCAAACC'}, {'score': 23, 'version': 2L, 'sequence': 'ATACTGGCTGGAATCAAACC'})
({'score': 0, 'version': 0L, 'sequence': 'CAAATTGAGGAATATTGGCC'}, {'score': 87, 'version': 2L, 'sequence': 'CAAATTGAGGAATATTGGCC'})
({'score': 100, 'version': 0L, 'sequence': 'TCTGTAACCTTGTTATGAGC'}, {'score': 6, 'version': 2L, 'sequence': 'TCTGTAACCTTGTTATGAGC'})
({'score': 17, 'version': 0L, 'sequence': 'AGAAATAGGGGTTGTAGCAC'}, {'score': 16, 'version': 2L, 'sequence': 'AGAAATAGGGGTTGTAGCAC'})
({'score': 66, 'version': 0L, 'sequence': 'ATTCGCTGCGAATCCATGCC'}, {'score': 0, 'version': 2L, 'sequence': 'ATTCGCTGCGAATCCATGCC'})
({'score': 77, 'version': 0L, 'sequence': 'TTGCCAAACAGAGCCTTCTC'}, {'score': 0, 'version': 2L, 'sequence': 'TTGCCAAACAGAGCCTTCTC'})
---------------------------------------------------------------------------
KeyboardInterrupt Traceback (most recent call last)
<ipython-input-72-cc98640ef640> in <module>()
1 for item in resultssorted:
----> 2 t = scoredblookup(item, genomename)
3 if len(t) ==2 and t[0]["score"] != t[1]["score"]:
4 print str(t) + "\n"
<ipython-input-71-14f1d38515e7> in scoredblookup(guide, genome)
3 cur = con.cursor(mdb.cursors.DictCursor)
4
----> 5 cur.execute("SELECT score, sequence, version FROM scores WHERE sequence='{0}' AND genome = '{1}'" .format(str(guide.seq), genome))
6 dbresult = cur.fetchall()
7 #guide.annotations["score"] = dbresult[0]["score"]
/Users/andylane/anaconda/lib/python2.7/site-packages/MySQLdb/cursors.pyc in execute(self, query, args)
188 try:
189 r = None
--> 190 r = self._query(query)
191 except TypeError, m:
192 if m.args[0] in ("not enough arguments for format string",
/Users/andylane/anaconda/lib/python2.7/site-packages/MySQLdb/cursors.pyc in _query(self, q)
352
353 def _query(self, q):
--> 354 rowcount = self._do_query(q)
355 self._post_get_result()
356 return rowcount
/Users/andylane/anaconda/lib/python2.7/site-packages/MySQLdb/cursors.pyc in _do_query(self, q)
316 db = self._get_db()
317 self._last_executed = q
--> 318 db.query(q)
319 self._do_get_result()
320 return self.rowcount
KeyboardInterrupt:
Traceback (most recent call last):
File "/Users/andylane/anaconda/lib/python2.7/site-packages/IPython/kernel/zmq/ipkernel.py", line 389, in execute_request
shell.run_cell(code, store_history=store_history, silent=silent)
File "/Users/andylane/anaconda/lib/python2.7/site-packages/IPython/core/interactiveshell.py", line 2743, in run_cell
self.events.trigger('post_execute')
File "/Users/andylane/anaconda/lib/python2.7/site-packages/IPython/core/events.py", line 82, in trigger
func(*args, **kwargs)
File "/Users/andylane/anaconda/lib/python2.7/site-packages/IPython/kernel/zmq/pylab/backend_inline.py", line 118, in flush_figures
return show(True)
File "/Users/andylane/anaconda/lib/python2.7/site-packages/IPython/kernel/zmq/pylab/backend_inline.py", line 47, in show
matplotlib.pyplot.close('all')
File "/Users/andylane/anaconda/lib/python2.7/site-packages/matplotlib/pyplot.py", line 511, in close
_pylab_helpers.Gcf.destroy_all()
File "/Users/andylane/anaconda/lib/python2.7/site-packages/matplotlib/_pylab_helpers.py", line 90, in destroy_all
gc.collect()
KeyboardInterrupt
In [121]:
len(t)
Out[121]:
1
In [234]:
for item in goodruns_filtered:
item.pullsequences()
In [232]:
# pickle.dump(goodruns_filtered, open("ch10 goodruns filtered to 1000.pkl", "wb"))
In [133]:
goodruns_filtered = pickle.load(open("ch10 goodruns filtered to 1000.pkl", "rb"))
In [10]:
import primer3
In [140]:
def al_primersearch(current_amp):
'''
Returns a dict of primer parameters.
'''
sequence_string = str(current_amp.permissible_region_masked.seq)
primeableregionleft_start = 0
primeableregionleft_length = current_amp.required_start_relative
primeableregionright_start = current_amp.required_end_relative
primeableregionright_length = len(current_amp.permissible_region)-current_amp.required_end_relative
seq_args = {"SEQUENCE_ID": current_amp.guides[0].id,
"SEQUENCE_TEMPLATE": sequence_string,
"SEQUENCE_PRIMER_PAIR_OK_REGION_LIST": [[primeableregionleft_start,primeableregionleft_length,
primeableregionright_start,primeableregionright_length]],
}
primerdict = primer3.bindings.designPrimers(seq_args, global_parameters)
primerdict["expandedpriming"] = 0
# If no primers are made, allow to prime 20 bp into the desired region:
if primerdict["PRIMER_PAIR_NUM_RETURNED"] == 0:
primeableregionleft_length = primeableregionleft_length + 20
primeableregionright_start = primeableregionright_start - 20
primeableregionright_length = len(current_amp.permissible_region)-current_amp.required_end_relative +20
seq_args = {"SEQUENCE_ID": current_amp.guides[0].id,
"SEQUENCE_TEMPLATE": sequence_string,
"SEQUENCE_PRIMER_PAIR_OK_REGION_LIST": [[primeableregionleft_start,primeableregionleft_length,
primeableregionright_start,primeableregionright_length]],
}
primerdict = primer3.bindings.designPrimers(seq_args, global_parameters)
primerdict["expandedpriming"] = 20
if primerdict["PRIMER_PAIR_NUM_RETURNED"] == 0:
primeableregionleft_length = primeableregionleft_length + 50
primeableregionright_start = primeableregionright_start - 50
primeableregionright_length = len(current_amp.permissible_region)-current_amp.required_end_relative +50
seq_args = {"SEQUENCE_ID": current_amp.guides[0].id,
"SEQUENCE_TEMPLATE": sequence_string,
"SEQUENCE_PRIMER_PAIR_OK_REGION_LIST": [[primeableregionleft_start,primeableregionleft_length,
primeableregionright_start,primeableregionright_length]],
}
primerdict = primer3.bindings.designPrimers(seq_args, global_parameters)
primerdict["expandedpriming"] = 50
return primerdict
In [141]:
def al_screen_primer(primer):
'''
Input is a primer as a string.
'''
currfile = open("currprimer.fa", "w")
currfile.write(">" + str(primer) + "\n")
currfile.write(str(primer))
currfile.close()
blastn_cline = NcbiblastnCommandline(query="currprimer.fa", db="xt80plus", \
task = "blastn-short",outfmt=5, out="primerblast.tmp", max_target_seqs=100, num_threads = 8, dust="no")
blastn_cline
result = blastn_cline()
badprimer = 0
# Parse data
result_handle = open("primerblast.tmp")
blast_record = NCBIXML.read(result_handle) # if there were multiple queries, use NCBIXML.parse(result_handle)
# How many matches are there with more than 14 or matching bases?
match14 = 0
for x in blast_record.alignments:
for y in x.hsps:
if y.positives > 14:
match14 = match14 + 1
match15 = 0
for x in blast_record.alignments:
for y in x.hsps:
if y.positives > 15:
match15 = match15 + 1
#print(primer.description)
#print(match14)
#print(match15)
# Set a cutoff of
if match14 > 40:
badprimer = 1
elif match15 > 30: #was 10
badprimer = 1
return badprimer
In [142]:
def al_collect_good_primers(template, primerdict):
i = 0
badlist = []
try:
#print(primerdict["PRIMER_PAIR_NUM_RETURNED"])
while i < primerdict["PRIMER_PAIR_NUM_RETURNED"]:
leftprimer = primerdict[str("PRIMER_LEFT_" + str(i) + "_SEQUENCE")]
leftprimer_start = str(primerdict[str("PRIMER_LEFT_"+ str(i))][0])
leftprimer_length = str(primerdict[str("PRIMER_LEFT_"+ str(i))][1])
leftprimer_gc = str(primerdict[str("PRIMER_LEFT_"+ str(i) + "_GC_PERCENT")])
leftprimer_tm = str(primerdict[str("PRIMER_LEFT_"+ str(i) + "_TM")])
rightprimer = primerdict[str("PRIMER_RIGHT_" + str(i) + "_SEQUENCE")]
rightprimer_start = str(primerdict[str("PRIMER_RIGHT_"+ str(i))][0])
rightprimer_length = str(primerdict[str("PRIMER_RIGHT_"+ str(i))][1])
rightprimer_gc = str(primerdict[str("PRIMER_RIGHT_"+ str(i) + "_GC_PERCENT")])
rightprimer_tm = str(primerdict[str("PRIMER_RIGHT_"+ str(i) + "_TM")])
product_len = int(rightprimer_start) - int(leftprimer_start) #rightprimer_start is already right edge of primer
left_bad = al_screen_primer(leftprimer)
right_bad = al_screen_primer(rightprimer)
if left_bad == 0 and right_bad == 0:
with open("primerlist.txt", "a") as primerlist:
primerlist.write("%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\n" % \
(template.guides[0].name,leftprimer,leftprimer_start,leftprimer_length,leftprimer_tm,leftprimer_gc,\
rightprimer,rightprimer_start,rightprimer_length,rightprimer_tm,rightprimer_gc,\
template.length,str(product_len),template.guidecount,str(primerdict["expandedpriming"])))
primerlist.close()
#print("Success!")
template.primers = {"name": template.guides[0].name,
"leftprimer": leftprimer,
"leftprimer_start": leftprimer_start,
"leftprimer_length": leftprimer_length,
"leftprimer_tm": leftprimer_tm,
"leftprimer_gc": leftprimer_gc,
"rightprimer": rightprimer,
"rightprimer_start": rightprimer_start,
"rightprimer_length": rightprimer_length,
"rightprimer_tm": rightprimer_tm,
"rightprimer_gc": rightprimer_gc,
"template_length": template.length,
"product_length": product_len,
"guidecount": template.guidecount,
"expandedpriming": primerdict["expandedpriming"]
}
break
if left_bad ==1:
None
#print("iteration" + str(i) + "left primer" + leftprimer + "is bad")
if right_bad == 1:
None
#print("iteration" + str(i) + "right primer" + rightprimer + "is bad")
if left_bad ==1 and right_bad ==1 and i ==primerdict["PRIMER_PAIR_NUM_RETURNED"]:
with open("primerlist.txt", "a") as primerlist:
primerlist.write("All the primers were bad for this amplicon!\n")
primerlist.close()
i = i +1
except:
with open("primerlist.txt", "a") as primerlist:
# primerlist.write("Primer3 failed to find any primers for this amplicon! " + primerdict["SEQUENCE_PRIMER_PAIR_OK_REGION_LIST"] + "\n")
primerlist.write("Primer3 failed to find any primers for this amplicon! " + "\n")
primerlist.close()
#print("Primer3 failed to find any primers for this amplicon! " + "\n")
print sys.exc_info()
In [143]:
global_parameters = {
'PRIMER_NUM_RETURN': 20,
'PRIMER_OPT_SIZE': 20,
'PRIMER_PICK_INTERNAL_OLIGO': 0,
'PRIMER_INTERNAL_MAX_SELF_END': 8,
'PRIMER_MIN_SIZE': 18,
'PRIMER_MAX_SIZE': 25,
'PRIMER_OPT_TM': 60.0,
'PRIMER_MIN_TM': 56.0,
'PRIMER_MAX_TM': 67.0,
'PRIMER_MIN_GC': 20.0,
'PRIMER_MAX_GC': 80.0,
'PRIMER_MAX_POLY_X': 100,
'PRIMER_INTERNAL_MAX_POLY_X': 100,
'PRIMER_SALT_MONOVALENT': 50.0,
'PRIMER_DNA_CONC': 50.0,
'PRIMER_MAX_NS_ACCEPTED': 0,
'PRIMER_MAX_SELF_ANY': 12,
'PRIMER_MAX_SELF_END': 8,
'PRIMER_WT_SELF_ANY': 1, # added
'PRIMER_PAIR_WT_COMPL_ANY':1, #added
'PRIMER_PAIR_WT_COMPL_END':1, #added
'PRIMER_PAIR_WT_SELF_END':1, #added
'PRIMER_PAIR_WT_SELF_ANY':1, #added
'PRIMER_PAIR_MAX_COMPL_ANY': 12,
'PRIMER_PAIR_MAX_COMPL_END': 8,
'PRIMER_PRODUCT_SIZE_RANGE': [100, 18000],
'PRIMER_MIN_THREE_PRIME_DISTANCE':3,
'PRIMER_PAIR_MAX_DIFF_TM':5, #added
'PRIMER_PAIR_WT_DIFF_TM':1, #added
}
In [136]:
import copy
In [137]:
for item in goodruns_filtered:
s = []
for c in list(item.permissible_region.seq):
if c.isupper():
s.append(c)
else:
s.append("N")
s = "".join(s)
item.permissible_region_masked = copy.copy(item.permissible_region)
item.permissible_region_masked.seq = Seq(s, IUPACAmbiguousDNA())
In [139]:
print goodruns_filtered[3].permissible_region_masked.seq
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACGATTAGTTCCAGTCCGATACATATTTATTATGGTGATATTAAACCTTTTAGAAGTCACTAAATTCAAGAGGTGAAAATAAGAGAGAGAAGCCTGGGAAACCACCCATACAGACACAGCTGAACAAGTGCAGTGAATCTGATATATATTACAAGCACAAAAACAGCATCACAATGGAACAGTTGTGGTGGCATTGGAATCCAGGGGTACAACAGAGATATGAGAGCCTTATACGCTACCAATCTAAGCATGAGTAACAAGGCTGAAACAGCCAGTCATATCAGAGCCATACACGGCATTTTTAGGCCATTGCTATTCCAAAAGGAACAACTGCCACTAAGTAGCAAATGTTTAGGGGAGCACAAAGTGAAGCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACCTGTACCTGATATAACTAATCTGTGTTTGGTTAATATTAAGTACTTGTCCTCTGTAATCGTGGGTGTATTTATCATGTAGGCCAACAGTTATTTTTTTATAGCCTTAACATAAATTTTGTTTAATTTTGACACCATTTTATATTTTCGGCTCAGGAATTTGTGGGAGGTGAGAGAGGATAGAGAAGAATATTTGAATGGCATTTCCCATAGGCTTTGGCAAATTATCTTTCTATAATTGTCCAGAATAGCTCAAGTTATCAATATACCCCATATAGCAAGTACTTCAAGGATTTCATATATGTACATGTTCCTCGTGAGCTAGATTTTGGTCAGTGCTTAAAGCAGCATTAGATCTGGGCATAAGTTNNNNNNNNNNNNNNNNNNNNNNCATGGACAGAGATCAAGTACAGCTAAAATATGAAATTTACATTAATGTAGTGTATTTTTCTGTTTAGCTAAAGGTACATGCTTTACACGCTAGGGCCAAATAGGGTTGATTTAACATGTTTAAGGTAATTCAGTTCTTCATTAGTCAAATGAGATAAGTTGTGCTTCGCTCCCTCCTGTTCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCACCTACAACTGGCATCTGAAAGACAGCGTTAGTTATTAGCCACTATACATATACAAACATCTAGACGCATAGATAGAAAACATACTAACCTGCTGGTACATGGGTTGCTGCCCACCAATGAATGGCTGCTGTTGAGAAGGTAGGTTGCTAAGAGATGGGTCCTGACCAGGCAGAGTAGACATAAGATTCTAAACAGTGGAAATATTGGTAGTAAATCTTTTTAAAAGTGACACTGATACATGAACACACATATTCTTAATGTGAATTAATCTCTCTGGTTGATTTTTTTTTTGATCATATCTATTTGTCTACCCAAAGATTATAAAATGCAAATGAATGAAGCTCATTGGTCATTGGTTGCTATTTTGTATTGATTAGACACGACTCCATATACGCCAGGGTTTGTACACCTTGGTTCAACATTTTATATGGTCAGTTTAGAACTTTTAACAGTTTTAAGCTGAGCAGCAAACTTTCTTTATCTAAAGACGAATATGGCTAGACTAAAGGCAAACTTATAACAGTAATCATTGAAGCCTTCAAAGTTGTGCTCAGTATAGCTCAATGGACCAGCTGGCTATTCTACAGATAAGCAATATTAAAACCCCTAAACCTTTAGTGTATATCTTACTGAACTATCCAATGATATGTAATATAGAACAGCCCCCAAGGCAGTTGTACACAAGGGAACCAGATTGAATAATACGTTGTTTTTCTTTATGTGGCCACCATTACTCCGAACCTTTACTAAAGAGCAATTTACCTGCATATTATATGGCTGATAAGGCATGGGGACAGACTGTGCACCCATGTAACCCACTGCACCCGCAGAAGCAGTCTGGGATATCGAGGGAAGGCTCTGAGATGCCACATTCTATAGGAAAAAAATAAAACATGCTCAAAGTCTGAACATTATTATGTGTGGCCCACTGCTCAAAACATGTAATTGTGCCTCTACACTAAAGTCGGTTTCCTATTAGATTGTAAGCTTTTGCCTGCACTGTTGTCATATTAAATTGTGTATCGCCAATACAGTCTTTTTCATACACTGCAGAACATATTCTGGCTTTATGAGTTAAAAGTTAACAAAAGACGAGCTTTAATTTAGGACTACAATACTGTATCAATTTCAATTATTTCAAATGTGCCGGTGTAGGGGGGGGCAAGGTAATATGGGTGTTTCAAATAATGCAATGAGATGCTAAAAATACTGTTACTGATCTTACAGTTACTAGTACAGTACACTATGCTTCTATAGGCCCCTCTTTTGTGTGGGAACATTTACTTTTTTGAACATTGCCCACAACACCAGTTCTGGCTCCATTCTTACTCACTCAACACAGTACCCAACACTTAAACCTATTAAATATGGCAGACTACTTTACATGCTCAGAATCATCCCCTCTGATTACCTGGTAGGCTTGAGTAGGTGTGGGCTGATAAGACGTGTATGTTGGGTTGGTGGTTGGTACAGCCTGCCCCTGCTGAGCTGTGGGAGCACATCCTGCTGTGTACATATATGCATTCACCATGTTGGGATCTGTGAGAAAAACACGGTTTTGATTAGGTCTAACATATAAGCAGGATTTCCAACATCCAGTCAAGCACCTTGACATCTCACCTTGAACCTGCATGGCAGGGTATGGCGCTGTGGCTGGCTGTCCTGATGGGTTCATATACACACCATGCATTGGGGAACCCTCCACAGAACCAGATGGGCTAAAGGTACCAGGGTAGGCAGGGGGTCCAGAGGGTGGGTACATAATGCCCCCAGTCGCAGGTAGTGATTGTAGCTGGAGGGTATAACATCATAATTTCAGTTAGTAACTGGCAGTGTTTCTGAAATCTTTGAAAAAAAAAAAAGATGGTGGACAAACCTGTGCATATGGGAGGGAGAAAGCAGGCATCTGGGCTCTCATCTGAATTGTCTGTTTCTGCTGCTCCAGTCGCAATTGTCTCTCCTTTTCCTGCTCCTGCAAACGTTGGATTGCCAGCTGGCGTTGCATCTCCAGGTATTCCTAGGGATTAAAACCAGGCTGACATTATAATGGACCTGCCAACATTATTACTCCTCACTAAAAAAATAAATAAGTGTATTAAGTGACAGGAGGGAGAATCCAATAACTGCTTCCCACACTCACATCCTAAGGAAATCCACCCCTAATAGCATTAGCATTAATCAATTACATGCCACATTCCCCCCACACTGTGTTTAGCAGTTTATCTGCATCACCTGCTTTTTTTGTCTCATAATCTCCAGTTTTTGCGCCAATTGGATTTGCCGTTGCCTCTCAGCCTCCTCTGCTGCACGCCGGAGCTTTTCCCTGTGCTCATCACGAAGAGCATTCAGTGCTCCTCGCGCATCCCGAATTTGTGCAAGTTTGTCTTGCAGGCCCTCGTAGTACACTAATGGGAGAAAAATTAGGTTAAAAGGAAAGAAGCATGATTAAATAATGTTTTTTTAAGAAGGTACTTCTTTGTATTACCAATATTCCTGGAAAAAGCAGCACAATTACTAGCTGTTATATGATTATGGAGTTTTTCTTTCAATAAAATGCTGCAACAAAGACTGTTGCTTCACTAAGTCTGGCACACCTTTTTTATATCAAAGGCTAGAGGAATTTTTAAAACCAAGTGACTGAGACTGTTCGCACATTAAGTAAAATAATGGCTCTTTCCCACCCGCTCCTAAATCTACACATTGTAAACACTTACATCGCCTTTCATCCAACTGATTGAGCATCTCTAGCAACTGTGGGTGCATGTTGTTAATGGACTGGAAGAGGGATAATACAGCAGAGTCATTCGTGATGCTCCTGCCACGCATTTGGTTGCTTTTCATACGGTTCAAAAATGTGGTAACTGCATTCTGCAAAGCTTTTAGGAACTGCTCGTGATTCTCCTCTGACTCCCCATTCTGGTATTGCTGCTGAAAACAACGGAAGTTAGACCAACATGAAAAGGCTTTAGGGTCCTACATGTGCATCAACAGGACACGTGAACTAAACTGACATGTAGAAGCAACAAAGCAGTCTAAATCTTCTTGTCCAAAGCATTTTGATTTAGTTAAATCTCATTCGTGTATGCGGATGATAAAAACACAAAAATAATCAGGTACTGAAATATTACATAACTGGGACCATCAGTTAACCAGGAACAGACTAATGGCCAGTCAGCAAAAGGAAATTTAACTGGATGTTTGCATAGATTCCATGGACTGTTTAGCTGCCTGGCTAAAACAAGTGGGGTAACAAAAGGTAACAGCCATTGAGCTGCCTACCAGTTAAATAAAGCAAATGGCCTTTTGAAGGGGAAAACTTAAGAATATGTTTCAACAACAGAAGAGGGTGATTTGGGAAATAGTGTATGGATAGCTACATATAACAAAAATACTAAACTGTTTTTGGTAACGTGCAATGCCAGCACTCACCATAATGTTAATCCCTTCCCCCTAGGCTAATGGTCAGGACCTGCCACCAACACTTATATATTTTAGCCCTAACCTTAGCCGTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAAATCGAATGTGCTTTTTTAATAGGCTGAGAAGGCCTGTAGGAAAAGGAAGACATTGGTGAAATGATAGGAGGTGTTAAAACATAAAAACAGTGTTTGTGGGAGAGTCTGTCCTTTGGTCTGGAGGCTTAAGCTGCCATGGGCGACTAGGAAACTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCAGAGTAAATTATACCTTATGTTTCATTAAATCTAAAGTCCAGTATTAATCTCAGGCTAAAACAATTCTATATGCCCTCATGATGCAGTGGGACATAAATGTTTCTTCTTAATAATAAAGATGAAAAAAGAAAAAATGCTGTTAAATCCCTTGGGCTAATGAAATATGCTTTCTAGCAGTTATTCCCCATTGTTTTAAGAGATAATCCTATTGCTCATGATAGAAACTAGCATATTCAGTGTGCTATCTGATGTGATCCCTACTTGCCTGTACTAGCACCTGTCACAGGCAAAGCATTCCTTTTTCAAATAAATTCAAGCTAGTGCCAGAGGGTAAGGCCTCTTTGATTGTTGTGGGTCTCTCTGAATGGACTGTAATCAAAATAATGAGACTGCCATAAGCCTTAAAAAGGCAACCCCCCCACCCACCCCATCTTTGTAATATTAAGCAGAAGTGAACAACAGCAGTCACCCAATAAACTTTACACTTACCTCCATGACTGTCACTGGCACATCACCTCCCTGAAGAACAGCAGGCTCACTGATTGGGACAGGGGCAGAGGGTGTAGGGCTTTTCCTGACCTCTTCCTGCTTCTTCTCCCAGTAATTACGATTCAGGTAACGAGCTAGCTACAGGGGA
In [19]:
import primer3
In [144]:
p = ProgressBar(len(goodruns_filtered))
with open("primerlist.txt", "w") as primerlist:
primerlist.write("Sequence_id\tforward_seq\tforward_start\tforward_length\tforward_tm\tforward_gc\treverse_seq\treverse_start\treverse_length\treverse_tm\treverse_gc\tinput_seq_length\tPCR_product_length\tGuides_Contained\n")
primerlist.close()
i = 0
for item in goodruns_filtered:
if "N" * 20 not in str(item.permissible_region.seq):
current_amp = item
primerdict = al_primersearch(current_amp)
al_collect_good_primers(item, primerdict)
i = i+1
p.animate(i)
[**********************95%********************* ] 854 of 898 complete
In [145]:
goodruns_filtered[3].permissible_region_masked.seq
Out[145]:
Seq('NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN...GGA', IUPACAmbiguousDNA())
In [146]:
pickle.dump(goodruns_filtered, open("goodruns_filtered_20150304_1247_nremoved.pkl", "wb"))
In [134]:
# goodruns_filtered = pickle.load(open("goodruns_filtered_20150302_1715.pkl", "rb"))
In [147]:
goodruns_filtered_with_primers = []
for item in goodruns_filtered:
try:
print item.primers
goodruns_filtered_with_primers.append(item)
except:
None
{'rightprimer': 'ACAGTTCTAGTTGGTTGGCCA', 'rightprimer_start': '5652', 'rightprimer_tm': '59.506977668', 'leftprimer_length': '20', 'template_length': 5782, 'leftprimer_gc': '60.0', 'leftprimer_start': '9', 'name': '31146560', 'guidecount': 65, 'leftprimer_tm': '59.8846505619', 'product_length': 5643, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'TAGAGGCCCTCAGGATAGCC', 'expandedpriming': 20}
{'rightprimer': 'GAACAGCTAGGGAATGGGGT', 'rightprimer_start': '4767', 'rightprimer_tm': '59.0802900455', 'leftprimer_length': '18', 'template_length': 4763, 'leftprimer_gc': '61.1111111111', 'leftprimer_start': '1', 'name': '16718988', 'guidecount': 62, 'leftprimer_tm': '57.3742004186', 'product_length': 4766, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCACACTAGGCTGAAGGC', 'expandedpriming': 0}
{'rightprimer': 'TCCCCTGTAGCTAGCTCGTT', 'rightprimer_start': '6232', 'rightprimer_tm': '60.0334045335', 'leftprimer_length': '23', 'template_length': 6224, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '337', 'name': '17468389', 'guidecount': 59, 'leftprimer_tm': '59.0502462353', 'product_length': 5895, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACGATTAGTTCCAGTCCGATACA', 'expandedpriming': 0}
{'rightprimer': 'AGCAGCTTGACCATGGAGTT', 'rightprimer_start': '4450', 'rightprimer_tm': '59.5967290361', 'leftprimer_length': '20', 'template_length': 4514, 'leftprimer_gc': '60.0', 'leftprimer_start': '12', 'name': '8382544', 'guidecount': 52, 'leftprimer_tm': '59.8285454491', 'product_length': 4438, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CACTCTACCGGTCTGCAGTC', 'expandedpriming': 50}
{'rightprimer': 'TGCAACTCTGGGTTTGGTGT', 'rightprimer_start': '4063', 'rightprimer_tm': '60.0336006873', 'leftprimer_length': '20', 'template_length': 4175, 'leftprimer_gc': '55.0', 'leftprimer_start': '370', 'name': '22197396', 'guidecount': 52, 'leftprimer_tm': '60.0337963284', 'product_length': 3693, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACATGGACTTGGGCCTCATG', 'expandedpriming': 0}
{'rightprimer': 'TCCAATAGCTCAGCTGGCAG', 'rightprimer_start': '5182', 'rightprimer_tm': '59.8202982265', 'leftprimer_length': '24', 'template_length': 5249, 'leftprimer_gc': '45.8333333333', 'leftprimer_start': '36', 'name': '24333768', 'guidecount': 50, 'leftprimer_tm': '60.0174067439', 'product_length': 5146, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACCCAGTGGACTATGACTAAGAGA', 'expandedpriming': 0}
{'rightprimer': 'AACAGCTTAGATGAGCCGGG', 'rightprimer_start': '3411', 'rightprimer_tm': '59.8210001113', 'leftprimer_length': '20', 'template_length': 3450, 'leftprimer_gc': '55.0', 'leftprimer_start': '39', 'name': '10386209', 'guidecount': 49, 'leftprimer_tm': '59.9664959144', 'product_length': 3372, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCACTTTGCAGACGACCCTC', 'expandedpriming': 50}
{'rightprimer': 'TATATAGCCCACTGCCCCCA', 'rightprimer_start': '3077', 'rightprimer_tm': '59.8051584012', 'leftprimer_length': '19', 'template_length': 3072, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '0', 'name': '18167375', 'guidecount': 49, 'leftprimer_tm': '60.0003958935', 'product_length': 3077, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCTGGGCTGGAAGTGAAT', 'expandedpriming': 0}
{'rightprimer': 'GCCTTCCCCTGGCTGTATTA', 'rightprimer_start': '3270', 'rightprimer_tm': '59.1560161003', 'leftprimer_length': '24', 'template_length': 3510, 'leftprimer_gc': '37.5', 'leftprimer_start': '454', 'name': '6750168', 'guidecount': 48, 'leftprimer_tm': '58.7364013971', 'product_length': 2816, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AAACCAGGCTGAATATCAATGACA', 'expandedpriming': 20}
{'rightprimer': 'GGCACCAGGTATAGCATTACAGT', 'rightprimer_start': '3161', 'rightprimer_tm': '60.1814541626', 'leftprimer_length': '20', 'template_length': 3144, 'leftprimer_gc': '60.0', 'leftprimer_start': '80', 'name': '6894719', 'guidecount': 47, 'leftprimer_tm': '60.2500382702', 'product_length': 3081, 'rightprimer_length': '23', 'rightprimer_gc': '47.8260869565', 'leftprimer': 'CACGGAGATGCACCTATGGG', 'expandedpriming': 20}
{'rightprimer': 'CTCCTGGCCTCCTCATGTAAG', 'rightprimer_start': '2470', 'rightprimer_tm': '59.5843409554', 'leftprimer_length': '20', 'template_length': 2553, 'leftprimer_gc': '50.0', 'leftprimer_start': '111', 'name': '559873', 'guidecount': 46, 'leftprimer_tm': '59.5175537345', 'product_length': 2359, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'AAGTCCTGGACAAATGCCCA', 'expandedpriming': 20}
{'rightprimer': 'TCTCCTGAGGGGGCAATACA', 'rightprimer_start': '2454', 'rightprimer_tm': '59.9559080083', 'leftprimer_length': '20', 'template_length': 2537, 'leftprimer_gc': '55.0', 'leftprimer_start': '254', 'name': '3097982', 'guidecount': 46, 'leftprimer_tm': '59.9631254283', 'product_length': 2200, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGCTTAAGTGCCCCTGTCAC', 'expandedpriming': 0}
{'rightprimer': 'GTGGAAGGTGTGGGTCCATT', 'rightprimer_start': '3261', 'rightprimer_tm': '59.8875877447', 'leftprimer_length': '24', 'template_length': 3482, 'leftprimer_gc': '45.8333333333', 'leftprimer_start': '31', 'name': '13908473', 'guidecount': 46, 'leftprimer_tm': '59.8427114835', 'product_length': 3230, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTTCCTAGCTACTCTTACACGTGG', 'expandedpriming': 20}
{'rightprimer': 'TTCTCTTTGCCGGTCTCGTC', 'rightprimer_start': '2399', 'rightprimer_tm': '60.0385264564', 'leftprimer_length': '20', 'template_length': 2392, 'leftprimer_gc': '55.0', 'leftprimer_start': '4', 'name': '33538787', 'guidecount': 46, 'leftprimer_tm': '60.4712114147', 'product_length': 2395, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTCAGGAGACCGGACCAACT', 'expandedpriming': 0}
{'rightprimer': 'GACTTTGCCAACCTTGTGGG', 'rightprimer_start': '3104', 'rightprimer_tm': '59.6109753153', 'leftprimer_length': '20', 'template_length': 3124, 'leftprimer_gc': '55.0', 'leftprimer_start': '17', 'name': '37817539', 'guidecount': 46, 'leftprimer_tm': '59.6653235453', 'product_length': 3087, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAGGGGTTGCCCTAATGGAA', 'expandedpriming': 20}
{'rightprimer': 'GGAAAGTTGCCTGGCGGA', 'rightprimer_start': '4666', 'rightprimer_tm': '60.2821803615', 'leftprimer_length': '20', 'template_length': 4658, 'leftprimer_gc': '55.0', 'leftprimer_start': '0', 'name': '29345395', 'guidecount': 45, 'leftprimer_tm': '58.8398634879', 'product_length': 4666, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'GGAACATCACACTCACAGCC', 'expandedpriming': 0}
{'rightprimer': 'TCCAGCCTCATCACAGCAAC', 'rightprimer_start': '3103', 'rightprimer_tm': '60.3219192968', 'leftprimer_length': '20', 'template_length': 3297, 'leftprimer_gc': '55.0', 'leftprimer_start': '384', 'name': '2866565', 'guidecount': 44, 'leftprimer_tm': '60.3920598992', 'product_length': 2719, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAGCCAGCACACTCTATGCA', 'expandedpriming': 0}
{'rightprimer': 'ATAAATACAACCCCCGCCCC', 'rightprimer_start': '2212', 'rightprimer_tm': '59.8133704708', 'leftprimer_length': '21', 'template_length': 2270, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '890', 'name': '5122384', 'guidecount': 44, 'leftprimer_tm': '59.7858250042', 'product_length': 1322, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCACTTTTGCCTGTGGGAGAA', 'expandedpriming': 0}
{'rightprimer': 'GACCCTCCAAACCTTCGGTC', 'rightprimer_start': '1662', 'rightprimer_tm': '60.3213867358', 'leftprimer_length': '18', 'template_length': 1653, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '12', 'name': '18171250', 'guidecount': 44, 'leftprimer_tm': '60.2030147353', 'product_length': 1650, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CTCATTGGCCCCCGTACG', 'expandedpriming': 20}
{'rightprimer': 'GTCTGGACACACGGCACTAG', 'rightprimer_start': '2594', 'rightprimer_tm': '60.3886489486', 'leftprimer_length': '20', 'template_length': 2629, 'leftprimer_gc': '55.0', 'leftprimer_start': '5', 'name': '1157455', 'guidecount': 43, 'leftprimer_tm': '60.0344770646', 'product_length': 2589, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'ACACCGGGGGCTTAAATCTG', 'expandedpriming': 20}
{'rightprimer': 'AGTCTGGTGTTGTTGCTTAGGT', 'rightprimer_start': '3287', 'rightprimer_tm': '59.8272825472', 'leftprimer_length': '22', 'template_length': 3397, 'leftprimer_gc': '50.0', 'leftprimer_start': '1', 'name': '2381735', 'guidecount': 43, 'leftprimer_tm': '59.4283469622', 'product_length': 3286, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'TGGACACCTGGAAGTTATCAGG', 'expandedpriming': 0}
{'rightprimer': 'ATCCGGGAGCTGAGGGTAAT', 'rightprimer_start': '2536', 'rightprimer_tm': '60.1049342064', 'leftprimer_length': '19', 'template_length': 2713, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '28', 'name': '6108835', 'guidecount': 43, 'leftprimer_tm': '59.7797944486', 'product_length': 2508, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTAGGCACAGGCTCCACAG', 'expandedpriming': 20}
{'rightprimer': 'CGCTGGCCTGGCTTTTGG', 'rightprimer_start': '3047', 'rightprimer_tm': '62.0600381144', 'leftprimer_length': '19', 'template_length': 3042, 'leftprimer_gc': '68.4210526316', 'leftprimer_start': '0', 'name': '32890558', 'guidecount': 43, 'leftprimer_tm': '61.7357719233', 'product_length': 3047, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'CACCCATCTCTCCCCGGTG', 'expandedpriming': 0}
{'rightprimer': 'TGTATGTCTCCGGCATGTCTG', 'rightprimer_start': '1997', 'rightprimer_tm': '59.8631093626', 'leftprimer_length': '20', 'template_length': 1990, 'leftprimer_gc': '55.0', 'leftprimer_start': '251', 'name': '33834662', 'guidecount': 43, 'leftprimer_tm': '59.9559080083', 'product_length': 1746, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'TGGGGGCTCTACATCCTTCA', 'expandedpriming': 0}
{'rightprimer': 'CAAGCAGCAGAGAGACGGAG', 'rightprimer_start': '4238', 'rightprimer_tm': '60.4581974163', 'leftprimer_length': '20', 'template_length': 4241, 'leftprimer_gc': '60.0', 'leftprimer_start': '9', 'name': '35055252', 'guidecount': 43, 'leftprimer_tm': '60.4620473311', 'product_length': 4229, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCTGGGGGTTCGATTCTTCG', 'expandedpriming': 0}
{'rightprimer': 'GCACATGTGTTACAGCCTGC', 'rightprimer_start': '3922', 'rightprimer_tm': '60.1095968673', 'leftprimer_length': '20', 'template_length': 4013, 'leftprimer_gc': '55.0', 'leftprimer_start': '1', 'name': '6669609', 'guidecount': 42, 'leftprimer_tm': '60.3231780629', 'product_length': 3921, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCTGCCTCTCAAGCACCAAT', 'expandedpriming': 0}
{'rightprimer': 'GCAAAAGGTAGAAACTACACTCACT', 'rightprimer_start': '3695', 'rightprimer_tm': '59.241215833', 'leftprimer_length': '20', 'template_length': 3740, 'leftprimer_gc': '55.0', 'leftprimer_start': '73', 'name': '7345913', 'guidecount': 42, 'leftprimer_tm': '59.4049050896', 'product_length': 3622, 'rightprimer_length': '25', 'rightprimer_gc': '40.0', 'leftprimer': 'GGAAGTGACTGCCAGCTTTG', 'expandedpriming': 50}
{'rightprimer': 'TAAGGGAGGGCACACAGTAC', 'rightprimer_start': '5840', 'rightprimer_tm': '58.7271932056', 'leftprimer_length': '25', 'template_length': 5928, 'leftprimer_gc': '36.0', 'leftprimer_start': '142', 'name': '16701176', 'guidecount': 42, 'leftprimer_tm': '58.5776669315', 'product_length': 5698, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGTATCTTCATGATGCCCTGTTAAA', 'expandedpriming': 0}
{'rightprimer': 'ACACCAGGGTTTACAGGATGTC', 'rightprimer_start': '4986', 'rightprimer_tm': '59.9601214287', 'leftprimer_length': '21', 'template_length': 5094, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '45', 'name': '24137514', 'guidecount': 42, 'leftprimer_tm': '59.7169705392', 'product_length': 4941, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'ACAGAATGACCAGGGATGTGG', 'expandedpriming': 20}
{'rightprimer': 'CCCCACAATCCTTGCTCTGT', 'rightprimer_start': '2272', 'rightprimer_tm': '59.9612556273', 'leftprimer_length': '21', 'template_length': 2323, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '50', 'name': '29148993', 'guidecount': 42, 'leftprimer_tm': '60.0644628227', 'product_length': 2222, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCATGAAGACAATGGAGGGA', 'expandedpriming': 50}
{'rightprimer': 'ATGGTCAGACCGGCCTATCG', 'rightprimer_start': '2697', 'rightprimer_tm': '61.4643759288', 'leftprimer_length': '18', 'template_length': 2699, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '0', 'name': '1464472', 'guidecount': 41, 'leftprimer_tm': '61.6372729252', 'product_length': 2697, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'AAGCAGACCGGGTCACCC', 'expandedpriming': 0}
{'rightprimer': 'TTCTGTAGGACTAGTGTAGATGCA', 'rightprimer_start': '2961', 'rightprimer_tm': '58.4932917369', 'leftprimer_length': '23', 'template_length': 2962, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '16', 'name': '3801630', 'guidecount': 41, 'leftprimer_tm': '58.7928353907', 'product_length': 2945, 'rightprimer_length': '24', 'rightprimer_gc': '41.6666666667', 'leftprimer': 'GCTCAAAGCTATGGGGAATTACA', 'expandedpriming': 20}
{'rightprimer': 'GAGCACCTTCGCCGGACC', 'rightprimer_start': '3042', 'rightprimer_tm': '63.1201779194', 'leftprimer_length': '20', 'template_length': 3036, 'leftprimer_gc': '60.0', 'leftprimer_start': '102', 'name': '2241619', 'guidecount': 40, 'leftprimer_tm': '60.3228160882', 'product_length': 2940, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'CTGGAGTTCCACCCACATGG', 'expandedpriming': 20}
{'rightprimer': 'CAAATGCAGTGCCGGGTATG', 'rightprimer_start': '5184', 'rightprimer_tm': '59.8989600414', 'leftprimer_length': '20', 'template_length': 5241, 'leftprimer_gc': '55.0', 'leftprimer_start': '139', 'name': '5992842', 'guidecount': 40, 'leftprimer_tm': '59.6763710045', 'product_length': 5045, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTTTAGACCTGGTCCCTGCG', 'expandedpriming': 20}
{'rightprimer': 'GGTTCTCAAATAACCGACCCAGA', 'rightprimer_start': '2858', 'rightprimer_tm': '60.3071989053', 'leftprimer_length': '20', 'template_length': 2863, 'leftprimer_gc': '60.0', 'leftprimer_start': '30', 'name': '7122179', 'guidecount': 40, 'leftprimer_tm': '60.1069674403', 'product_length': 2828, 'rightprimer_length': '23', 'rightprimer_gc': '47.8260869565', 'leftprimer': 'GAATCCTCTTGTCCCTGGCC', 'expandedpriming': 20}
{'rightprimer': 'CGTTTACCTGCCCCATTTGG', 'rightprimer_start': '3320', 'rightprimer_tm': '59.469711984', 'leftprimer_length': '20', 'template_length': 3469, 'leftprimer_gc': '55.0', 'leftprimer_start': '1', 'name': '9344062', 'guidecount': 40, 'leftprimer_tm': '59.456723162', 'product_length': 3319, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTGCACTGAGGCTAATGGGA', 'expandedpriming': 0}
{'rightprimer': 'CCCTCACTTTATCAGCGGGG', 'rightprimer_start': '2544', 'rightprimer_tm': '60.1790454195', 'leftprimer_length': '20', 'template_length': 2546, 'leftprimer_gc': '55.0', 'leftprimer_start': '22', 'name': '3520875', 'guidecount': 39, 'leftprimer_tm': '60.6053193565', 'product_length': 2522, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCCTTGTGCCGCTCAGTAAA', 'expandedpriming': 20}
{'rightprimer': 'CCTCAATTCACGCAGGAGGT', 'rightprimer_start': '3268', 'rightprimer_tm': '60.0360099994', 'leftprimer_length': '20', 'template_length': 3295, 'leftprimer_gc': '55.0', 'leftprimer_start': '5', 'name': '3999269', 'guidecount': 39, 'leftprimer_tm': '60.032216733', 'product_length': 3263, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGAGAGCTAGGCCTCTGCTT', 'expandedpriming': 0}
{'rightprimer': 'TGGGCAGACACCAGAATCAC', 'rightprimer_start': '3237', 'rightprimer_tm': '59.9632679558', 'leftprimer_length': '19', 'template_length': 3414, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '10', 'name': '6641451', 'guidecount': 39, 'leftprimer_tm': '59.999134083', 'product_length': 3227, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGGGAAAGCAAGCTGGGAT', 'expandedpriming': 20}
{'rightprimer': 'GGAGCACCATAGCTGAGGTAC', 'rightprimer_start': '3171', 'rightprimer_tm': '59.9306416698', 'leftprimer_length': '19', 'template_length': 3520, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '2', 'name': '9257947', 'guidecount': 39, 'leftprimer_tm': '59.7754075638', 'product_length': 3169, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'CTCCTGGGTATGCCACACC', 'expandedpriming': 0}
{'rightprimer': 'TTACTGTGTTGTGCTGCTTGC', 'rightprimer_start': '3034', 'rightprimer_tm': '59.9345989216', 'leftprimer_length': '22', 'template_length': 3084, 'leftprimer_gc': '54.5454545455', 'leftprimer_start': '73', 'name': '16458857', 'guidecount': 39, 'leftprimer_tm': '59.8977478281', 'product_length': 2961, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'CCCTTCTGCACTATGACTCTCC', 'expandedpriming': 50}
{'rightprimer': 'CACAGGGGGCCAAAGAGTAT', 'rightprimer_start': '2694', 'rightprimer_tm': '59.3741510867', 'leftprimer_length': '21', 'template_length': 2701, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '14', 'name': '32834244', 'guidecount': 39, 'leftprimer_tm': '59.5174072795', 'product_length': 2680, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACTCGGATAAGACTTGGGCAG', 'expandedpriming': 20}
{'rightprimer': 'GGGGCCCGGCACAGTCTT', 'rightprimer_start': '2079', 'rightprimer_tm': '64.747064635', 'leftprimer_length': '20', 'template_length': 2076, 'leftprimer_gc': '60.0', 'leftprimer_start': '268', 'name': '629157', 'guidecount': 38, 'leftprimer_tm': '63.4187279694', 'product_length': 1811, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'ATGCCTCAGTGCTTCACGGC', 'expandedpriming': 0}
{'rightprimer': 'CCACTGCTTTCTGGCGCTTA', 'rightprimer_start': '3256', 'rightprimer_tm': '60.9554282018', 'leftprimer_length': '20', 'template_length': 3304, 'leftprimer_gc': '55.0', 'leftprimer_start': '246', 'name': '4508696', 'guidecount': 38, 'leftprimer_tm': '60.8233559553', 'product_length': 3010, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGTGAAGAAGCACCCAAGGC', 'expandedpriming': 0}
{'rightprimer': 'GTAGGACCTTCTAGCTGTCCT', 'rightprimer_start': '2868', 'rightprimer_tm': '57.9788225634', 'leftprimer_length': '20', 'template_length': 2869, 'leftprimer_gc': '55.0', 'leftprimer_start': '6', 'name': '7111710', 'guidecount': 38, 'leftprimer_tm': '60.3216525037', 'product_length': 2862, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'CCCTAGCACCAAACGCTCTT', 'expandedpriming': 0}
{'rightprimer': 'GCCAAGAGCTTTGAACTAGG', 'rightprimer_start': '3151', 'rightprimer_tm': '56.4226577896', 'leftprimer_length': '22', 'template_length': 3143, 'leftprimer_gc': '40.9090909091', 'leftprimer_start': '20', 'name': '13227554', 'guidecount': 38, 'leftprimer_tm': '56.3442839552', 'product_length': 3131, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CCATGCGGGTTTATATCTGAAT', 'expandedpriming': 20}
{'rightprimer': 'GCCTGTCCAGTAATGCATAGC', 'rightprimer_start': '3051', 'rightprimer_tm': '59.123843778', 'leftprimer_length': '20', 'template_length': 3066, 'leftprimer_gc': '55.0', 'leftprimer_start': '2', 'name': '15564541', 'guidecount': 38, 'leftprimer_tm': '58.9884052488', 'product_length': 3049, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'AGCCCTCTAGGTTCTGAGGA', 'expandedpriming': 20}
{'rightprimer': 'TGTGCTGCTTTCTACTGACTGT', 'rightprimer_start': '2850', 'rightprimer_tm': '59.8969061168', 'leftprimer_length': '19', 'template_length': 2916, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '19', 'name': '17650036', 'guidecount': 38, 'leftprimer_tm': '59.7093606578', 'product_length': 2831, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'GATAGCACTAGAGGGGCGC', 'expandedpriming': 50}
{'rightprimer': 'TGCAGCGGTTCTTTTGAAACA', 'rightprimer_start': '5048', 'rightprimer_tm': '59.5255221989', 'leftprimer_length': '21', 'template_length': 5184, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '210', 'name': '18707125', 'guidecount': 38, 'leftprimer_tm': '59.5299016639', 'product_length': 4838, 'rightprimer_length': '21', 'rightprimer_gc': '42.8571428571', 'leftprimer': 'GCCTCTGCACACATGGTAAAG', 'expandedpriming': 0}
{'rightprimer': 'CCAAAGCTGAGAATCCCACC', 'rightprimer_start': '3062', 'rightprimer_tm': '58.5320801109', 'leftprimer_length': '25', 'template_length': 3080, 'leftprimer_gc': '40.0', 'leftprimer_start': '153', 'name': '19519332', 'guidecount': 38, 'leftprimer_tm': '57.6651560814', 'product_length': 2909, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAGTAGTCACACTAAGATATGGTCA', 'expandedpriming': 50}
{'rightprimer': 'CCATACCTCGTGGTTTCCCT', 'rightprimer_start': '2032', 'rightprimer_tm': '59.0903765276', 'leftprimer_length': '20', 'template_length': 2044, 'leftprimer_gc': '55.0', 'leftprimer_start': '201', 'name': '2517456', 'guidecount': 37, 'leftprimer_tm': '59.8921689425', 'product_length': 1831, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TAAGTGCCAGTGTGGGTGTC', 'expandedpriming': 20}
{'rightprimer': 'GCCTCCGGCCCACCCTAA', 'rightprimer_start': '4042', 'rightprimer_tm': '63.824484864', 'leftprimer_length': '20', 'template_length': 4040, 'leftprimer_gc': '50.0', 'leftprimer_start': '64', 'name': '4808269', 'guidecount': 37, 'leftprimer_tm': '60.3224559824', 'product_length': 3978, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'AGCGCTGGAATTGGCTACAA', 'expandedpriming': 0}
{'rightprimer': 'CGTGGGTCCATCCATAACGT', 'rightprimer_start': '3058', 'rightprimer_tm': '59.8228586037', 'leftprimer_length': '23', 'template_length': 3112, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '72', 'name': '6654244', 'guidecount': 37, 'leftprimer_tm': '59.5473732596', 'product_length': 2986, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AAGGAGTATCTGCAGGTTACAGG', 'expandedpriming': 50}
{'rightprimer': 'GAACTCCCAGATCCCCCCTA', 'rightprimer_start': '1289', 'rightprimer_tm': '59.7320399598', 'leftprimer_length': '20', 'template_length': 1272, 'leftprimer_gc': '60.0', 'leftprimer_start': '12', 'name': '10875048', 'guidecount': 37, 'leftprimer_tm': '59.8186498723', 'product_length': 1277, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCCTAGGGAATGTGGGAGTG', 'expandedpriming': 0}
{'rightprimer': 'GGGGGAGGCAGTTGCTAAAT', 'rightprimer_start': '4066', 'rightprimer_tm': '60.0331093374', 'leftprimer_length': '18', 'template_length': 4665, 'leftprimer_gc': '61.1111111111', 'leftprimer_start': '1', 'name': '33236792', 'guidecount': 37, 'leftprimer_tm': '59.9739863286', 'product_length': 4065, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCGGCGCCACATAATTG', 'expandedpriming': 0}
{'rightprimer': 'GCCATCTTTGAGCAGCATGC', 'rightprimer_start': '2032', 'rightprimer_tm': '60.5278165428', 'leftprimer_length': '20', 'template_length': 2030, 'leftprimer_gc': '55.0', 'leftprimer_start': '19', 'name': '2821256', 'guidecount': 36, 'leftprimer_tm': '60.4680287589', 'product_length': 2013, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCCTTGGGCACTTGAACAGT', 'expandedpriming': 20}
{'rightprimer': 'CACACAGGCCCGTACAGAC', 'rightprimer_start': '3412', 'rightprimer_tm': '60.3742516607', 'leftprimer_length': '20', 'template_length': 3447, 'leftprimer_gc': '55.0', 'leftprimer_start': '38', 'name': '9669231', 'guidecount': 36, 'leftprimer_tm': '59.8155485926', 'product_length': 3374, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'ATGGCATGGGAGGATGTGTC', 'expandedpriming': 50}
{'rightprimer': 'AATAAAACCGTGACCCCCCC', 'rightprimer_start': '2580', 'rightprimer_tm': '59.9599424387', 'leftprimer_length': '20', 'template_length': 2571, 'leftprimer_gc': '55.0', 'leftprimer_start': '202', 'name': '11608159', 'guidecount': 36, 'leftprimer_tm': '59.8188862673', 'product_length': 2378, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCCATAGCCTGTCACATCA', 'expandedpriming': 0}
{'rightprimer': 'GTGGGTAGCCTGGCTAATGT', 'rightprimer_start': '4255', 'rightprimer_tm': '59.4534017397', 'leftprimer_length': '23', 'template_length': 4257, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '23', 'name': '13220556', 'guidecount': 36, 'leftprimer_tm': '59.226880636', 'product_length': 4232, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACTCGGCATTAGGAGTTAGGAAA', 'expandedpriming': 20}
{'rightprimer': 'ACTGCTCCTAGCTGCCCT', 'rightprimer_start': '3224', 'rightprimer_tm': '59.9597222444', 'leftprimer_length': '20', 'template_length': 3228, 'leftprimer_gc': '60.0', 'leftprimer_start': '1', 'name': '26959427', 'guidecount': 36, 'leftprimer_tm': '59.7478029137', 'product_length': 3223, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'CTTATGGCCGGCTATACCCC', 'expandedpriming': 0}
{'rightprimer': 'GCTTGTGCACCCCCAAATAC', 'rightprimer_start': '2277', 'rightprimer_tm': '59.7536395582', 'leftprimer_length': '20', 'template_length': 2268, 'leftprimer_gc': '60.0', 'leftprimer_start': '30', 'name': '30101561', 'guidecount': 36, 'leftprimer_tm': '59.8223957703', 'product_length': 2247, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCCTGGAGAGATGTGAGCAC', 'expandedpriming': 20}
{'rightprimer': 'TCAGCCGCCGTGGAAATATT', 'rightprimer_start': '1868', 'rightprimer_tm': '60.1074104683', 'leftprimer_length': '23', 'template_length': 2169, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '23', 'name': '30355905', 'guidecount': 36, 'leftprimer_tm': '60.1184818093', 'product_length': 1845, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACCCAGCACAGACATGATTAGAG', 'expandedpriming': 0}
{'rightprimer': 'TGGGTTGTATCTAGGTGTACCC', 'rightprimer_start': '2358', 'rightprimer_tm': '58.6224265419', 'leftprimer_length': '20', 'template_length': 2359, 'leftprimer_gc': '55.0', 'leftprimer_start': '2', 'name': '185322', 'guidecount': 35, 'leftprimer_tm': '60.1791078772', 'product_length': 2356, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'GGATGTCTAGGAGCGCGTTT', 'expandedpriming': 20}
{'rightprimer': 'AAACCTGAGAAGCAGCCACT', 'rightprimer_start': '2387', 'rightprimer_tm': '59.5240937234', 'leftprimer_length': '21', 'template_length': 2450, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '5', 'name': '4171952', 'guidecount': 35, 'leftprimer_tm': '57.4448826034', 'product_length': 2382, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TTACTAGTCCCCTGTTCCACT', 'expandedpriming': 0}
{'rightprimer': 'GATGAGGCCCTCTCCCCTAT', 'rightprimer_start': '3809', 'rightprimer_tm': '59.8818560623', 'leftprimer_length': '22', 'template_length': 3931, 'leftprimer_gc': '50.0', 'leftprimer_start': '6', 'name': '4212945', 'guidecount': 35, 'leftprimer_tm': '59.8293933646', 'product_length': 3803, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCAGCCTAGTTTATCCCTGACA', 'expandedpriming': 0}
{'rightprimer': 'GGTTTCCGGCGGGATTCA', 'rightprimer_start': '2308', 'rightprimer_tm': '60.0457311816', 'leftprimer_length': '20', 'template_length': 2300, 'leftprimer_gc': '50.0', 'leftprimer_start': '103', 'name': '4452739', 'guidecount': 35, 'leftprimer_tm': '60.0326147522', 'product_length': 2205, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'TGCCGCCATTTTCCCCTTAT', 'expandedpriming': 0}
{'rightprimer': 'CCCCGGCCTACTCACGAG', 'rightprimer_start': '3795', 'rightprimer_tm': '61.5283698848', 'leftprimer_length': '20', 'template_length': 3804, 'leftprimer_gc': '55.0', 'leftprimer_start': '16', 'name': '6181841', 'guidecount': 35, 'leftprimer_tm': '60.8317112329', 'product_length': 3779, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'ATCTGGGTTTTGCACACCCC', 'expandedpriming': 20}
{'rightprimer': 'ACCCATTCCTCCATCTAGTCCA', 'rightprimer_start': '2309', 'rightprimer_tm': '60.0220347736', 'leftprimer_length': '20', 'template_length': 2322, 'leftprimer_gc': '55.0', 'leftprimer_start': '22', 'name': '6377472', 'guidecount': 35, 'leftprimer_tm': '59.7356406569', 'product_length': 2287, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'ATCTAGCCCTGTGAGCCTCT', 'expandedpriming': 20}
{'rightprimer': 'GCACACTGCCTACCACTCTT', 'rightprimer_start': '2809', 'rightprimer_tm': '59.9645424493', 'leftprimer_length': '22', 'template_length': 4308, 'leftprimer_gc': '50.0', 'leftprimer_start': '3', 'name': '7357475', 'guidecount': 35, 'leftprimer_tm': '59.699848273', 'product_length': 2806, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCCACTAGACACCAGCAAAGAG', 'expandedpriming': 0}
{'rightprimer': 'TTAAACTAAACTTGTACCCTTCGT', 'rightprimer_start': '4201', 'rightprimer_tm': '56.2591565903', 'leftprimer_length': '20', 'template_length': 4213, 'leftprimer_gc': '55.0', 'leftprimer_start': '205', 'name': '14973270', 'guidecount': 35, 'leftprimer_tm': '59.5300828439', 'product_length': 3996, 'rightprimer_length': '24', 'rightprimer_gc': '33.3333333333', 'leftprimer': 'ATTCACCTAGATGGGGCACG', 'expandedpriming': 20}
{'rightprimer': 'TCTCTCCAGGCTAGTCTGCA', 'rightprimer_start': '2834', 'rightprimer_tm': '59.6670282501', 'leftprimer_length': '20', 'template_length': 2830, 'leftprimer_gc': '60.0', 'leftprimer_start': '189', 'name': '15350548', 'guidecount': 35, 'leftprimer_tm': '59.9608196165', 'product_length': 2645, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCCAAACCTGGTACCTTCCC', 'expandedpriming': 20}
{'rightprimer': 'TCGGATCTAGGTGCAGTGGT', 'rightprimer_start': '2547', 'rightprimer_tm': '60.3243675663', 'leftprimer_length': '18', 'template_length': 2534, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '0', 'name': '15448458', 'guidecount': 35, 'leftprimer_tm': '60.6875657508', 'product_length': 2547, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGAGTAAGTGGGGCCGGG', 'expandedpriming': 0}
{'rightprimer': 'GCTGCCCGGCATACCAAG', 'rightprimer_start': '2666', 'rightprimer_tm': '61.2119626409', 'leftprimer_length': '22', 'template_length': 2659, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '2', 'name': '19248003', 'guidecount': 35, 'leftprimer_tm': '59.0803981312', 'product_length': 2664, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'TCTCCCCTGGAATTTAGCACTT', 'expandedpriming': 0}
{'rightprimer': 'CAAAGCCGGGTTCCTAGCC', 'rightprimer_start': '802', 'rightprimer_tm': '60.7492582642', 'leftprimer_length': '20', 'template_length': 787, 'leftprimer_gc': '55.0', 'leftprimer_start': '4', 'name': '25467961', 'guidecount': 35, 'leftprimer_tm': '60.322276626', 'product_length': 798, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'AACAGATGCTGCCTGGACTG', 'expandedpriming': 20}
{'rightprimer': 'AGTGGTTTAGGCGTAAATAGGGA', 'rightprimer_start': '3406', 'rightprimer_tm': '59.223582542', 'leftprimer_length': '20', 'template_length': 3455, 'leftprimer_gc': '50.0', 'leftprimer_start': '194', 'name': '27998084', 'guidecount': 35, 'leftprimer_tm': '58.9679860527', 'product_length': 3212, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'TTTTGAAGGTCAGTGCCTGC', 'expandedpriming': 50}
{'rightprimer': 'CACCCCGGGCTCCTTACT', 'rightprimer_start': '2749', 'rightprimer_tm': '60.6875657508', 'leftprimer_length': '21', 'template_length': 2755, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '3', 'name': '37213490', 'guidecount': 35, 'leftprimer_tm': '60.342274536', 'product_length': 2746, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'TGCCCCTGGATGGATTTTCAG', 'expandedpriming': 0}
{'rightprimer': 'TGGCAATAGGAGTGGCCAAG', 'rightprimer_start': '3411', 'rightprimer_tm': '60.0341860809', 'leftprimer_length': '20', 'template_length': 4243, 'leftprimer_gc': '60.0', 'leftprimer_start': '1', 'name': '37609299', 'guidecount': 35, 'leftprimer_tm': '60.4715905292', 'product_length': 3410, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCTCCATCTAGCCCTGCTCT', 'expandedpriming': 0}
{'rightprimer': 'ATGTTGTACCCGGGGGAAC', 'rightprimer_start': '1556', 'rightprimer_tm': '59.3129862531', 'leftprimer_length': '20', 'template_length': 1548, 'leftprimer_gc': '55.0', 'leftprimer_start': '48', 'name': '63893', 'guidecount': 34, 'leftprimer_tm': '59.8868101592', 'product_length': 1508, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'CCACTGGCCCCTTCTTTTCT', 'expandedpriming': 0}
{'rightprimer': 'AGGTGTGAGATGCCCAATGG', 'rightprimer_start': '2733', 'rightprimer_tm': '60.0337963284', 'leftprimer_length': '18', 'template_length': 2954, 'leftprimer_gc': '61.1111111111', 'leftprimer_start': '81', 'name': '530612', 'guidecount': 34, 'leftprimer_tm': '59.9659340369', 'product_length': 2652, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCGTCCCACACATGGCTT', 'expandedpriming': 0}
{'rightprimer': 'ACGAGAAGCCTTAGGGATGC', 'rightprimer_start': '2512', 'rightprimer_tm': '59.5338758623', 'leftprimer_length': '20', 'template_length': 2581, 'leftprimer_gc': '55.0', 'leftprimer_start': '11', 'name': '3516071', 'guidecount': 34, 'leftprimer_tm': '60.0360099994', 'product_length': 2501, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ATGGCTTCCGTTCCTCAGTG', 'expandedpriming': 0}
{'rightprimer': 'ATGGGATCTGATCACCCCCA', 'rightprimer_start': '4412', 'rightprimer_tm': '60.0287467454', 'leftprimer_length': '20', 'template_length': 4594, 'leftprimer_gc': '55.0', 'leftprimer_start': '106', 'name': '4462626', 'guidecount': 34, 'leftprimer_tm': '59.8914152912', 'product_length': 4306, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTGTCTCCTGCACCTTGGAA', 'expandedpriming': 0}
{'rightprimer': 'TGATGGGATCCTGGCCTT', 'rightprimer_start': '2743', 'rightprimer_tm': '57.4738975949', 'leftprimer_length': '20', 'template_length': 2735, 'leftprimer_gc': '60.0', 'leftprimer_start': '95', 'name': '6144424', 'guidecount': 34, 'leftprimer_tm': '59.8942215598', 'product_length': 2648, 'rightprimer_length': '18', 'rightprimer_gc': '55.5555555556', 'leftprimer': 'CTAGGGCTGCCAGTTAGAGC', 'expandedpriming': 20}
{'rightprimer': 'GACCAGTAGGGGGAACTCCT', 'rightprimer_start': '2671', 'rightprimer_tm': '59.9571185062', 'leftprimer_length': '20', 'template_length': 2723, 'leftprimer_gc': '55.0', 'leftprimer_start': '79', 'name': '7043469', 'guidecount': 34, 'leftprimer_tm': '59.8914152912', 'product_length': 2592, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GGGCACTGAGTGAAACTCCA', 'expandedpriming': 0}
{'rightprimer': 'GCCTTTAGTCTGGGTCCCAC', 'rightprimer_start': '1691', 'rightprimer_tm': '60.0353432429', 'leftprimer_length': '19', 'template_length': 1737, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '6', 'name': '10796247', 'guidecount': 34, 'leftprimer_tm': '59.8581189945', 'product_length': 1685, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CACCGGCTAGTACCTGCAG', 'expandedpriming': 0}
{'rightprimer': 'AGCTACTACTAGAGCAATAGGAAGT', 'rightprimer_start': '3866', 'rightprimer_tm': '58.3929812072', 'leftprimer_length': '20', 'template_length': 4126, 'leftprimer_gc': '50.0', 'leftprimer_start': '1309', 'name': '15264076', 'guidecount': 34, 'leftprimer_tm': '59.0018549342', 'product_length': 2557, 'rightprimer_length': '25', 'rightprimer_gc': '40.0', 'leftprimer': 'AAAGGCAGAGCTCCAGGAAT', 'expandedpriming': 20}
{'rightprimer': 'GGAGCCCACCCAGGTAAATT', 'rightprimer_start': '2854', 'rightprimer_tm': '59.6649812526', 'leftprimer_length': '21', 'template_length': 2838, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '5', 'name': '18085606', 'guidecount': 34, 'leftprimer_tm': '59.6384565367', 'product_length': 2849, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AAGTCCCTGCCAGGTAGTAGA', 'expandedpriming': 20}
{'rightprimer': 'GAAGCATAAGCGTCAGGTGT', 'rightprimer_start': '3711', 'rightprimer_tm': '58.2731466465', 'leftprimer_length': '21', 'template_length': 3774, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '66', 'name': '19700323', 'guidecount': 34, 'leftprimer_tm': '58.1835891037', 'product_length': 3645, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACGAATAACCTCAGTCTCCCA', 'expandedpriming': 50}
{'rightprimer': 'AGACCTGGTATAGAGTTTGCAACT', 'rightprimer_start': '2907', 'rightprimer_tm': '59.4674791838', 'leftprimer_length': '20', 'template_length': 2905, 'leftprimer_gc': '55.0', 'leftprimer_start': '254', 'name': '29471649', 'guidecount': 34, 'leftprimer_tm': '59.8868101592', 'product_length': 2653, 'rightprimer_length': '24', 'rightprimer_gc': '41.6666666667', 'leftprimer': 'CCCAGGAGTTTTCAGGGCTT', 'expandedpriming': 20}
{'rightprimer': 'CACACCTAGCAATGTGTGTGT', 'rightprimer_start': '1105', 'rightprimer_tm': '58.7788305298', 'leftprimer_length': '20', 'template_length': 1102, 'leftprimer_gc': '55.0', 'leftprimer_start': '4', 'name': '34903258', 'guidecount': 34, 'leftprimer_tm': '58.7606530193', 'product_length': 1101, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'GCCCAGTCTTTCACAAGGTC', 'expandedpriming': 20}
{'rightprimer': 'AGGATAATAACCCCCTCGCTCT', 'rightprimer_start': '2125', 'rightprimer_tm': '60.1581425139', 'leftprimer_length': '19', 'template_length': 2137, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '18', 'name': '37758526', 'guidecount': 34, 'leftprimer_tm': '60.4485404435', 'product_length': 2107, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'CAGTGCGATGGGAGTGGAC', 'expandedpriming': 20}
{'rightprimer': 'GCTGTGTTACTCTACTCTGACTAGG', 'rightprimer_start': '2015', 'rightprimer_tm': '59.9345259854', 'leftprimer_length': '20', 'template_length': 2016, 'leftprimer_gc': '55.0', 'leftprimer_start': '44', 'name': '2128407', 'guidecount': 33, 'leftprimer_tm': '60.0360099994', 'product_length': 1971, 'rightprimer_length': '25', 'rightprimer_gc': '48.0', 'leftprimer': 'CCGGAGCTTGATGAACTGGT', 'expandedpriming': 20}
{'rightprimer': 'TTTGCAGATATAAGTTGGATTCGG', 'rightprimer_start': '2245', 'rightprimer_tm': '57.3817133288', 'leftprimer_length': '20', 'template_length': 2326, 'leftprimer_gc': '45.0', 'leftprimer_start': '5', 'name': '3282206', 'guidecount': 33, 'leftprimer_tm': '57.3449104435', 'product_length': 2240, 'rightprimer_length': '24', 'rightprimer_gc': '37.5', 'leftprimer': 'TCACACCTGGAAAGCATTGA', 'expandedpriming': 0}
{'rightprimer': 'AGAGCAGCTCTACCTACACCA', 'rightprimer_start': '2079', 'rightprimer_tm': '59.9939573503', 'leftprimer_length': '20', 'template_length': 2094, 'leftprimer_gc': '60.0', 'leftprimer_start': '144', 'name': '6899919', 'guidecount': 33, 'leftprimer_tm': '60.3247376491', 'product_length': 1935, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'TACTCAGCACCCTGGAGAGG', 'expandedpriming': 20}
{'rightprimer': 'ATTAGTCCGGGGGCCAGAT', 'rightprimer_start': '1974', 'rightprimer_tm': '60.0737061259', 'leftprimer_length': '21', 'template_length': 1979, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '24', 'name': '8024894', 'guidecount': 33, 'leftprimer_tm': '59.7952958163', 'product_length': 1950, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'TGTACAATCCTCTCGCCTGTG', 'expandedpriming': 20}
{'rightprimer': 'GCCTTTGTGATGGCATAGCA', 'rightprimer_start': '1914', 'rightprimer_tm': '58.8945431708', 'leftprimer_length': '20', 'template_length': 1970, 'leftprimer_gc': '45.0', 'leftprimer_start': '37', 'name': '9710817', 'guidecount': 33, 'leftprimer_tm': '58.5774689213', 'product_length': 1877, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AAATGCAAGGGACAAAGGCA', 'expandedpriming': 50}
{'rightprimer': 'AGCAAGATGGCACTGATGTTT', 'rightprimer_start': '3903', 'rightprimer_tm': '58.4778150311', 'leftprimer_length': '20', 'template_length': 4119, 'leftprimer_gc': '50.0', 'leftprimer_start': '3', 'name': '9739234', 'guidecount': 33, 'leftprimer_tm': '58.5593819074', 'product_length': 3900, 'rightprimer_length': '21', 'rightprimer_gc': '42.8571428571', 'leftprimer': 'TGCCAACAGTCTAGGGGAAA', 'expandedpriming': 0}
{'rightprimer': 'CAGGTACGGGCTGAAGTCTA', 'rightprimer_start': '412', 'rightprimer_tm': '58.5288814267', 'leftprimer_length': '20', 'template_length': 407, 'leftprimer_gc': '55.0', 'leftprimer_start': '0', 'name': '10474017', 'guidecount': 33, 'leftprimer_tm': '58.8878871423', 'product_length': 412, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTGACGGGACTAGAATGGA', 'expandedpriming': 20}
{'rightprimer': 'CAGGAGAATCATGGCAACGC', 'rightprimer_start': '3454', 'rightprimer_tm': '59.6203305885', 'leftprimer_length': '22', 'template_length': 4341, 'leftprimer_gc': '40.9090909091', 'leftprimer_start': '67', 'name': '12685746', 'guidecount': 33, 'leftprimer_tm': '57.0895422239', 'product_length': 3387, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGGCTTGAACCTGTAGTACAAT', 'expandedpriming': 50}
{'rightprimer': 'GCAGCAAGTGACGCTTTACA', 'rightprimer_start': '1451', 'rightprimer_tm': '59.4136805798', 'leftprimer_length': '20', 'template_length': 1458, 'leftprimer_gc': '60.0', 'leftprimer_start': '2', 'name': '13242653', 'guidecount': 33, 'leftprimer_tm': '59.7466731477', 'product_length': 1449, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGGGGGCTAGACTGAAAGTG', 'expandedpriming': 20}
{'rightprimer': 'AGTAGCCAACACAGCTCACT', 'rightprimer_start': '3447', 'rightprimer_tm': '58.948188082', 'leftprimer_length': '24', 'template_length': 3478, 'leftprimer_gc': '41.6666666667', 'leftprimer_start': '0', 'name': '15405685', 'guidecount': 33, 'leftprimer_tm': '58.0927934485', 'product_length': 3447, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GTTATCTGCTAGTGTGGAATCTGA', 'expandedpriming': 0}
{'rightprimer': 'TCAGAAACACGTGCATTCTTGTT', 'rightprimer_start': '4888', 'rightprimer_tm': '59.6231414423', 'leftprimer_length': '21', 'template_length': 4888, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '195', 'name': '17688361', 'guidecount': 33, 'leftprimer_tm': '59.7235785279', 'product_length': 4693, 'rightprimer_length': '23', 'rightprimer_gc': '39.1304347826', 'leftprimer': 'AGCGGAGACATTAGCAGTTGT', 'expandedpriming': 20}
{'rightprimer': 'GTTTTAACCCCAGGCTGCTAAA', 'rightprimer_start': '2157', 'rightprimer_tm': '59.1052352201', 'leftprimer_length': '18', 'template_length': 2149, 'leftprimer_gc': '72.2222222222', 'leftprimer_start': '1', 'name': '18101586', 'guidecount': 33, 'leftprimer_tm': '64.0878971375', 'product_length': 2156, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'CCCCGGCGGACTGGACTT', 'expandedpriming': 0}
{'rightprimer': 'CCGGGGGAACTCATGGTG', 'rightprimer_start': '1010', 'rightprimer_tm': '59.7278539951', 'leftprimer_length': '20', 'template_length': 1012, 'leftprimer_gc': '55.0', 'leftprimer_start': '145', 'name': '33207283', 'guidecount': 33, 'leftprimer_tm': '59.7506019163', 'product_length': 865, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'TGACTAGAAGCTTTCGGCCC', 'expandedpriming': 20}
{'rightprimer': 'ACGGTGCTAGCTCTCTCCAT', 'rightprimer_start': '2786', 'rightprimer_tm': '60.3964168222', 'leftprimer_length': '20', 'template_length': 2782, 'leftprimer_gc': '55.0', 'leftprimer_start': '626', 'name': '37245637', 'guidecount': 33, 'leftprimer_tm': '60.0341860809', 'product_length': 2160, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TAATGACCAGCCAAGCCCTG', 'expandedpriming': 0}
{'rightprimer': 'CCCAACCAAAGGGCCAGATT', 'rightprimer_start': '1108', 'rightprimer_tm': '60.5450707466', 'leftprimer_length': '18', 'template_length': 1164, 'leftprimer_gc': '72.2222222222', 'leftprimer_start': '9', 'name': '949157', 'guidecount': 32, 'leftprimer_tm': '64.8580771309', 'product_length': 1099, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCCGGGGCAGGAATGGCA', 'expandedpriming': 0}
{'rightprimer': 'TGCTGGCTGTGTTAGACTGT', 'rightprimer_start': '1928', 'rightprimer_tm': '59.2412660313', 'leftprimer_length': '20', 'template_length': 1918, 'leftprimer_gc': '55.0', 'leftprimer_start': '9', 'name': '7327175', 'guidecount': 32, 'leftprimer_tm': '59.3825872816', 'product_length': 1919, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CCTGAGACTGGTCCAATGCT', 'expandedpriming': 20}
{'rightprimer': 'GGAAAAGCTTCTGTTGGCCG', 'rightprimer_start': '2565', 'rightprimer_tm': '60.0391648998', 'leftprimer_length': '20', 'template_length': 2686, 'leftprimer_gc': '60.0', 'leftprimer_start': '214', 'name': '13565128', 'guidecount': 32, 'leftprimer_tm': '60.0359151165', 'product_length': 2351, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTTGGCTCCCTCTTCTGTCC', 'expandedpriming': 0}
{'rightprimer': 'GGAGGGTATGGGGGTATTGG', 'rightprimer_start': '3222', 'rightprimer_tm': '58.926902167', 'leftprimer_length': '22', 'template_length': 3224, 'leftprimer_gc': '50.0', 'leftprimer_start': '9', 'name': '20141492', 'guidecount': 32, 'leftprimer_tm': '58.9152143756', 'product_length': 3213, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCTGGACTATGAATTATGGGGC', 'expandedpriming': 20}
{'rightprimer': 'GACTTCCTGGTAATGCCCCC', 'rightprimer_start': '2030', 'rightprimer_tm': '60.1066692046', 'leftprimer_length': '22', 'template_length': 2035, 'leftprimer_gc': '50.0', 'leftprimer_start': '23', 'name': '25527705', 'guidecount': 32, 'leftprimer_tm': '59.5693057175', 'product_length': 2007, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GGCAACCCTATTCAATGAACCC', 'expandedpriming': 20}
{'rightprimer': 'CACAAAGGCCCGGTCCAG', 'rightprimer_start': '3700', 'rightprimer_tm': '60.6729958012', 'leftprimer_length': '20', 'template_length': 3693, 'leftprimer_gc': '55.0', 'leftprimer_start': '74', 'name': '28148710', 'guidecount': 32, 'leftprimer_tm': '60.2510766903', 'product_length': 3626, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'AACTTGCTCAAGCCCAGAGG', 'expandedpriming': 0}
{'rightprimer': 'GGCGTCTCTCTCCCCAGATA', 'rightprimer_start': '4281', 'rightprimer_tm': '60.1789867582', 'leftprimer_length': '20', 'template_length': 4339, 'leftprimer_gc': '55.0', 'leftprimer_start': '19', 'name': '1171981', 'guidecount': 31, 'leftprimer_tm': '60.6799600556', 'product_length': 4262, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCACCCACCGCTCCTTTTAT', 'expandedpriming': 50}
{'rightprimer': 'AACCCTTGCCCAGAAACTGT', 'rightprimer_start': '1527', 'rightprimer_tm': '59.7383036977', 'leftprimer_length': '20', 'template_length': 1602, 'leftprimer_gc': '45.0', 'leftprimer_start': '56', 'name': '2406680', 'guidecount': 31, 'leftprimer_tm': '59.048442211', 'product_length': 1471, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AGCAAGAACGCGATTGGTTT', 'expandedpriming': 50}
{'rightprimer': 'CATGGAAGTTTGCGCCAAGT', 'rightprimer_start': '1636', 'rightprimer_tm': '59.685065357', 'leftprimer_length': '20', 'template_length': 1789, 'leftprimer_gc': '60.0', 'leftprimer_start': '0', 'name': '2804573', 'guidecount': 31, 'leftprimer_tm': '59.4461811443', 'product_length': 1636, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TCCTCCCTCTAGCTGTCTCC', 'expandedpriming': 0}
{'rightprimer': 'CCCCTGCCGGCAGTGTTG', 'rightprimer_start': '1368', 'rightprimer_tm': '63.704635961', 'leftprimer_length': '20', 'template_length': 1364, 'leftprimer_gc': '55.0', 'leftprimer_start': '91', 'name': '3112460', 'guidecount': 31, 'leftprimer_tm': '60.4629374006', 'product_length': 1277, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'CGGTGGAACTGTGACAACCA', 'expandedpriming': 0}
{'rightprimer': 'ACCAAAGGCTAACCGTGGAG', 'rightprimer_start': '1967', 'rightprimer_tm': '59.9636944336', 'leftprimer_length': '21', 'template_length': 2223, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '12', 'name': '3409736', 'guidecount': 31, 'leftprimer_tm': '59.9930020551', 'product_length': 1955, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGGCCATATTGAAACAGCCCT', 'expandedpriming': 20}
{'rightprimer': 'AGCAGAGCGGCATTCTACAG', 'rightprimer_start': '1487', 'rightprimer_tm': '60.1790890618', 'leftprimer_length': '21', 'template_length': 1484, 'leftprimer_gc': '57.1428571429', 'leftprimer_start': '4', 'name': '3519390', 'guidecount': 31, 'leftprimer_tm': '60.0663582513', 'product_length': 1483, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTTCCAGGTAGGTACTGTGCC', 'expandedpriming': 0}
{'rightprimer': 'CCCCCCCAGGCATTCCTAT', 'rightprimer_start': '3239', 'rightprimer_tm': '60.7800657657', 'leftprimer_length': '18', 'template_length': 3235, 'leftprimer_gc': '61.1111111111', 'leftprimer_start': '0', 'name': '7076647', 'guidecount': 31, 'leftprimer_tm': '61.310307029', 'product_length': 3239, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'AAACATTGCGGTGGGCCC', 'expandedpriming': 0}
{'rightprimer': 'GCCACTCATTTCCCTGGTCT', 'rightprimer_start': '3131', 'rightprimer_tm': '59.67040422', 'leftprimer_length': '21', 'template_length': 3306, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '22', 'name': '7097507', 'guidecount': 31, 'leftprimer_tm': '59.5052785369', 'product_length': 3109, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTTCTTTGTCCTGCAGGGTCT', 'expandedpriming': 20}
{'rightprimer': 'CAGTAACTCCCCTCGGCTTC', 'rightprimer_start': '2247', 'rightprimer_tm': '59.8240105107', 'leftprimer_length': '25', 'template_length': 2453, 'leftprimer_gc': '40.0', 'leftprimer_start': '1', 'name': '11161470', 'guidecount': 31, 'leftprimer_tm': '59.7501478608', 'product_length': 2246, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'AACCAACTCTAGTATTTACCAGCCA', 'expandedpriming': 0}
{'rightprimer': 'CATGTGACAGCTAGGGGATG', 'rightprimer_start': '2885', 'rightprimer_tm': '57.748591028', 'leftprimer_length': '20', 'template_length': 2877, 'leftprimer_gc': '50.0', 'leftprimer_start': '764', 'name': '14942140', 'guidecount': 31, 'leftprimer_tm': '58.9679860527', 'product_length': 2121, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTCCAAGAAACAGCACAGCC', 'expandedpriming': 0}
{'rightprimer': 'GCAATGCTTGTGCCCTTTCT', 'rightprimer_start': '4118', 'rightprimer_tm': '59.6805947339', 'leftprimer_length': '21', 'template_length': 4120, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '21', 'name': '15617659', 'guidecount': 31, 'leftprimer_tm': '59.854880852', 'product_length': 4097, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGGAAAGTGTTCTCTTGGGGT', 'expandedpriming': 20}
{'rightprimer': 'AGATCAGAACAAGATGAGACCATT', 'rightprimer_start': '2253', 'rightprimer_tm': '57.689235747', 'leftprimer_length': '20', 'template_length': 2257, 'leftprimer_gc': '55.0', 'leftprimer_start': '471', 'name': '25443874', 'guidecount': 31, 'leftprimer_tm': '59.3115129283', 'product_length': 1782, 'rightprimer_length': '24', 'rightprimer_gc': '37.5', 'leftprimer': 'CCGGTTCCTGTGTTTCCCTA', 'expandedpriming': 20}
{'rightprimer': 'ACACAAATACCAGGACAAGAGA', 'rightprimer_start': '2640', 'rightprimer_tm': '57.0253553811', 'leftprimer_length': '20', 'template_length': 2632, 'leftprimer_gc': '50.0', 'leftprimer_start': '158', 'name': '25729946', 'guidecount': 31, 'leftprimer_tm': '58.4222638363', 'product_length': 2482, 'rightprimer_length': '22', 'rightprimer_gc': '40.9090909091', 'leftprimer': 'AGCAGCTGGAGAATGGTACT', 'expandedpriming': 0}
{'rightprimer': 'TTTAACCCCCTAGAGGTTTCTTT', 'rightprimer_start': '3298', 'rightprimer_tm': '57.0643318096', 'leftprimer_length': '19', 'template_length': 3291, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '4', 'name': '25882267', 'guidecount': 31, 'leftprimer_tm': '59.8537374332', 'product_length': 3294, 'rightprimer_length': '23', 'rightprimer_gc': '39.1304347826', 'leftprimer': 'CCCCGGCGCTCTTTCTTAT', 'expandedpriming': 0}
{'rightprimer': 'AGCAGCCACACACATGATCA', 'rightprimer_start': '1577', 'rightprimer_tm': '59.9628398186', 'leftprimer_length': '22', 'template_length': 1762, 'leftprimer_gc': '50.0', 'leftprimer_start': '2', 'name': '26766673', 'guidecount': 31, 'leftprimer_tm': '59.5130183205', 'product_length': 1575, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GTTGCTAGGTAGAGTGACGTCA', 'expandedpriming': 0}
{'rightprimer': 'AAAACTAGTCAGGCCCCACC', 'rightprimer_start': '2338', 'rightprimer_tm': '59.5955740658', 'leftprimer_length': '18', 'template_length': 2360, 'leftprimer_gc': '72.2222222222', 'leftprimer_start': '2', 'name': '29870028', 'guidecount': 31, 'leftprimer_tm': '63.3971331212', 'product_length': 2336, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCACTTCCGGGTGCTGCC', 'expandedpriming': 20}
{'rightprimer': 'AATCATCTTGAGGAGGCCCC', 'rightprimer_start': '2219', 'rightprimer_tm': '59.1505830269', 'leftprimer_length': '18', 'template_length': 2268, 'leftprimer_gc': '61.1111111111', 'leftprimer_start': '0', 'name': '32288903', 'guidecount': 31, 'leftprimer_tm': '56.7078383018', 'product_length': 2219, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCCTGTGCCTAGTGATGG', 'expandedpriming': 0}
{'rightprimer': 'AGTGGTGTGGCCTTTGCTC', 'rightprimer_start': '1174', 'rightprimer_tm': '60.5292436765', 'leftprimer_length': '19', 'template_length': 1216, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '6', 'name': '76168', 'guidecount': 30, 'leftprimer_tm': '60.6124422993', 'product_length': 1168, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'GGGCTACAGTCAGGGACCA', 'expandedpriming': 50}
{'rightprimer': 'GATAGCCCTAGGGAGAGGCA', 'rightprimer_start': '1279', 'rightprimer_tm': '59.8846505619', 'leftprimer_length': '22', 'template_length': 1416, 'leftprimer_gc': '50.0', 'leftprimer_start': '122', 'name': '606010', 'guidecount': 30, 'leftprimer_tm': '60.0243685006', 'product_length': 1157, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TGTATCTAGAGGTCTGGGCACA', 'expandedpriming': 20}
{'rightprimer': 'TCTGGTTTGTGTGTCACGGA', 'rightprimer_start': '2034', 'rightprimer_tm': '59.4623472489', 'leftprimer_length': '20', 'template_length': 2118, 'leftprimer_gc': '50.0', 'leftprimer_start': '215', 'name': '2086941', 'guidecount': 30, 'leftprimer_tm': '59.7579825863', 'product_length': 1819, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AATGTTTTCTCCAGCGCTGC', 'expandedpriming': 0}
{'rightprimer': 'GGAGTCCTGGGAGATGGAG', 'rightprimer_start': '2334', 'rightprimer_tm': '58.1667560581', 'leftprimer_length': '20', 'template_length': 2330, 'leftprimer_gc': '50.0', 'leftprimer_start': '1', 'name': '2300498', 'guidecount': 30, 'leftprimer_tm': '57.3252044922', 'product_length': 2333, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'AACCGGATGCTTCTCTACAC', 'expandedpriming': 0}
{'rightprimer': 'CACTTATGTTGCCACGCTGG', 'rightprimer_start': '1545', 'rightprimer_tm': '59.83121188', 'leftprimer_length': '20', 'template_length': 1771, 'leftprimer_gc': '55.0', 'leftprimer_start': '1', 'name': '2309948', 'guidecount': 30, 'leftprimer_tm': '59.5466101613', 'product_length': 1544, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTTCAGCCCGGAATTTCAG', 'expandedpriming': 0}
{'rightprimer': 'GTCCCCTTTAGATCCCCCCT', 'rightprimer_start': '3253', 'rightprimer_tm': '60.0289552677', 'leftprimer_length': '19', 'template_length': 3619, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '1', 'name': '2765441', 'guidecount': 30, 'leftprimer_tm': '59.8550642485', 'product_length': 3252, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CTCCCTTCAGATGGGCGAC', 'expandedpriming': 0}
{'rightprimer': 'TACTGCTCAGCCTACCACCT', 'rightprimer_start': '2905', 'rightprimer_tm': '59.9587620532', 'leftprimer_length': '25', 'template_length': 2921, 'leftprimer_gc': '36.0', 'leftprimer_start': '2', 'name': '3259221', 'guidecount': 30, 'leftprimer_tm': '58.0986996567', 'product_length': 2903, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGAAAACTAGTTTCCTGGAACTCTT', 'expandedpriming': 20}
{'rightprimer': 'CCAACCTGGCCGCCATAG', 'rightprimer_start': '3680', 'rightprimer_tm': '60.5169302646', 'leftprimer_length': '20', 'template_length': 3677, 'leftprimer_gc': '60.0', 'leftprimer_start': '13', 'name': '3482500', 'guidecount': 30, 'leftprimer_tm': '60.9881450966', 'product_length': 3667, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'CCAGGGTGGGATGGCTCTTA', 'expandedpriming': 0}
{'rightprimer': 'CCATGCCGGGATCCTTTCA', 'rightprimer_start': '1172', 'rightprimer_tm': '59.7728977681', 'leftprimer_length': '20', 'template_length': 1168, 'leftprimer_gc': '55.0', 'leftprimer_start': '72', 'name': '4260837', 'guidecount': 30, 'leftprimer_tm': '59.9626967358', 'product_length': 1100, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'CTGCCACTGTCATGTCCCTT', 'expandedpriming': 0}
{'rightprimer': 'TACCTTGCGAACACCCCATC', 'rightprimer_start': '1666', 'rightprimer_tm': '60.0358201114', 'leftprimer_length': '20', 'template_length': 1890, 'leftprimer_gc': '50.0', 'leftprimer_start': '117', 'name': '4483346', 'guidecount': 30, 'leftprimer_tm': '60.1062679146', 'product_length': 1549, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGCAAGGCAAGAGGGAACAA', 'expandedpriming': 0}
{'rightprimer': 'GCTTCAGTTATACCACTAGACAGCT', 'rightprimer_start': '3283', 'rightprimer_tm': '60.1634988129', 'leftprimer_length': '20', 'template_length': 3277, 'leftprimer_gc': '55.0', 'leftprimer_start': '74', 'name': '4895826', 'guidecount': 30, 'leftprimer_tm': '60.1072142032', 'product_length': 3209, 'rightprimer_length': '25', 'rightprimer_gc': '44.0', 'leftprimer': 'CCAGCCCACCATGTAACGAT', 'expandedpriming': 20}
{'rightprimer': 'TCCGGTTCTTGTTGTGTTCT', 'rightprimer_start': '3417', 'rightprimer_tm': '57.297408052', 'leftprimer_length': '22', 'template_length': 3487, 'leftprimer_gc': '40.9090909091', 'leftprimer_start': '58', 'name': '5954309', 'guidecount': 30, 'leftprimer_tm': '57.2931581288', 'product_length': 3359, 'rightprimer_length': '20', 'rightprimer_gc': '45.0', 'leftprimer': 'ACAGGGTCTGTTCATACAAGTT', 'expandedpriming': 50}
{'rightprimer': 'CCTGGTCAAGAGTGGGGTTAC', 'rightprimer_start': '2755', 'rightprimer_tm': '59.9967665524', 'leftprimer_length': '20', 'template_length': 2756, 'leftprimer_gc': '60.0', 'leftprimer_start': '30', 'name': '6147176', 'guidecount': 30, 'leftprimer_tm': '60.0356297333', 'product_length': 2725, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'CCAGAGAGCCCTCCACAAAG', 'expandedpriming': 20}
{'rightprimer': 'CTCCTCACCCTCCTTGCTTG', 'rightprimer_start': '2888', 'rightprimer_tm': '60.0356297333', 'leftprimer_length': '20', 'template_length': 2994, 'leftprimer_gc': '55.0', 'leftprimer_start': '76', 'name': '6712294', 'guidecount': 30, 'leftprimer_tm': '59.964683149', 'product_length': 2812, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCTGTGTGTCAGGATGGTGA', 'expandedpriming': 50}
{'rightprimer': 'TGCTTGGCACTCATGTACCT', 'rightprimer_start': '1921', 'rightprimer_tm': '59.3075242596', 'leftprimer_length': '21', 'template_length': 1927, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '7', 'name': '7212785', 'guidecount': 30, 'leftprimer_tm': '59.1600057842', 'product_length': 1914, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGAATCTGATGTGTGGGGAGA', 'expandedpriming': 20}
{'rightprimer': 'GGCCTTTTCCTAGTACTCCC', 'rightprimer_start': '2057', 'rightprimer_tm': '56.9954688925', 'leftprimer_length': '21', 'template_length': 2059, 'leftprimer_gc': '42.8571428571', 'leftprimer_start': '4', 'name': '8438721', 'guidecount': 30, 'leftprimer_tm': '56.2665642779', 'product_length': 2053, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AACCCTGGAGTCAACTATTGA', 'expandedpriming': 0}
{'rightprimer': 'CTCCCTCTGGTCCTGTGAGA', 'rightprimer_start': '2141', 'rightprimer_tm': '59.9596485071', 'leftprimer_length': '20', 'template_length': 2181, 'leftprimer_gc': '55.0', 'leftprimer_start': '27', 'name': '10933071', 'guidecount': 30, 'leftprimer_tm': '59.9697647791', 'product_length': 2114, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GTCGGGCACAACAGTTTTCC', 'expandedpriming': 0}
{'rightprimer': 'CTGCAGAGACCCACATGTGA', 'rightprimer_start': '2949', 'rightprimer_tm': '59.6773519983', 'leftprimer_length': '20', 'template_length': 2982, 'leftprimer_gc': '55.0', 'leftprimer_start': '13', 'name': '11642867', 'guidecount': 30, 'leftprimer_tm': '59.6031890731', 'product_length': 2936, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACAACCAGGTAGCACTCACC', 'expandedpriming': 20}
{'rightprimer': 'CAACTAGCTGACCCCCTTTGT', 'rightprimer_start': '2802', 'rightprimer_tm': '59.9260765809', 'leftprimer_length': '20', 'template_length': 2917, 'leftprimer_gc': '50.0', 'leftprimer_start': '160', 'name': '14234598', 'guidecount': 30, 'leftprimer_tm': '59.8858324082', 'product_length': 2642, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'TAATTGCCACAAGGGGCAGT', 'expandedpriming': 20}
{'rightprimer': 'AGTGAAGAAACTAGCCACCAGT', 'rightprimer_start': '3931', 'rightprimer_tm': '59.2938096801', 'leftprimer_length': '23', 'template_length': 3932, 'leftprimer_gc': '52.1739130435', 'leftprimer_start': '1', 'name': '16652947', 'guidecount': 30, 'leftprimer_tm': '59.5499035905', 'product_length': 3930, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'CCCTTACCTAGTGTACCAAGGTC', 'expandedpriming': 0}
{'rightprimer': 'GGGTGAGATTTGTGATGCTTGG', 'rightprimer_start': '4708', 'rightprimer_tm': '59.834969528', 'leftprimer_length': '20', 'template_length': 4705, 'leftprimer_gc': '60.0', 'leftprimer_start': '8', 'name': '20162826', 'guidecount': 30, 'leftprimer_tm': '59.8917926039', 'product_length': 4700, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'CAGGAGGCTGCTTATCAGGG', 'expandedpriming': 20}
{'rightprimer': 'GTGTCCTGGGATTGGAAGGG', 'rightprimer_start': '1807', 'rightprimer_tm': '60.0340888334', 'leftprimer_length': '24', 'template_length': 1821, 'leftprimer_gc': '45.8333333333', 'leftprimer_start': '1', 'name': '21121761', 'guidecount': 30, 'leftprimer_tm': '59.9579375541', 'product_length': 1806, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TTGGATCCAGGTTAGCATATCTGG', 'expandedpriming': 20}
{'rightprimer': 'CCCCATTTCCCCCTTGTACC', 'rightprimer_start': '6326', 'rightprimer_tm': '60.0325154441', 'leftprimer_length': '22', 'template_length': 6593, 'leftprimer_gc': '50.0', 'leftprimer_start': '221', 'name': '21452687', 'guidecount': 30, 'leftprimer_tm': '60.0268216746', 'product_length': 6105, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCAATGAGACTCCCTCTTTCCA', 'expandedpriming': 0}
{'rightprimer': 'AAGCTCACCTGGTGCAAGAG', 'rightprimer_start': '921', 'rightprimer_tm': '60.2506401115', 'leftprimer_length': '19', 'template_length': 946, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '3', 'name': '28393245', 'guidecount': 30, 'leftprimer_tm': '60.3734966123', 'product_length': 918, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAGTGCCGGTGAGTCTGAC', 'expandedpriming': 20}
{'rightprimer': 'CAGACGCCCCCCGGTCTC', 'rightprimer_start': '2317', 'rightprimer_tm': '64.4928310611', 'leftprimer_length': '20', 'template_length': 2309, 'leftprimer_gc': '65.0', 'leftprimer_start': '101', 'name': '29120317', 'guidecount': 30, 'leftprimer_tm': '63.7191103317', 'product_length': 2216, 'rightprimer_length': '18', 'rightprimer_gc': '77.7777777778', 'leftprimer': 'CCATCACAGCACTGGGTGGC', 'expandedpriming': 0}
{'rightprimer': 'GTGGCTCTAGCGAGAACGTC', 'rightprimer_start': '1072', 'rightprimer_tm': '60.5237571467', 'leftprimer_length': '21', 'template_length': 1055, 'leftprimer_gc': '57.1428571429', 'leftprimer_start': '11', 'name': '29710172', 'guidecount': 30, 'leftprimer_tm': '60.4146846907', 'product_length': 1061, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GGTCACTAGAATAGGGGGGCT', 'expandedpriming': 0}
{'rightprimer': 'GCATACCCTAGCAGAAGGTAC', 'rightprimer_start': '2239', 'rightprimer_tm': '57.6116545241', 'leftprimer_length': '20', 'template_length': 2231, 'leftprimer_gc': '45.0', 'leftprimer_start': '3', 'name': '29783190', 'guidecount': 30, 'leftprimer_tm': '56.7243531351', 'product_length': 2236, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'ACAATCTAGTGCCACCCTTT', 'expandedpriming': 0}
{'rightprimer': 'CACTCACCCAGGGTAAGGAAC', 'rightprimer_start': '3515', 'rightprimer_tm': '59.9967665524', 'leftprimer_length': '20', 'template_length': 3509, 'leftprimer_gc': '60.0', 'leftprimer_start': '4', 'name': '30084092', 'guidecount': 30, 'leftprimer_tm': '59.5363801648', 'product_length': 3511, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'CTTCCAGGAGGCTCTACAGC', 'expandedpriming': 0}
{'rightprimer': 'GGGGGGGAAAGTTCTAGCAG', 'rightprimer_start': '1114', 'rightprimer_tm': '59.7438229227', 'leftprimer_length': '21', 'template_length': 1118, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '10', 'name': '30690211', 'guidecount': 30, 'leftprimer_tm': '59.5090896442', 'product_length': 1104, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GGGAGGGCTACATTAACAGCT', 'expandedpriming': 20}
{'rightprimer': 'ACAGCGCTAGCCATTTCAGA', 'rightprimer_start': '704', 'rightprimer_tm': '59.7497660004', 'leftprimer_length': '19', 'template_length': 698, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '12', 'name': '34034464', 'guidecount': 30, 'leftprimer_tm': '59.706501831', 'product_length': 692, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ATCCGGCAGATATCCGTGC', 'expandedpriming': 20}
{'rightprimer': 'CCACATGATCCCGGCACTC', 'rightprimer_start': '2027', 'rightprimer_tm': '60.5246206524', 'leftprimer_length': '20', 'template_length': 2016, 'leftprimer_gc': '55.0', 'leftprimer_start': '230', 'name': '37206811', 'guidecount': 30, 'leftprimer_tm': '60.2499107018', 'product_length': 1797, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'AGTGCCTTTGTCGTCACTCC', 'expandedpriming': 0}
{'rightprimer': 'ACAAGAAGCTTGGCCCATCA', 'rightprimer_start': '2150', 'rightprimer_tm': '59.8870049386', 'leftprimer_length': '20', 'template_length': 2161, 'leftprimer_gc': '55.0', 'leftprimer_start': '14', 'name': '37233991', 'guidecount': 30, 'leftprimer_tm': '59.7486464091', 'product_length': 2136, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GCAAGATGCCAGAGAGGTGA', 'expandedpriming': 20}
{'rightprimer': 'TAGCCATGGGAGCCAGTACT', 'rightprimer_start': '1045', 'rightprimer_tm': '60.0311112616', 'leftprimer_length': '20', 'template_length': 1041, 'leftprimer_gc': '50.0', 'leftprimer_start': '9', 'name': '37685488', 'guidecount': 30, 'leftprimer_tm': '60.1790591538', 'product_length': 1036, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCAGACAAGCAAAGTGCCGA', 'expandedpriming': 20}
{'rightprimer': 'CTGCTCAACATTAGATGACAGGA', 'rightprimer_start': '2655', 'rightprimer_tm': '58.2369270333', 'leftprimer_length': '20', 'template_length': 2699, 'leftprimer_gc': '45.0', 'leftprimer_start': '232', 'name': '208527', 'guidecount': 29, 'leftprimer_tm': '58.2065861192', 'product_length': 2423, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'AGGCCTTGTTTGAAAAGCCT', 'expandedpriming': 50}
{'rightprimer': 'CCATTCGCTCTGCTGTGTGG', 'rightprimer_start': '1780', 'rightprimer_tm': '61.6420368166', 'leftprimer_length': '20', 'template_length': 1790, 'leftprimer_gc': '60.0', 'leftprimer_start': '85', 'name': '782458', 'guidecount': 29, 'leftprimer_tm': '61.5861543949', 'product_length': 1695, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCGGCACAACTGATGGGAAC', 'expandedpriming': 20}
{'rightprimer': 'GGGCATCAATAGGAATCTAATATCT', 'rightprimer_start': '1250', 'rightprimer_tm': '56.0896547539', 'leftprimer_length': '20', 'template_length': 1383, 'leftprimer_gc': '45.0', 'leftprimer_start': '66', 'name': '815190', 'guidecount': 29, 'leftprimer_tm': '56.096088531', 'product_length': 1184, 'rightprimer_length': '25', 'rightprimer_gc': '36.0', 'leftprimer': 'CGAAGTCTGATTTGCTGTGA', 'expandedpriming': 50}
{'rightprimer': 'GCCCCTACCAGACACTTATTCC', 'rightprimer_start': '3221', 'rightprimer_tm': '60.1587983213', 'leftprimer_length': '18', 'template_length': 3230, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '19', 'name': '3154022', 'guidecount': 29, 'leftprimer_tm': '60.5883144525', 'product_length': 3202, 'rightprimer_length': '22', 'rightprimer_gc': '54.5454545455', 'leftprimer': 'CTCAGAGATGCCGCTGCC', 'expandedpriming': 20}
{'rightprimer': 'TGGGTTTCCTGGTTGTGATGT', 'rightprimer_start': '1502', 'rightprimer_tm': '59.7833216542', 'leftprimer_length': '20', 'template_length': 1491, 'leftprimer_gc': '45.0', 'leftprimer_start': '47', 'name': '3939647', 'guidecount': 29, 'leftprimer_tm': '59.8953290178', 'product_length': 1455, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'AAGCAACATGGCGCAAAGTT', 'expandedpriming': 0}
{'rightprimer': 'ACCCCCTGGCACCAACAT', 'rightprimer_start': '2686', 'rightprimer_tm': '61.1926302568', 'leftprimer_length': '20', 'template_length': 2683, 'leftprimer_gc': '60.0', 'leftprimer_start': '214', 'name': '7363550', 'guidecount': 29, 'leftprimer_tm': '60.9797086567', 'product_length': 2472, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'GGGGGAAGCGTAGGGTTAGT', 'expandedpriming': 0}
{'rightprimer': 'TGAGCTCCTGGATGGATGGA', 'rightprimer_start': '1589', 'rightprimer_tm': '60.0308069568', 'leftprimer_length': '25', 'template_length': 1744, 'leftprimer_gc': '44.0', 'leftprimer_start': '11', 'name': '7423548', 'guidecount': 29, 'leftprimer_tm': '60.2216212809', 'product_length': 1578, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCAAGGGAGATATAAGTGAAGAGCT', 'expandedpriming': 20}
{'rightprimer': 'GGGAGGCTAGTAGTGGCTGA', 'rightprimer_start': '2508', 'rightprimer_tm': '60.3972540744', 'leftprimer_length': '24', 'template_length': 2492, 'leftprimer_gc': '33.3333333333', 'leftprimer_start': '58', 'name': '7439366', 'guidecount': 29, 'leftprimer_tm': '56.1574615864', 'product_length': 2450, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TTGATGTAAATACCATCACATCCA', 'expandedpriming': 0}
{'rightprimer': 'GTGGCTCCACTAGAAATTCACTT', 'rightprimer_start': '2217', 'rightprimer_tm': '58.6723198053', 'leftprimer_length': '20', 'template_length': 2200, 'leftprimer_gc': '50.0', 'leftprimer_start': '9', 'name': '10109687', 'guidecount': 29, 'leftprimer_tm': '58.6775577393', 'product_length': 2208, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'GCTCCACAGCAAAGTCCTTT', 'expandedpriming': 0}
{'rightprimer': 'TGCCACTCATCTACAAAGCC', 'rightprimer_start': '2788', 'rightprimer_tm': '57.8861396612', 'leftprimer_length': '24', 'template_length': 3419, 'leftprimer_gc': '37.5', 'leftprimer_start': '22', 'name': '10922817', 'guidecount': 29, 'leftprimer_tm': '57.7404502981', 'product_length': 2766, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AAGTCATATTGAAGGCACTGAAAC', 'expandedpriming': 20}
{'rightprimer': 'CCATCTAGTGGTATTAGCAGGAACT', 'rightprimer_start': '1640', 'rightprimer_tm': '59.6943563472', 'leftprimer_length': '21', 'template_length': 1634, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '42', 'name': '11110543', 'guidecount': 29, 'leftprimer_tm': '59.9964199684', 'product_length': 1598, 'rightprimer_length': '25', 'rightprimer_gc': '44.0', 'leftprimer': 'TCAGTTCATTCACTCCTGGGC', 'expandedpriming': 20}
{'rightprimer': 'AACGCTCCTGCTCAACAATG', 'rightprimer_start': '2172', 'rightprimer_tm': '59.1229293275', 'leftprimer_length': '24', 'template_length': 2249, 'leftprimer_gc': '37.5', 'leftprimer_start': '0', 'name': '11478839', 'guidecount': 29, 'leftprimer_tm': '56.6386273746', 'product_length': 2172, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CAACTAGACTTCCTCATTGTTTCT', 'expandedpriming': 20}
{'rightprimer': 'CTGCTGCACTGAACTGGAGA', 'rightprimer_start': '1676', 'rightprimer_tm': '59.9655235618', 'leftprimer_length': '24', 'template_length': 2362, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '13104202', 'guidecount': 29, 'leftprimer_tm': '60.2022328571', 'product_length': 1676, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGGGAACACTAGTCAGTAATAGCC', 'expandedpriming': 0}
{'rightprimer': 'CTGAGCCGGGAGATATCTGC', 'rightprimer_start': '1954', 'rightprimer_tm': '59.7539135941', 'leftprimer_length': '20', 'template_length': 1951, 'leftprimer_gc': '60.0', 'leftprimer_start': '39', 'name': '13619596', 'guidecount': 29, 'leftprimer_tm': '59.8942215598', 'product_length': 1915, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GATGGACTAGCCCTGTGAGC', 'expandedpriming': 20}
{'rightprimer': 'AGGGAACAAAGGCTCCCATG', 'rightprimer_start': '3089', 'rightprimer_tm': '59.9597955695', 'leftprimer_length': '21', 'template_length': 3214, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '0', 'name': '16133007', 'guidecount': 29, 'leftprimer_tm': '59.4526284825', 'product_length': 3089, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCTTCTTCCAGGCACCATTTG', 'expandedpriming': 0}
{'rightprimer': 'AGCATGGCTTCCTCTGTTCC', 'rightprimer_start': '1511', 'rightprimer_tm': '60.0346704238', 'leftprimer_length': '18', 'template_length': 1511, 'leftprimer_gc': '61.1111111111', 'leftprimer_start': '5', 'name': '17861409', 'guidecount': 29, 'leftprimer_tm': '59.9582380077', 'product_length': 1506, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ATCCTGGCCCTGCTCTGA', 'expandedpriming': 0}
{'rightprimer': 'AGCATTCGACACAACACCCT', 'rightprimer_start': '1532', 'rightprimer_tm': '59.8914152912', 'leftprimer_length': '18', 'template_length': 1552, 'leftprimer_gc': '61.1111111111', 'leftprimer_start': '7', 'name': '18278559', 'guidecount': 29, 'leftprimer_tm': '59.7339445097', 'product_length': 1525, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACTAGCAGCGCCTGTTCC', 'expandedpriming': 0}
{'rightprimer': 'GCCTTCTCAATGGGATTTGCC', 'rightprimer_start': '2383', 'rightprimer_tm': '59.862324775', 'leftprimer_length': '20', 'template_length': 2388, 'leftprimer_gc': '55.0', 'leftprimer_start': '137', 'name': '25500080', 'guidecount': 29, 'leftprimer_tm': '59.8897052273', 'product_length': 2246, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'CCCCAACACACAGATGACCA', 'expandedpriming': 20}
{'rightprimer': 'GGCACCCAGGTCTTAAAGGA', 'rightprimer_start': '3554', 'rightprimer_tm': '59.3034989261', 'leftprimer_length': '23', 'template_length': 3539, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '217', 'name': '25594881', 'guidecount': 29, 'leftprimer_tm': '59.5509818647', 'product_length': 3337, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCACTCTAGCTAACCATCAGTGT', 'expandedpriming': 20}
{'rightprimer': 'CCCTGCCAGCTACAGCATAA', 'rightprimer_start': '1711', 'rightprimer_tm': '59.8188862673', 'leftprimer_length': '20', 'template_length': 1754, 'leftprimer_gc': '55.0', 'leftprimer_start': '246', 'name': '27692197', 'guidecount': 29, 'leftprimer_tm': '60.4609866993', 'product_length': 1465, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCAGCCGCTGTGATTCTAGT', 'expandedpriming': 50}
{'rightprimer': 'AGTCGAAATTAGGCTGCCCT', 'rightprimer_start': '3121', 'rightprimer_tm': '59.0875135479', 'leftprimer_length': '22', 'template_length': 3139, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '185', 'name': '27960741', 'guidecount': 29, 'leftprimer_tm': '58.8268486974', 'product_length': 2936, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGCTGTCTGAGGTATTCCTGAA', 'expandedpriming': 50}
{'rightprimer': 'CCCACTATGCAAGCACGCTA', 'rightprimer_start': '1560', 'rightprimer_tm': '60.4618699968', 'leftprimer_length': '20', 'template_length': 1567, 'leftprimer_gc': '55.0', 'leftprimer_start': '258', 'name': '28358073', 'guidecount': 29, 'leftprimer_tm': '60.0286422729', 'product_length': 1302, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ATGGTGGGATTCCCTAGGCT', 'expandedpriming': 20}
{'rightprimer': 'AGAAAAATCTCCGGTGATCTGAAC', 'rightprimer_start': '1807', 'rightprimer_tm': '59.061774302', 'leftprimer_length': '20', 'template_length': 1799, 'leftprimer_gc': '50.0', 'leftprimer_start': '166', 'name': '32882499', 'guidecount': 29, 'leftprimer_tm': '59.3984417484', 'product_length': 1641, 'rightprimer_length': '24', 'rightprimer_gc': '41.6666666667', 'leftprimer': 'AGCGTAAAGTGCGAAGAGGA', 'expandedpriming': 0}
{'rightprimer': 'TCTCTATCCCTGGGAGTAGAGC', 'rightprimer_start': '2591', 'rightprimer_tm': '59.6228072954', 'leftprimer_length': '20', 'template_length': 2584, 'leftprimer_gc': '55.0', 'leftprimer_start': '400', 'name': '34049356', 'guidecount': 29, 'leftprimer_tm': '59.884848188', 'product_length': 2191, 'rightprimer_length': '22', 'rightprimer_gc': '54.5454545455', 'leftprimer': 'TGCTGACAGAAGGGAGGAGA', 'expandedpriming': 0}
{'rightprimer': 'CGCCTACCCCCATCCAAAAA', 'rightprimer_start': '1535', 'rightprimer_tm': '60.3239076781', 'leftprimer_length': '18', 'template_length': 1786, 'leftprimer_gc': '72.2222222222', 'leftprimer_start': '0', 'name': '34605332', 'guidecount': 29, 'leftprimer_tm': '64.0602362366', 'product_length': 1535, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACCTCCAGCCGGTCGTGG', 'expandedpriming': 0}
{'rightprimer': 'TTGCACACACTAGCAGGAGG', 'rightprimer_start': '2748', 'rightprimer_tm': '59.964683149', 'leftprimer_length': '23', 'template_length': 2741, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '371', 'name': '34686261', 'guidecount': 29, 'leftprimer_tm': '59.6788894288', 'product_length': 2377, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACTTCTCTAAGGAGACACGCAAA', 'expandedpriming': 0}
{'rightprimer': 'TCTCCGCCTAGTCTGTCCAA', 'rightprimer_start': '1804', 'rightprimer_tm': '59.9608196165', 'leftprimer_length': '20', 'template_length': 1799, 'leftprimer_gc': '55.0', 'leftprimer_start': '56', 'name': '35089949', 'guidecount': 29, 'leftprimer_tm': '59.8838574339', 'product_length': 1748, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGCTGAAGGTCTCTGTCCCT', 'expandedpriming': 0}
{'rightprimer': 'ATGGCAAGCAGGGAATGTGA', 'rightprimer_start': '2867', 'rightprimer_tm': '59.9599424387', 'leftprimer_length': '19', 'template_length': 2942, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '5', 'name': '36000116', 'guidecount': 29, 'leftprimer_tm': '60.2285516406', 'product_length': 2862, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GCCCTGGCCTGAACTTTGA', 'expandedpriming': 0}
{'rightprimer': 'CAACATGCCCCGGCCATG', 'rightprimer_start': '1437', 'rightprimer_tm': '61.8405938371', 'leftprimer_length': '20', 'template_length': 1430, 'leftprimer_gc': '55.0', 'leftprimer_start': '2', 'name': '38239154', 'guidecount': 29, 'leftprimer_tm': '60.1789924141', 'product_length': 1435, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'TGCCTGGTTGGTCAGAAAGG', 'expandedpriming': 0}
{'rightprimer': 'GTCTCACAGAGGGGCTTCAC', 'rightprimer_start': '1464', 'rightprimer_tm': '60.0368584804', 'leftprimer_length': '20', 'template_length': 2428, 'leftprimer_gc': '60.0', 'leftprimer_start': '24', 'name': '849605', 'guidecount': 28, 'leftprimer_tm': '60.5315315821', 'product_length': 1440, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CATGCTGCGGTTAGAGAGGG', 'expandedpriming': 20}
{'rightprimer': 'TTCTCTGTCTCCCGGGTGAT', 'rightprimer_start': '478', 'rightprimer_tm': '59.9587620532', 'leftprimer_length': '19', 'template_length': 475, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '5', 'name': '934836', 'guidecount': 28, 'leftprimer_tm': '59.7789791093', 'product_length': 473, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCCCCGGCTAAACTGTCTC', 'expandedpriming': 0}
{'rightprimer': 'ATACCCCCCTGCTAGCCCGG', 'rightprimer_start': '2004', 'rightprimer_tm': '65.5409788543', 'leftprimer_length': '20', 'template_length': 1990, 'leftprimer_gc': '60.0', 'leftprimer_start': '48', 'name': '1184344', 'guidecount': 28, 'leftprimer_tm': '62.0044084134', 'product_length': 1956, 'rightprimer_length': '20', 'rightprimer_gc': '70.0', 'leftprimer': 'GCATCCCTGGCCCAGAGATT', 'expandedpriming': 20}
{'rightprimer': 'GCAGCTGTACTACTGGACATGT', 'rightprimer_start': '1847', 'rightprimer_tm': '60.0939733858', 'leftprimer_length': '20', 'template_length': 1842, 'leftprimer_gc': '55.0', 'leftprimer_start': '373', 'name': '2070236', 'guidecount': 28, 'leftprimer_tm': '60.0376972441', 'product_length': 1474, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'TGCCAGTAACGTACAGCCTG', 'expandedpriming': 0}
{'rightprimer': 'CCTCACCTCAAACCACTGCT', 'rightprimer_start': '3764', 'rightprimer_tm': '59.8906577282', 'leftprimer_length': '20', 'template_length': 3876, 'leftprimer_gc': '60.0', 'leftprimer_start': '893', 'name': '3587765', 'guidecount': 28, 'leftprimer_tm': '59.8931055519', 'product_length': 2871, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGGGTGGAATTAGGCGAGAG', 'expandedpriming': 0}
{'rightprimer': 'CCGCCAGGCCTCAGATGG', 'rightprimer_start': '2739', 'rightprimer_tm': '62.559062545', 'leftprimer_length': '25', 'template_length': 2739, 'leftprimer_gc': '36.0', 'leftprimer_start': '0', 'name': '7019127', 'guidecount': 28, 'leftprimer_tm': '57.7460952745', 'product_length': 2739, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'ACAGTAACCTAGTGTATTCACCAAT', 'expandedpriming': 0}
{'rightprimer': 'TTCTTGTCAGCCACCCGTAC', 'rightprimer_start': '1445', 'rightprimer_tm': '59.9656630029', 'leftprimer_length': '20', 'template_length': 1713, 'leftprimer_gc': '55.0', 'leftprimer_start': '91', 'name': '7279189', 'guidecount': 28, 'leftprimer_tm': '59.8951450311', 'product_length': 1354, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CATAGACTAGGCAGCGGCAA', 'expandedpriming': 20}
{'rightprimer': 'TGGAGCCACTTATTCCACGT', 'rightprimer_start': '3661', 'rightprimer_tm': '59.0188174218', 'leftprimer_length': '20', 'template_length': 3648, 'leftprimer_gc': '55.0', 'leftprimer_start': '396', 'name': '7420485', 'guidecount': 28, 'leftprimer_tm': '59.2155050861', 'product_length': 3265, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACAGGAGTCCCCTACACAGA', 'expandedpriming': 0}
{'rightprimer': 'ACAAGAGGGACAGCGACAAG', 'rightprimer_start': '3431', 'rightprimer_tm': '59.9659413501', 'leftprimer_length': '20', 'template_length': 3532, 'leftprimer_gc': '50.0', 'leftprimer_start': '37', 'name': '9422752', 'guidecount': 28, 'leftprimer_tm': '59.8934785106', 'product_length': 3394, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AAATTGCACAGGGTGTTGGC', 'expandedpriming': 0}
{'rightprimer': 'TGACCAACCTAGCTGGCAAA', 'rightprimer_start': '2356', 'rightprimer_tm': '59.5236617241', 'leftprimer_length': '19', 'template_length': 2359, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '3', 'name': '10305223', 'guidecount': 28, 'leftprimer_tm': '60.0772124829', 'product_length': 2353, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CATTCCAGGCCCCTGTCTG', 'expandedpriming': 0}
{'rightprimer': 'GGGGGAGGTAGTTGAGGAGT', 'rightprimer_start': '3389', 'rightprimer_tm': '59.9571185062', 'leftprimer_length': '20', 'template_length': 3386, 'leftprimer_gc': '55.0', 'leftprimer_start': '222', 'name': '10421704', 'guidecount': 28, 'leftprimer_tm': '59.9701664704', 'product_length': 3167, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCCGCATTACATCCAGCAAG', 'expandedpriming': 0}
{'rightprimer': 'GTACATCAGGGAGCCCATCG', 'rightprimer_start': '3056', 'rightprimer_tm': '59.9659413501', 'leftprimer_length': '21', 'template_length': 3471, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '298', 'name': '13036336', 'guidecount': 28, 'leftprimer_tm': '60.066429946', 'product_length': 2758, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCGATTCACTGGGAGGTAACA', 'expandedpriming': 0}
{'rightprimer': 'TGCACACAAAACCGCAATGA', 'rightprimer_start': '2974', 'rightprimer_tm': '59.5425572328', 'leftprimer_length': '20', 'template_length': 3036, 'leftprimer_gc': '55.0', 'leftprimer_start': '541', 'name': '16435532', 'guidecount': 28, 'leftprimer_tm': '59.9642605059', 'product_length': 2433, 'rightprimer_length': '20', 'rightprimer_gc': '45.0', 'leftprimer': 'GGACGGTACCTTCCTGCAAA', 'expandedpriming': 0}
{'rightprimer': 'AGTTCCATGCCCTAAAGCCA', 'rightprimer_start': '2245', 'rightprimer_tm': '59.2959242932', 'leftprimer_length': '20', 'template_length': 2296, 'leftprimer_gc': '55.0', 'leftprimer_start': '7', 'name': '25356023', 'guidecount': 28, 'leftprimer_tm': '59.466967908', 'product_length': 2238, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACCCTAGTCGCAAAGAGCTC', 'expandedpriming': 50}
{'rightprimer': 'TCTATCTCGCCGGGTACAGT', 'rightprimer_start': '1721', 'rightprimer_tm': '59.8179388436', 'leftprimer_length': '21', 'template_length': 1989, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '8', 'name': '29214541', 'guidecount': 28, 'leftprimer_tm': '59.6085152968', 'product_length': 1713, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CGCTGCGCCACTGATATATTC', 'expandedpriming': 20}
{'rightprimer': 'GTCACATGACCAGGGCTGAC', 'rightprimer_start': '2142', 'rightprimer_tm': '60.6748946543', 'leftprimer_length': '18', 'template_length': 2126, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '0', 'name': '32897536', 'guidecount': 28, 'leftprimer_tm': '60.6695689896', 'product_length': 2142, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCTGGGACAAGCCGTGTG', 'expandedpriming': 0}
{'rightprimer': 'GGGTTGGCATCCTGAAGCTA', 'rightprimer_start': '2407', 'rightprimer_tm': '59.7441096185', 'leftprimer_length': '24', 'template_length': 2417, 'leftprimer_gc': '41.6666666667', 'leftprimer_start': '64', 'name': '34583306', 'guidecount': 28, 'leftprimer_tm': '59.774957522', 'product_length': 2343, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGTGGTTATAGTGGACAATCAGCT', 'expandedpriming': 0}
{'rightprimer': 'TTTCGCTCCCTGTATTCGCA', 'rightprimer_start': '1404', 'rightprimer_tm': '59.7519879697', 'leftprimer_length': '20', 'template_length': 1433, 'leftprimer_gc': '55.0', 'leftprimer_start': '8', 'name': '34775144', 'guidecount': 28, 'leftprimer_tm': '59.7500449976', 'product_length': 1396, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGCAAATGGGTCATTGGGTG', 'expandedpriming': 0}
{'rightprimer': 'GACTGCAGCACGATTCTCCT', 'rightprimer_start': '1703', 'rightprimer_tm': '60.1084244772', 'leftprimer_length': '20', 'template_length': 1738, 'leftprimer_gc': '60.0', 'leftprimer_start': '23', 'name': '35531328', 'guidecount': 28, 'leftprimer_tm': '60.17911589', 'product_length': 1680, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTCATCCGGCACAGACAGAG', 'expandedpriming': 20}
{'rightprimer': 'CCGTCTGCCTCATGTCTTCA', 'rightprimer_start': '2368', 'rightprimer_tm': '59.7525399081', 'leftprimer_length': '20', 'template_length': 2742, 'leftprimer_gc': '55.0', 'leftprimer_start': '1', 'name': '35655470', 'guidecount': 28, 'leftprimer_tm': '59.4019352867', 'product_length': 2367, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GAAATGTGTGCTGGTGGTCC', 'expandedpriming': 0}
{'rightprimer': 'CACAGGCACTTGCTGTTTGT', 'rightprimer_start': '3850', 'rightprimer_tm': '59.542964846', 'leftprimer_length': '20', 'template_length': 3974, 'leftprimer_gc': '55.0', 'leftprimer_start': '282', 'name': '37081956', 'guidecount': 28, 'leftprimer_tm': '59.5955740658', 'product_length': 3568, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGGTTGTAGTTTCCCTGCCT', 'expandedpriming': 0}
{'rightprimer': 'AACTTACCCTGGTGCAGAAC', 'rightprimer_start': '1984', 'rightprimer_tm': '57.7280560547', 'leftprimer_length': '25', 'template_length': 1987, 'leftprimer_gc': '36.0', 'leftprimer_start': '0', 'name': '37203436', 'guidecount': 28, 'leftprimer_tm': '56.3896251296', 'product_length': 1984, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GTAACCAGCTAGTTAATCAGTAGTT', 'expandedpriming': 0}
{'rightprimer': 'CAGGGTTTCGGCCTAAGCTC', 'rightprimer_start': '1981', 'rightprimer_tm': '60.7447709052', 'leftprimer_length': '18', 'template_length': 2024, 'leftprimer_gc': '72.2222222222', 'leftprimer_start': '7', 'name': '336763', 'guidecount': 27, 'leftprimer_tm': '61.1786113794', 'product_length': 1974, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCAGGCTGGGGGGTATCC', 'expandedpriming': 0}
{'rightprimer': 'GCACTGGTGTCACGATTGTT', 'rightprimer_start': '1588', 'rightprimer_tm': '59.0556143835', 'leftprimer_length': '25', 'template_length': 1748, 'leftprimer_gc': '40.0', 'leftprimer_start': '11', 'name': '792522', 'guidecount': 27, 'leftprimer_tm': '57.5290651757', 'product_length': 1577, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGAGACTTTAGGAGTAAAAGAGTCT', 'expandedpriming': 20}
{'rightprimer': 'AGGACCATGGCTTTACCTGC', 'rightprimer_start': '1681', 'rightprimer_tm': '60.0340888334', 'leftprimer_length': '20', 'template_length': 1763, 'leftprimer_gc': '55.0', 'leftprimer_start': '4', 'name': '1146646', 'guidecount': 27, 'leftprimer_tm': '59.9549915989', 'product_length': 1677, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TATCCTAGTGGTTGGGGCCA', 'expandedpriming': 0}
{'rightprimer': 'GAATTCCCCGCTAGGTGGC', 'rightprimer_start': '2987', 'rightprimer_tm': '60.5262245885', 'leftprimer_length': '20', 'template_length': 2979, 'leftprimer_gc': '55.0', 'leftprimer_start': '181', 'name': '1264944', 'guidecount': 27, 'leftprimer_tm': '60.6227379882', 'product_length': 2806, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'ATCTGCATCCCTCCCACTGT', 'expandedpriming': 0}
{'rightprimer': 'GATGGTGCTCCTGGGAAC', 'rightprimer_start': '1417', 'rightprimer_tm': '57.354191147', 'leftprimer_length': '20', 'template_length': 1409, 'leftprimer_gc': '55.0', 'leftprimer_start': '148', 'name': '2363489', 'guidecount': 27, 'leftprimer_tm': '59.6780038877', 'product_length': 1269, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'GCCCGACTTCTTCTGGAACT', 'expandedpriming': 0}
{'rightprimer': 'GGAGTCCTCTGCCCCCTAAA', 'rightprimer_start': '1465', 'rightprimer_tm': '60.6206996901', 'leftprimer_length': '18', 'template_length': 1666, 'leftprimer_gc': '77.7777777778', 'leftprimer_start': '4', 'name': '2386095', 'guidecount': 27, 'leftprimer_tm': '65.4418333702', 'product_length': 1461, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCGGCACCCCCACCCAAG', 'expandedpriming': 0}
{'rightprimer': 'GTTGTTCCTCCAGGTGCTTT', 'rightprimer_start': '2631', 'rightprimer_tm': '58.3075893752', 'leftprimer_length': '20', 'template_length': 2623, 'leftprimer_gc': '50.0', 'leftprimer_start': '82', 'name': '2711870', 'guidecount': 27, 'leftprimer_tm': '59.5175537345', 'product_length': 2549, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGGGCTTGTTCCATCCAGTT', 'expandedpriming': 0}
{'rightprimer': 'TGTGTACTGATGCCGGAGTG', 'rightprimer_start': '3239', 'rightprimer_tm': '59.7536395582', 'leftprimer_length': '24', 'template_length': 3259, 'leftprimer_gc': '41.6666666667', 'leftprimer_start': '17', 'name': '3137826', 'guidecount': 27, 'leftprimer_tm': '59.8348201748', 'product_length': 3222, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGTGTATAATCAGCTTAAGCCCCA', 'expandedpriming': 20}
{'rightprimer': 'AGGAGTAAACTGCCCAAAGA', 'rightprimer_start': '2414', 'rightprimer_tm': '56.3560021144', 'leftprimer_length': '21', 'template_length': 2428, 'leftprimer_gc': '42.8571428571', 'leftprimer_start': '0', 'name': '3256807', 'guidecount': 27, 'leftprimer_tm': '56.1021478763', 'product_length': 2414, 'rightprimer_length': '20', 'rightprimer_gc': '45.0', 'leftprimer': 'AAGACTATCTAGCACACCACA', 'expandedpriming': 0}
{'rightprimer': 'CGAGCCCCGGCACTGAGC', 'rightprimer_start': '2691', 'rightprimer_tm': '65.7904795407', 'leftprimer_length': '20', 'template_length': 2687, 'leftprimer_gc': '65.0', 'leftprimer_start': '5', 'name': '4599524', 'guidecount': 27, 'leftprimer_tm': '61.1244768156', 'product_length': 2686, 'rightprimer_length': '18', 'rightprimer_gc': '77.7777777778', 'leftprimer': 'CCCCTAGGTCTGCCCATCTC', 'expandedpriming': 0}
{'rightprimer': 'CCCCGGGTGTGACAAAAACA', 'rightprimer_start': '1695', 'rightprimer_tm': '61.0381298177', 'leftprimer_length': '23', 'template_length': 1898, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '0', 'name': '4891879', 'guidecount': 27, 'leftprimer_tm': '60.6881260214', 'product_length': 1695, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCCAGGAATGGTGCAATAGCTTA', 'expandedpriming': 20}
{'rightprimer': 'GGGTGGGCAATGATATCCCC', 'rightprimer_start': '915', 'rightprimer_tm': '60.2517871204', 'leftprimer_length': '23', 'template_length': 961, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '44', 'name': '6422727', 'guidecount': 27, 'leftprimer_tm': '59.8708873632', 'product_length': 871, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCATGTAAAGCTAGCAATGCACA', 'expandedpriming': 50}
{'rightprimer': 'TCCCCGGCAGAACGTTAC', 'rightprimer_start': '1138', 'rightprimer_tm': '59.341578039', 'leftprimer_length': '20', 'template_length': 1193, 'leftprimer_gc': '50.0', 'leftprimer_start': '86', 'name': '6696090', 'guidecount': 27, 'leftprimer_tm': '59.4538780864', 'product_length': 1052, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'ACTGTGACCCAAGAACAGCA', 'expandedpriming': 50}
{'rightprimer': 'CCACCGGGGTACATTTCAAAA', 'rightprimer_start': '2084', 'rightprimer_tm': '58.7597205318', 'leftprimer_length': '21', 'template_length': 2091, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '0', 'name': '6911674', 'guidecount': 27, 'leftprimer_tm': '58.8934343995', 'product_length': 2084, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'TCTTCAGCCTGGAGATCACTC', 'expandedpriming': 20}
{'rightprimer': 'AATGCATCAGCCAGCTGGAA', 'rightprimer_start': '3090', 'rightprimer_tm': '60.3239994156', 'leftprimer_length': '21', 'template_length': 3103, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '88', 'name': '8512972', 'guidecount': 27, 'leftprimer_tm': '60.2713251024', 'product_length': 3002, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGTCCGAAATGCATGAGGTGT', 'expandedpriming': 20}
{'rightprimer': 'GGTTTTTGAGGGTGCAGCAG', 'rightprimer_start': '2436', 'rightprimer_tm': '59.967459624', 'leftprimer_length': '20', 'template_length': 2442, 'leftprimer_gc': '55.0', 'leftprimer_start': '31', 'name': '10563534', 'guidecount': 27, 'leftprimer_tm': '59.967459624', 'product_length': 2405, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGCCTTGAAACCAAGCAGTG', 'expandedpriming': 20}
{'rightprimer': 'AAATGCACCCAGTGTTCCCA', 'rightprimer_start': '1423', 'rightprimer_tm': '60.1057603554', 'leftprimer_length': '18', 'template_length': 1447, 'leftprimer_gc': '72.2222222222', 'leftprimer_start': '7', 'name': '11454780', 'guidecount': 27, 'leftprimer_tm': '63.1950948248', 'product_length': 1416, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CCTGGAGGGGCGCATTGG', 'expandedpriming': 0}
{'rightprimer': 'CAGATATAACACCTGGGAGCCA', 'rightprimer_start': '2873', 'rightprimer_tm': '59.2943418645', 'leftprimer_length': '22', 'template_length': 2909, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '71', 'name': '13237648', 'guidecount': 27, 'leftprimer_tm': '58.9521427059', 'product_length': 2802, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'TCATAGTGATCCTTGGATGGCA', 'expandedpriming': 50}
{'rightprimer': 'CTCAAGATCGGCCCACCC', 'rightprimer_start': '2447', 'rightprimer_tm': '59.8094221524', 'leftprimer_length': '22', 'template_length': 2462, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '113', 'name': '15584958', 'guidecount': 27, 'leftprimer_tm': '59.8272825472', 'product_length': 2334, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'ACACTAGGCAACACAGAAGGTT', 'expandedpriming': 20}
{'rightprimer': 'CCACTGGTACTGATGGCCAT', 'rightprimer_start': '2036', 'rightprimer_tm': '59.4519689592', 'leftprimer_length': '21', 'template_length': 2360, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '6', 'name': '20224785', 'guidecount': 27, 'leftprimer_tm': '59.174526392', 'product_length': 2030, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCAGACAGCCAGTAGTAAGA', 'expandedpriming': 20}
{'rightprimer': 'CACTTCTATGACAATGGCCTGC', 'rightprimer_start': '1955', 'rightprimer_tm': '59.6429912857', 'leftprimer_length': '20', 'template_length': 1978, 'leftprimer_gc': '55.0', 'leftprimer_start': '86', 'name': '20309656', 'guidecount': 27, 'leftprimer_tm': '60.0337963284', 'product_length': 1869, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'ATTGATGCACCTCCACCCAG', 'expandedpriming': 50}
{'rightprimer': 'TAGTGAAATCTGGCTCCGGC', 'rightprimer_start': '1616', 'rightprimer_tm': '59.8219317468', 'leftprimer_length': '20', 'template_length': 1628, 'leftprimer_gc': '55.0', 'leftprimer_start': '31', 'name': '21841497', 'guidecount': 27, 'leftprimer_tm': '59.5861679528', 'product_length': 1585, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCAGGGCACTCTAGGAGGTT', 'expandedpriming': 20}
{'rightprimer': 'TGGATACCTGCCTTGGGACT', 'rightprimer_start': '2532', 'rightprimer_tm': '60.2531601443', 'leftprimer_length': '20', 'template_length': 2635, 'leftprimer_gc': '55.0', 'leftprimer_start': '2', 'name': '23684601', 'guidecount': 27, 'leftprimer_tm': '60.179056414', 'product_length': 2530, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TACTGGCCGGATAGCAAAGC', 'expandedpriming': 0}
{'rightprimer': 'CGGGATGCCTAGCTGAGAAG', 'rightprimer_start': '1362', 'rightprimer_tm': '59.9667721369', 'leftprimer_length': '21', 'template_length': 1356, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '67', 'name': '23947737', 'guidecount': 27, 'leftprimer_tm': '59.5726323365', 'product_length': 1295, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TGGCCTGGTGGTCAAGTAATT', 'expandedpriming': 20}
{'rightprimer': 'GTGTTTGGGGGTTGAAGCAC', 'rightprimer_start': '3988', 'rightprimer_tm': '59.8958795713', 'leftprimer_length': '21', 'template_length': 4001, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '278', 'name': '25150610', 'guidecount': 27, 'leftprimer_tm': '59.924626102', 'product_length': 3710, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGGGCTAGTTTGCAATCCAGT', 'expandedpriming': 20}
{'rightprimer': 'CAATAAGCAGCTAGTTCTCATCAG', 'rightprimer_start': '3323', 'rightprimer_tm': '57.4719860876', 'leftprimer_length': '20', 'template_length': 3315, 'leftprimer_gc': '55.0', 'leftprimer_start': '322', 'name': '25471485', 'guidecount': 27, 'leftprimer_tm': '59.3825872816', 'product_length': 3001, 'rightprimer_length': '24', 'rightprimer_gc': '41.6666666667', 'leftprimer': 'ATGAAGTCACTCCTGCCCAG', 'expandedpriming': 0}
{'rightprimer': 'CCATGCATGGGAAGGGGTTA', 'rightprimer_start': '2422', 'rightprimer_tm': '59.7385976368', 'leftprimer_length': '20', 'template_length': 2853, 'leftprimer_gc': '55.0', 'leftprimer_start': '6', 'name': '25941504', 'guidecount': 27, 'leftprimer_tm': '59.3794444032', 'product_length': 2416, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AACTAGGCATGACTGGGGTG', 'expandedpriming': 0}
{'rightprimer': 'GCCGGACAGTATAGCAAGTTTT', 'rightprimer_start': '2780', 'rightprimer_tm': '58.9923653846', 'leftprimer_length': '20', 'template_length': 2781, 'leftprimer_gc': '55.0', 'leftprimer_start': '320', 'name': '27647053', 'guidecount': 27, 'leftprimer_tm': '59.0157696557', 'product_length': 2460, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'GTCCCTGGAATCACACAGGA', 'expandedpriming': 20}
{'rightprimer': 'GTTGCCGGACATAGAGGC', 'rightprimer_start': '2284', 'rightprimer_tm': '57.8734764841', 'leftprimer_length': '20', 'template_length': 2267, 'leftprimer_gc': '50.0', 'leftprimer_start': '1152', 'name': '27708480', 'guidecount': 27, 'leftprimer_tm': '59.5963445507', 'product_length': 1132, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'TTCATGCAACCCCAGCACTA', 'expandedpriming': 0}
{'rightprimer': 'AGATCAAGCACAGGCACCAA', 'rightprimer_start': '2539', 'rightprimer_tm': '59.888938751', 'leftprimer_length': '23', 'template_length': 2628, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '10', 'name': '29773569', 'guidecount': 27, 'leftprimer_tm': '59.4743913227', 'product_length': 2529, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGGGATTTATTAGTGCTGTCCCA', 'expandedpriming': 20}
{'rightprimer': 'TGGCAAAACTAGCATTGGGC', 'rightprimer_start': '1416', 'rightprimer_tm': '59.3923795873', 'leftprimer_length': '21', 'template_length': 1419, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '168', 'name': '33302914', 'guidecount': 27, 'leftprimer_tm': '59.7207286412', 'product_length': 1248, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GTATGTCTCTGGTTGCACCCT', 'expandedpriming': 20}
{'rightprimer': 'CTCAGTTTTCCAGGCACAGAAC', 'rightprimer_start': '1326', 'rightprimer_tm': '59.7082593529', 'leftprimer_length': '20', 'template_length': 1318, 'leftprimer_gc': '55.0', 'leftprimer_start': '210', 'name': '34792656', 'guidecount': 27, 'leftprimer_tm': '59.8131268637', 'product_length': 1116, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'AATGGGGAGGGTCATGATGC', 'expandedpriming': 0}
{'rightprimer': 'TGCTCAGGTCTCTCTAGTGA', 'rightprimer_start': '3838', 'rightprimer_tm': '56.8171532329', 'leftprimer_length': '25', 'template_length': 4359, 'leftprimer_gc': '36.0', 'leftprimer_start': '26', 'name': '35135369', 'guidecount': 27, 'leftprimer_tm': '56.6379144652', 'product_length': 3812, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGATTTATAAGCCATTCCTAATTGC', 'expandedpriming': 20}
{'rightprimer': 'CACTAGTGTCAGGAGACTCATGT', 'rightprimer_start': '842', 'rightprimer_tm': '59.2387821299', 'leftprimer_length': '20', 'template_length': 891, 'leftprimer_gc': '55.0', 'leftprimer_start': '276', 'name': '35700877', 'guidecount': 27, 'leftprimer_tm': '59.4019352867', 'product_length': 566, 'rightprimer_length': '23', 'rightprimer_gc': '47.8260869565', 'leftprimer': 'GCAACCAGTTGACATGTCCC', 'expandedpriming': 50}
{'rightprimer': 'GTATGCCGGCAGTAGAGGG', 'rightprimer_start': '2854', 'rightprimer_tm': '59.6321604177', 'leftprimer_length': '20', 'template_length': 2851, 'leftprimer_gc': '50.0', 'leftprimer_start': '211', 'name': '37586797', 'guidecount': 27, 'leftprimer_tm': '60.0328129766', 'product_length': 2643, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'ATTTACTGGCCATGGCTGCT', 'expandedpriming': 0}
{'rightprimer': 'GTTCCTAGCACACCAGCCTT', 'rightprimer_start': '1695', 'rightprimer_tm': '59.9631254283', 'leftprimer_length': '20', 'template_length': 1693, 'leftprimer_gc': '55.0', 'leftprimer_start': '8', 'name': '2411877', 'guidecount': 26, 'leftprimer_tm': '59.9656630029', 'product_length': 1687, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGGTAACTGGCGTTCTCACC', 'expandedpriming': 20}
{'rightprimer': 'CTAGGGTGTTGGGTGCCC', 'rightprimer_start': '843', 'rightprimer_tm': '59.644884583', 'leftprimer_length': '19', 'template_length': 845, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '9', 'name': '2910451', 'guidecount': 26, 'leftprimer_tm': '59.6987069347', 'product_length': 834, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'CTCCCTCTGCCTTTGTCCC', 'expandedpriming': 20}
{'rightprimer': 'AGAGACGTGTTTGGCAGCAT', 'rightprimer_start': '1519', 'rightprimer_tm': '60.2504670515', 'leftprimer_length': '20', 'template_length': 1790, 'leftprimer_gc': '55.0', 'leftprimer_start': '90', 'name': '3012752', 'guidecount': 26, 'leftprimer_tm': '60.0340888334', 'product_length': 1429, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AGCTGGGATTAGGGTTGCAC', 'expandedpriming': 0}
{'rightprimer': 'CCGCCTCTGGAATGATGTCA', 'rightprimer_start': '3342', 'rightprimer_tm': '59.8212334702', 'leftprimer_length': '20', 'template_length': 3332, 'leftprimer_gc': '55.0', 'leftprimer_start': '85', 'name': '3186964', 'guidecount': 26, 'leftprimer_tm': '59.4764810721', 'product_length': 3257, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCAACTGCTTTACTCAGGGC', 'expandedpriming': 0}
{'rightprimer': 'GGGTCTCCCATGGCATTTACT', 'rightprimer_start': '1285', 'rightprimer_tm': '59.7845772581', 'leftprimer_length': '20', 'template_length': 1275, 'leftprimer_gc': '55.0', 'leftprimer_start': '146', 'name': '3487382', 'guidecount': 26, 'leftprimer_tm': '59.8975186744', 'product_length': 1139, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'ACCTGGCGCGTGTAATAGAG', 'expandedpriming': 20}
{'rightprimer': 'GCTACGACCTGCTTCCCATT', 'rightprimer_start': '2701', 'rightprimer_tm': '60.107654381', 'leftprimer_length': '20', 'template_length': 2827, 'leftprimer_gc': '55.0', 'leftprimer_start': '19', 'name': '4058609', 'guidecount': 26, 'leftprimer_tm': '60.3219192968', 'product_length': 2682, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGATTGGCTGACTGAGTGCC', 'expandedpriming': 0}
{'rightprimer': 'CTGACTCATACCGGGGCC', 'rightprimer_start': '1483', 'rightprimer_tm': '59.1776010959', 'leftprimer_length': '20', 'template_length': 1468, 'leftprimer_gc': '55.0', 'leftprimer_start': '40', 'name': '4441426', 'guidecount': 26, 'leftprimer_tm': '59.6687217718', 'product_length': 1443, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'CCCCCAACAAGCTGATGGTA', 'expandedpriming': 0}
{'rightprimer': 'GGAAATCCTGGTGCTTCCCA', 'rightprimer_start': '1684', 'rightprimer_tm': '59.9603818904', 'leftprimer_length': '20', 'template_length': 1679, 'leftprimer_gc': '55.0', 'leftprimer_start': '5', 'name': '4875973', 'guidecount': 26, 'leftprimer_tm': '59.9656630029', 'product_length': 1679, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTACACAGGAACCGTTGGA', 'expandedpriming': 20}
{'rightprimer': 'ACAAAGCGGCTTCTCTGTGG', 'rightprimer_start': '939', 'rightprimer_tm': '60.8864901394', 'leftprimer_length': '18', 'template_length': 1586, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '294', 'name': '5408009', 'guidecount': 26, 'leftprimer_tm': '61.4061997848', 'product_length': 645, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCAGGCAGCCTACCAGCT', 'expandedpriming': 20}
{'rightprimer': 'GGTGGACCCTGCAGCTATG', 'rightprimer_start': '6134', 'rightprimer_tm': '60.1530863724', 'leftprimer_length': '21', 'template_length': 6145, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '1', 'name': '5612978', 'guidecount': 26, 'leftprimer_tm': '59.9979126065', 'product_length': 6133, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'CTTCCAGCCGGTAAGTTCTGT', 'expandedpriming': 20}
{'rightprimer': 'TCACTAGTGAAGGGGGGGTA', 'rightprimer_start': '1351', 'rightprimer_tm': '58.9048697236', 'leftprimer_length': '22', 'template_length': 1359, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '24', 'name': '5675130', 'guidecount': 26, 'leftprimer_tm': '58.8349288252', 'product_length': 1327, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCATTTCAAAAGCTAGGGGTGT', 'expandedpriming': 20}
{'rightprimer': 'CCTTGATTCCGGGTGTCTGG', 'rightprimer_start': '2188', 'rightprimer_tm': '60.392997683', 'leftprimer_length': '19', 'template_length': 2178, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '425', 'name': '8777897', 'guidecount': 26, 'leftprimer_tm': '60.0788478813', 'product_length': 1763, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCCAAGATGGACAGCGAGT', 'expandedpriming': 0}
{'rightprimer': 'CAGATGGGAGGAAGGTGTCA', 'rightprimer_start': '3332', 'rightprimer_tm': '58.7205035226', 'leftprimer_length': '21', 'template_length': 3409, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '907', 'name': '9235087', 'guidecount': 26, 'leftprimer_tm': '58.5018329023', 'product_length': 2425, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGCAGTATCCTAATGGCTGTG', 'expandedpriming': 0}
{'rightprimer': 'GCAGGTATGTCTAGGTATACCTT', 'rightprimer_start': '1695', 'rightprimer_tm': '56.6760376182', 'leftprimer_length': '20', 'template_length': 1687, 'leftprimer_gc': '50.0', 'leftprimer_start': '64', 'name': '9287573', 'guidecount': 26, 'leftprimer_tm': '57.3141504193', 'product_length': 1631, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'GCCGGGAACAATACTACACT', 'expandedpriming': 20}
{'rightprimer': 'GTCGCCGGGACTCGCTGA', 'rightprimer_start': '2487', 'rightprimer_tm': '64.4214316345', 'leftprimer_length': '20', 'template_length': 2473, 'leftprimer_gc': '60.0', 'leftprimer_start': '269', 'name': '9438785', 'guidecount': 26, 'leftprimer_tm': '61.2718400868', 'product_length': 2218, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'GGCACTGTACCAGATGGGGA', 'expandedpriming': 0}
{'rightprimer': 'CTGCCATGCCTATAGGTGCA', 'rightprimer_start': '1410', 'rightprimer_tm': '59.8908474873', 'leftprimer_length': '20', 'template_length': 1874, 'leftprimer_gc': '55.0', 'leftprimer_start': '16', 'name': '9725047', 'guidecount': 26, 'leftprimer_tm': '59.6095083763', 'product_length': 1394, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCATTCCCTTTATGCCAGC', 'expandedpriming': 0}
{'rightprimer': 'GTGGACGCCAAGCAACTTTT', 'rightprimer_start': '3897', 'rightprimer_tm': '59.8976996438', 'leftprimer_length': '20', 'template_length': 4334, 'leftprimer_gc': '50.0', 'leftprimer_start': '16', 'name': '10805042', 'guidecount': 26, 'leftprimer_tm': '59.8091850549', 'product_length': 3881, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AGCCTGGTTTGTCCCCATTT', 'expandedpriming': 20}
{'rightprimer': 'GGAAAAACCCAGGTACTACCAC', 'rightprimer_start': '964', 'rightprimer_tm': '58.5847884529', 'leftprimer_length': '20', 'template_length': 948, 'leftprimer_gc': '50.0', 'leftprimer_start': '2', 'name': '14627891', 'guidecount': 26, 'leftprimer_tm': '58.7079153481', 'product_length': 962, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'ATTAGGCCGGTTACTTGGGT', 'expandedpriming': 0}
{'rightprimer': 'GGGGTTGCAGTGGACCTTAA', 'rightprimer_start': '2431', 'rightprimer_tm': '59.8883612627', 'leftprimer_length': '20', 'template_length': 2473, 'leftprimer_gc': '55.0', 'leftprimer_start': '492', 'name': '17454099', 'guidecount': 26, 'leftprimer_tm': '60.035055639', 'product_length': 1939, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CGAGTGGGCCCTCATTTTCT', 'expandedpriming': 50}
{'rightprimer': 'GTGCCAGAGTTAGCCTGGTT', 'rightprimer_start': '3176', 'rightprimer_tm': '59.9631254283', 'leftprimer_length': '20', 'template_length': 3192, 'leftprimer_gc': '60.0', 'leftprimer_start': '46', 'name': '17631459', 'guidecount': 26, 'leftprimer_tm': '60.1063183054', 'product_length': 3130, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACCCCAGCGGTACCTACTAG', 'expandedpriming': 50}
{'rightprimer': 'CCCAGCTCAGTTTCCATGGT', 'rightprimer_start': '2868', 'rightprimer_tm': '59.9612556273', 'leftprimer_length': '20', 'template_length': 2945, 'leftprimer_gc': '55.0', 'leftprimer_start': '156', 'name': '18446965', 'guidecount': 26, 'leftprimer_tm': '59.9626967358', 'product_length': 2712, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CACTGTGGCCCTCTTGTGAT', 'expandedpriming': 0}
{'rightprimer': 'TGCCCTAACTTCCACGTCAA', 'rightprimer_start': '3026', 'rightprimer_tm': '59.2400412576', 'leftprimer_length': '20', 'template_length': 3348, 'leftprimer_gc': '50.0', 'leftprimer_start': '91', 'name': '19956471', 'guidecount': 26, 'leftprimer_tm': '58.6336814162', 'product_length': 2935, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AGCAGAGGAACAGGAATGGT', 'expandedpriming': 0}
{'rightprimer': 'TCCAAGAGTAGTCATTGTACTCCA', 'rightprimer_start': '2476', 'rightprimer_tm': '58.6697666957', 'leftprimer_length': '20', 'template_length': 2867, 'leftprimer_gc': '45.0', 'leftprimer_start': '109', 'name': '21742784', 'guidecount': 26, 'leftprimer_tm': '59.0513219997', 'product_length': 2367, 'rightprimer_length': '24', 'rightprimer_gc': '41.6666666667', 'leftprimer': 'AACTTTGGCACATACGCGTT', 'expandedpriming': 0}
{'rightprimer': 'CGTGCAGAGATTCTTAGAGCGA', 'rightprimer_start': '2289', 'rightprimer_tm': '60.2233633633', 'leftprimer_length': '20', 'template_length': 2493, 'leftprimer_gc': '55.0', 'leftprimer_start': '183', 'name': '21862253', 'guidecount': 26, 'leftprimer_tm': '60.3202467794', 'product_length': 2106, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'CAGACGAAGGCAGCTTGTCT', 'expandedpriming': 0}
{'rightprimer': 'CAGCTGGCACAATGAGCTG', 'rightprimer_start': '2349', 'rightprimer_tm': '59.4932823037', 'leftprimer_length': '20', 'template_length': 2449, 'leftprimer_gc': '50.0', 'leftprimer_start': '366', 'name': '26057962', 'guidecount': 26, 'leftprimer_tm': '59.6135244914', 'product_length': 1983, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'CTGCAGCCTGAACAAAGCAA', 'expandedpriming': 0}
{'rightprimer': 'CTGTAGTGCAAAAGTATCAGCGT', 'rightprimer_start': '1912', 'rightprimer_tm': '59.3200199367', 'leftprimer_length': '20', 'template_length': 1925, 'leftprimer_gc': '60.0', 'leftprimer_start': '8', 'name': '27477277', 'guidecount': 26, 'leftprimer_tm': '59.7494866436', 'product_length': 1904, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'CTAGACTGGCCACCTGGAAC', 'expandedpriming': 20}
{'rightprimer': 'TCATGCTCACATTGCTGGAGA', 'rightprimer_start': '2015', 'rightprimer_tm': '59.7201544188', 'leftprimer_length': '18', 'template_length': 2001, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '435', 'name': '28277494', 'guidecount': 26, 'leftprimer_tm': '59.8075573681', 'product_length': 1580, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'CCTCCCCATGCAGCTCTG', 'expandedpriming': 0}
{'rightprimer': 'CATGGGCAAAGCTGGAAACC', 'rightprimer_start': '3721', 'rightprimer_tm': '60.0370457062', 'leftprimer_length': '20', 'template_length': 3708, 'leftprimer_gc': '55.0', 'leftprimer_start': '334', 'name': '28818251', 'guidecount': 26, 'leftprimer_tm': '60.0373256492', 'product_length': 3387, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CACTCTAAGCAGGCAACCGA', 'expandedpriming': 0}
{'rightprimer': 'CTGGCCCGGCTGCTGTTT', 'rightprimer_start': '1810', 'rightprimer_tm': '63.3473284649', 'leftprimer_length': '20', 'template_length': 1796, 'leftprimer_gc': '55.0', 'leftprimer_start': '110', 'name': '28921517', 'guidecount': 26, 'leftprimer_tm': '60.5489258009', 'product_length': 1700, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'TGAGGGACCAGCCACCAATA', 'expandedpriming': 0}
{'rightprimer': 'TCATTTGCAAAGCCACGGTG', 'rightprimer_start': '3845', 'rightprimer_tm': '59.96814276', 'leftprimer_length': '21', 'template_length': 3874, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '23', 'name': '29114208', 'guidecount': 26, 'leftprimer_tm': '59.995722923', 'product_length': 3822, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GTTGAATAGGGCACTGGGACA', 'expandedpriming': 0}
{'rightprimer': 'GTTGCACGTTTCTGTACACACA', 'rightprimer_start': '1882', 'rightprimer_tm': '59.9054246043', 'leftprimer_length': '20', 'template_length': 2042, 'leftprimer_gc': '50.0', 'leftprimer_start': '36', 'name': '29903519', 'guidecount': 26, 'leftprimer_tm': '59.8953290178', 'product_length': 1846, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'TTTGGCTGGTTTTGAGCAGC', 'expandedpriming': 0}
{'rightprimer': 'GGGCAAGGGAGCTGAGAAAT', 'rightprimer_start': '3577', 'rightprimer_tm': '60.0336985718', 'leftprimer_length': '20', 'template_length': 3711, 'leftprimer_gc': '60.0', 'leftprimer_start': '596', 'name': '30439705', 'guidecount': 26, 'leftprimer_tm': '60.1087564877', 'product_length': 2981, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTTCTCCCACCAGTACGTC', 'expandedpriming': 0}
{'rightprimer': 'AATCCTGGCTGCTTCTAGGC', 'rightprimer_start': '2363', 'rightprimer_tm': '59.8177012175', 'leftprimer_length': '20', 'template_length': 2356, 'leftprimer_gc': '55.0', 'leftprimer_start': '344', 'name': '30851081', 'guidecount': 26, 'leftprimer_tm': '59.9688215861', 'product_length': 2019, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTGCAAACCCACTGCTTCAG', 'expandedpriming': 20}
{'rightprimer': 'CGTGCCCGACTTTACCCATA', 'rightprimer_start': '2357', 'rightprimer_tm': '59.8237807187', 'leftprimer_length': '20', 'template_length': 2409, 'leftprimer_gc': '50.0', 'leftprimer_start': '5', 'name': '31167090', 'guidecount': 26, 'leftprimer_tm': '59.8870049386', 'product_length': 2352, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACAGAATGGCCCACACACAT', 'expandedpriming': 0}
{'rightprimer': 'CCAAAAGCCACATGGGGATG', 'rightprimer_start': '3743', 'rightprimer_tm': '59.4618819025', 'leftprimer_length': '20', 'template_length': 3753, 'leftprimer_gc': '55.0', 'leftprimer_start': '23', 'name': '33772618', 'guidecount': 26, 'leftprimer_tm': '59.6763710045', 'product_length': 3720, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTCTTCCAACGCTACCCCTG', 'expandedpriming': 20}
{'rightprimer': 'GCCCAAGTCTCGGAAGGAAA', 'rightprimer_start': '996', 'rightprimer_tm': '59.9636944336', 'leftprimer_length': '20', 'template_length': 1362, 'leftprimer_gc': '55.0', 'leftprimer_start': '153', 'name': '35854205', 'guidecount': 26, 'leftprimer_tm': '59.893850512', 'product_length': 843, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AAGGTTGGTCGGTGTCTGAC', 'expandedpriming': 0}
{'rightprimer': 'TTCACCGGAAAACAGCTTGT', 'rightprimer_start': '3384', 'rightprimer_tm': '58.2423176923', 'leftprimer_length': '25', 'template_length': 3516, 'leftprimer_gc': '40.0', 'leftprimer_start': '21', 'name': '36241835', 'guidecount': 26, 'leftprimer_tm': '57.5318589741', 'product_length': 3363, 'rightprimer_length': '20', 'rightprimer_gc': '45.0', 'leftprimer': 'CAGATAATCCCTAGCAGTACATACA', 'expandedpriming': 20}
{'rightprimer': 'TCTATAAAGCCGGCCCAATGT', 'rightprimer_start': '1187', 'rightprimer_tm': '59.506977668', 'leftprimer_length': '20', 'template_length': 1377, 'leftprimer_gc': '60.0', 'leftprimer_start': '286', 'name': '37436079', 'guidecount': 26, 'leftprimer_tm': '59.818176161', 'product_length': 901, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'CAATTACCCCCCACCTACCG', 'expandedpriming': 20}
{'rightprimer': 'AAAGCCGGCGATGTCCAATA', 'rightprimer_start': '2758', 'rightprimer_tm': '60.1074104683', 'leftprimer_length': '20', 'template_length': 2756, 'leftprimer_gc': '55.0', 'leftprimer_start': '14', 'name': '139304', 'guidecount': 25, 'leftprimer_tm': '60.1103733516', 'product_length': 2744, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GAACACCAAACGCGTCATCC', 'expandedpriming': 20}
{'rightprimer': 'TAATACCCACCAATTGCCCC', 'rightprimer_start': '802', 'rightprimer_tm': '57.5369534725', 'leftprimer_length': '20', 'template_length': 793, 'leftprimer_gc': '55.0', 'leftprimer_start': '6', 'name': '400145', 'guidecount': 25, 'leftprimer_tm': '57.3306996491', 'product_length': 796, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CCATATCTCCGGCATCTCTG', 'expandedpriming': 20}
{'rightprimer': 'AGGGTGCCCTGGGGTGCC', 'rightprimer_start': '658', 'rightprimer_tm': '66.8857041333', 'leftprimer_length': '20', 'template_length': 652, 'leftprimer_gc': '60.0', 'leftprimer_start': '11', 'name': '673075', 'guidecount': 25, 'leftprimer_tm': '62.012901798', 'product_length': 647, 'rightprimer_length': '18', 'rightprimer_gc': '77.7777777778', 'leftprimer': 'GGGGCAATGAGCTGCATCAC', 'expandedpriming': 0}
{'rightprimer': 'TCTCCCACCCAACCTTCTCT', 'rightprimer_start': '2264', 'rightprimer_tm': '59.8064260097', 'leftprimer_length': '21', 'template_length': 2259, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '12', 'name': '1883823', 'guidecount': 25, 'leftprimer_tm': '60.2031869145', 'product_length': 2252, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGGAGAGTTAGCTGTGGACCT', 'expandedpriming': 20}
{'rightprimer': 'GGGTCAATAATGGGTGGGCA', 'rightprimer_start': '1175', 'rightprimer_tm': '60.0326147522', 'leftprimer_length': '20', 'template_length': 1604, 'leftprimer_gc': '55.0', 'leftprimer_start': '15', 'name': '1935643', 'guidecount': 25, 'leftprimer_tm': '60.2511646866', 'product_length': 1160, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCCCAAAGCTAACACTCCA', 'expandedpriming': 20}
{'rightprimer': 'GATATCTTGGTGCCTTCAGTAAAG', 'rightprimer_start': '1201', 'rightprimer_tm': '57.3892585113', 'leftprimer_length': '20', 'template_length': 1199, 'leftprimer_gc': '55.0', 'leftprimer_start': '1', 'name': '2597583', 'guidecount': 25, 'leftprimer_tm': '60.3230873932', 'product_length': 1200, 'rightprimer_length': '24', 'rightprimer_gc': '41.6666666667', 'leftprimer': 'TACAGACGCTCCAGGCTGTA', 'expandedpriming': 20}
{'rightprimer': 'GAGAGTGGGAATTGCCACCA', 'rightprimer_start': '1589', 'rightprimer_tm': '59.9618343238', 'leftprimer_length': '20', 'template_length': 1644, 'leftprimer_gc': '55.0', 'leftprimer_start': '42', 'name': '3095195', 'guidecount': 25, 'leftprimer_tm': '60.0332078652', 'product_length': 1547, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGGATGGCAAGATGGAACCA', 'expandedpriming': 50}
{'rightprimer': 'TGCTAGTTTCAGATGTAGCCAAA', 'rightprimer_start': '1089', 'rightprimer_tm': '58.094571143', 'leftprimer_length': '20', 'template_length': 1098, 'leftprimer_gc': '60.0', 'leftprimer_start': '17', 'name': '3158995', 'guidecount': 25, 'leftprimer_tm': '59.1791287897', 'product_length': 1072, 'rightprimer_length': '23', 'rightprimer_gc': '39.1304347826', 'leftprimer': 'GCCTCAGTCTGTAGGTCTGG', 'expandedpriming': 20}
{'rightprimer': 'GCCCTCTCCTCTGAAATGGC', 'rightprimer_start': '1593', 'rightprimer_tm': '60.4652784092', 'leftprimer_length': '20', 'template_length': 1810, 'leftprimer_gc': '55.0', 'leftprimer_start': '6', 'name': '3579691', 'guidecount': 25, 'leftprimer_tm': '60.2526963904', 'product_length': 1587, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'ATCTAGGGTGGCCTGTGTGA', 'expandedpriming': 0}
{'rightprimer': 'TTTCTCTCGGCAACAGGGTC', 'rightprimer_start': '1498', 'rightprimer_tm': '59.9651041645', 'leftprimer_length': '18', 'template_length': 1942, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '7', 'name': '3905750', 'guidecount': 25, 'leftprimer_tm': '60.0468919059', 'product_length': 1491, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCAGGCGGTTGAGGTCAG', 'expandedpriming': 0}
{'rightprimer': 'CACCCATCTGTGCCCAAGG', 'rightprimer_start': '2123', 'rightprimer_tm': '60.6799772818', 'leftprimer_length': '20', 'template_length': 2132, 'leftprimer_gc': '55.0', 'leftprimer_start': '101', 'name': '5489525', 'guidecount': 25, 'leftprimer_tm': '60.814009712', 'product_length': 2022, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'GGTGTGCGTGGTGTCTCTTT', 'expandedpriming': 20}
{'rightprimer': 'ACCAGGAAGCTGCTTATCGT', 'rightprimer_start': '3186', 'rightprimer_tm': '59.0946430568', 'leftprimer_length': '22', 'template_length': 3248, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '142', 'name': '5534003', 'guidecount': 25, 'leftprimer_tm': '58.8145452874', 'product_length': 3044, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AGGCCCTAATCTCTGTGTCTTT', 'expandedpriming': 50}
{'rightprimer': 'TATAGCGCTTCATCCTGGGC', 'rightprimer_start': '3871', 'rightprimer_tm': '59.6783292029', 'leftprimer_length': '20', 'template_length': 3886, 'leftprimer_gc': '55.0', 'leftprimer_start': '1214', 'name': '5694470', 'guidecount': 25, 'leftprimer_tm': '59.7403531493', 'product_length': 2657, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CATCCTGGCTCCCCATTTCA', 'expandedpriming': 20}
{'rightprimer': 'GGGGATAACGTTGTGGTCTGT', 'rightprimer_start': '2569', 'rightprimer_tm': '59.9972266699', 'leftprimer_length': '20', 'template_length': 2608, 'leftprimer_gc': '55.0', 'leftprimer_start': '98', 'name': '6029313', 'guidecount': 25, 'leftprimer_tm': '59.7429605852', 'product_length': 2471, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'CCCAAAGGCTAGATGCAGGT', 'expandedpriming': 50}
{'rightprimer': 'ACAGCAACTCCAACTGGTCG', 'rightprimer_start': '1468', 'rightprimer_tm': '60.5328619766', 'leftprimer_length': '20', 'template_length': 1478, 'leftprimer_gc': '60.0', 'leftprimer_start': '0', 'name': '6290548', 'guidecount': 25, 'leftprimer_tm': '60.1790618901', 'product_length': 1468, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCTCTCGCATCCAGGGAAAG', 'expandedpriming': 20}
{'rightprimer': 'TGCAGCGAATGTTAAACTCGC', 'rightprimer_start': '1540', 'rightprimer_tm': '60.136084716', 'leftprimer_length': '20', 'template_length': 1537, 'leftprimer_gc': '50.0', 'leftprimer_start': '5', 'name': '6662684', 'guidecount': 25, 'leftprimer_tm': '59.7606526767', 'product_length': 1535, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'GCAGCACGCAGACAAGAAAT', 'expandedpriming': 20}
{'rightprimer': 'TCCTAGAGAATACCCATGCAAAA', 'rightprimer_start': '2940', 'rightprimer_tm': '57.2912636647', 'leftprimer_length': '20', 'template_length': 2940, 'leftprimer_gc': '60.0', 'leftprimer_start': '410', 'name': '7171669', 'guidecount': 25, 'leftprimer_tm': '59.6750570803', 'product_length': 2530, 'rightprimer_length': '23', 'rightprimer_gc': '39.1304347826', 'leftprimer': 'CACCTAGGTTGTGGGGACAG', 'expandedpriming': 20}
{'rightprimer': 'GGCCAAGCATCCTAGAGTGG', 'rightprimer_start': '1436', 'rightprimer_tm': '60.1790343683', 'leftprimer_length': '20', 'template_length': 1976, 'leftprimer_gc': '60.0', 'leftprimer_start': '11', 'name': '7247766', 'guidecount': 25, 'leftprimer_tm': '60.250814066', 'product_length': 1425, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CGAGCCTAGATCCCTACGGT', 'expandedpriming': 20}
{'rightprimer': 'GGTATGCAGTATCCCCTCCC', 'rightprimer_start': '1383', 'rightprimer_tm': '59.0150052198', 'leftprimer_length': '20', 'template_length': 1568, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '9419416', 'guidecount': 25, 'leftprimer_tm': '58.1285824134', 'product_length': 1383, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'AGCTAGCTTGACCTCATGGA', 'expandedpriming': 0}
{'rightprimer': 'ACTCAGTAGAAGCTGCTGCAG', 'rightprimer_start': '2897', 'rightprimer_tm': '60.0670003553', 'leftprimer_length': '20', 'template_length': 2892, 'leftprimer_gc': '50.0', 'leftprimer_start': '13', 'name': '10286034', 'guidecount': 25, 'leftprimer_tm': '59.8870049386', 'product_length': 2884, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'TGCTTTTCCTGTGATGGCCT', 'expandedpriming': 20}
{'rightprimer': 'ACAGACGACAGCCAGATGTG', 'rightprimer_start': '2913', 'rightprimer_tm': '60.0378823331', 'leftprimer_length': '20', 'template_length': 2984, 'leftprimer_gc': '60.0', 'leftprimer_start': '6', 'name': '10686022', 'guidecount': 25, 'leftprimer_tm': '59.7350445361', 'product_length': 2907, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTCTAGGGCCCTGTCCCTTA', 'expandedpriming': 0}
{'rightprimer': 'TGCAAGCAGGTACCAGGAGA', 'rightprimer_start': '1586', 'rightprimer_tm': '60.8359712156', 'leftprimer_length': '18', 'template_length': 1860, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '0', 'name': '11941660', 'guidecount': 25, 'leftprimer_tm': '62.278513166', 'product_length': 1586, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCAAGTTGCCGGGGGGTG', 'expandedpriming': 20}
{'rightprimer': 'GGGGTTACAAAGACCTGCCT', 'rightprimer_start': '1621', 'rightprimer_tm': '59.5955740658', 'leftprimer_length': '20', 'template_length': 1729, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '13590326', 'guidecount': 25, 'leftprimer_tm': '59.3171484107', 'product_length': 1621, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGTACTAGCGTGCCCACAAA', 'expandedpriming': 0}
{'rightprimer': 'AACACAAATACTAGCACTTCCTTT', 'rightprimer_start': '1765', 'rightprimer_tm': '56.7940356441', 'leftprimer_length': '20', 'template_length': 1757, 'leftprimer_gc': '50.0', 'leftprimer_start': '23', 'name': '13856112', 'guidecount': 25, 'leftprimer_tm': '57.2125270574', 'product_length': 1742, 'rightprimer_length': '24', 'rightprimer_gc': '33.3333333333', 'leftprimer': 'CCAGTCCAATGGCAGAAGTA', 'expandedpriming': 0}
{'rightprimer': 'GCAAATAGCCTTTGAACCCC', 'rightprimer_start': '3359', 'rightprimer_tm': '57.327038562', 'leftprimer_length': '22', 'template_length': 3409, 'leftprimer_gc': '40.9090909091', 'leftprimer_start': '2', 'name': '16707017', 'guidecount': 25, 'leftprimer_tm': '57.3993075783', 'product_length': 3357, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGTTCTCTAGCACTTGTAGCAA', 'expandedpriming': 0}
{'rightprimer': 'GGTTGTCCTGGTGCTTCTGA', 'rightprimer_start': '2295', 'rightprimer_tm': '59.8914152912', 'leftprimer_length': '23', 'template_length': 2731, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '17', 'name': '17130106', 'guidecount': 25, 'leftprimer_tm': '59.8673542586', 'product_length': 2278, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGAGCATTAGACTTCTGCAGAGG', 'expandedpriming': 20}
{'rightprimer': 'CACTTGTAACGCAGGCACG', 'rightprimer_start': '1291', 'rightprimer_tm': '59.795938075', 'leftprimer_length': '19', 'template_length': 1300, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '7', 'name': '18088464', 'guidecount': 25, 'leftprimer_tm': '59.7754075638', 'product_length': 1284, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'CCCCGGACCATTTCCAGAG', 'expandedpriming': 20}
{'rightprimer': 'CCGACTCAATGGTTCCAGGT', 'rightprimer_start': '2481', 'rightprimer_tm': '59.6750570803', 'leftprimer_length': '20', 'template_length': 2466, 'leftprimer_gc': '50.0', 'leftprimer_start': '74', 'name': '18121523', 'guidecount': 25, 'leftprimer_tm': '59.6734050663', 'product_length': 2407, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGCGAGTGCCCCAAATCTAA', 'expandedpriming': 0}
{'rightprimer': 'TATTTGTGCAGGAAGCCCCA', 'rightprimer_start': '1597', 'rightprimer_tm': '59.5924717926', 'leftprimer_length': '20', 'template_length': 2267, 'leftprimer_gc': '55.0', 'leftprimer_start': '4', 'name': '18181328', 'guidecount': 25, 'leftprimer_tm': '59.1693519361', 'product_length': 1593, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CAGCCGGAGATAGTGGGAAA', 'expandedpriming': 0}
{'rightprimer': 'GGTCTGTGCTAGTCTTCTGCA', 'rightprimer_start': '2247', 'rightprimer_tm': '59.7263935161', 'leftprimer_length': '20', 'template_length': 2250, 'leftprimer_gc': '50.0', 'leftprimer_start': '16', 'name': '18432712', 'guidecount': 25, 'leftprimer_tm': '59.3994430857', 'product_length': 2231, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'CCGCACAAAGGCCAATAACA', 'expandedpriming': 20}
{'rightprimer': 'GCTGACGCTGGAGTTGATCA', 'rightprimer_start': '2518', 'rightprimer_tm': '60.3903403739', 'leftprimer_length': '18', 'template_length': 2734, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '24', 'name': '18492190', 'guidecount': 25, 'leftprimer_tm': '61.699894386', 'product_length': 2494, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTGCTGACCTGAGCCCGT', 'expandedpriming': 20}
{'rightprimer': 'CACTGCATGATTCAGAGCTGC', 'rightprimer_start': '2466', 'rightprimer_tm': '59.9357917216', 'leftprimer_length': '22', 'template_length': 2649, 'leftprimer_gc': '54.5454545455', 'leftprimer_start': '2', 'name': '19124197', 'guidecount': 25, 'leftprimer_tm': '62.6361691613', 'product_length': 2464, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'TGGGATTACCGGCTTGTGTAGC', 'expandedpriming': 0}
{'rightprimer': 'ACATATTATCTTGCGATCATTGTGG', 'rightprimer_start': '2114', 'rightprimer_tm': '57.8625099687', 'leftprimer_length': '20', 'template_length': 2125, 'leftprimer_gc': '50.0', 'leftprimer_start': '292', 'name': '19880171', 'guidecount': 25, 'leftprimer_tm': '57.9701617483', 'product_length': 1822, 'rightprimer_length': '25', 'rightprimer_gc': '36.0', 'leftprimer': 'CTAGAAGTCAGGCGGCATTT', 'expandedpriming': 20}
{'rightprimer': 'GGCTCACAGACACGTGGAAT', 'rightprimer_start': '1467', 'rightprimer_tm': '60.3207705649', 'leftprimer_length': '21', 'template_length': 1612, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '10', 'name': '20499093', 'guidecount': 25, 'leftprimer_tm': '60.1336681163', 'product_length': 1457, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGTCTTCCTTTGCAAGCCTGT', 'expandedpriming': 20}
{'rightprimer': 'AGTCTGACTGGACCTTGGGT', 'rightprimer_start': '3907', 'rightprimer_tm': '60.1049342064', 'leftprimer_length': '18', 'template_length': 4021, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '2', 'name': '23447536', 'guidecount': 25, 'leftprimer_tm': '60.1249424037', 'product_length': 3905, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTGTGGACCTAGCTGCC', 'expandedpriming': 0}
{'rightprimer': 'GAGCCCTGGAGCCAGAAC', 'rightprimer_start': '1480', 'rightprimer_tm': '59.729472711', 'leftprimer_length': '20', 'template_length': 1477, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '24887037', 'guidecount': 25, 'leftprimer_tm': '60.0359151165', 'product_length': 1480, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'TCAATCGCCCGGTCAAATCA', 'expandedpriming': 0}
{'rightprimer': 'ACCTTATGTCTGCCCTGCAC', 'rightprimer_start': '2620', 'rightprimer_tm': '60.035055639', 'leftprimer_length': '20', 'template_length': 2636, 'leftprimer_gc': '50.0', 'leftprimer_start': '250', 'name': '25445986', 'guidecount': 25, 'leftprimer_tm': '59.8850455526', 'product_length': 2370, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AAGGGGCAATGCTTGGAAGA', 'expandedpriming': 20}
{'rightprimer': 'TGAATTAAGGGGCCCCAAGC', 'rightprimer_start': '2673', 'rightprimer_tm': '60.3252029929', 'leftprimer_length': '20', 'template_length': 2676, 'leftprimer_gc': '55.0', 'leftprimer_start': '101', 'name': '25848796', 'guidecount': 25, 'leftprimer_tm': '59.8188862673', 'product_length': 2572, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTGGTATAAGCCCTGCAGCA', 'expandedpriming': 20}
{'rightprimer': 'TCAAGAACGAGCACTTATAGAGT', 'rightprimer_start': '2107', 'rightprimer_tm': '57.1008796851', 'leftprimer_length': '25', 'template_length': 2187, 'leftprimer_gc': '40.0', 'leftprimer_start': '0', 'name': '28960563', 'guidecount': 25, 'leftprimer_tm': '57.0976128206', 'product_length': 2107, 'rightprimer_length': '23', 'rightprimer_gc': '39.1304347826', 'leftprimer': 'CATGATACCAGGTAATCTCTTGATG', 'expandedpriming': 0}
{'rightprimer': 'GCCATAACTGTGATTACAACAAGCT', 'rightprimer_start': '3470', 'rightprimer_tm': '60.1061574696', 'leftprimer_length': '20', 'template_length': 3476, 'leftprimer_gc': '55.0', 'leftprimer_start': '218', 'name': '29158458', 'guidecount': 25, 'leftprimer_tm': '59.8198287906', 'product_length': 3252, 'rightprimer_length': '25', 'rightprimer_gc': '40.0', 'leftprimer': 'TCTGCCGATACCCCATGTTG', 'expandedpriming': 20}
{'rightprimer': 'TTCCACCTGCGTCATGTTCT', 'rightprimer_start': '2188', 'rightprimer_tm': '59.6035646472', 'leftprimer_length': '20', 'template_length': 2201, 'leftprimer_gc': '55.0', 'leftprimer_start': '146', 'name': '29355927', 'guidecount': 25, 'leftprimer_tm': '59.9641192616', 'product_length': 2042, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACAGATGTGCAGGTGTGAGG', 'expandedpriming': 20}
{'rightprimer': 'GATGGTCAATCACAAGCTATGGC', 'rightprimer_start': '1661', 'rightprimer_tm': '59.9958941848', 'leftprimer_length': '20', 'template_length': 1649, 'leftprimer_gc': '55.0', 'leftprimer_start': '66', 'name': '29642150', 'guidecount': 25, 'leftprimer_tm': '60.0340888334', 'product_length': 1595, 'rightprimer_length': '23', 'rightprimer_gc': '47.8260869565', 'leftprimer': 'GTAAGGGCCTGGAGTTGCAT', 'expandedpriming': 20}
{'rightprimer': 'ACATGCAGGGCAGACACTAC', 'rightprimer_start': '2635', 'rightprimer_tm': '60.0360099994', 'leftprimer_length': '23', 'template_length': 2697, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '1', 'name': '29715763', 'guidecount': 25, 'leftprimer_tm': '60.1182234872', 'product_length': 2634, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCAGCCCCTAGTCTTATTCAAGT', 'expandedpriming': 0}
{'rightprimer': 'TCAGTCTGGGCCTATCTGGT', 'rightprimer_start': '2038', 'rightprimer_tm': '59.6601412133', 'leftprimer_length': '20', 'template_length': 2202, 'leftprimer_gc': '55.0', 'leftprimer_start': '0', 'name': '30097214', 'guidecount': 25, 'leftprimer_tm': '59.5997868814', 'product_length': 2038, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTGTAGTCCAGGCAGTTGGG', 'expandedpriming': 0}
{'rightprimer': 'TTGGGGGGTATGCCATTACG', 'rightprimer_start': '3515', 'rightprimer_tm': '59.8160291413', 'leftprimer_length': '20', 'template_length': 3524, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '30992225', 'guidecount': 25, 'leftprimer_tm': '57.7572305262', 'product_length': 3515, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGCTGCTAGTGTAAAAACGC', 'expandedpriming': 0}
{'rightprimer': 'GGCACCTAAAACAGAAGGCTC', 'rightprimer_start': '2363', 'rightprimer_tm': '59.1868221265', 'leftprimer_length': '20', 'template_length': 2375, 'leftprimer_gc': '55.0', 'leftprimer_start': '922', 'name': '31186943', 'guidecount': 25, 'leftprimer_tm': '59.2953362235', 'product_length': 1441, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'GGGGACACTCATTCCCCTTT', 'expandedpriming': 20}
{'rightprimer': 'GGGCAAGGGGGAATGACTTT', 'rightprimer_start': '916', 'rightprimer_tm': '60.2522387559', 'leftprimer_length': '20', 'template_length': 1128, 'leftprimer_gc': '55.0', 'leftprimer_start': '74', 'name': '31449253', 'guidecount': 25, 'leftprimer_tm': '60.3925947458', 'product_length': 842, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTGCTTACGTTTAGGGCCT', 'expandedpriming': 50}
{'rightprimer': 'CAACCTGAGCCTGACCCG', 'rightprimer_start': '1121', 'rightprimer_tm': '60.0468919059', 'leftprimer_length': '20', 'template_length': 1142, 'leftprimer_gc': '55.0', 'leftprimer_start': '74', 'name': '32353928', 'guidecount': 25, 'leftprimer_tm': '59.8921689425', 'product_length': 1047, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'CTGGGGGTCGTTGAACAGAA', 'expandedpriming': 50}
{'rightprimer': 'ACTTTTTGTCCTGGTATAGAACAGG', 'rightprimer_start': '2132', 'rightprimer_tm': '58.7613519997', 'leftprimer_length': '18', 'template_length': 2124, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '5', 'name': '32927696', 'guidecount': 25, 'leftprimer_tm': '58.7946050918', 'product_length': 2127, 'rightprimer_length': '25', 'rightprimer_gc': '40.0', 'leftprimer': 'CAGAGACCCGGCTCTCAG', 'expandedpriming': 20}
{'rightprimer': 'GTATCCTGCTACTAGTATACTGCT', 'rightprimer_start': '2504', 'rightprimer_tm': '56.6831722701', 'leftprimer_length': '20', 'template_length': 2487, 'leftprimer_gc': '55.0', 'leftprimer_start': '179', 'name': '36363393', 'guidecount': 25, 'leftprimer_tm': '57.5577635261', 'product_length': 2325, 'rightprimer_length': '24', 'rightprimer_gc': '41.6666666667', 'leftprimer': 'CCCTGTAGTACGAGGGGTTA', 'expandedpriming': 0}
{'rightprimer': 'GACACACTGTAACCCTTGTGC', 'rightprimer_start': '1283', 'rightprimer_tm': '59.3976232275', 'leftprimer_length': '20', 'template_length': 1288, 'leftprimer_gc': '50.0', 'leftprimer_start': '66', 'name': '37290076', 'guidecount': 25, 'leftprimer_tm': '59.5445901261', 'product_length': 1217, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'GCAGTGGTTTGTTGCTCACA', 'expandedpriming': 20}
{'rightprimer': 'GCCGCCGGAGATGGGAAA', 'rightprimer_start': '3459', 'rightprimer_tm': '62.4832432343', 'leftprimer_length': '20', 'template_length': 3444, 'leftprimer_gc': '55.0', 'leftprimer_start': '64', 'name': '37503463', 'guidecount': 25, 'leftprimer_tm': '60.0385264564', 'product_length': 3395, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'GCGGGACTTTTGAGTCGAGA', 'expandedpriming': 0}
{'rightprimer': 'GCTCATTTATATTCCGGGCGC', 'rightprimer_start': '927', 'rightprimer_tm': '59.869438763', 'leftprimer_length': '19', 'template_length': 913, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '3', 'name': '37511477', 'guidecount': 25, 'leftprimer_tm': '59.8508255471', 'product_length': 924, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'AACCTGGCAGGAGTGTGTG', 'expandedpriming': 0}
{'rightprimer': 'GATCGTTACCAGGCCACCAA', 'rightprimer_start': '1221', 'rightprimer_tm': '60.0358201114', 'leftprimer_length': '21', 'template_length': 1247, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '29', 'name': '1005690', 'guidecount': 24, 'leftprimer_tm': '60.3399201987', 'product_length': 1192, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCTCAGAATGAAATGCCGCCA', 'expandedpriming': 50}
{'rightprimer': 'CCGAAGAGCCTGCCTGATAG', 'rightprimer_start': '1688', 'rightprimer_tm': '59.9667721369', 'leftprimer_length': '20', 'template_length': 1905, 'leftprimer_gc': '55.0', 'leftprimer_start': '27', 'name': '1324812', 'guidecount': 24, 'leftprimer_tm': '59.7429605852', 'product_length': 1661, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GGGCTCTGTTGATGTGGGAT', 'expandedpriming': 0}
{'rightprimer': 'CTGCCCTCACCTCTCCAATC', 'rightprimer_start': '1247', 'rightprimer_tm': '59.8200636602', 'leftprimer_length': '20', 'template_length': 1566, 'leftprimer_gc': '55.0', 'leftprimer_start': '3', 'name': '2812082', 'guidecount': 24, 'leftprimer_tm': '59.7480844423', 'product_length': 1244, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TATCAGCCCCGGTTCTGTTG', 'expandedpriming': 0}
{'rightprimer': 'CAGAGGCCTGGAGCAGAAG', 'rightprimer_start': '2241', 'rightprimer_tm': '59.7787065969', 'leftprimer_length': '18', 'template_length': 2239, 'leftprimer_gc': '61.1111111111', 'leftprimer_start': '20', 'name': '2898615', 'guidecount': 24, 'leftprimer_tm': '59.7395661033', 'product_length': 2221, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'GACTGCAGAACGGCCGTA', 'expandedpriming': 20}
{'rightprimer': 'TCTCTGTCTTTAGGCTGTGACA', 'rightprimer_start': '2002', 'rightprimer_tm': '58.769024451', 'leftprimer_length': '20', 'template_length': 2024, 'leftprimer_gc': '55.0', 'leftprimer_start': '19', 'name': '2921551', 'guidecount': 24, 'leftprimer_tm': '59.1627275798', 'product_length': 1983, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'ACTATGTCCGGAACCCCATG', 'expandedpriming': 20}
{'rightprimer': 'TCTGTACTCATAGGCACTCCA', 'rightprimer_start': '1352', 'rightprimer_tm': '57.6179167351', 'leftprimer_length': '25', 'template_length': 1419, 'leftprimer_gc': '40.0', 'leftprimer_start': '228', 'name': '3704778', 'guidecount': 24, 'leftprimer_tm': '57.5020753977', 'product_length': 1124, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'CTTGTAGGATAGAAGTAAAATGCCC', 'expandedpriming': 0}
{'rightprimer': 'TAGAAAGCGCCACTGACCAG', 'rightprimer_start': '843', 'rightprimer_tm': '60.0373256492', 'leftprimer_length': '20', 'template_length': 1165, 'leftprimer_gc': '60.0', 'leftprimer_start': '71', 'name': '4443061', 'guidecount': 24, 'leftprimer_tm': '60.0353432429', 'product_length': 772, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCTCCACTCCTAAACCCAC', 'expandedpriming': 0}
{'rightprimer': 'GGGGGTAACTCTAGGGGGTT', 'rightprimer_start': '630', 'rightprimer_tm': '59.9546844857', 'leftprimer_length': '20', 'template_length': 1916, 'leftprimer_gc': '55.0', 'leftprimer_start': '10', 'name': '4880564', 'guidecount': 24, 'leftprimer_tm': '59.6109753153', 'product_length': 620, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CTGCTTGGGGTTGTGTTTCC', 'expandedpriming': 20}
{'rightprimer': 'GCCTCATGTGGAGCAACAGA', 'rightprimer_start': '2773', 'rightprimer_tm': '60.3219192968', 'leftprimer_length': '20', 'template_length': 2891, 'leftprimer_gc': '55.0', 'leftprimer_start': '141', 'name': '5147296', 'guidecount': 24, 'leftprimer_tm': '60.1055554548', 'product_length': 2632, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGCCCCCCATTTCATGATCC', 'expandedpriming': 0}
{'rightprimer': 'TGCAGAAATCCGGATAATTACAA', 'rightprimer_start': '1106', 'rightprimer_tm': '56.3224211948', 'leftprimer_length': '20', 'template_length': 1099, 'leftprimer_gc': '45.0', 'leftprimer_start': '4', 'name': '6637469', 'guidecount': 24, 'leftprimer_tm': '56.3585328267', 'product_length': 1102, 'rightprimer_length': '23', 'rightprimer_gc': '34.7826086957', 'leftprimer': 'TTTCCTGGTGGATCTGTTGA', 'expandedpriming': 0}
{'rightprimer': 'TCAGCCCCATAGATAGGCCA', 'rightprimer_start': '2838', 'rightprimer_tm': '59.8066785123', 'leftprimer_length': '21', 'template_length': 2862, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '10', 'name': '6703546', 'guidecount': 24, 'leftprimer_tm': '59.5271215074', 'product_length': 2828, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGAGACGTCACTGTAGCATCA', 'expandedpriming': 20}
{'rightprimer': 'GCCTCCTCCTGAATACACCG', 'rightprimer_start': '1581', 'rightprimer_tm': '59.8949608091', 'leftprimer_length': '20', 'template_length': 2073, 'leftprimer_gc': '60.0', 'leftprimer_start': '196', 'name': '6706758', 'guidecount': 24, 'leftprimer_tm': '59.8967924978', 'product_length': 1385, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GAGTCCCACGTGCCATAGAG', 'expandedpriming': 0}
{'rightprimer': 'GACTTGACTGGGTGCCATACA', 'rightprimer_start': '1960', 'rightprimer_tm': '59.9968817949', 'leftprimer_length': '20', 'template_length': 2123, 'leftprimer_gc': '55.0', 'leftprimer_start': '234', 'name': '6764388', 'guidecount': 24, 'leftprimer_tm': '60.1078483857', 'product_length': 1726, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'AATCTTACCAACCGCCTCCG', 'expandedpriming': 0}
{'rightprimer': 'TGCCTTGGTGCATTCTAGCA', 'rightprimer_start': '2808', 'rightprimer_tm': '59.961978527', 'leftprimer_length': '20', 'template_length': 2888, 'leftprimer_gc': '55.0', 'leftprimer_start': '9', 'name': '7119258', 'guidecount': 24, 'leftprimer_tm': '60.0360099994', 'product_length': 2799, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CGGTTGAGGTTCTGGCTGAT', 'expandedpriming': 20}
{'rightprimer': 'AGGCTGATGGGGTACACTCT', 'rightprimer_start': '3012', 'rightprimer_tm': '59.9566660826', 'leftprimer_length': '20', 'template_length': 3010, 'leftprimer_gc': '55.0', 'leftprimer_start': '132', 'name': '7339815', 'guidecount': 24, 'leftprimer_tm': '59.898780666', 'product_length': 2880, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGCGTTGGCTTGATTCCATC', 'expandedpriming': 0}
{'rightprimer': 'TGGTCCCTGGCAACACTG', 'rightprimer_start': '1247', 'rightprimer_tm': '59.4846015659', 'leftprimer_length': '24', 'template_length': 1243, 'leftprimer_gc': '45.8333333333', 'leftprimer_start': '9', 'name': '7410773', 'guidecount': 24, 'leftprimer_tm': '59.289625034', 'product_length': 1238, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'TGACTTCTAGGTACATCCCACTTC', 'expandedpriming': 0}
{'rightprimer': 'GTCTCCATGTTCCCTGGTGG', 'rightprimer_start': '1050', 'rightprimer_tm': '60.035055639', 'leftprimer_length': '22', 'template_length': 1064, 'leftprimer_gc': '50.0', 'leftprimer_start': '9', 'name': '8318897', 'guidecount': 24, 'leftprimer_tm': '59.9004083699', 'product_length': 1041, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TGGCCTCGTTTATAGTACCACG', 'expandedpriming': 20}
{'rightprimer': 'ACCACATTGGACATAGGTAGCTC', 'rightprimer_start': '2114', 'rightprimer_tm': '59.8651957799', 'leftprimer_length': '20', 'template_length': 2444, 'leftprimer_gc': '55.0', 'leftprimer_start': '45', 'name': '8738754', 'guidecount': 24, 'leftprimer_tm': '59.7415158117', 'product_length': 2069, 'rightprimer_length': '23', 'rightprimer_gc': '47.8260869565', 'leftprimer': 'CCCCTGATACAATGCCACCA', 'expandedpriming': 0}
{'rightprimer': 'TTCACTTTGGCCACCTACCC', 'rightprimer_start': '2067', 'rightprimer_tm': '59.8883612627', 'leftprimer_length': '20', 'template_length': 2119, 'leftprimer_gc': '55.0', 'leftprimer_start': '30', 'name': '9289233', 'guidecount': 24, 'leftprimer_tm': '59.752815348', 'product_length': 2037, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CGGGGCTACTTCGGTGTTAA', 'expandedpriming': 50}
{'rightprimer': 'GACACGGCGGCTTGTTATTC', 'rightprimer_start': '2795', 'rightprimer_tm': '59.9035457022', 'leftprimer_length': '20', 'template_length': 2812, 'leftprimer_gc': '50.0', 'leftprimer_start': '218', 'name': '9762905', 'guidecount': 24, 'leftprimer_tm': '59.9599424387', 'product_length': 2577, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTGCCATGGACCTTCTGCAT', 'expandedpriming': 0}
{'rightprimer': 'AGCATTCGCCATAGACAGCA', 'rightprimer_start': '2465', 'rightprimer_tm': '59.8212334702', 'leftprimer_length': '20', 'template_length': 3101, 'leftprimer_gc': '55.0', 'leftprimer_start': '204', 'name': '9861523', 'guidecount': 24, 'leftprimer_tm': '59.7497660004', 'product_length': 2261, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACACACTGACCGATCCCATG', 'expandedpriming': 0}
{'rightprimer': 'TCAAATACCAGTCTAGTGCCACA', 'rightprimer_start': '2287', 'rightprimer_tm': '59.4191052118', 'leftprimer_length': '18', 'template_length': 2274, 'leftprimer_gc': '61.1111111111', 'leftprimer_start': '3', 'name': '9894546', 'guidecount': 24, 'leftprimer_tm': '59.562102947', 'product_length': 2284, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'CCCAGGCAAGCAGAGGTT', 'expandedpriming': 0}
{'rightprimer': 'CAACCATGACGCTGGCAAAG', 'rightprimer_start': '2331', 'rightprimer_tm': '60.3876217414', 'leftprimer_length': '20', 'template_length': 2515, 'leftprimer_gc': '50.0', 'leftprimer_start': '292', 'name': '10189093', 'guidecount': 24, 'leftprimer_tm': '60.2512971105', 'product_length': 2039, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACAGCCCTGCAAGCAACATA', 'expandedpriming': 0}
{'rightprimer': 'TTCTAGGAAAAGAACTCGTGATTTT', 'rightprimer_start': '1996', 'rightprimer_tm': '56.9629174694', 'leftprimer_length': '20', 'template_length': 1993, 'leftprimer_gc': '50.0', 'leftprimer_start': '11', 'name': '10898358', 'guidecount': 24, 'leftprimer_tm': '57.8299879049', 'product_length': 1985, 'rightprimer_length': '25', 'rightprimer_gc': '32.0', 'leftprimer': 'TGGCCTCCCGATAGTAAAGT', 'expandedpriming': 20}
{'rightprimer': 'CCTGGGCACTGGACAAATTG', 'rightprimer_start': '1559', 'rightprimer_tm': '59.3954223282', 'leftprimer_length': '22', 'template_length': 1560, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '1', 'name': '10929938', 'guidecount': 24, 'leftprimer_tm': '59.1481787187', 'product_length': 1558, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCCCCAGGAATGATGACAATCA', 'expandedpriming': 20}
{'rightprimer': 'AACCATCTTCAGCACGCTCA', 'rightprimer_start': '4919', 'rightprimer_tm': '59.964683149', 'leftprimer_length': '20', 'template_length': 5165, 'leftprimer_gc': '55.0', 'leftprimer_start': '650', 'name': '11230796', 'guidecount': 24, 'leftprimer_tm': '59.9659413501', 'product_length': 4269, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACTCCACAGTCGGCTTCTTG', 'expandedpriming': 0}
{'rightprimer': 'TCAGCTGCAGGATTCCATGT', 'rightprimer_start': '2822', 'rightprimer_tm': '59.3778636941', 'leftprimer_length': '23', 'template_length': 3492, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '515', 'name': '11882382', 'guidecount': 24, 'leftprimer_tm': '59.4860881948', 'product_length': 2307, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGTCGGGATTCATTACTCACACT', 'expandedpriming': 0}
{'rightprimer': 'CCGCAAAACACCTGCAATGT', 'rightprimer_start': '3269', 'rightprimer_tm': '60.2494052706', 'leftprimer_length': '22', 'template_length': 3290, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '48', 'name': '13091254', 'guidecount': 24, 'leftprimer_tm': '59.8943509481', 'product_length': 3221, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGTGGGTCAAGTGTTAGATGCA', 'expandedpriming': 50}
{'rightprimer': 'GTGCTGCAGGGCTGTACTAT', 'rightprimer_start': '1591', 'rightprimer_tm': '59.8210001113', 'leftprimer_length': '22', 'template_length': 1576, 'leftprimer_gc': '50.0', 'leftprimer_start': '128', 'name': '13637602', 'guidecount': 24, 'leftprimer_tm': '59.8934890642', 'product_length': 1463, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTGGATTCCTATGGCCAGTAGC', 'expandedpriming': 0}
{'rightprimer': 'TTATTGGCTAAGGTGGGGCC', 'rightprimer_start': '2342', 'rightprimer_tm': '59.7394771289', 'leftprimer_length': '21', 'template_length': 2391, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '55', 'name': '13872568', 'guidecount': 24, 'leftprimer_tm': '59.9941946776', 'product_length': 2287, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGGCAGGCAATTCATGGTACT', 'expandedpriming': 50}
{'rightprimer': 'ATTGCTAGACCCTGGCCTAC', 'rightprimer_start': '2201', 'rightprimer_tm': '58.86750006', 'leftprimer_length': '22', 'template_length': 2377, 'leftprimer_gc': '40.9090909091', 'leftprimer_start': '24', 'name': '15257727', 'guidecount': 24, 'leftprimer_tm': '56.0715342465', 'product_length': 2177, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ATGCTAGAGAGACAATCCTCTT', 'expandedpriming': 20}
{'rightprimer': 'GGGAGAGAAGCCAGAAACCC', 'rightprimer_start': '3008', 'rightprimer_tm': '60.0349595225', 'leftprimer_length': '20', 'template_length': 2998, 'leftprimer_gc': '55.0', 'leftprimer_start': '248', 'name': '20829834', 'guidecount': 24, 'leftprimer_tm': '59.9662189864', 'product_length': 2760, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CACAATTGCCACTGGGTTGG', 'expandedpriming': 0}
{'rightprimer': 'CTGATGGTCCTGGGTGCATT', 'rightprimer_start': '2779', 'rightprimer_tm': '60.0337963284', 'leftprimer_length': '20', 'template_length': 2766, 'leftprimer_gc': '55.0', 'leftprimer_start': '180', 'name': '21553218', 'guidecount': 24, 'leftprimer_tm': '59.9653839419', 'product_length': 2599, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGCAGCAGTCAGTGTCTCTT', 'expandedpriming': 20}
{'rightprimer': 'GTGTCTTGCTAGCAACGCAT', 'rightprimer_start': '3585', 'rightprimer_tm': '59.2012243787', 'leftprimer_length': '20', 'template_length': 3588, 'leftprimer_gc': '50.0', 'leftprimer_start': '22', 'name': '21607654', 'guidecount': 24, 'leftprimer_tm': '58.6326544901', 'product_length': 3563, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGCATCCCCCAAAGTACTCA', 'expandedpriming': 20}
{'rightprimer': 'TTGGACGCCCAGCATAAAGT', 'rightprimer_start': '2216', 'rightprimer_tm': '59.9624100123', 'leftprimer_length': '20', 'template_length': 2626, 'leftprimer_gc': '55.0', 'leftprimer_start': '102', 'name': '22411079', 'guidecount': 24, 'leftprimer_tm': '59.8074339944', 'product_length': 2114, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TCCCCACACCTTAGGTCACT', 'expandedpriming': 0}
{'rightprimer': 'GGCAAAACACTACGCTTGCA', 'rightprimer_start': '1992', 'rightprimer_tm': '59.9698988443', 'leftprimer_length': '20', 'template_length': 2718, 'leftprimer_gc': '50.0', 'leftprimer_start': '810', 'name': '24212544', 'guidecount': 24, 'leftprimer_tm': '59.8883612627', 'product_length': 1182, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AAGGCGGGCTTAAAACAGGA', 'expandedpriming': 0}
{'rightprimer': 'GAATTGCCCAGGAATAGAATTCTGT', 'rightprimer_start': '1813', 'rightprimer_tm': '59.40404838', 'leftprimer_length': '20', 'template_length': 1934, 'leftprimer_gc': '60.0', 'leftprimer_start': '39', 'name': '24891970', 'guidecount': 24, 'leftprimer_tm': '60.1070169208', 'product_length': 1774, 'rightprimer_length': '25', 'rightprimer_gc': '40.0', 'leftprimer': 'CTATGACCTTGTGGCCCAGG', 'expandedpriming': 20}
{'rightprimer': 'CCCTGCGCAAAACTGAGATG', 'rightprimer_start': '1169', 'rightprimer_tm': '59.8303275341', 'leftprimer_length': '21', 'template_length': 1248, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '47', 'name': '25505840', 'guidecount': 24, 'leftprimer_tm': '59.5806133413', 'product_length': 1122, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AACTGAGAAAGTCCTGCAGCT', 'expandedpriming': 0}
{'rightprimer': 'CAACATTCCCAGGCTTCCCC', 'rightprimer_start': '645', 'rightprimer_tm': '60.9724705418', 'leftprimer_length': '20', 'template_length': 638, 'leftprimer_gc': '60.0', 'leftprimer_start': '2', 'name': '25567963', 'guidecount': 24, 'leftprimer_tm': '60.699231218', 'product_length': 643, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCCCCTCCTCCTGGCTTTAT', 'expandedpriming': 20}
{'rightprimer': 'CCGACCCAGTCCAGCTTC', 'rightprimer_start': '1625', 'rightprimer_tm': '59.7339445097', 'leftprimer_length': '20', 'template_length': 1642, 'leftprimer_gc': '55.0', 'leftprimer_start': '9', 'name': '25919457', 'guidecount': 24, 'leftprimer_tm': '59.4519689592', 'product_length': 1616, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'GAACATCTCAGGGGCATCCA', 'expandedpriming': 20}
{'rightprimer': 'AAATGGACATAGCATGACTATCCTG', 'rightprimer_start': '3046', 'rightprimer_tm': '58.763591206', 'leftprimer_length': '20', 'template_length': 3302, 'leftprimer_gc': '55.0', 'leftprimer_start': '66', 'name': '26023731', 'guidecount': 24, 'leftprimer_tm': '59.3069514685', 'product_length': 2980, 'rightprimer_length': '25', 'rightprimer_gc': '40.0', 'leftprimer': 'GTCTGACCCTGGGTTGACAT', 'expandedpriming': 50}
{'rightprimer': 'GGAACCTTCCCTGGCTTTAGT', 'rightprimer_start': '1775', 'rightprimer_tm': '59.6452949692', 'leftprimer_length': '20', 'template_length': 1767, 'leftprimer_gc': '55.0', 'leftprimer_start': '2', 'name': '26035237', 'guidecount': 24, 'leftprimer_tm': '59.6106092841', 'product_length': 1773, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'ACTTCACCGGTGTCAGTGAG', 'expandedpriming': 0}
{'rightprimer': 'ACCACAGGAACTCACCACAG', 'rightprimer_start': '2465', 'rightprimer_tm': '59.5300828439', 'leftprimer_length': '20', 'template_length': 2482, 'leftprimer_gc': '55.0', 'leftprimer_start': '10', 'name': '27451707', 'guidecount': 24, 'leftprimer_tm': '60.1070169208', 'product_length': 2455, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGAGCCCTTTAGCATGAGCA', 'expandedpriming': 20}
{'rightprimer': 'GCTGGGCCTTGCAGGTAATA', 'rightprimer_start': '3438', 'rightprimer_tm': '60.1067190722', 'leftprimer_length': '20', 'template_length': 3523, 'leftprimer_gc': '50.0', 'leftprimer_start': '250', 'name': '28186520', 'guidecount': 24, 'leftprimer_tm': '60.1062679146', 'product_length': 3188, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGTTGTTGACCCATGCTGGT', 'expandedpriming': 0}
{'rightprimer': 'GGAGTGGGGTTCCTGGGA', 'rightprimer_start': '2081', 'rightprimer_tm': '60.2040896537', 'leftprimer_length': '21', 'template_length': 2078, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '15', 'name': '28379011', 'guidecount': 24, 'leftprimer_tm': '60.202818324', 'product_length': 2066, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'GGCTTTCAGTTCAGTGACCCT', 'expandedpriming': 20}
{'rightprimer': 'AATTCTGACCATGTGGCAACTC', 'rightprimer_start': '1545', 'rightprimer_tm': '59.1780309037', 'leftprimer_length': '21', 'template_length': 1554, 'leftprimer_gc': '42.8571428571', 'leftprimer_start': '23', 'name': '28561294', 'guidecount': 24, 'leftprimer_tm': '59.380667953', 'product_length': 1522, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'TGCTGGCAATCAATTCAAGCA', 'expandedpriming': 20}
{'rightprimer': 'TTGGTGCTACCCGTGTTACC', 'rightprimer_start': '2441', 'rightprimer_tm': '59.9648236679', 'leftprimer_length': '20', 'template_length': 3105, 'leftprimer_gc': '55.0', 'leftprimer_start': '407', 'name': '28768132', 'guidecount': 24, 'leftprimer_tm': '59.8303275341', 'product_length': 2034, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAAATGCGCTCTCCACCTTG', 'expandedpriming': 0}
{'rightprimer': 'GCTGTCTAGTCTGAGAGCGG', 'rightprimer_start': '1685', 'rightprimer_tm': '59.6196218235', 'leftprimer_length': '21', 'template_length': 1682, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '4', 'name': '30362584', 'guidecount': 24, 'leftprimer_tm': '59.9255948985', 'product_length': 1681, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCAGGGGGCATCTTCATATCA', 'expandedpriming': 20}
{'rightprimer': 'CCACATTGTCCTTCCTCCCC', 'rightprimer_start': '2624', 'rightprimer_tm': '60.0340888334', 'leftprimer_length': '20', 'template_length': 4003, 'leftprimer_gc': '55.0', 'leftprimer_start': '458', 'name': '30759399', 'guidecount': 24, 'leftprimer_tm': '60.0346704238', 'product_length': 2166, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'ACAGATTCCTTCAGCTGCCC', 'expandedpriming': 0}
{'rightprimer': 'CAGAAAGCATTGACCTCGGGA', 'rightprimer_start': '2054', 'rightprimer_tm': '60.3390552047', 'leftprimer_length': '20', 'template_length': 2077, 'leftprimer_gc': '55.0', 'leftprimer_start': '154', 'name': '30866518', 'guidecount': 24, 'leftprimer_tm': '60.0341860809', 'product_length': 1900, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'TTGGATTAGCCCTGTGCCTG', 'expandedpriming': 0}
{'rightprimer': 'AGGAGTAAACGGCAATCGGT', 'rightprimer_start': '2650', 'rightprimer_tm': '59.3882858534', 'leftprimer_length': '20', 'template_length': 2643, 'leftprimer_gc': '50.0', 'leftprimer_start': '856', 'name': '30970922', 'guidecount': 24, 'leftprimer_tm': '59.318824876', 'product_length': 1794, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AGCATGGACAAAACTTGCCC', 'expandedpriming': 20}
{'rightprimer': 'ACTGCATTCATGGGAGGGTG', 'rightprimer_start': '2527', 'rightprimer_tm': '60.0337963284', 'leftprimer_length': '20', 'template_length': 2600, 'leftprimer_gc': '60.0', 'leftprimer_start': '265', 'name': '31877494', 'guidecount': 24, 'leftprimer_tm': '59.9616899326', 'product_length': 2262, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAGGGGTTCCTTGGAAGACC', 'expandedpriming': 0}
{'rightprimer': 'TCCCCTTTGAGTACAGTGTGC', 'rightprimer_start': '2325', 'rightprimer_tm': '59.9283008324', 'leftprimer_length': '20', 'template_length': 2312, 'leftprimer_gc': '50.0', 'leftprimer_start': '92', 'name': '33292716', 'guidecount': 24, 'leftprimer_tm': '59.963977835', 'product_length': 2233, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'TGATTGACATCACGTGGGCA', 'expandedpriming': 0}
{'rightprimer': 'GCAGTTGTCAGATGGTGGGA', 'rightprimer_start': '1433', 'rightprimer_tm': '59.9632679558', 'leftprimer_length': '24', 'template_length': 2149, 'leftprimer_gc': '45.8333333333', 'leftprimer_start': '176', 'name': '33360625', 'guidecount': 24, 'leftprimer_tm': '58.5375945204', 'product_length': 1257, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAGTGACACTTCAGGTAGTAGAAC', 'expandedpriming': 0}
{'rightprimer': 'GGGGGCGTCTAATAACGTGT', 'rightprimer_start': '2275', 'rightprimer_tm': '59.8235506325', 'leftprimer_length': '20', 'template_length': 2356, 'leftprimer_gc': '55.0', 'leftprimer_start': '140', 'name': '33427769', 'guidecount': 24, 'leftprimer_tm': '59.6618801403', 'product_length': 2135, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGGAATTGTGGGGATTGGGA', 'expandedpriming': 0}
{'rightprimer': 'TTCAACGACTGGAAAGAGCCA', 'rightprimer_start': '1724', 'rightprimer_tm': '59.8599477734', 'leftprimer_length': '20', 'template_length': 1728, 'leftprimer_gc': '55.0', 'leftprimer_start': '118', 'name': '33809631', 'guidecount': 24, 'leftprimer_tm': '59.8242400093', 'product_length': 1606, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'TGTGAAGCCGCTCAGGATAC', 'expandedpriming': 0}
{'rightprimer': 'TGACATCTTCCGTACCTCCCT', 'rightprimer_start': '1688', 'rightprimer_tm': '59.9932417813', 'leftprimer_length': '20', 'template_length': 1788, 'leftprimer_gc': '55.0', 'leftprimer_start': '41', 'name': '35263234', 'guidecount': 24, 'leftprimer_tm': '60.2508576952', 'product_length': 1647, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'CTGGCAACACCTCATGGACA', 'expandedpriming': 0}
{'rightprimer': 'TGCCTGCTACATTCATTCTCA', 'rightprimer_start': '1335', 'rightprimer_tm': '57.3673567928', 'leftprimer_length': '23', 'template_length': 1655, 'leftprimer_gc': '39.1304347826', 'leftprimer_start': '536', 'name': '37273646', 'guidecount': 24, 'leftprimer_tm': '57.3012592401', 'product_length': 799, 'rightprimer_length': '21', 'rightprimer_gc': '42.8571428571', 'leftprimer': 'TCCCTCACAATAACATGAGATGT', 'expandedpriming': 0}
{'rightprimer': 'CCACACCAAAGGTCAAAGCG', 'rightprimer_start': '1567', 'rightprimer_tm': '59.9693615711', 'leftprimer_length': '21', 'template_length': 1561, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '3', 'name': '85280', 'guidecount': 23, 'leftprimer_tm': '60.0644628227', 'product_length': 1564, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCTGGCCCATAAAGAAGAGCA', 'expandedpriming': 20}
{'rightprimer': 'ACCTTTGAACCCCATAGAGC', 'rightprimer_start': '1271', 'rightprimer_tm': '57.1910938873', 'leftprimer_length': '20', 'template_length': 1283, 'leftprimer_gc': '50.0', 'leftprimer_start': '11', 'name': '941923', 'guidecount': 23, 'leftprimer_tm': '59.3794444032', 'product_length': 1260, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AACTGGGATCCATGCTCGTT', 'expandedpriming': 20}
{'rightprimer': 'TGCGCTGTGTGTTGAGTAGT', 'rightprimer_start': '1622', 'rightprimer_tm': '59.8958795713', 'leftprimer_length': '21', 'template_length': 1657, 'leftprimer_gc': '57.1428571429', 'leftprimer_start': '7', 'name': '1096465', 'guidecount': 23, 'leftprimer_tm': '59.7335536878', 'product_length': 1615, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GCCGGATCTACTAACGAGTCC', 'expandedpriming': 0}
{'rightprimer': 'GGTGCTTCCAGGTTTCCTCT', 'rightprimer_start': '932', 'rightprimer_tm': '59.5978794762', 'leftprimer_length': '20', 'template_length': 915, 'leftprimer_gc': '55.0', 'leftprimer_start': '22', 'name': '1271520', 'guidecount': 23, 'leftprimer_tm': '59.9603818904', 'product_length': 910, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ATGGGTACAGCTGTTTGGGG', 'expandedpriming': 0}
{'rightprimer': 'ATGAGCCTGAGGGACAAACT', 'rightprimer_start': '1054', 'rightprimer_tm': '58.6336814162', 'leftprimer_length': '20', 'template_length': 1115, 'leftprimer_gc': '50.0', 'leftprimer_start': '64', 'name': '1470134', 'guidecount': 23, 'leftprimer_tm': '58.5868822461', 'product_length': 990, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGTGCTCAGGTCTCAAGTGA', 'expandedpriming': 50}
{'rightprimer': 'AAGGTGGAGCCCATCATCCA', 'rightprimer_start': '1807', 'rightprimer_tm': '60.9207632938', 'leftprimer_length': '18', 'template_length': 2411, 'leftprimer_gc': '72.2222222222', 'leftprimer_start': '16', 'name': '1512746', 'guidecount': 23, 'leftprimer_tm': '61.1510220606', 'product_length': 1791, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCGGCTCTCTGACCCCTC', 'expandedpriming': 0}
{'rightprimer': 'GGGACCCTGGTAATAACACCTG', 'rightprimer_start': '2255', 'rightprimer_tm': '60.0925916332', 'leftprimer_length': '20', 'template_length': 2261, 'leftprimer_gc': '55.0', 'leftprimer_start': '110', 'name': '1799204', 'guidecount': 23, 'leftprimer_tm': '60.1789924141', 'product_length': 2145, 'rightprimer_length': '22', 'rightprimer_gc': '54.5454545455', 'leftprimer': 'TGGAACCCCAGTCAAGCTTG', 'expandedpriming': 20}
{'rightprimer': 'CGGGGAGCGTAGACTTGG', 'rightprimer_start': '1729', 'rightprimer_tm': '59.505082', 'leftprimer_length': '20', 'template_length': 1732, 'leftprimer_gc': '55.0', 'leftprimer_start': '180', 'name': '2522019', 'guidecount': 23, 'leftprimer_tm': '60.3246449465', 'product_length': 1549, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'GGGGAATGTGCCACCATCTT', 'expandedpriming': 20}
{'rightprimer': 'GCTCATAATGAAACCCCAGGATTC', 'rightprimer_start': '1409', 'rightprimer_tm': '59.720579935', 'leftprimer_length': '20', 'template_length': 1393, 'leftprimer_gc': '55.0', 'leftprimer_start': '387', 'name': '2734273', 'guidecount': 23, 'leftprimer_tm': '59.3545039413', 'product_length': 1022, 'rightprimer_length': '24', 'rightprimer_gc': '45.8333333333', 'leftprimer': 'TCTCTACCCCATAAGGCCCA', 'expandedpriming': 0}
{'rightprimer': 'ACCACACCTTTCTTGCAGGT', 'rightprimer_start': '1131', 'rightprimer_tm': '59.7412257187', 'leftprimer_length': '20', 'template_length': 1326, 'leftprimer_gc': '60.0', 'leftprimer_start': '24', 'name': '2896868', 'guidecount': 23, 'leftprimer_tm': '59.7308268219', 'product_length': 1107, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CCCCTCTTAGGTCCCCTCAT', 'expandedpriming': 20}
{'rightprimer': 'TAAGCTAAGCACTGGGCCTG', 'rightprimer_start': '2352', 'rightprimer_tm': '59.7469561384', 'leftprimer_length': '19', 'template_length': 2423, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '73', 'name': '3038836', 'guidecount': 23, 'leftprimer_tm': '59.4919123706', 'product_length': 2279, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CGAAGGCCAAATGACCTGC', 'expandedpriming': 50}
{'rightprimer': 'TCCACTAGGAATGTGCCAATAATGT', 'rightprimer_start': '1236', 'rightprimer_tm': '60.3399439698', 'leftprimer_length': '20', 'template_length': 1234, 'leftprimer_gc': '50.0', 'leftprimer_start': '280', 'name': '3100546', 'guidecount': 23, 'leftprimer_tm': '60.035055639', 'product_length': 956, 'rightprimer_length': '25', 'rightprimer_gc': '40.0', 'leftprimer': 'ACCCGCTGATTGCTAGCTTT', 'expandedpriming': 20}
{'rightprimer': 'CGGAGCAGGTTTGTCAGGAT', 'rightprimer_start': '2019', 'rightprimer_tm': '60.0360099994', 'leftprimer_length': '20', 'template_length': 2005, 'leftprimer_gc': '55.0', 'leftprimer_start': '992', 'name': '3494264', 'guidecount': 23, 'leftprimer_tm': '60.0365767414', 'product_length': 1027, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGCAGCTTGGCACTGTCTAG', 'expandedpriming': 20}
{'rightprimer': 'TGCACTGCCCGTTGTTATCT', 'rightprimer_start': '5742', 'rightprimer_tm': '59.9638362258', 'leftprimer_length': '20', 'template_length': 5737, 'leftprimer_gc': '55.0', 'leftprimer_start': '56', 'name': '4043680', 'guidecount': 23, 'leftprimer_tm': '60.1065192116', 'product_length': 5686, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ATGTCAGCCATAACCCAGGC', 'expandedpriming': 20}
{'rightprimer': 'CAGCCATAATAGGAGCACGGT', 'rightprimer_start': '2620', 'rightprimer_tm': '59.9292423525', 'leftprimer_length': '22', 'template_length': 2647, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '801', 'name': '4095492', 'guidecount': 23, 'leftprimer_tm': '59.9597219144', 'product_length': 1819, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'TCTCCCAGCAATACTTTGTGCT', 'expandedpriming': 50}
{'rightprimer': 'ATGGCTGGTACATGGCTGTC', 'rightprimer_start': '844', 'rightprimer_tm': '60.1070169208', 'leftprimer_length': '21', 'template_length': 901, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '47', 'name': '4269325', 'guidecount': 23, 'leftprimer_tm': '60.2705181208', 'product_length': 797, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACTGCATTGCTTCTCGTGTCT', 'expandedpriming': 50}
{'rightprimer': 'ACCCACATTATCACTAAAAGCC', 'rightprimer_start': '2469', 'rightprimer_tm': '56.393601403', 'leftprimer_length': '20', 'template_length': 2479, 'leftprimer_gc': '55.0', 'leftprimer_start': '396', 'name': '4547193', 'guidecount': 23, 'leftprimer_tm': '59.4004418713', 'product_length': 2073, 'rightprimer_length': '22', 'rightprimer_gc': '40.9090909091', 'leftprimer': 'TTTCTACGTCACGTCCCTGG', 'expandedpriming': 20}
{'rightprimer': 'TAAAGGGGTACTGTCGTGCC', 'rightprimer_start': '1897', 'rightprimer_tm': '59.3903380036', 'leftprimer_length': '22', 'template_length': 2498, 'leftprimer_gc': '50.0', 'leftprimer_start': '6', 'name': '4590167', 'guidecount': 23, 'leftprimer_tm': '59.357192839', 'product_length': 1891, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTTTTTCTAGTCCCCACCTCCA', 'expandedpriming': 0}
{'rightprimer': 'TTTTTGTTGGTAAGTGCCGGA', 'rightprimer_start': '1259', 'rightprimer_tm': '58.6238912292', 'leftprimer_length': '20', 'template_length': 1284, 'leftprimer_gc': '55.0', 'leftprimer_start': '27', 'name': '4694458', 'guidecount': 23, 'leftprimer_tm': '58.8828498278', 'product_length': 1232, 'rightprimer_length': '21', 'rightprimer_gc': '42.8571428571', 'leftprimer': 'TACTCCGATGCTAACCCCAC', 'expandedpriming': 20}
{'rightprimer': 'ATACCCAGGTGGCAGAGGT', 'rightprimer_start': '1546', 'rightprimer_tm': '59.9170463021', 'leftprimer_length': '20', 'template_length': 2093, 'leftprimer_gc': '55.0', 'leftprimer_start': '24', 'name': '5542893', 'guidecount': 23, 'leftprimer_tm': '59.1161666332', 'product_length': 1522, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'CCACAAGGCAGCTAAAGGTG', 'expandedpriming': 20}
{'rightprimer': 'TACTGACCCGGCACATGTG', 'rightprimer_start': '2465', 'rightprimer_tm': '59.7048986421', 'leftprimer_length': '20', 'template_length': 2463, 'leftprimer_gc': '60.0', 'leftprimer_start': '0', 'name': '5567574', 'guidecount': 23, 'leftprimer_tm': '59.8912262683', 'product_length': 2465, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'GGCTGCCCCTAGTATGGAAC', 'expandedpriming': 0}
{'rightprimer': 'GGCCTTCCTAACTAGTCACTCAC', 'rightprimer_start': '2340', 'rightprimer_tm': '60.1190646319', 'leftprimer_length': '20', 'template_length': 2362, 'leftprimer_gc': '60.0', 'leftprimer_start': '9', 'name': '5851766', 'guidecount': 23, 'leftprimer_tm': '60.3269995853', 'product_length': 2331, 'rightprimer_length': '23', 'rightprimer_gc': '52.1739130435', 'leftprimer': 'AGGGGATGGAGGAGTGAAGG', 'expandedpriming': 20}
{'rightprimer': 'ATCAGAGTGTGGGTCTCCCT', 'rightprimer_start': '1000', 'rightprimer_tm': '59.5845709194', 'leftprimer_length': '20', 'template_length': 1240, 'leftprimer_gc': '55.0', 'leftprimer_start': '5', 'name': '6692766', 'guidecount': 23, 'leftprimer_tm': '59.4678849418', 'product_length': 995, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CACCAGGCCAACAATTAGGC', 'expandedpriming': 0}
{'rightprimer': 'CAGGCAGGAACCTTAAAAAGAGA', 'rightprimer_start': '1694', 'rightprimer_tm': '58.6016814013', 'leftprimer_length': '20', 'template_length': 1696, 'leftprimer_gc': '55.0', 'leftprimer_start': '50', 'name': '6694419', 'guidecount': 23, 'leftprimer_tm': '58.641848117', 'product_length': 1644, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'GGTTCCCATGATATCCGGGA', 'expandedpriming': 20}
{'rightprimer': 'CCTTGTTTTGCTAGATTAACAGAC', 'rightprimer_start': '1879', 'rightprimer_tm': '56.2822684327', 'leftprimer_length': '20', 'template_length': 1871, 'leftprimer_gc': '45.0', 'leftprimer_start': '182', 'name': '6771884', 'guidecount': 23, 'leftprimer_tm': '56.4533766688', 'product_length': 1697, 'rightprimer_length': '24', 'rightprimer_gc': '37.5', 'leftprimer': 'TGGTGCCATACTTCTTCACT', 'expandedpriming': 0}
{'rightprimer': 'AGCTCTCCGGCTTGCAATT', 'rightprimer_start': '1964', 'rightprimer_tm': '60.0016405343', 'leftprimer_length': '20', 'template_length': 1960, 'leftprimer_gc': '50.0', 'leftprimer_start': '46', 'name': '8445121', 'guidecount': 23, 'leftprimer_tm': '60.0326147522', 'product_length': 1918, 'rightprimer_length': '19', 'rightprimer_gc': '52.6315789474', 'leftprimer': 'TTTGGGACTTGTGGCTGGTT', 'expandedpriming': 0}
{'rightprimer': 'ATCCTTCACAAATTCATCCTTCA', 'rightprimer_start': '884', 'rightprimer_tm': '56.1491920957', 'leftprimer_length': '20', 'template_length': 970, 'leftprimer_gc': '50.0', 'leftprimer_start': '67', 'name': '8955297', 'guidecount': 23, 'leftprimer_tm': '56.193610047', 'product_length': 817, 'rightprimer_length': '23', 'rightprimer_gc': '34.7826086957', 'leftprimer': 'AGGGTAGGAGACATATCCCA', 'expandedpriming': 50}
{'rightprimer': 'GCTGCAGAGAGATGGATGCT', 'rightprimer_start': '1704', 'rightprimer_tm': '59.8929187124', 'leftprimer_length': '20', 'template_length': 2610, 'leftprimer_gc': '50.0', 'leftprimer_start': '62', 'name': '9850430', 'guidecount': 23, 'leftprimer_tm': '59.8932921511', 'product_length': 1642, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCTTGGCGTCTCAGCTTTGT', 'expandedpriming': 50}
{'rightprimer': 'ACTTTCTGGGACATCAATCTCTT', 'rightprimer_start': '973', 'rightprimer_tm': '57.4886136838', 'leftprimer_length': '20', 'template_length': 995, 'leftprimer_gc': '55.0', 'leftprimer_start': '6', 'name': '10159304', 'guidecount': 23, 'leftprimer_tm': '58.5583052688', 'product_length': 967, 'rightprimer_length': '23', 'rightprimer_gc': '39.1304347826', 'leftprimer': 'CCCCGGGTTCCAATACCTAT', 'expandedpriming': 20}
{'rightprimer': 'TTGCAATTGTGTGGGTCGAC', 'rightprimer_start': '1601', 'rightprimer_tm': '59.3341645019', 'leftprimer_length': '21', 'template_length': 1615, 'leftprimer_gc': '57.1428571429', 'leftprimer_start': '8', 'name': '10638849', 'guidecount': 23, 'leftprimer_tm': '58.9521698634', 'product_length': 1593, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CCCAGGGTAACTATCACTCCC', 'expandedpriming': 20}
{'rightprimer': 'GGCTAGATGCTGACCACAATGA', 'rightprimer_start': '3478', 'rightprimer_tm': '60.4205474496', 'leftprimer_length': '20', 'template_length': 3461, 'leftprimer_gc': '55.0', 'leftprimer_start': '372', 'name': '11209543', 'guidecount': 23, 'leftprimer_tm': '60.0367646877', 'product_length': 3106, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'TGCCGGTGTTACAGTGGATC', 'expandedpriming': 20}
{'rightprimer': 'ATCTAGCCCTCTGTTGTTTGT', 'rightprimer_start': '2256', 'rightprimer_tm': '56.9219441933', 'leftprimer_length': '21', 'template_length': 2256, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '113', 'name': '11272305', 'guidecount': 23, 'leftprimer_tm': '57.251355022', 'product_length': 2143, 'rightprimer_length': '21', 'rightprimer_gc': '42.8571428571', 'leftprimer': 'TCTCCTAGAGAAGAAGCCAGT', 'expandedpriming': 20}
{'rightprimer': 'AAGGACAATGTGGCACCTGG', 'rightprimer_start': '675', 'rightprimer_tm': '60.5403647704', 'leftprimer_length': '20', 'template_length': 738, 'leftprimer_gc': '55.0', 'leftprimer_start': '178', 'name': '11474459', 'guidecount': 23, 'leftprimer_tm': '60.5342025765', 'product_length': 497, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGATTAAATCCGCCGGCAGA', 'expandedpriming': 50}
{'rightprimer': 'ATTTTCCGGGGACTTGCTGT', 'rightprimer_start': '3204', 'rightprimer_tm': '59.8875877447', 'leftprimer_length': '20', 'template_length': 3201, 'leftprimer_gc': '55.0', 'leftprimer_start': '287', 'name': '11650697', 'guidecount': 23, 'leftprimer_tm': '60.0371391397', 'product_length': 2917, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CTCAGCAGCAAGACAGCTCT', 'expandedpriming': 20}
{'rightprimer': 'GGGGTGCTAACTCCCTTGATC', 'rightprimer_start': '1834', 'rightprimer_tm': '60.1340675918', 'leftprimer_length': '20', 'template_length': 1879, 'leftprimer_gc': '60.0', 'leftprimer_start': '848', 'name': '13218156', 'guidecount': 23, 'leftprimer_tm': '60.1052460662', 'product_length': 986, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'CCCCTAGATGCCAGGAGAGT', 'expandedpriming': 50}
{'rightprimer': 'TGGCTGCTCTGTGGATAAGC', 'rightprimer_start': '1669', 'rightprimer_tm': '60.1075082218', 'leftprimer_length': '22', 'template_length': 2345, 'leftprimer_gc': '50.0', 'leftprimer_start': '3', 'name': '13667153', 'guidecount': 23, 'leftprimer_tm': '60.1587401168', 'product_length': 1666, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGCTCCTAGAGTCTGCGATACT', 'expandedpriming': 0}
{'rightprimer': 'GGACCGCCAGCTGTACATTT', 'rightprimer_start': '1180', 'rightprimer_tm': '60.6767820242', 'leftprimer_length': '20', 'template_length': 1218, 'leftprimer_gc': '55.0', 'leftprimer_start': '75', 'name': '14978523', 'guidecount': 23, 'leftprimer_tm': '60.6055893377', 'product_length': 1105, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGGCCTTTACTGCTCGTTGT', 'expandedpriming': 50}
{'rightprimer': 'AAAATATGACCGGGGGCCC', 'rightprimer_start': '2252', 'rightprimer_tm': '59.7680717901', 'leftprimer_length': '20', 'template_length': 2245, 'leftprimer_gc': '60.0', 'leftprimer_start': '119', 'name': '15369050', 'guidecount': 23, 'leftprimer_tm': '60.1070169208', 'product_length': 2133, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'CCTGTGTCTATGGGGCCAAG', 'expandedpriming': 20}
{'rightprimer': 'GGAGCTGCGAGGGTAATAGA', 'rightprimer_start': '1981', 'rightprimer_tm': '58.6707443632', 'leftprimer_length': '20', 'template_length': 2131, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '15402013', 'guidecount': 23, 'leftprimer_tm': '57.8754396671', 'product_length': 1981, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ATATACCCCAGGAGCAGCTT', 'expandedpriming': 0}
{'rightprimer': 'ATGACACGTGGGCAATCACT', 'rightprimer_start': '1696', 'rightprimer_tm': '59.9632679558', 'leftprimer_length': '20', 'template_length': 1764, 'leftprimer_gc': '60.0', 'leftprimer_start': '3', 'name': '15416621', 'guidecount': 23, 'leftprimer_tm': '59.8912262683', 'product_length': 1693, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGTGCTAATCCCTAGGCACC', 'expandedpriming': 0}
{'rightprimer': 'ATTTAACAAGCGCTGGGGGA', 'rightprimer_start': '1657', 'rightprimer_tm': '59.9609651436', 'leftprimer_length': '20', 'template_length': 1802, 'leftprimer_gc': '55.0', 'leftprimer_start': '0', 'name': '15600286', 'guidecount': 23, 'leftprimer_tm': '59.5821592885', 'product_length': 1657, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AGGGACACCAGGGTAGTGAT', 'expandedpriming': 0}
{'rightprimer': 'GCCGCCAACTGGTGTTTTAA', 'rightprimer_start': '3381', 'rightprimer_tm': '59.6138868069', 'leftprimer_length': '21', 'template_length': 3916, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '4', 'name': '15639287', 'guidecount': 23, 'leftprimer_tm': '59.5206771551', 'product_length': 3377, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACAGCCGGCGATACTAAAAGA', 'expandedpriming': 0}
{'rightprimer': 'TCCTGACCGGCATTTCTAAC', 'rightprimer_start': '2133', 'rightprimer_tm': '57.6034103202', 'leftprimer_length': '21', 'template_length': 2129, 'leftprimer_gc': '42.8571428571', 'leftprimer_start': '205', 'name': '16439189', 'guidecount': 23, 'leftprimer_tm': '57.2117728825', 'product_length': 1928, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TTGAGCCTGTAACAATGTCCT', 'expandedpriming': 0}
{'rightprimer': 'ACTAGTAGCGTCAAAGCATCCA', 'rightprimer_start': '2617', 'rightprimer_tm': '59.5039304164', 'leftprimer_length': '20', 'template_length': 2618, 'leftprimer_gc': '55.0', 'leftprimer_start': '1405', 'name': '16954839', 'guidecount': 23, 'leftprimer_tm': '59.5521988839', 'product_length': 1212, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'CCGTTACAGCAAGCCATGTC', 'expandedpriming': 20}
{'rightprimer': 'TAAACCACAGAATGCACGCC', 'rightprimer_start': '577', 'rightprimer_tm': '59.1215836542', 'leftprimer_length': '19', 'template_length': 583, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '17', 'name': '17449696', 'guidecount': 23, 'leftprimer_tm': '59.8471702794', 'product_length': 560, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ATCTCTCGGCCCCTCTCTC', 'expandedpriming': 20}
{'rightprimer': 'GCTAGGCAGGCATCAGCATA', 'rightprimer_start': '2033', 'rightprimer_tm': '59.9641192616', 'leftprimer_length': '20', 'template_length': 2043, 'leftprimer_gc': '55.0', 'leftprimer_start': '6', 'name': '17675081', 'guidecount': 23, 'leftprimer_tm': '60.538746475', 'product_length': 2027, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCGGGAAAAAAGGTCAGGA', 'expandedpriming': 20}
{'rightprimer': 'TCTGAAACTGCAACGGTCCA', 'rightprimer_start': '2142', 'rightprimer_tm': '59.8207664515', 'leftprimer_length': '23', 'template_length': 2153, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '3', 'name': '17958168', 'guidecount': 23, 'leftprimer_tm': '59.7220641778', 'product_length': 2139, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AAGGACTAGGGGTTAGGATTCCT', 'expandedpriming': 0}
{'rightprimer': 'CACGGTGTGTAGGAGGTGAT', 'rightprimer_start': '1761', 'rightprimer_tm': '59.1044699215', 'leftprimer_length': '24', 'template_length': 2167, 'leftprimer_gc': '37.5', 'leftprimer_start': '62', 'name': '20004522', 'guidecount': 23, 'leftprimer_tm': '58.4858311266', 'product_length': 1699, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTTGGCATTATTGTCCTATGGTCA', 'expandedpriming': 50}
{'rightprimer': 'CCATGTGAGGCGACAGGTAA', 'rightprimer_start': '2473', 'rightprimer_tm': '59.7508798392', 'leftprimer_length': '19', 'template_length': 2527, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '14', 'name': '20779280', 'guidecount': 23, 'leftprimer_tm': '59.852846915', 'product_length': 2459, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTCCGGAGCTTGGGGATTC', 'expandedpriming': 20}
{'rightprimer': 'TGGTTTGTCACTGTACTGCCT', 'rightprimer_start': '1047', 'rightprimer_tm': '59.5111884718', 'leftprimer_length': '20', 'template_length': 1183, 'leftprimer_gc': '55.0', 'leftprimer_start': '55', 'name': '21723569', 'guidecount': 23, 'leftprimer_tm': '59.8049038557', 'product_length': 992, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'TGGTCCCAGGTACCTTTCCT', 'expandedpriming': 50}
{'rightprimer': 'GGAGGATAAGCATGGGCCAA', 'rightprimer_start': '2512', 'rightprimer_tm': '59.8140993723', 'leftprimer_length': '20', 'template_length': 2501, 'leftprimer_gc': '55.0', 'leftprimer_start': '489', 'name': '21909718', 'guidecount': 23, 'leftprimer_tm': '59.9656630029', 'product_length': 2023, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCGGTCTGTGTCTTGGGTAA', 'expandedpriming': 20}
{'rightprimer': 'TGGATCTTCCAGGCTGGA', 'rightprimer_start': '715', 'rightprimer_tm': '57.0825244826', 'leftprimer_length': '20', 'template_length': 712, 'leftprimer_gc': '55.0', 'leftprimer_start': '20', 'name': '26864673', 'guidecount': 23, 'leftprimer_tm': '59.8174632821', 'product_length': 695, 'rightprimer_length': '18', 'rightprimer_gc': '55.5555555556', 'leftprimer': 'TGGTGTCTTTGGACCCACTG', 'expandedpriming': 20}
{'rightprimer': 'ACTCACTACACTCAAGTACAGCA', 'rightprimer_start': '1461', 'rightprimer_tm': '59.1104619616', 'leftprimer_length': '20', 'template_length': 1452, 'leftprimer_gc': '55.0', 'leftprimer_start': '513', 'name': '27609340', 'guidecount': 23, 'leftprimer_tm': '59.8887465063', 'product_length': 948, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'CCAGGCCAAGTCAAAGACCT', 'expandedpriming': 20}
{'rightprimer': 'GGACCTGGGAGATCTATTTGT', 'rightprimer_start': '4112', 'rightprimer_tm': '56.4937984879', 'leftprimer_length': '20', 'template_length': 4095, 'leftprimer_gc': '55.0', 'leftprimer_start': '454', 'name': '28404474', 'guidecount': 23, 'leftprimer_tm': '58.2435699443', 'product_length': 3658, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'GAGGTGCAAAAGATCCCTCC', 'expandedpriming': 20}
{'rightprimer': 'GGGTTACCACCTGTTCCATCT', 'rightprimer_start': '2586', 'rightprimer_tm': '59.366054848', 'leftprimer_length': '20', 'template_length': 2578, 'leftprimer_gc': '50.0', 'leftprimer_start': '24', 'name': '28764065', 'guidecount': 23, 'leftprimer_tm': '59.3836294537', 'product_length': 2562, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'ACACCTAATGCGGCACCTAA', 'expandedpriming': 20}
{'rightprimer': 'TTGGGTTTCCACCTCACCAC', 'rightprimer_start': '3383', 'rightprimer_tm': '60.1064188897', 'leftprimer_length': '20', 'template_length': 3390, 'leftprimer_gc': '55.0', 'leftprimer_start': '32', 'name': '28790001', 'guidecount': 23, 'leftprimer_tm': '60.1789725539', 'product_length': 3351, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGTTGGTGCTTGGGGTTACT', 'expandedpriming': 20}
{'rightprimer': 'TGGCAAACATCAGACCCACA', 'rightprimer_start': '2291', 'rightprimer_tm': '59.8153078464', 'leftprimer_length': '20', 'template_length': 2337, 'leftprimer_gc': '55.0', 'leftprimer_start': '68', 'name': '28983085', 'guidecount': 23, 'leftprimer_tm': '59.6757148927', 'product_length': 2223, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CCTGCTGTTCTTCTGAGGCT', 'expandedpriming': 50}
{'rightprimer': 'GGATAACCATTCCATAGGTGGCA', 'rightprimer_start': '1522', 'rightprimer_tm': '60.1814336666', 'leftprimer_length': '20', 'template_length': 1562, 'leftprimer_gc': '50.0', 'leftprimer_start': '76', 'name': '28998221', 'guidecount': 23, 'leftprimer_tm': '60.1789952365', 'product_length': 1446, 'rightprimer_length': '23', 'rightprimer_gc': '47.8260869565', 'leftprimer': 'TGCCACTTCTTGCCATGACA', 'expandedpriming': 50}
{'rightprimer': 'TGTGAAATTGGGATCAGTCTTGC', 'rightprimer_start': '3734', 'rightprimer_tm': '59.4927672525', 'leftprimer_length': '23', 'template_length': 3751, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '68', 'name': '29267071', 'guidecount': 23, 'leftprimer_tm': '60.0571743506', 'product_length': 3666, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'GCTCTGCTTACTATATCGGCACT', 'expandedpriming': 50}
{'rightprimer': 'GGGGGCATCTCAGGGCAT', 'rightprimer_start': '2563', 'rightprimer_tm': '61.5057734704', 'leftprimer_length': '20', 'template_length': 2577, 'leftprimer_gc': '55.0', 'leftprimer_start': '658', 'name': '29324416', 'guidecount': 23, 'leftprimer_tm': '60.9618567488', 'product_length': 1905, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'TAACCATGCACAGCAGCCAG', 'expandedpriming': 20}
{'rightprimer': 'AACTGGGCCGGTGAGTAAAC', 'rightprimer_start': '1677', 'rightprimer_tm': '60.2505967629', 'leftprimer_length': '20', 'template_length': 1978, 'leftprimer_gc': '55.0', 'leftprimer_start': '10', 'name': '30174246', 'guidecount': 23, 'leftprimer_tm': '60.2522387559', 'product_length': 1667, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCCAAGCTAGGCAGAGTGGA', 'expandedpriming': 20}
{'rightprimer': 'GTTCCTTCCTGGTAACCTCCG', 'rightprimer_start': '1900', 'rightprimer_tm': '60.0663582513', 'leftprimer_length': '20', 'template_length': 1894, 'leftprimer_gc': '50.0', 'leftprimer_start': '410', 'name': '30402350', 'guidecount': 23, 'leftprimer_tm': '59.8258383272', 'product_length': 1490, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'GGATTTCGCTGCTGGCAAAT', 'expandedpriming': 0}
{'rightprimer': 'GCGGCCCTGGTAACACTG', 'rightprimer_start': '1978', 'rightprimer_tm': '60.7462774561', 'leftprimer_length': '20', 'template_length': 1974, 'leftprimer_gc': '55.0', 'leftprimer_start': '561', 'name': '30968591', 'guidecount': 23, 'leftprimer_tm': '60.3206829891', 'product_length': 1417, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'TTGTGTCTCGGAGCATGGTG', 'expandedpriming': 0}
{'rightprimer': 'GGTTAGGAAGCCGGAGTCAT', 'rightprimer_start': '2542', 'rightprimer_tm': '59.1686933727', 'leftprimer_length': '23', 'template_length': 2534, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '0', 'name': '31206509', 'guidecount': 23, 'leftprimer_tm': '58.7005150566', 'product_length': 2542, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCTGGGGTATCCTAGTTTCTCTT', 'expandedpriming': 20}
{'rightprimer': 'CAGCACTGTGGTAGCTTCCA', 'rightprimer_start': '2033', 'rightprimer_tm': '59.964683149', 'leftprimer_length': '21', 'template_length': 2627, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '9', 'name': '31330696', 'guidecount': 23, 'leftprimer_tm': '59.7880513796', 'product_length': 2024, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCGTGACCTGGACCCATATTT', 'expandedpriming': 0}
{'rightprimer': 'GAGAATATAGTGGCCCTAGTAAAGG', 'rightprimer_start': '2259', 'rightprimer_tm': '58.0626075171', 'leftprimer_length': '20', 'template_length': 2250, 'leftprimer_gc': '60.0', 'leftprimer_start': '24', 'name': '33188550', 'guidecount': 23, 'leftprimer_tm': '59.5421491004', 'product_length': 2235, 'rightprimer_length': '25', 'rightprimer_gc': '44.0', 'leftprimer': 'CTGTAGGAGTCAAGCCGGAG', 'expandedpriming': 20}
{'rightprimer': 'TGTTGCCCCTGATACTCTGG', 'rightprimer_start': '1336', 'rightprimer_tm': '59.0882306981', 'leftprimer_length': '19', 'template_length': 1549, 'leftprimer_gc': '52.6315789474', 'leftprimer_start': '0', 'name': '33849298', 'guidecount': 23, 'leftprimer_tm': '57.4107679644', 'product_length': 1336, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCCTGGAAGGAAAGGTGGA', 'expandedpriming': 0}
{'rightprimer': 'CTCTGGTGGGTCCCGGTG', 'rightprimer_start': '1513', 'rightprimer_tm': '61.7063727293', 'leftprimer_length': '20', 'template_length': 1528, 'leftprimer_gc': '60.0', 'leftprimer_start': '56', 'name': '34076624', 'guidecount': 23, 'leftprimer_tm': '60.6178204236', 'product_length': 1457, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'CCACTAGCTACCCCAGGTGT', 'expandedpriming': 20}
{'rightprimer': 'GTAGCAATGGGGCTGGGTAG', 'rightprimer_start': '753', 'rightprimer_tm': '60.1790176843', 'leftprimer_length': '24', 'template_length': 775, 'leftprimer_gc': '41.6666666667', 'leftprimer_start': '42', 'name': '35728043', 'guidecount': 23, 'leftprimer_tm': '59.1792836334', 'product_length': 711, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCATCTTGCTCCATAGACAGATTT', 'expandedpriming': 50}
{'rightprimer': 'CTGCTCTAGCTGTCCACTG', 'rightprimer_start': '2113', 'rightprimer_tm': '57.2628726102', 'leftprimer_length': '20', 'template_length': 2110, 'leftprimer_gc': '55.0', 'leftprimer_start': '127', 'name': '36282447', 'guidecount': 23, 'leftprimer_tm': '57.4420804601', 'product_length': 1986, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'CCCATCTTATCAGGACCAGC', 'expandedpriming': 0}
{'rightprimer': 'CTTAGGGCTGGAAAGCACCA', 'rightprimer_start': '1357', 'rightprimer_tm': '59.9618343238', 'leftprimer_length': '21', 'template_length': 1388, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '10', 'name': '37280487', 'guidecount': 23, 'leftprimer_tm': '59.9968817949', 'product_length': 1347, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTTCCCTGGCACACTCATACA', 'expandedpriming': 0}
{'rightprimer': 'CCCCCGGGGCTATAATAACC', 'rightprimer_start': '1263', 'rightprimer_tm': '59.3778636941', 'leftprimer_length': '20', 'template_length': 1261, 'leftprimer_gc': '55.0', 'leftprimer_start': '100', 'name': '562715', 'guidecount': 22, 'leftprimer_tm': '59.5347127812', 'product_length': 1163, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TTATCCCGGGGTTCGTTAGC', 'expandedpriming': 20}
{'rightprimer': 'CACTCTGTGTCTGCCCTCAG', 'rightprimer_start': '1588', 'rightprimer_tm': '60.0375116834', 'leftprimer_length': '20', 'template_length': 1741, 'leftprimer_gc': '50.0', 'leftprimer_start': '243', 'name': '1004200', 'guidecount': 22, 'leftprimer_tm': '59.9652441429', 'product_length': 1345, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TGATGGCTTACAGTGCACGT', 'expandedpriming': 0}
{'rightprimer': 'GTTATTGGGTGATGGGGGCA', 'rightprimer_start': '2121', 'rightprimer_tm': '60.0326147522', 'leftprimer_length': '21', 'template_length': 2373, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '52', 'name': '1202583', 'guidecount': 22, 'leftprimer_tm': '59.9964199684', 'product_length': 2069, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AATCAGAGCACCATGTTCGGT', 'expandedpriming': 0}
{'rightprimer': 'GCTACCTCTGCCCCAATCTG', 'rightprimer_start': '2483', 'rightprimer_tm': '60.1790343683', 'leftprimer_length': '20', 'template_length': 2994, 'leftprimer_gc': '55.0', 'leftprimer_start': '264', 'name': '1928755', 'guidecount': 22, 'leftprimer_tm': '60.5382867959', 'product_length': 2219, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'AAACCCCAGAAGAGCAGTGC', 'expandedpriming': 0}
{'rightprimer': 'CACCAGCGATCCGAACTTCT', 'rightprimer_start': '1208', 'rightprimer_tm': '60.1086145585', 'leftprimer_length': '19', 'template_length': 1464, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '31', 'name': '2007991', 'guidecount': 22, 'leftprimer_tm': '60.0032334696', 'product_length': 1177, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AAGAAAGAGGGAGGCGTGC', 'expandedpriming': 0}
{'rightprimer': 'ACGCTGTGATCTAGCAGAGA', 'rightprimer_start': '1681', 'rightprimer_tm': '58.2435699443', 'leftprimer_length': '22', 'template_length': 1677, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '4', 'name': '2160050', 'guidecount': 22, 'leftprimer_tm': '57.1898090916', 'product_length': 1677, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AGGTCTAGTGTTTGGTCTGATC', 'expandedpriming': 0}
{'rightprimer': 'TAATGTCTGGGTCCGTTGCC', 'rightprimer_start': '2586', 'rightprimer_tm': '60.0358201114', 'leftprimer_length': '20', 'template_length': 2710, 'leftprimer_gc': '55.0', 'leftprimer_start': '39', 'name': '2288687', 'guidecount': 22, 'leftprimer_tm': '60.037232454', 'product_length': 2547, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGTTAGACGGCCTTGAGCAC', 'expandedpriming': 0}
{'rightprimer': 'CGCAGCATACTCCCTTGCTA', 'rightprimer_start': '805', 'rightprimer_tm': '59.8951450311', 'leftprimer_length': '20', 'template_length': 1165, 'leftprimer_gc': '50.0', 'leftprimer_start': '28', 'name': '2825220', 'guidecount': 22, 'leftprimer_tm': '59.8921689425', 'product_length': 777, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCTGCTTACCCGCAACACTT', 'expandedpriming': 0}
{'rightprimer': 'TCCTGGTGTCCGGAGTTTTT', 'rightprimer_start': '1150', 'rightprimer_tm': '59.1587112566', 'leftprimer_length': '20', 'template_length': 1143, 'leftprimer_gc': '55.0', 'leftprimer_start': '56', 'name': '3576841', 'guidecount': 22, 'leftprimer_tm': '59.5982619541', 'product_length': 1094, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CACTGTGTGACTGGGGGAAT', 'expandedpriming': 20}
{'rightprimer': 'GAAGCTGCCCCGGACGGAG', 'rightprimer_start': '621', 'rightprimer_tm': '65.2271177563', 'leftprimer_length': '20', 'template_length': 614, 'leftprimer_gc': '70.0', 'leftprimer_start': '18', 'name': '3824332', 'guidecount': 22, 'leftprimer_tm': '65.1772827625', 'product_length': 603, 'rightprimer_length': '19', 'rightprimer_gc': '73.6842105263', 'leftprimer': 'CACCCCGTCAGTTGTCCCCG', 'expandedpriming': 20}
{'rightprimer': 'GCACAGCCTGTACTTGGTCA', 'rightprimer_start': '1516', 'rightprimer_tm': '60.2502948792', 'leftprimer_length': '21', 'template_length': 1913, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '513', 'name': '3901761', 'guidecount': 22, 'leftprimer_tm': '60.3390552047', 'product_length': 1003, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTATCTGGAACCACTGCACA', 'expandedpriming': 0}
{'rightprimer': 'CATGTGGTATGTCATGGGGAATG', 'rightprimer_start': '2979', 'rightprimer_tm': '59.4290245957', 'leftprimer_length': '20', 'template_length': 3038, 'leftprimer_gc': '55.0', 'leftprimer_start': '42', 'name': '4199013', 'guidecount': 22, 'leftprimer_tm': '59.6763710045', 'product_length': 2937, 'rightprimer_length': '23', 'rightprimer_gc': '47.8260869565', 'leftprimer': 'TTGCCTAAACTCCCCGAGTG', 'expandedpriming': 50}
{'rightprimer': 'CAGGGCTCAGAAATGCTCCA', 'rightprimer_start': '2715', 'rightprimer_tm': '60.0347669151', 'leftprimer_length': '20', 'template_length': 2918, 'leftprimer_gc': '50.0', 'leftprimer_start': '104', 'name': '4433627', 'guidecount': 22, 'leftprimer_tm': '60.1074104683', 'product_length': 2611, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGCCAAAACGGTCACTGACA', 'expandedpriming': 0}
{'rightprimer': 'TGGGCTCAGTTCCTGTATAGA', 'rightprimer_start': '1861', 'rightprimer_tm': '57.6016823756', 'leftprimer_length': '20', 'template_length': 1878, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '4899936', 'guidecount': 22, 'leftprimer_tm': '57.2317470346', 'product_length': 1861, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'CTGTCACCATCCAGCTGTTA', 'expandedpriming': 0}
{'rightprimer': 'TGCCATACAATTGCTGTGCC', 'rightprimer_start': '1594', 'rightprimer_tm': '59.4683425789', 'leftprimer_length': '20', 'template_length': 1605, 'leftprimer_gc': '55.0', 'leftprimer_start': '9', 'name': '5095296', 'guidecount': 22, 'leftprimer_tm': '59.2363477541', 'product_length': 1585, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CCAGGCATGATCCTAGCTGT', 'expandedpriming': 20}
{'rightprimer': 'CACTTAACCCAAACGCCTGC', 'rightprimer_start': '2134', 'rightprimer_tm': '60.0395272033', 'leftprimer_length': '20', 'template_length': 2196, 'leftprimer_gc': '60.0', 'leftprimer_start': '170', 'name': '5559933', 'guidecount': 22, 'leftprimer_tm': '59.8956962875', 'product_length': 1964, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CGTGTATACCCTCCCTGTGC', 'expandedpriming': 0}
{'rightprimer': 'CTTCACCGGCAGGCAAAC', 'rightprimer_start': '2893', 'rightprimer_tm': '59.3550079846', 'leftprimer_length': '18', 'template_length': 2890, 'leftprimer_gc': '55.5555555556', 'leftprimer_start': '2', 'name': '5636466', 'guidecount': 22, 'leftprimer_tm': '56.500673045', 'product_length': 2891, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'CCAGGCCATGGTTACAGT', 'expandedpriming': 0}
{'rightprimer': 'GGAACATCTAGTTTCATAACCACTG', 'rightprimer_start': '1784', 'rightprimer_tm': '57.6912181303', 'leftprimer_length': '20', 'template_length': 1779, 'leftprimer_gc': '50.0', 'leftprimer_start': '174', 'name': '5647519', 'guidecount': 22, 'leftprimer_tm': '58.5539844008', 'product_length': 1610, 'rightprimer_length': '25', 'rightprimer_gc': '40.0', 'leftprimer': 'ACTAGGGCCCAAGTCAAGTT', 'expandedpriming': 20}
{'rightprimer': 'CGGGAGGTTCTATGCGGAAG', 'rightprimer_start': '2128', 'rightprimer_tm': '60.2494889755', 'leftprimer_length': '20', 'template_length': 2130, 'leftprimer_gc': '50.0', 'leftprimer_start': '151', 'name': '5810597', 'guidecount': 22, 'leftprimer_tm': '60.2509013809', 'product_length': 1977, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'AATCTTCCAGCGGCAGTGTT', 'expandedpriming': 20}
{'rightprimer': 'ATGCCCAACTCTCCTGCTTT', 'rightprimer_start': '1133', 'rightprimer_tm': '59.5928613665', 'leftprimer_length': '20', 'template_length': 1966, 'leftprimer_gc': '50.0', 'leftprimer_start': '175', 'name': '5830097', 'guidecount': 22, 'leftprimer_tm': '59.8207664515', 'product_length': 958, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGAAGCCTTCCGTTGTGTCA', 'expandedpriming': 0}
{'rightprimer': 'ACAACTCCCAGGATGCCTAT', 'rightprimer_start': '2016', 'rightprimer_tm': '58.1011373094', 'leftprimer_length': '18', 'template_length': 2019, 'leftprimer_gc': '55.5555555556', 'leftprimer_start': '7', 'name': '5836592', 'guidecount': 22, 'leftprimer_tm': '57.5522663885', 'product_length': 2009, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CCTGGCCAAGGCTGAATT', 'expandedpriming': 0}
{'rightprimer': 'GCCCCTATCCCGAGGAGT', 'rightprimer_start': '2206', 'rightprimer_tm': '59.7982286969', 'leftprimer_length': '20', 'template_length': 2247, 'leftprimer_gc': '55.0', 'leftprimer_start': '32', 'name': '6023791', 'guidecount': 22, 'leftprimer_tm': '59.6931604238', 'product_length': 2174, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'GACATGTGCATCCATCAGCG', 'expandedpriming': 0}
{'rightprimer': 'ACAGACGGCCTATCAGCATG', 'rightprimer_start': '661', 'rightprimer_tm': '59.8944067272', 'leftprimer_length': '20', 'template_length': 803, 'leftprimer_gc': '50.0', 'leftprimer_start': '18', 'name': '6134359', 'guidecount': 22, 'leftprimer_tm': '60.0326147522', 'product_length': 643, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGCCCTGACCCAAACACTTT', 'expandedpriming': 0}
{'rightprimer': 'GTCTGCACTCTAGCCATGGG', 'rightprimer_start': '2443', 'rightprimer_tm': '60.1790618901', 'leftprimer_length': '20', 'template_length': 2433, 'leftprimer_gc': '55.0', 'leftprimer_start': '5', 'name': '6903177', 'guidecount': 22, 'leftprimer_tm': '60.1077029756', 'product_length': 2438, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CGCTACCTGGATTCTTGCCA', 'expandedpriming': 0}
{'rightprimer': 'GGCGTATACGTCCAGGACTC', 'rightprimer_start': '750', 'rightprimer_tm': '59.6897679108', 'leftprimer_length': '20', 'template_length': 736, 'leftprimer_gc': '55.0', 'leftprimer_start': '7', 'name': '6984214', 'guidecount': 22, 'leftprimer_tm': '59.7486464091', 'product_length': 743, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TGATCTGAGCTGCTGGTTCC', 'expandedpriming': 20}
{'rightprimer': 'TGGGGTCCCACTATTCCTCA', 'rightprimer_start': '2147', 'rightprimer_tm': '59.5801347063', 'leftprimer_length': '18', 'template_length': 2140, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '0', 'name': '7069377', 'guidecount': 22, 'leftprimer_tm': '60.4402473858', 'product_length': 2147, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCCCGGGCAAATCTGTCC', 'expandedpriming': 0}
{'rightprimer': 'GGAATGTTGTGCTCCCCAAG', 'rightprimer_start': '2086', 'rightprimer_tm': '59.1093171275', 'leftprimer_length': '20', 'template_length': 2227, 'leftprimer_gc': '55.0', 'leftprimer_start': '12', 'name': '7794610', 'guidecount': 22, 'leftprimer_tm': '59.4297818001', 'product_length': 2074, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGAGGTCCATATCCCAGCCT', 'expandedpriming': 0}
{'rightprimer': 'TACTCGGTGTGCTCTAGGCT', 'rightprimer_start': '3147', 'rightprimer_tm': '60.0343801963', 'leftprimer_length': '20', 'template_length': 3154, 'leftprimer_gc': '55.0', 'leftprimer_start': '488', 'name': '8182277', 'guidecount': 22, 'leftprimer_tm': '59.964683149', 'product_length': 2659, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAGGCAAAAGCCCCCTTTTC', 'expandedpriming': 20}
{'rightprimer': 'TCCAATGAGCACCTAAACCCA', 'rightprimer_start': '1898', 'rightprimer_tm': '59.2920111725', 'leftprimer_length': '20', 'template_length': 3495, 'leftprimer_gc': '60.0', 'leftprimer_start': '38', 'name': '8258268', 'guidecount': 22, 'leftprimer_tm': '59.7494866436', 'product_length': 1860, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'GCCTGACCTACCACTTGGAG', 'expandedpriming': 0}
{'rightprimer': 'CCAATGTGCCAGCCTAGC', 'rightprimer_start': '2267', 'rightprimer_tm': '58.4819968476', 'leftprimer_length': '21', 'template_length': 2285, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '5', 'name': '8321348', 'guidecount': 22, 'leftprimer_tm': '58.4596169996', 'product_length': 2262, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'AGGCTAGATGGGCGATAATGA', 'expandedpriming': 20}
{'rightprimer': 'GTTCCGGCCCAGCATACAG', 'rightprimer_start': '1670', 'rightprimer_tm': '60.8196320409', 'leftprimer_length': '20', 'template_length': 1662, 'leftprimer_gc': '55.0', 'leftprimer_start': '9', 'name': '8696363', 'guidecount': 22, 'leftprimer_tm': '60.392997683', 'product_length': 1661, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'CGGCCCTGCACTTATCAGTT', 'expandedpriming': 20}
{'rightprimer': 'GAGAGCCCCCATGTTGCTTA', 'rightprimer_start': '3661', 'rightprimer_tm': '59.7441096185', 'leftprimer_length': '20', 'template_length': 3851, 'leftprimer_gc': '50.0', 'leftprimer_start': '728', 'name': '8754354', 'guidecount': 22, 'leftprimer_tm': '59.5928613665', 'product_length': 2933, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ATTTCTGCTTGGGTCCTGCT', 'expandedpriming': 0}
{'rightprimer': 'TTTCAGCCAGATGTAGAGTCTTTAA', 'rightprimer_start': '3664', 'rightprimer_tm': '57.9954450993', 'leftprimer_length': '20', 'template_length': 3728, 'leftprimer_gc': '50.0', 'leftprimer_start': '275', 'name': '8810239', 'guidecount': 22, 'leftprimer_tm': '58.0955600734', 'product_length': 3389, 'rightprimer_length': '25', 'rightprimer_gc': '36.0', 'leftprimer': 'ATGAGCCCCTGAGAATCCTT', 'expandedpriming': 0}
{'rightprimer': 'GGGGAGCTCAAACTGGGAAA', 'rightprimer_start': '1973', 'rightprimer_tm': '59.8875877447', 'leftprimer_length': '20', 'template_length': 2215, 'leftprimer_gc': '55.0', 'leftprimer_start': '60', 'name': '9081240', 'guidecount': 22, 'leftprimer_tm': '60.0369521532', 'product_length': 1913, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CGCAGACCACTGAGACCATT', 'expandedpriming': 0}
{'rightprimer': 'GCTTAGCTAGCTGGAAGGTT', 'rightprimer_start': '1900', 'rightprimer_tm': '57.2992779577', 'leftprimer_length': '20', 'template_length': 1896, 'leftprimer_gc': '55.0', 'leftprimer_start': '0', 'name': '9240063', 'guidecount': 22, 'leftprimer_tm': '59.2351101503', 'product_length': 1900, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CTGTCAGTGGTTCCTGGGAA', 'expandedpriming': 0}
{'rightprimer': 'CCCCCCAGACATCTGTGTTT', 'rightprimer_start': '1817', 'rightprimer_tm': '59.5944145384', 'leftprimer_length': '21', 'template_length': 1924, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '119', 'name': '9273333', 'guidecount': 22, 'leftprimer_tm': '59.1720307436', 'product_length': 1698, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCAGTAGATGTCCTGGACACT', 'expandedpriming': 20}
{'rightprimer': 'AAGCTTTCAGGATTGGCGGA', 'rightprimer_start': '2681', 'rightprimer_tm': '59.9618343238', 'leftprimer_length': '20', 'template_length': 2730, 'leftprimer_gc': '50.0', 'leftprimer_start': '242', 'name': '9398391', 'guidecount': 22, 'leftprimer_tm': '59.8908474873', 'product_length': 2439, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TCTGCAACAGACACTGGCAT', 'expandedpriming': 0}
{'rightprimer': 'ACAGGCATGCACATTTCTGC', 'rightprimer_start': '1432', 'rightprimer_tm': '59.7544606186', 'leftprimer_length': '20', 'template_length': 1579, 'leftprimer_gc': '55.0', 'leftprimer_start': '173', 'name': '9999691', 'guidecount': 22, 'leftprimer_tm': '59.6687217718', 'product_length': 1259, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TTATGTTGGCCCTCCTGGTG', 'expandedpriming': 20}
{'rightprimer': 'CCTCCCTCCCAGAGAAGGAA', 'rightprimer_start': '3898', 'rightprimer_tm': '59.9566660826', 'leftprimer_length': '21', 'template_length': 4455, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '281', 'name': '10955917', 'guidecount': 22, 'leftprimer_tm': '60.000162987', 'product_length': 3617, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CGATAGTGGTGCGCCTTATCT', 'expandedpriming': 0}
{'rightprimer': 'CAGACTCCCTCGGGACTACA', 'rightprimer_start': '3627', 'rightprimer_tm': '60.0343801963', 'leftprimer_length': '20', 'template_length': 3632, 'leftprimer_gc': '50.0', 'leftprimer_start': '249', 'name': '11116506', 'guidecount': 22, 'leftprimer_tm': '60.1069178956', 'product_length': 3378, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'ACCTTTTGGAGCGAACACCA', 'expandedpriming': 0}
{'rightprimer': 'AGCAGGCAGTAATCCTCCAAC', 'rightprimer_start': '2145', 'rightprimer_tm': '60.0651258451', 'leftprimer_length': '21', 'template_length': 2167, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '125', 'name': '11175971', 'guidecount': 22, 'leftprimer_tm': '59.9267160304', 'product_length': 2020, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'GTTGGACAAAGTAGGGCCAGA', 'expandedpriming': 0}
{'rightprimer': 'GGAAGTAAGGGTGGCTGTCG', 'rightprimer_start': '1126', 'rightprimer_tm': '60.391262736', 'leftprimer_length': '19', 'template_length': 1853, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '41', 'name': '11184816', 'guidecount': 22, 'leftprimer_tm': '60.5297128768', 'product_length': 1085, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TTCGTTGCCACCCCTTTCC', 'expandedpriming': 0}
{'rightprimer': 'ACACCCTAGGATCATGCCCT', 'rightprimer_start': '1229', 'rightprimer_tm': '60.0296806863', 'leftprimer_length': '20', 'template_length': 1235, 'leftprimer_gc': '60.0', 'leftprimer_start': '10', 'name': '11242170', 'guidecount': 22, 'leftprimer_tm': '60.2520121956', 'product_length': 1219, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGAGGTGACACCAGGGAGAG', 'expandedpriming': 20}
{'rightprimer': 'GGTCCCAGCTCCTGATAGGA', 'rightprimer_start': '3160', 'rightprimer_tm': '60.105452598', 'leftprimer_length': '20', 'template_length': 3177, 'leftprimer_gc': '55.0', 'leftprimer_start': '273', 'name': '11633752', 'guidecount': 22, 'leftprimer_tm': '60.1075082218', 'product_length': 2887, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCCGCATGTCATGTTCCTCT', 'expandedpriming': 0}
{'rightprimer': 'TCTGCCGCGTCTATTGACTC', 'rightprimer_start': '1968', 'rightprimer_tm': '59.8993181144', 'leftprimer_length': '20', 'template_length': 2489, 'leftprimer_gc': '50.0', 'leftprimer_start': '117', 'name': '11712903', 'guidecount': 22, 'leftprimer_tm': '59.8960626217', 'product_length': 1851, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGTTTGGCAAAGCGGAAAGG', 'expandedpriming': 0}
{'rightprimer': 'CCTCTGAGTGCTCTAATGCTCT', 'rightprimer_start': '1876', 'rightprimer_tm': '59.3053724624', 'leftprimer_length': '23', 'template_length': 1882, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '819', 'name': '11868285', 'guidecount': 22, 'leftprimer_tm': '59.677172345', 'product_length': 1057, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'TCGTGTAAAGAACTAAGCCCAGT', 'expandedpriming': 0}
{'rightprimer': 'GAGGTACTGGCCGGGTACT', 'rightprimer_start': '2102', 'rightprimer_tm': '60.3809201113', 'leftprimer_length': '20', 'template_length': 2086, 'leftprimer_gc': '60.0', 'leftprimer_start': '8', 'name': '13156893', 'guidecount': 22, 'leftprimer_tm': '60.0377898474', 'product_length': 2094, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'GAGTGGTCACTGGACAGAGC', 'expandedpriming': 0}
{'rightprimer': 'CAGGTTGCTGCCACCTGT', 'rightprimer_start': '1260', 'rightprimer_tm': '60.2033937865', 'leftprimer_length': '23', 'template_length': 1354, 'leftprimer_gc': '52.1739130435', 'leftprimer_start': '2', 'name': '13890311', 'guidecount': 22, 'leftprimer_tm': '60.307974677', 'product_length': 1258, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'CAGGAATAGCCCATGATACAGGG', 'expandedpriming': 20}
{'rightprimer': 'AGAACGTCTGGGAGAGGGAC', 'rightprimer_start': '2008', 'rightprimer_tm': '60.6124529676', 'leftprimer_length': '19', 'template_length': 2189, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '137', 'name': '14715859', 'guidecount': 22, 'leftprimer_tm': '60.689232787', 'product_length': 1871, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CGGTCTCCTCCCCCATGAA', 'expandedpriming': 20}
{'rightprimer': 'GAAAGGTTAGCAGCTGCAGC', 'rightprimer_start': '1991', 'rightprimer_tm': '59.8301057532', 'leftprimer_length': '24', 'template_length': 2572, 'leftprimer_gc': '41.6666666667', 'leftprimer_start': '83', 'name': '15035034', 'guidecount': 22, 'leftprimer_tm': '59.4714040477', 'product_length': 1908, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACACAGCATAACTTAGAGGACAGT', 'expandedpriming': 0}
{'rightprimer': 'ATGGCAGAAGCACAATGGCT', 'rightprimer_start': '1986', 'rightprimer_tm': '60.6138525567', 'leftprimer_length': '20', 'template_length': 2084, 'leftprimer_gc': '55.0', 'leftprimer_start': '137', 'name': '15267791', 'guidecount': 22, 'leftprimer_tm': '60.3252964294', 'product_length': 1849, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'AGACCGCCAACATAGAGGGA', 'expandedpriming': 0}
{'rightprimer': 'CGTTACGAGACAGTTGCCTG', 'rightprimer_start': '2638', 'rightprimer_tm': '58.9392867253', 'leftprimer_length': '25', 'template_length': 3072, 'leftprimer_gc': '40.0', 'leftprimer_start': '82', 'name': '15557558', 'guidecount': 22, 'leftprimer_tm': '58.8749711079', 'product_length': 2556, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCCCAGTTCACTTCAGATGAATAAT', 'expandedpriming': 0}
{'rightprimer': 'CCCTAGTCATGTGCTTCACCT', 'rightprimer_start': '2652', 'rightprimer_tm': '59.4430890899', 'leftprimer_length': '25', 'template_length': 2652, 'leftprimer_gc': '36.0', 'leftprimer_start': '6', 'name': '16358579', 'guidecount': 22, 'leftprimer_tm': '59.119667994', 'product_length': 2646, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'AGCTTTGACGATTTTATTAGCAGGA', 'expandedpriming': 20}
{'rightprimer': 'GTGATTGGCCAGTGCAAATGA', 'rightprimer_start': '1133', 'rightprimer_tm': '59.7269523822', 'leftprimer_length': '18', 'template_length': 1218, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '78', 'name': '17993681', 'guidecount': 22, 'leftprimer_tm': '60.3575169935', 'product_length': 1055, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'GTCCGGACACTGCCACAG', 'expandedpriming': 20}
{'rightprimer': 'TGTGCAGTTCTAGTCTGTGTGA', 'rightprimer_start': '3449', 'rightprimer_tm': '59.306925956', 'leftprimer_length': '20', 'template_length': 3451, 'leftprimer_gc': '50.0', 'leftprimer_start': '4', 'name': '18096199', 'guidecount': 22, 'leftprimer_tm': '59.0939342833', 'product_length': 3445, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'TTACCTGGCATGTAAGCGGA', 'expandedpriming': 0}
{'rightprimer': 'CCGCTAGTCTGATATGGAGTCAC', 'rightprimer_start': '2572', 'rightprimer_tm': '60.0578761397', 'leftprimer_length': '21', 'template_length': 2580, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '12', 'name': '18603070', 'guidecount': 22, 'leftprimer_tm': '59.9992694216', 'product_length': 2560, 'rightprimer_length': '23', 'rightprimer_gc': '52.1739130435', 'leftprimer': 'GCAACTTGGCTTGGCACATAA', 'expandedpriming': 20}
{'rightprimer': 'CCTTCTGGGTATTTGCTGAATTC', 'rightprimer_start': '1138', 'rightprimer_tm': '57.745661399', 'leftprimer_length': '25', 'template_length': 1191, 'leftprimer_gc': '36.0', 'leftprimer_start': '46', 'name': '19082511', 'guidecount': 22, 'leftprimer_tm': '58.1303051449', 'product_length': 1092, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'TCTTTATGCACTTAGGTGTTATCGA', 'expandedpriming': 50}
{'rightprimer': 'ATCACCCTGAGGCCTATGGT', 'rightprimer_start': '1866', 'rightprimer_tm': '60.0296806863', 'leftprimer_length': '20', 'template_length': 2142, 'leftprimer_gc': '55.0', 'leftprimer_start': '280', 'name': '19161447', 'guidecount': 22, 'leftprimer_tm': '60.0356297333', 'product_length': 1586, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AGGAGTTGCTTAGGCTGCTG', 'expandedpriming': 0}
{'rightprimer': 'ATTACAGCAGGAGGGACCCT', 'rightprimer_start': '3328', 'rightprimer_tm': '59.9551448463', 'leftprimer_length': '20', 'template_length': 3593, 'leftprimer_gc': '50.0', 'leftprimer_start': '301', 'name': '21134775', 'guidecount': 22, 'leftprimer_tm': '59.9590583175', 'product_length': 3027, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGTGCAACCTATGGCCCAAT', 'expandedpriming': 0}
{'rightprimer': 'TCCCCACTAGGCCAAAGAAC', 'rightprimer_start': '1978', 'rightprimer_tm': '59.3034989261', 'leftprimer_length': '20', 'template_length': 1983, 'leftprimer_gc': '50.0', 'leftprimer_start': '20', 'name': '21185401', 'guidecount': 22, 'leftprimer_tm': '59.4728869538', 'product_length': 1958, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCATCATGCCTGCGAAACAA', 'expandedpriming': 20}
{'rightprimer': 'CCCCCTTGGCAATTACCTCA', 'rightprimer_start': '1948', 'rightprimer_tm': '59.6653235453', 'leftprimer_length': '20', 'template_length': 1975, 'leftprimer_gc': '50.0', 'leftprimer_start': '59', 'name': '22008082', 'guidecount': 22, 'leftprimer_tm': '59.6199764289', 'product_length': 1889, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CGCACATGTTGGTTGACACA', 'expandedpriming': 0}
{'rightprimer': 'CGCAGGATGAAATGCCTGTG', 'rightprimer_start': '3395', 'rightprimer_tm': '59.9000315622', 'leftprimer_length': '24', 'template_length': 3575, 'leftprimer_gc': '45.8333333333', 'leftprimer_start': '3', 'name': '23234121', 'guidecount': 22, 'leftprimer_tm': '59.9049641715', 'product_length': 3392, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTGCCTTTACTCATAGTTTCAGCC', 'expandedpriming': 0}
{'rightprimer': 'AAGGAACTCTCCCTCAGCCT', 'rightprimer_start': '1004', 'rightprimer_tm': '59.8818560623', 'leftprimer_length': '20', 'template_length': 1013, 'leftprimer_gc': '55.0', 'leftprimer_start': '13', 'name': '23359304', 'guidecount': 22, 'leftprimer_tm': '59.8914152912', 'product_length': 991, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGCTGACAAAGGGACACAGA', 'expandedpriming': 0}
{'rightprimer': 'ACCAAGTTGACAGCTGTGAGT', 'rightprimer_start': '1879', 'rightprimer_tm': '59.7895218263', 'leftprimer_length': '24', 'template_length': 1894, 'leftprimer_gc': '45.8333333333', 'leftprimer_start': '12', 'name': '23733600', 'guidecount': 22, 'leftprimer_tm': '60.0808629419', 'product_length': 1867, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'CTGGTATCAGCCTCCTTACAAACT', 'expandedpriming': 20}
{'rightprimer': 'TGCTTCTTACTTGGGCTGGG', 'rightprimer_start': '2083', 'rightprimer_tm': '59.9618343238', 'leftprimer_length': '21', 'template_length': 2133, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '37', 'name': '24198790', 'guidecount': 22, 'leftprimer_tm': '59.8521827873', 'product_length': 2046, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AATTCTCTGTGGGGGTGCTTT', 'expandedpriming': 50}
{'rightprimer': 'GCTTAGCATTTGCATTCCCGT', 'rightprimer_start': '3560', 'rightprimer_tm': '59.8650541541', 'leftprimer_length': '20', 'template_length': 3580, 'leftprimer_gc': '60.0', 'leftprimer_start': '379', 'name': '24320985', 'guidecount': 22, 'leftprimer_tm': '59.4581373935', 'product_length': 3181, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'GTAGGATCACACCTGTGGGG', 'expandedpriming': 0}
{'rightprimer': 'GGCCTACCCTGGTTCCAATA', 'rightprimer_start': '1808', 'rightprimer_tm': '58.7811560273', 'leftprimer_length': '25', 'template_length': 1791, 'leftprimer_gc': '40.0', 'leftprimer_start': '0', 'name': '24568010', 'guidecount': 22, 'leftprimer_tm': '58.3969048382', 'product_length': 1808, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCATTTTCACTTACTAGACACTTGG', 'expandedpriming': 0}
{'rightprimer': 'GGGCCCTGGTGCAGCTGC', 'rightprimer_start': '1117', 'rightprimer_tm': '66.4574008449', 'leftprimer_length': '22', 'template_length': 1109, 'leftprimer_gc': '54.5454545455', 'leftprimer_start': '2', 'name': '25439417', 'guidecount': 22, 'leftprimer_tm': '61.7486313169', 'product_length': 1115, 'rightprimer_length': '18', 'rightprimer_gc': '77.7777777778', 'leftprimer': 'GTTCTCCTGGAGCCTGATTGGA', 'expandedpriming': 0}
{'rightprimer': 'GCAAGAGCCAAGCTTAGCAC', 'rightprimer_start': '2436', 'rightprimer_tm': '59.8301057532', 'leftprimer_length': '20', 'template_length': 2694, 'leftprimer_gc': '60.0', 'leftprimer_start': '423', 'name': '25531625', 'guidecount': 22, 'leftprimer_tm': '60.032416005', 'product_length': 2013, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTACCACTGGACCCCTCTA', 'expandedpriming': 0}
{'rightprimer': 'GACAGGGGAGTATGCAACAGA', 'rightprimer_start': '1519', 'rightprimer_tm': '59.4449252725', 'leftprimer_length': '20', 'template_length': 1551, 'leftprimer_gc': '55.0', 'leftprimer_start': '92', 'name': '25670276', 'guidecount': 22, 'leftprimer_tm': '59.5944145384', 'product_length': 1427, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'CCCTGTGGATGGAGGTTGTT', 'expandedpriming': 0}
{'rightprimer': 'GCCTTACCTGCCCTCTTGTT', 'rightprimer_start': '2628', 'rightprimer_tm': '59.9616899326', 'leftprimer_length': '20', 'template_length': 2683, 'leftprimer_gc': '55.0', 'leftprimer_start': '75', 'name': '25960068', 'guidecount': 22, 'leftprimer_tm': '59.9641192616', 'product_length': 2553, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGAGGGTTGCAAGAGCTCAG', 'expandedpriming': 0}
{'rightprimer': 'CATCTGCTCCCAGGATTCCA', 'rightprimer_start': '1172', 'rightprimer_tm': '59.1580387942', 'leftprimer_length': '24', 'template_length': 1164, 'leftprimer_gc': '41.6666666667', 'leftprimer_start': '1', 'name': '25967549', 'guidecount': 22, 'leftprimer_tm': '58.8212029937', 'product_length': 1171, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCTACTTTTGAATCTAGACAGCA', 'expandedpriming': 0}
{'rightprimer': 'GTTCGGAGCCTGGTTACCAA', 'rightprimer_start': '1660', 'rightprimer_tm': '59.9642605059', 'leftprimer_length': '20', 'template_length': 1659, 'leftprimer_gc': '55.0', 'leftprimer_start': '1', 'name': '26468648', 'guidecount': 22, 'leftprimer_tm': '59.9638362258', 'product_length': 1659, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CACTGGTCCGGTGATCCAAA', 'expandedpriming': 0}
{'rightprimer': 'AGCGGGTCCCTTTATGGTTG', 'rightprimer_start': '998', 'rightprimer_tm': '60.0344770646', 'leftprimer_length': '20', 'template_length': 1447, 'leftprimer_gc': '60.0', 'leftprimer_start': '36', 'name': '26775655', 'guidecount': 22, 'leftprimer_tm': '60.3207705649', 'product_length': 962, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTCTTTCGGGTGACTGGGTG', 'expandedpriming': 50}
{'rightprimer': 'ACCCCAACCATGGCAAGAAA', 'rightprimer_start': '3190', 'rightprimer_tm': '60.1052460662', 'leftprimer_length': '19', 'template_length': 3265, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '30', 'name': '27855683', 'guidecount': 22, 'leftprimer_tm': '60.0038387248', 'product_length': 3160, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TCCCTGGTAGCAACGCTTG', 'expandedpriming': 20}
{'rightprimer': 'TGGGCTAGAGAACACATCAGT', 'rightprimer_start': '1894', 'rightprimer_tm': '58.4596169996', 'leftprimer_length': '21', 'template_length': 1901, 'leftprimer_gc': '47.619047619', 'leftprimer_start': '22', 'name': '28018403', 'guidecount': 22, 'leftprimer_tm': '59.4370579149', 'product_length': 1872, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'TCATCTGAATTCAGCAGGGCT', 'expandedpriming': 20}
{'rightprimer': 'ACACCGGACCTGTAAAGCTAA', 'rightprimer_start': '983', 'rightprimer_tm': '59.0272054144', 'leftprimer_length': '20', 'template_length': 1040, 'leftprimer_gc': '55.0', 'leftprimer_start': '386', 'name': '28609410', 'guidecount': 22, 'leftprimer_tm': '59.3846689167', 'product_length': 597, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'CCAGCAGCTTCTAGGAACCA', 'expandedpriming': 20}
{'rightprimer': 'TTCCTGCTGGTGGCTTTGAT', 'rightprimer_start': '3480', 'rightprimer_tm': '59.8870049386', 'leftprimer_length': '20', 'template_length': 4062, 'leftprimer_gc': '55.0', 'leftprimer_start': '317', 'name': '28746159', 'guidecount': 22, 'leftprimer_tm': '59.7617113501', 'product_length': 3163, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GTTTGGTGCCTGTGTGATCG', 'expandedpriming': 0}
{'rightprimer': 'GTGTACCAAGCCTCTCTAGG', 'rightprimer_start': '1235', 'rightprimer_tm': '56.7404342365', 'leftprimer_length': '21', 'template_length': 1218, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '2', 'name': '29085370', 'guidecount': 22, 'leftprimer_tm': '57.2629676292', 'product_length': 1233, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTATCTTCTGCACTAGGGGAC', 'expandedpriming': 0}
{'rightprimer': 'CCAGGAAATGGTCGCTAGCT', 'rightprimer_start': '2522', 'rightprimer_tm': '59.8210001113', 'leftprimer_length': '20', 'template_length': 2532, 'leftprimer_gc': '55.0', 'leftprimer_start': '5', 'name': '29273998', 'guidecount': 22, 'leftprimer_tm': '60.1060149679', 'product_length': 2517, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCCTAGGGACAATGGCAAT', 'expandedpriming': 20}
{'rightprimer': 'GGCTGTCTGTGGATGTGGTT', 'rightprimer_start': '1589', 'rightprimer_tm': '60.2509013809', 'leftprimer_length': '18', 'template_length': 1957, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '2', 'name': '29652275', 'guidecount': 22, 'leftprimer_tm': '61.8301920086', 'product_length': 1587, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTTGGCCCGGCAATGCTC', 'expandedpriming': 0}
{'rightprimer': 'TCCTCACCTGGTCTTCACCT', 'rightprimer_start': '1573', 'rightprimer_tm': '59.8089359029', 'leftprimer_length': '18', 'template_length': 1568, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '4', 'name': '30194199', 'guidecount': 22, 'leftprimer_tm': '59.5478859134', 'product_length': 1569, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCAGGAGGAGGGGTGAGT', 'expandedpriming': 0}
{'rightprimer': 'GCTTGGATGATCCCAACCCA', 'rightprimer_start': '2584', 'rightprimer_tm': '60.0332078652', 'leftprimer_length': '20', 'template_length': 3319, 'leftprimer_gc': '55.0', 'leftprimer_start': '571', 'name': '30453294', 'guidecount': 22, 'leftprimer_tm': '60.0347669151', 'product_length': 2013, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TTGCTCTCATCTGGCCAAGG', 'expandedpriming': 0}
{'rightprimer': 'CCCCGGTTTGTTTGTGGAAG', 'rightprimer_start': '2570', 'rightprimer_tm': '59.6124347731', 'leftprimer_length': '21', 'template_length': 2561, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '21', 'name': '30520640', 'guidecount': 22, 'leftprimer_tm': '59.252644816', 'product_length': 2549, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTGGATTCAGAATGTGGCTGG', 'expandedpriming': 20}
{'rightprimer': 'TCTCAAGTCTGTGGCCACTG', 'rightprimer_start': '927', 'rightprimer_tm': '59.6058079192', 'leftprimer_length': '21', 'template_length': 1306, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '47', 'name': '30559434', 'guidecount': 22, 'leftprimer_tm': '59.6456323878', 'product_length': 880, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGGGCAAAGTAAGGGAGAAGG', 'expandedpriming': 0}
{'rightprimer': 'GCCCAGGTAAGCATATCCAGT', 'rightprimer_start': '1853', 'rightprimer_tm': '59.5783544198', 'leftprimer_length': '20', 'template_length': 1867, 'leftprimer_gc': '55.0', 'leftprimer_start': '424', 'name': '30942709', 'guidecount': 22, 'leftprimer_tm': '59.6024364633', 'product_length': 1429, 'rightprimer_length': '21', 'rightprimer_gc': '52.380952381', 'leftprimer': 'TAACAACTGACCGGTTCCCC', 'expandedpriming': 20}
{'rightprimer': 'GGGGGGAGGAAATTGTAGGTC', 'rightprimer_start': '855', 'rightprimer_tm': '59.7853268439', 'leftprimer_length': '23', 'template_length': 838, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '114', 'name': '31036500', 'guidecount': 22, 'leftprimer_tm': '59.4816971721', 'product_length': 741, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'TGAGGTTCCTAAAGTTAGAGGCC', 'expandedpriming': 0}
{'rightprimer': 'TCTTTTTTTGGTTAGCCAGGAAGT', 'rightprimer_start': '2950', 'rightprimer_tm': '59.0401610744', 'leftprimer_length': '20', 'template_length': 2940, 'leftprimer_gc': '55.0', 'leftprimer_start': '98', 'name': '31139421', 'guidecount': 22, 'leftprimer_tm': '58.6800526148', 'product_length': 2852, 'rightprimer_length': '24', 'rightprimer_gc': '37.5', 'leftprimer': 'TGAGGATCAGAGTAGGGGGT', 'expandedpriming': 20}
{'rightprimer': 'GGCCATCCTGGAAGACCATT', 'rightprimer_start': '2101', 'rightprimer_tm': '59.740061527', 'leftprimer_length': '20', 'template_length': 2093, 'leftprimer_gc': '60.0', 'leftprimer_start': '1025', 'name': '31412782', 'guidecount': 22, 'leftprimer_tm': '59.7522641156', 'product_length': 1076, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTGGCCTCCTAGCGATATGG', 'expandedpriming': 20}
{'rightprimer': 'CCCTATGACCCTGGTTTATTTCTCT', 'rightprimer_start': '3615', 'rightprimer_tm': '59.8671836104', 'leftprimer_length': '20', 'template_length': 3607, 'leftprimer_gc': '50.0', 'leftprimer_start': '551', 'name': '32816681', 'guidecount': 22, 'leftprimer_tm': '59.8955127698', 'product_length': 3064, 'rightprimer_length': '25', 'rightprimer_gc': '44.0', 'leftprimer': 'TGCGACCCAGTTGTTTCGTA', 'expandedpriming': 20}
{'rightprimer': 'CAGGCCAGGGAAGATGGATG', 'rightprimer_start': '3244', 'rightprimer_tm': '60.1789980552', 'leftprimer_length': '20', 'template_length': 3240, 'leftprimer_gc': '60.0', 'leftprimer_start': '363', 'name': '33083897', 'guidecount': 22, 'leftprimer_tm': '59.816268945', 'product_length': 2881, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GGATAGGAACCCAAGCCAGG', 'expandedpriming': 20}
{'rightprimer': 'TTGTTTGTCCCAGGCTGG', 'rightprimer_start': '2095', 'rightprimer_tm': '57.4029681846', 'leftprimer_length': '20', 'template_length': 2087, 'leftprimer_gc': '55.0', 'leftprimer_start': '7', 'name': '33161824', 'guidecount': 22, 'leftprimer_tm': '58.6063968376', 'product_length': 2088, 'rightprimer_length': '18', 'rightprimer_gc': '55.5555555556', 'leftprimer': 'GCAAGGGGCTAGAATGAACC', 'expandedpriming': 0}
{'rightprimer': 'CCCTGCAAGCTGGATTTTGT', 'rightprimer_start': '1374', 'rightprimer_tm': '59.0286136971', 'leftprimer_length': '22', 'template_length': 1459, 'leftprimer_gc': '40.9090909091', 'leftprimer_start': '80', 'name': '33246807', 'guidecount': 22, 'leftprimer_tm': '56.9476810645', 'product_length': 1294, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TCCATTTGGCAAGTGATACAAG', 'expandedpriming': 0}
{'rightprimer': 'GGCCCCTAAAGCTTATGGCA', 'rightprimer_start': '1372', 'rightprimer_tm': '60.1062174577', 'leftprimer_length': '20', 'template_length': 1665, 'leftprimer_gc': '55.0', 'leftprimer_start': '473', 'name': '33259562', 'guidecount': 22, 'leftprimer_tm': '60.1086619282', 'product_length': 899, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGTGACAGAGCACATAGGCG', 'expandedpriming': 0}
{'rightprimer': 'TAACGTGCCTGCCAAAAAGC', 'rightprimer_start': '2504', 'rightprimer_tm': '59.9685505702', 'leftprimer_length': '20', 'template_length': 3245, 'leftprimer_gc': '60.0', 'leftprimer_start': '154', 'name': '33501455', 'guidecount': 22, 'leftprimer_tm': '60.0310099617', 'product_length': 2350, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGGGTCTGAGGGGACATAGT', 'expandedpriming': 0}
{'rightprimer': 'AGCTATTTGGAGCCAGAGCA', 'rightprimer_start': '2452', 'rightprimer_tm': '59.0853564507', 'leftprimer_length': '18', 'template_length': 2720, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '0', 'name': '34280029', 'guidecount': 22, 'leftprimer_tm': '59.1035569955', 'product_length': 2452, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CTCTGCTCCGGGTCCTTC', 'expandedpriming': 0}
{'rightprimer': 'TGGCATCAGTAAACCATGTCTG', 'rightprimer_start': '1440', 'rightprimer_tm': '58.6536852945', 'leftprimer_length': '20', 'template_length': 1680, 'leftprimer_gc': '55.0', 'leftprimer_start': '10', 'name': '35468131', 'guidecount': 22, 'leftprimer_tm': '58.8937149101', 'product_length': 1430, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'CAGCAACTAATTCCTGCCCC', 'expandedpriming': 0}
{'rightprimer': 'ACTAGATTGTAGGCAATGGGC', 'rightprimer_start': '1106', 'rightprimer_tm': '57.7853042222', 'leftprimer_length': '20', 'template_length': 1093, 'leftprimer_gc': '55.0', 'leftprimer_start': '5', 'name': '35533991', 'guidecount': 22, 'leftprimer_tm': '59.100280215', 'product_length': 1101, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'CCCCCACTAGTTAACGCAGA', 'expandedpriming': 20}
{'rightprimer': 'GCCCCCAAGCTTACAATTGC', 'rightprimer_start': '1546', 'rightprimer_tm': '60.1081855111', 'leftprimer_length': '18', 'template_length': 1679, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '12', 'name': '35651165', 'guidecount': 22, 'leftprimer_tm': '60.1245702892', 'product_length': 1534, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCGGGCTGAACAGATGGG', 'expandedpriming': 0}
{'rightprimer': 'GCAAGCCTTCTGCCACTTAT', 'rightprimer_start': '1080', 'rightprimer_tm': '58.5320801109', 'leftprimer_length': '20', 'template_length': 1063, 'leftprimer_gc': '45.0', 'leftprimer_start': '37', 'name': '37222204', 'guidecount': 22, 'leftprimer_tm': '58.6214693179', 'product_length': 1043, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGACGTTGCTTGCAGAACTT', 'expandedpriming': 0}
{'rightprimer': 'CATGGACACACCGGTAAAAAA', 'rightprimer_start': '872', 'rightprimer_tm': '57.0077401785', 'leftprimer_length': '18', 'template_length': 864, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '5', 'name': '37443288', 'guidecount': 22, 'leftprimer_tm': '59.7326758701', 'product_length': 867, 'rightprimer_length': '21', 'rightprimer_gc': '42.8571428571', 'leftprimer': 'GAGGAGCTGTGTCTGCCC', 'expandedpriming': 0}
{'rightprimer': 'AGAATAACGCTCAGTGCCCC', 'rightprimer_start': '2239', 'rightprimer_tm': '60.107654381', 'leftprimer_length': '19', 'template_length': 2674, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '148', 'name': '37623369', 'guidecount': 22, 'leftprimer_tm': '60.9939483013', 'product_length': 2091, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTAACGAACCCCCCTCCT', 'expandedpriming': 0}
{'rightprimer': 'ACGTCTCATAGTCGCCCGTA', 'rightprimer_start': '763', 'rightprimer_tm': '60.4625806915', 'leftprimer_length': '20', 'template_length': 833, 'leftprimer_gc': '60.0', 'leftprimer_start': '125', 'name': '37671872', 'guidecount': 22, 'leftprimer_tm': '60.5351020374', 'product_length': 638, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GTGGGGGTGCATAGGAATCG', 'expandedpriming': 0}
{'rightprimer': 'CACCCCCGAAAAGAGAGGAG', 'rightprimer_start': '1899', 'rightprimer_tm': '59.7494866436', 'leftprimer_length': '22', 'template_length': 2034, 'leftprimer_gc': '50.0', 'leftprimer_start': '73', 'name': '37777367', 'guidecount': 22, 'leftprimer_tm': '59.6366410109', 'product_length': 1826, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCCAATTTAGGAGGCCTTGATG', 'expandedpriming': 0}
{'rightprimer': 'GGAACTGCTCTGTTGCATGC', 'rightprimer_start': '1188', 'rightprimer_tm': '60.1094117572', 'leftprimer_length': '20', 'template_length': 1183, 'leftprimer_gc': '55.0', 'leftprimer_start': '21', 'name': '38131258', 'guidecount': 22, 'leftprimer_tm': '60.3231780629', 'product_length': 1167, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CCATTGAACCCTCAGCAGCT', 'expandedpriming': 20}
{'rightprimer': 'CCCCAATACCGGTCTCTTGC', 'rightprimer_start': '1771', 'rightprimer_tm': '60.4638331732', 'leftprimer_length': '20', 'template_length': 1757, 'leftprimer_gc': '55.0', 'leftprimer_start': '191', 'name': '25402', 'guidecount': 21, 'leftprimer_tm': '60.2503378394', 'product_length': 1580, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GGGTTTCTGAGCCAAGTCGT', 'expandedpriming': 0}
{'rightprimer': 'CCTGTGTGAAAGCCCTGACA', 'rightprimer_start': '1368', 'rightprimer_tm': '60.1790204739', 'leftprimer_length': '19', 'template_length': 1352, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '8', 'name': '30009', 'guidecount': 21, 'leftprimer_tm': '60.1530863724', 'product_length': 1360, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTGGGGCTCACTGTATGGC', 'expandedpriming': 20}
{'rightprimer': 'CAACACAAACCTCTCGGGGG', 'rightprimer_start': '2390', 'rightprimer_tm': '60.8891864881', 'leftprimer_length': '19', 'template_length': 2410, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '0', 'name': '86862', 'guidecount': 21, 'leftprimer_tm': '61.0609799508', 'product_length': 2390, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCTAGGAAGGCCTCGCAGT', 'expandedpriming': 0}
{'rightprimer': 'ACTTTATGCGCAGTTGTCGC', 'rightprimer_start': '2933', 'rightprimer_tm': '59.8340556501', 'leftprimer_length': '20', 'template_length': 2932, 'leftprimer_gc': '55.0', 'leftprimer_start': '79', 'name': '158036', 'guidecount': 21, 'leftprimer_tm': '59.8804391112', 'product_length': 2854, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'TGGGAAGTAGGTGGGGCTTA', 'expandedpriming': 20}
{'rightprimer': 'GCTGCGCTGTATTCCAATGG', 'rightprimer_start': '1476', 'rightprimer_tm': '59.9701664704', 'leftprimer_length': '21', 'template_length': 1660, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '6', 'name': '723702', 'guidecount': 21, 'leftprimer_tm': '60.0676354483', 'product_length': 1470, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAGCCCACATTAACTGCCAAC', 'expandedpriming': 20}
{'rightprimer': 'GATTAGGAACATGGCCGCCA', 'rightprimer_start': '984', 'rightprimer_tm': '60.4654601115', 'leftprimer_length': '20', 'template_length': 1018, 'leftprimer_gc': '50.0', 'leftprimer_start': '350', 'name': '944011', 'guidecount': 21, 'leftprimer_tm': '60.3242753481', 'product_length': 634, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACCTGCTGTGTGGTGGTTTT', 'expandedpriming': 50}
{'rightprimer': 'CTCTAGATACAGGGGCCGGT', 'rightprimer_start': '1061', 'rightprimer_tm': '60.1789582565', 'leftprimer_length': '22', 'template_length': 1061, 'leftprimer_gc': '50.0', 'leftprimer_start': '95', 'name': '1038391', 'guidecount': 21, 'leftprimer_tm': '60.3548050016', 'product_length': 966, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GCGACATTGGGAAGTAGAACCT', 'expandedpriming': 20}
{'rightprimer': 'GCCCGAGGAGTGAGTTTTCA', 'rightprimer_start': '2574', 'rightprimer_tm': '59.9651041645', 'leftprimer_length': '20', 'template_length': 2757, 'leftprimer_gc': '55.0', 'leftprimer_start': '4', 'name': '1039601', 'guidecount': 21, 'leftprimer_tm': '59.8186498723', 'product_length': 2570, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCTACTAGCCTGCCATTGGT', 'expandedpriming': 0}
{'rightprimer': 'TGGCCAACTCCTAGACCCAT', 'rightprimer_start': '1307', 'rightprimer_tm': '60.2531601443', 'leftprimer_length': '20', 'template_length': 1299, 'leftprimer_gc': '50.0', 'leftprimer_start': '96', 'name': '1079541', 'guidecount': 21, 'leftprimer_tm': '60.038343004', 'product_length': 1211, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCACCGCACCATTTCACATT', 'expandedpriming': 0}
{'rightprimer': 'GGGGTGCAGACTAGTTACTCC', 'rightprimer_start': '1906', 'rightprimer_tm': '59.5186372576', 'leftprimer_length': '20', 'template_length': 1898, 'leftprimer_gc': '55.0', 'leftprimer_start': '30', 'name': '1462457', 'guidecount': 21, 'leftprimer_tm': '59.5561311367', 'product_length': 1876, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'CGCCACACACTATTCACAGC', 'expandedpriming': 0}
{'rightprimer': 'GCCATCGTTCCTGTTGCAAG', 'rightprimer_start': '1494', 'rightprimer_tm': '60.1095968673', 'leftprimer_length': '23', 'template_length': 2162, 'leftprimer_gc': '52.1739130435', 'leftprimer_start': '0', 'name': '2019379', 'guidecount': 21, 'leftprimer_tm': '60.1179630988', 'product_length': 1494, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGCACAGGTACTAGAGTAGGAGA', 'expandedpriming': 0}
{'rightprimer': 'CTTCCCCGGGTCTGAGCC', 'rightprimer_start': '1516', 'rightprimer_tm': '61.7870012088', 'leftprimer_length': '21', 'template_length': 1512, 'leftprimer_gc': '57.1428571429', 'leftprimer_start': '113', 'name': '2038604', 'guidecount': 21, 'leftprimer_tm': '61.3534096835', 'product_length': 1403, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'GCTTTACCCTGGCAATACCGG', 'expandedpriming': 20}
{'rightprimer': 'AGATTGACAGGGCCCTCTCC', 'rightprimer_start': '1165', 'rightprimer_tm': '60.9854895715', 'leftprimer_length': '20', 'template_length': 1169, 'leftprimer_gc': '60.0', 'leftprimer_start': '10', 'name': '2169235', 'guidecount': 21, 'leftprimer_tm': '60.6183932401', 'product_length': 1155, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCGGGGGTCTAGTGGGTTTA', 'expandedpriming': 20}
{'rightprimer': 'GAGGGACGGAGGAAGAGGAA', 'rightprimer_start': '1041', 'rightprimer_tm': '60.3244599053', 'leftprimer_length': '23', 'template_length': 1200, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '51', 'name': '2342276', 'guidecount': 21, 'leftprimer_tm': '60.307974677', 'product_length': 990, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'AGCTGCTGTATCAATTCCACAGT', 'expandedpriming': 50}
{'rightprimer': 'CCATGGCCAGGTAGTGAAGC', 'rightprimer_start': '905', 'rightprimer_tm': '60.7494544953', 'leftprimer_length': '20', 'template_length': 900, 'leftprimer_gc': '55.0', 'leftprimer_start': '60', 'name': '2430937', 'guidecount': 21, 'leftprimer_tm': '60.1791450119', 'product_length': 845, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GACAGATGCCTGCAACATGC', 'expandedpriming': 0}
{'rightprimer': 'ACACTTACCCGGGTAATTCTGC', 'rightprimer_start': '522', 'rightprimer_tm': '60.3554115786', 'leftprimer_length': '20', 'template_length': 515, 'leftprimer_gc': '55.0', 'leftprimer_start': '6', 'name': '2533870', 'guidecount': 21, 'leftprimer_tm': '59.9618343238', 'product_length': 516, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'TCCACCAGGTTCTCATTGCC', 'expandedpriming': 0}
{'rightprimer': 'CAGCCCCGGCCATGTCAC', 'rightprimer_start': '2579', 'rightprimer_tm': '63.475807045', 'leftprimer_length': '20', 'template_length': 2569, 'leftprimer_gc': '60.0', 'leftprimer_start': '1', 'name': '2616738', 'guidecount': 21, 'leftprimer_tm': '62.7696520845', 'product_length': 2578, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'TGCCTGGCTGAACATCACCC', 'expandedpriming': 0}
{'rightprimer': 'CATCCGGCCCACATACCAG', 'rightprimer_start': '1077', 'rightprimer_tm': '60.2279429682', 'leftprimer_length': '20', 'template_length': 1302, 'leftprimer_gc': '60.0', 'leftprimer_start': '17', 'name': '2842594', 'guidecount': 21, 'leftprimer_tm': '59.9663575388', 'product_length': 1060, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'GAGCTGGGGCCGAGTAATAC', 'expandedpriming': 20}
{'rightprimer': 'GCCGGATGAAAATGAATCCACA', 'rightprimer_start': '1375', 'rightprimer_tm': '59.5732622562', 'leftprimer_length': '18', 'template_length': 1376, 'leftprimer_gc': '72.2222222222', 'leftprimer_start': '6', 'name': '3495299', 'guidecount': 21, 'leftprimer_tm': '62.7854525363', 'product_length': 1369, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'CTGCCTTCCCTCCGCCAC', 'expandedpriming': 20}
{'rightprimer': 'GCCTTTCTGCTAGAGGCCTT', 'rightprimer_start': '1215', 'rightprimer_tm': '59.7455375053', 'leftprimer_length': '20', 'template_length': 1208, 'leftprimer_gc': '55.0', 'leftprimer_start': '9', 'name': '3875524', 'guidecount': 21, 'leftprimer_tm': '60.1789030603', 'product_length': 1206, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACTCCCAGCACTCCACCTAA', 'expandedpriming': 0}
{'rightprimer': 'GGGTATGTGAGTTCTCTAGGGC', 'rightprimer_start': '1206', 'rightprimer_tm': '59.6340589105', 'leftprimer_length': '20', 'template_length': 1204, 'leftprimer_gc': '60.0', 'leftprimer_start': '16', 'name': '4166851', 'guidecount': 21, 'leftprimer_tm': '59.5179937681', 'product_length': 1190, 'rightprimer_length': '22', 'rightprimer_gc': '54.5454545455', 'leftprimer': 'GGACCCCTACTCCCGTCTAT', 'expandedpriming': 20}
{'rightprimer': 'TAAAGCCCCCCTGGTGGAA', 'rightprimer_start': '1122', 'rightprimer_tm': '60.4663862112', 'leftprimer_length': '19', 'template_length': 1114, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '2', 'name': '4251495', 'guidecount': 21, 'leftprimer_tm': '60.4492463442', 'product_length': 1120, 'rightprimer_length': '19', 'rightprimer_gc': '57.8947368421', 'leftprimer': 'CAGGCAGTAGAGGCAGTGC', 'expandedpriming': 0}
{'rightprimer': 'GCATAAATACCTGATGGGGTGGA', 'rightprimer_start': '1185', 'rightprimer_tm': '60.1814336666', 'leftprimer_length': '19', 'template_length': 1205, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '25', 'name': '4395570', 'guidecount': 21, 'leftprimer_tm': '60.5388759241', 'product_length': 1160, 'rightprimer_length': '23', 'rightprimer_gc': '47.8260869565', 'leftprimer': 'CACTGGGGAAGGAGGGTCA', 'expandedpriming': 20}
{'rightprimer': 'GGATGGCTTGCACCACAAAG', 'rightprimer_start': '1828', 'rightprimer_tm': '60.0379747013', 'leftprimer_length': '23', 'template_length': 1868, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '23', 'name': '4496504', 'guidecount': 21, 'leftprimer_tm': '59.9948446993', 'product_length': 1805, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CTTTATATGCTTGCTCCTTGGCC', 'expandedpriming': 20}
{'rightprimer': 'TTGAAACTATGGTGGGAAGGCT', 'rightprimer_start': '1651', 'rightprimer_tm': '59.6214531969', 'leftprimer_length': '20', 'template_length': 1749, 'leftprimer_gc': '55.0', 'leftprimer_start': '9', 'name': '4792613', 'guidecount': 21, 'leftprimer_tm': '60.0332078652', 'product_length': 1642, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'GGGGCCAGTGGAATGATTGA', 'expandedpriming': 50}
{'rightprimer': 'CTTAACTGGAGGTTCGGGGG', 'rightprimer_start': '1829', 'rightprimer_tm': '59.7478029137', 'leftprimer_length': '20', 'template_length': 1861, 'leftprimer_gc': '55.0', 'leftprimer_start': '722', 'name': '4953408', 'guidecount': 21, 'leftprimer_tm': '59.8881682628', 'product_length': 1107, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'ATGCCCATATGGTCAGGCAG', 'expandedpriming': 50}
{'rightprimer': 'GATGGCTTGTTGGGGACTGA', 'rightprimer_start': '1228', 'rightprimer_tm': '59.9618343238', 'leftprimer_length': '19', 'template_length': 1256, 'leftprimer_gc': '57.8947368421', 'leftprimer_start': '18', 'name': '5365570', 'guidecount': 21, 'leftprimer_tm': '60.001268924', 'product_length': 1210, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GCCCTCGGCTTTTACCCTT', 'expandedpriming': 20}
{'rightprimer': 'CATTCCAGGCCCGCCCAC', 'rightprimer_start': '3208', 'rightprimer_tm': '63.4990603635', 'leftprimer_length': '20', 'template_length': 3205, 'leftprimer_gc': '50.0', 'leftprimer_start': '222', 'name': '5553976', 'guidecount': 21, 'leftprimer_tm': '60.2513413668', 'product_length': 2986, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'AGCAACGGGCCTCAGAAATT', 'expandedpriming': 0}
{'rightprimer': 'AGTAAGGACCCGCAGCTTTC', 'rightprimer_start': '970', 'rightprimer_tm': '60.0362939165', 'leftprimer_length': '21', 'template_length': 1021, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '4', 'name': '5777991', 'guidecount': 21, 'leftprimer_tm': '59.9270345645', 'product_length': 966, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'CAGCTTCACCTGGAAAGTCCT', 'expandedpriming': 0}
{'rightprimer': 'AGCCCAGTTTCAAATTTGGC', 'rightprimer_start': '1255', 'rightprimer_tm': '57.4509364285', 'leftprimer_length': '20', 'template_length': 2125, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '6004608', 'guidecount': 21, 'leftprimer_tm': '57.2298362353', 'product_length': 1255, 'rightprimer_length': '20', 'rightprimer_gc': '45.0', 'leftprimer': 'AGTGTCTCCTGGTAATGCAC', 'expandedpriming': 0}
{'rightprimer': 'CTGCACATAAGGCCTCCAGT', 'rightprimer_start': '1338', 'rightprimer_tm': '59.7458219694', 'leftprimer_length': '21', 'template_length': 1415, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '0', 'name': '6044005', 'guidecount': 21, 'leftprimer_tm': '59.7263935161', 'product_length': 1338, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGCACCTTCTAGCTCACAGTC', 'expandedpriming': 0}
{'rightprimer': 'AGGCCAAACATTGATCACAAGG', 'rightprimer_start': '2311', 'rightprimer_tm': '59.4359379464', 'leftprimer_length': '20', 'template_length': 2320, 'leftprimer_gc': '60.0', 'leftprimer_start': '1455', 'name': '6316920', 'guidecount': 21, 'leftprimer_tm': '59.5321955284', 'product_length': 856, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'CTTCTCCTAGGCTTCCCTGC', 'expandedpriming': 20}
{'rightprimer': 'GCAGGGCATATGCTTTGGAC', 'rightprimer_start': '2573', 'rightprimer_tm': '59.6131617145', 'leftprimer_length': '20', 'template_length': 2573, 'leftprimer_gc': '55.0', 'leftprimer_start': '54', 'name': '6666897', 'guidecount': 21, 'leftprimer_tm': '59.9616899326', 'product_length': 2519, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACCCTAGTGGAAGCTTTGGC', 'expandedpriming': 20}
{'rightprimer': 'GTGCCCTGGCTATTGTGCTA', 'rightprimer_start': '1087', 'rightprimer_tm': '60.1072142032', 'leftprimer_length': '18', 'template_length': 1131, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '22', 'name': '6727758', 'guidecount': 21, 'leftprimer_tm': '60.1242355164', 'product_length': 1065, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACGGGACCGAGCCTCTAG', 'expandedpriming': 20}
{'rightprimer': 'GCCCTAGAGATATGGGGGCA', 'rightprimer_start': '2562', 'rightprimer_tm': '60.5477122992', 'leftprimer_length': '20', 'template_length': 2545, 'leftprimer_gc': '55.0', 'leftprimer_start': '40', 'name': '8006355', 'guidecount': 21, 'leftprimer_tm': '60.3204209304', 'product_length': 2522, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'GACCTCACCAAAGCAGCGTA', 'expandedpriming': 20}
{'rightprimer': 'TCTTATTAACTGGTTCCAAGGTCA', 'rightprimer_start': '1715', 'rightprimer_tm': '57.557459432', 'leftprimer_length': '20', 'template_length': 1724, 'leftprimer_gc': '55.0', 'leftprimer_start': '54', 'name': '8370756', 'guidecount': 21, 'leftprimer_tm': '59.614248662', 'product_length': 1661, 'rightprimer_length': '24', 'rightprimer_gc': '37.5', 'leftprimer': 'CTAGCACCGACGATCACCAT', 'expandedpriming': 20}
{'rightprimer': 'AGCTTCCATACTAGTTTGTTGGA', 'rightprimer_start': '1354', 'rightprimer_tm': '57.5009960125', 'leftprimer_length': '20', 'template_length': 1346, 'leftprimer_gc': '50.0', 'leftprimer_start': '108', 'name': '8573433', 'guidecount': 21, 'leftprimer_tm': '57.5204340729', 'product_length': 1246, 'rightprimer_length': '23', 'rightprimer_gc': '39.1304347826', 'leftprimer': 'GCACACCCTTCAGAGAATCA', 'expandedpriming': 0}
{'rightprimer': 'AGCATAGCAGCACCTAAGGT', 'rightprimer_start': '1420', 'rightprimer_tm': '58.7922087357', 'leftprimer_length': '23', 'template_length': 1465, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '3', 'name': '8900391', 'guidecount': 21, 'leftprimer_tm': '58.7430797712', 'product_length': 1417, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GCTTTCTAGCTTGTGAATCTGGA', 'expandedpriming': 0}
{'rightprimer': 'GGATCCTGGGGCCATGTT', 'rightprimer_start': '1883', 'rightprimer_tm': '58.9810992649', 'leftprimer_length': '20', 'template_length': 1867, 'leftprimer_gc': '50.0', 'leftprimer_start': '346', 'name': '9256400', 'guidecount': 21, 'leftprimer_tm': '59.4692560988', 'product_length': 1537, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'ATTCTCTGCGCTTCCGTGTA', 'expandedpriming': 0}
{'rightprimer': 'CCCCATAGGCTGCACAGTAG', 'rightprimer_start': '1475', 'rightprimer_tm': '59.8925443107', 'leftprimer_length': '20', 'template_length': 1815, 'leftprimer_gc': '55.0', 'leftprimer_start': '6', 'name': '9406349', 'guidecount': 21, 'leftprimer_tm': '59.5245251573', 'product_length': 1469, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'TGCTAGGGCCCAATGAGATG', 'expandedpriming': 0}
{'rightprimer': 'TGCACAACCAGGGTCACAAT', 'rightprimer_start': '3289', 'rightprimer_tm': '60.1062679146', 'leftprimer_length': '22', 'template_length': 3368, 'leftprimer_gc': '50.0', 'leftprimer_start': '21', 'name': '9771252', 'guidecount': 21, 'leftprimer_tm': '59.8333357345', 'product_length': 3268, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'ACCAGGAACTCTCGATAACTGC', 'expandedpriming': 20}
{'rightprimer': 'GTGTGGGACATGCATCTCCC', 'rightprimer_start': '603', 'rightprimer_tm': '60.7494544953', 'leftprimer_length': '18', 'template_length': 611, 'leftprimer_gc': '66.6666666667', 'leftprimer_start': '3', 'name': '9865413', 'guidecount': 21, 'leftprimer_tm': '61.4150874406', 'product_length': 600, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'ATGGCCAGGACAGAGCCC', 'expandedpriming': 0}
{'rightprimer': 'GTCTGTGCCTTCCCTGGTAT', 'rightprimer_start': '1170', 'rightprimer_tm': '59.0875135479', 'leftprimer_length': '20', 'template_length': 1154, 'leftprimer_gc': '45.0', 'leftprimer_start': '165', 'name': '10320341', 'guidecount': 21, 'leftprimer_tm': '59.1044699215', 'product_length': 1005, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGGGTTGGCAAACGTTTGAA', 'expandedpriming': 0}
{'rightprimer': 'GGCAAATTGCTCCCAAGTGG', 'rightprimer_start': '2003', 'rightprimer_tm': '60.0370457062', 'leftprimer_length': '20', 'template_length': 2063, 'leftprimer_gc': '55.0', 'leftprimer_start': '593', 'name': '10566212', 'guidecount': 21, 'leftprimer_tm': '60.0354388631', 'product_length': 1410, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'ACCTATTCCTTGTGCCGGTG', 'expandedpriming': 50}
{'rightprimer': 'GCCGGTATCACTAGCAGGATC', 'rightprimer_start': '708', 'rightprimer_tm': '60.0674949165', 'leftprimer_length': '20', 'template_length': 709, 'leftprimer_gc': '55.0', 'leftprimer_start': '26', 'name': '10781830', 'guidecount': 21, 'leftprimer_tm': '59.6683839568', 'product_length': 682, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'AGGTGCCCATAACCTGTTCC', 'expandedpriming': 20}
{'rightprimer': 'GCCATTCGTTAACCACACGA', 'rightprimer_start': '1048', 'rightprimer_tm': '58.8500133945', 'leftprimer_length': '20', 'template_length': 1136, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '10800135', 'guidecount': 21, 'leftprimer_tm': '57.2105912836', 'product_length': 1048, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GCTCCTGGGATTGTACAACT', 'expandedpriming': 0}
{'rightprimer': 'GGGTAGGAAAAATCTAGCCCCTC', 'rightprimer_start': '1530', 'rightprimer_tm': '60.1814423507', 'leftprimer_length': '23', 'template_length': 1513, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '129', 'name': '10871040', 'guidecount': 21, 'leftprimer_tm': '60.0554575872', 'product_length': 1401, 'rightprimer_length': '23', 'rightprimer_gc': '52.1739130435', 'leftprimer': 'TCAGAAGAACAACCTGTGTGACA', 'expandedpriming': 0}
{'rightprimer': 'CCCCAGGGGCACACATTT', 'rightprimer_start': '846', 'rightprimer_tm': '59.8797258977', 'leftprimer_length': '23', 'template_length': 845, 'leftprimer_gc': '52.1739130435', 'leftprimer_start': '20', 'name': '11183356', 'guidecount': 21, 'leftprimer_tm': '59.8633743921', 'product_length': 826, 'rightprimer_length': '18', 'rightprimer_gc': '61.1111111111', 'leftprimer': 'CCAACTAGTAGAAGGGAATGGGG', 'expandedpriming': 20}
{'rightprimer': 'AGATTGGTATCTTAGTCCGGCT', 'rightprimer_start': '1001', 'rightprimer_tm': '58.4220808737', 'leftprimer_length': '20', 'template_length': 999, 'leftprimer_gc': '55.0', 'leftprimer_start': '40', 'name': '11194893', 'guidecount': 21, 'leftprimer_tm': '58.476242288', 'product_length': 961, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'CCTAGGCACCCGAATTATGC', 'expandedpriming': 20}
{'rightprimer': 'CTCCTTCCCCCAGGTCTCT', 'rightprimer_start': '4146', 'rightprimer_tm': '59.6058490068', 'leftprimer_length': '19', 'template_length': 4133, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '12', 'name': '11533718', 'guidecount': 21, 'leftprimer_tm': '59.6220055248', 'product_length': 4134, 'rightprimer_length': '19', 'rightprimer_gc': '63.1578947368', 'leftprimer': 'CAGCCAGGCTCATATCCCC', 'expandedpriming': 20}
{'rightprimer': 'ACTATTTCTGCTAGTAGGGATGAA', 'rightprimer_start': '2579', 'rightprimer_tm': '56.6878560712', 'leftprimer_length': '20', 'template_length': 2571, 'leftprimer_gc': '50.0', 'leftprimer_start': '158', 'name': '13882255', 'guidecount': 21, 'leftprimer_tm': '57.1722002954', 'product_length': 2421, 'rightprimer_length': '24', 'rightprimer_gc': '37.5', 'leftprimer': 'CTGTCACATGTCTCTGGTGT', 'expandedpriming': 0}
{'rightprimer': 'CAGACATTGCTCAGACATTGTCA', 'rightprimer_start': '2424', 'rightprimer_tm': '59.2503910747', 'leftprimer_length': '23', 'template_length': 2417, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '16', 'name': '14521910', 'guidecount': 21, 'leftprimer_tm': '59.4872762241', 'product_length': 2408, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'CCACCAAGAATCTCAAATTGCCT', 'expandedpriming': 20}
{'rightprimer': 'CTTCACCCACAATCTGCCCT', 'rightprimer_start': '722', 'rightprimer_tm': '59.9612556273', 'leftprimer_length': '20', 'template_length': 1398, 'leftprimer_gc': '55.0', 'leftprimer_start': '3', 'name': '15451483', 'guidecount': 21, 'leftprimer_tm': '59.7409352444', 'product_length': 719, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TCAGACCGGGATCTGCTACT', 'expandedpriming': 20}
{'rightprimer': 'TAGAAGGGCAAGAGGGAGGT', 'rightprimer_start': '2164', 'rightprimer_tm': '59.5821592885', 'leftprimer_length': '20', 'template_length': 2152, 'leftprimer_gc': '55.0', 'leftprimer_start': '167', 'name': '15574645', 'guidecount': 21, 'leftprimer_tm': '59.8219317468', 'product_length': 1997, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGCTTGGCAAGGACTCGATA', 'expandedpriming': 20}
{'rightprimer': 'TTATTGCACGTAACGTTCCC', 'rightprimer_start': '1929', 'rightprimer_tm': '56.4606519265', 'leftprimer_length': '21', 'template_length': 2040, 'leftprimer_gc': '42.8571428571', 'leftprimer_start': '41', 'name': '15925074', 'guidecount': 21, 'leftprimer_tm': '56.672110639', 'product_length': 1888, 'rightprimer_length': '20', 'rightprimer_gc': '45.0', 'leftprimer': 'TTCCCTTGAGTCTCGAAATCA', 'expandedpriming': 50}
{'rightprimer': 'GGGTTGTAATGGCGCATTGT', 'rightprimer_start': '1823', 'rightprimer_tm': '59.469711984', 'leftprimer_length': '24', 'template_length': 2373, 'leftprimer_gc': '41.6666666667', 'leftprimer_start': '8', 'name': '16301969', 'guidecount': 21, 'leftprimer_tm': '57.0143465736', 'product_length': 1815, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'CAGGTATCCTCTATCCTCTTTCAA', 'expandedpriming': 20}
{'rightprimer': 'CCTCTTTTTCTAGTACCAAGCCC', 'rightprimer_start': '2369', 'rightprimer_tm': '58.7373828122', 'leftprimer_length': '21', 'template_length': 2362, 'leftprimer_gc': '42.8571428571', 'leftprimer_start': '84', 'name': '16337503', 'guidecount': 21, 'leftprimer_tm': '58.7702081587', 'product_length': 2285, 'rightprimer_length': '23', 'rightprimer_gc': '47.8260869565', 'leftprimer': 'ACAGCCTCTCGAAATTTTGCA', 'expandedpriming': 0}
{'rightprimer': 'GCTTTCTAACCCTGGCAACC', 'rightprimer_start': '1393', 'rightprimer_tm': '59.1113811937', 'leftprimer_length': '23', 'template_length': 1462, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '73', 'name': '16711783', 'guidecount': 21, 'leftprimer_tm': '58.9627782638', 'product_length': 1320, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGATAAGCCACCTAGACATGGAA', 'expandedpriming': 50}
{'rightprimer': 'ACAGGCAGCTCAGTTCCTTC', 'rightprimer_start': '1009', 'rightprimer_tm': '59.963977835', 'leftprimer_length': '22', 'template_length': 1297, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '4', 'name': '17098548', 'guidecount': 21, 'leftprimer_tm': '59.4875242527', 'product_length': 1005, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'AAGACTAGGGGGTGTTTTGTGT', 'expandedpriming': 0}
{'rightprimer': 'CCGAATACCCACCCAGTTCC', 'rightprimer_start': '1533', 'rightprimer_tm': '60.107361497', 'leftprimer_length': '19', 'template_length': 1696, 'leftprimer_gc': '63.1578947368', 'leftprimer_start': '18', 'name': '17368225', 'guidecount': 21, 'leftprimer_tm': '60.2276743795', 'product_length': 1515, 'rightprimer_length': '20', 'rightprimer_gc': '60.0', 'leftprimer': 'CCTGCTTACCGGGATGCTC', 'expandedpriming': 20}
{'rightprimer': 'GTAGCGGCCTCTTTACCTGT', 'rightprimer_start': '2954', 'rightprimer_tm': '59.4642026471', 'leftprimer_length': '18', 'template_length': 3314, 'leftprimer_gc': '55.5555555556', 'leftprimer_start': '0', 'name': '17600352', 'guidecount': 21, 'leftprimer_tm': '58.1663468641', 'product_length': 2954, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'TGCAGGGCCTGGAATGTA', 'expandedpriming': 0}
{'rightprimer': 'CTCCTCCCTGGCCAGTCT', 'rightprimer_start': '1494', 'rightprimer_tm': '59.9597222444', 'leftprimer_length': '20', 'template_length': 1480, 'leftprimer_gc': '55.0', 'leftprimer_start': '88', 'name': '18066788', 'guidecount': 21, 'leftprimer_tm': '60.1067190722', 'product_length': 1406, 'rightprimer_length': '18', 'rightprimer_gc': '66.6666666667', 'leftprimer': 'GGTTTAGGCCCAGCTATGCA', 'expandedpriming': 0}
{'rightprimer': 'CTAGAGCCTGGGGAGGGTAG', 'rightprimer_start': '2237', 'rightprimer_tm': '60.1789467508', 'leftprimer_length': '22', 'template_length': 2237, 'leftprimer_gc': '45.4545454545', 'leftprimer_start': '11', 'name': '18164577', 'guidecount': 21, 'leftprimer_tm': '59.9670761763', 'product_length': 2226, 'rightprimer_length': '20', 'rightprimer_gc': '65.0', 'leftprimer': 'GCAACACTGTTCTTAACGGCTT', 'expandedpriming': 20}
{'rightprimer': 'ATGGCGCAGGAAGTAAAGCA', 'rightprimer_start': '2308', 'rightprimer_tm': '60.3224559824', 'leftprimer_length': '23', 'template_length': 2673, 'leftprimer_gc': '47.8260869565', 'leftprimer_start': '3', 'name': '18313420', 'guidecount': 21, 'leftprimer_tm': '60.3067798136', 'product_length': 2305, 'rightprimer_length': '20', 'rightprimer_gc': '50.0', 'leftprimer': 'GGTAACTAGAGAAGCCAGTTGCA', 'expandedpriming': 0}
{'rightprimer': 'GGGGTTGGGCCAAGCACC', 'rightprimer_start': '1507', 'rightprimer_tm': '63.6998830164', 'leftprimer_length': '20', 'template_length': 1501, 'leftprimer_gc': '50.0', 'leftprimer_start': '0', 'name': '19235353', 'guidecount': 21, 'leftprimer_tm': '60.3227258871', 'product_length': 1507, 'rightprimer_length': '18', 'rightprimer_gc': '72.2222222222', 'leftprimer': 'TGCAAATGCTAGCTGCCTGA', 'expandedpriming': 0}
{'rightprimer': 'GCTAGTAGGCAATCTGTGGTCT', 'rightprimer_start': '1506', 'rightprimer_tm': '59.5674902756', 'leftprimer_length': '20', 'template_length': 1507, 'leftprimer_gc': '55.0', 'leftprimer_start': '29', 'name': '19882938', 'guidecount': 21, 'leftprimer_tm': '60.2512971105', 'product_length': 1477, 'rightprimer_length': '22', 'rightprimer_gc': '50.0', 'leftprimer': 'CCCATGGCTGGACTTTCACA', 'expandedpriming': 20}
{'rightprimer': 'CCTAGCAAAAGCTAACTGCCAAT', 'rightprimer_start': '1633', 'rightprimer_tm': '59.5549043236', 'leftprimer_length': '23', 'template_length': 1617, 'leftprimer_gc': '43.4782608696', 'leftprimer_start': '107', 'name': '19891785', 'guidecount': 21, 'leftprimer_tm': '59.8660987196', 'product_length': 1526, 'rightprimer_length': '23', 'rightprimer_gc': '43.4782608696', 'leftprimer': 'AGTGTGCCGGTGAATATGTATCA', 'expandedpriming': 20}
{'rightprimer': 'AGAGATGCTGTAACTGCTGCT', 'rightprimer_start': '2168', 'rightprimer_tm': '59.445839952', 'leftprimer_length': '21', 'template_length': 2219, 'leftprimer_gc': '52.380952381', 'leftprimer_start': '4', 'name': '19895054', 'guidecount': 21, 'leftprimer_tm': '59.9260765809', 'product_length': 2164, 'rightprimer_length': '21', 'rightprimer_gc': '47.619047619', 'leftprimer': 'CTGCCAGGTTACCTTGTTCCT', 'expandedpriming': 0}
{'rightprimer': 'AGGCAGTAAGTCTTTGGAACCA', 'rightprimer_start': '1265', 'rightprimer_tm': '59.5582496232', 'leftprimer_length': '20', 'template_length': 1309, 'leftprimer_gc': '55.0', 'leftprimer_start': '25', 'name': '21283043', 'guidecount': 21, 'leftprimer_tm': '59.6910069716', 'product_length': 1240, 'rightprimer_length': '22', 'rightprimer_gc': '45.4545454545', 'leftprimer': 'GCCCTCATGCGCTTCATTAC', 'expandedpriming': 50}
{'rightprimer': 'AATCCGGGGGATGGAAGTCT', 'rightprimer_start': '2428', 'rightprimer_tm': '60.3275764218', 'leftprimer_length': '20', 'template_length': 2427, 'leftprimer_gc': '55.0', 'leftprimer_start': '633', 'name': '21669639', 'guidecount': 21, 'leftprimer_tm': '60.1790176843', 'product_length': 1795, 'rightprimer_length': '20', 'rightprimer_gc': '55.0', 'leftprimer': 'GGAACACAGCCAGGACACTT', 'expandedpriming': 20}
{'rightprimer': 'GCTCCCACTCCAGGATAAAGC', 'rightprimer_start': '2140', 'rightprimer_tm': '60.4759304564', 'leftprimer_length': '20', 'template_length': 2132, 'leftprimer_gc': '55.0', 'leftprimer_start': '14', 'name': '21900290', 'guidecount': 21, 'leftprimer_tm': '59.7458219694', 'product_length': 2126, 'rightprimer_length': '21', 'rightprimer_gc': '57.1428571429', 'leftprimer': 'GGAGTCTGATGGCCACACAT', 'expandedpriming': 0}
In [148]:
goodruns_filtered[3].permissible_region
Out[148]:
SeqRecord(seq=Seq('aaccagatagctgctgaaactccaaactggacagctgctgaacaaaaagtgaac...GGA', IUPACAmbiguousDNA()), id='17467976', name='17467976', description='59', dbxrefs=[])
In [149]:
for item in goodruns_filtered_with_primers:
starts = [int(g.name) for g in item.guides]
startBinValue = int(item.guides[0].name)
endBinValue = int(item.guides[-1].name)
bins = np.arange(startBinValue, endBinValue, 22)
starts_binned = np.digitize(starts, bins)
item.guidecount_nooverlap = len(set(starts_binned))
In [150]:
len(goodruns_filtered_with_primers)
Out[150]:
642
In [151]:
noexp = sorted([item for item in goodruns_filtered_with_primers if item.primers["expandedpriming"] <1], reverse=True, key=attrgetter("guidecount"))[:432]
exp20 = sorted([item for item in goodruns_filtered_with_primers if item.primers["expandedpriming"] <=20], reverse=True, key=attrgetter("guidecount"))[:432]
exp50 = sorted([item for item in goodruns_filtered_with_primers if item.primers["expandedpriming"] <=50], reverse=True, key=attrgetter("guidecount"))[0:432]
exp50_nooverlap = sorted([item for item in goodruns_filtered_with_primers if item.primers["expandedpriming"] <=50], reverse=True, key=attrgetter("guidecount_nooverlap"))[0:432]
In [152]:
print(len(noexp), sum([item.guidecount for item in noexp]))
print(len(exp20), sum([item.guidecount for item in exp20]))
print(len(exp50), sum([item.guidecount for item in exp50]))
print(len(exp50), sum([item.guidecount_nooverlap for item in exp50]))
print(len(exp50), sum([item.guidecount_nooverlap for item in exp50_nooverlap]))
(323, 8690)
(432, 12384)
(432, 12721)
(432, 10171)
(432, 10444)
In [153]:
len(list(al_digesttarget([item.permissible_region_masked[int(item.primers["leftprimer_start"]):\
int(item.primers["rightprimer_start"])]\
for item in exp50_nooverlap[0:432]])))
Out[153]:
12208
In [154]:
len(list(al_digesttarget(\
[item.permissible_region_masked for item in exp50_nooverlap])))
Out[154]:
12626
In [155]:
len(list(al_digesttarget(\
[item.permissible_region_masked for item in exp20])))
Out[155]:
12582
In [156]:
len(list(al_digesttarget(\
[item.permissible_region for item in noexp])))
Out[156]:
8968
In [254]:
exp50[0].primers
Out[254]:
{'expandedpriming': 20,
'guidecount': 65,
'leftprimer': 'TAGAGGCCCTCAGGATAGCC',
'leftprimer_gc': '60.0',
'leftprimer_length': '20',
'leftprimer_start': '9',
'leftprimer_tm': '59.8846505619',
'name': '31146560',
'product_length': 5643,
'rightprimer': 'ACAGTTCTAGTTGGTTGGCCA',
'rightprimer_gc': '47.619047619',
'rightprimer_length': '21',
'rightprimer_start': '5652',
'rightprimer_tm': '59.506977668',
'template_length': 5782}
In [193]:
sum([(int(item.primers["leftprimer_length"]) + int(item.primers["rightprimer_length"])) for item in exp50_nooverlap[0:432]])
Out[193]:
17655
In [97]:
goodguides = []
badguides = []
for item in al_digesttarget([item.permissible_region for item in exp50]):
if al_scoreguide(item, genomename, genomedict) < 94:
badguides.append(item)
else:
goodguides.append(item)
In [31]:
len(goodguides)
---------------------------------------------------------------------------
NameError Traceback (most recent call last)
<ipython-input-31-c0d6cad3e0f8> in <module>()
----> 1 len(goodguides)
NameError: name 'goodguides' is not defined
In [32]:
len(badguides)
---------------------------------------------------------------------------
NameError Traceback (most recent call last)
<ipython-input-32-90b0dd76eb7e> in <module>()
----> 1 len(badguides)
NameError: name 'badguides' is not defined
In [115]:
dir(exp50[0])
Out[115]:
['__doc__',
'__init__',
'__module__',
'chromosome',
'end',
'fiveprimeabut',
'genomename',
'guidecount',
'guides',
'left_inside',
'left_outside',
'length',
'permissible_end',
'permissible_region',
'permissible_start',
'primers',
'pullsequences',
'required_end_absolute',
'required_end_relative',
'required_start_absolute',
'required_start_relative',
'right_inside',
'right_outside',
'start',
'threeprimeabut']
In [33]:
exp50[0].primers
Out[33]:
{'expandedpriming': 20,
'guidecount': 65,
'leftprimer': 'TAGAGGCCCTCAGGATAGCC',
'leftprimer_gc': '60.0',
'leftprimer_length': '20',
'leftprimer_start': '9',
'leftprimer_tm': '59.8846505619',
'name': '31146560',
'product_length': 5643,
'rightprimer': 'ACAGTTCTAGTTGGTTGGCCA',
'rightprimer_gc': '47.619047619',
'rightprimer_length': '21',
'rightprimer_start': '5652',
'rightprimer_tm': '59.506977668',
'template_length': 5782}
In [192]:
print exp50[0].permissible_region.seq
CTGTCTAACTAGAGGCCCTCAGGATAGCCAAGTTCACCATAGACCTCCATGTATGACACTGTTGTATAATAGTCTTCCCCCCATATATGTTTAATGGGGGCAACTCTTCATGTATGTTGAAGGACAAACACCATATAGCCCATGGTAGGAAGTAACCATTTTTTCTTACCATAGTTGTGTAGTTATCATACAACGTCTTGTTAAAATCCAATGTACAATTAAAATACTTTGTTCACTTTGTGGATAACTGGCCATAGCTTGAGGTAACACTGGGCCCAGATATCAGGATAAAATCAGAAAGGGCTCAGAGGCAATCAGGCCTCAGCTGAATGGGTATTGAGCCTGGGATCACATGCAGATCCCCTCAGGTCAACTACATTGACTTTCAGAGATAAGGCTCAGCGGGATGCAGCTTTGGACATAATCATGGAAATCCTAGCTTTTTTAACACTTATCCCCAAAGCCTGGGTAACTGAAATAACAGACAAGAATATGTACGGTCTAATCCTCTGAAAGAACATTCACCGTGGTAAAGATTTGTTGACAGGGCTCACTGGGGCTGCAGCGAACATCTCATTGCTATTGTTCACGTCAAGCTGTACTTTTCCAGCTTAATCTGGAACAAAGGGTAATGCGGGTTAACTATTAAGGCAAATGGTATTAGTTTCACCCTTCAAAAGTTTTATACTTAGAAATTTCATACTTAGAAATGTCACGTGCTGTTGACATGTTGACAGGTCCAGGAAGCCATTTTTATTTGGTCCCAATCTGTGACATTAAGTACTTTTCAACCAAAGCAGTCCACCATATAATACGTATATTTTATCAGCAGGCTTAGTGGAAAATCCACTGGTAAAGTATAGTTCATTTAAACCCTTACATATTCCTGGATATAAAATTAAGAACGTCCTCCAACTTTAAAAAGATGTGATAGCTTTTTTTGCTGAGTTGAAGGAAAAGATTTGATGTCTGAGATGGTAATGGAATCACAGTTACAGCACAAAGGATGCTGGGAGGCTCCCAAAATGCTGTATCTAGGATTTATTACATAGGGTGATATGGGGACTTGGTCGTTTTCTAATATGCACACTTTAAAACTTATTTTCCTTTTCCCCCATCTTATATAAAGTCTGTAACGGCATGTCACCTTCTGAACTGTTACATTGTGATGCATTAGTCGAAACTCTTCTCAGCAGAGTCACAGAAATGCTGTAACAGTTTGGAAAGCCAGAGTTCCTATGCTAGTTATTAAGGCTATTAACAAACACAAATATTTGTGTCTCCAATATCTGTTAATGTGGGAAAGGTATCTACAGTATAGTTAAAAACATTTGCACTTTGCACTTTATTAAATGAGCCCAATGTACTGGAGTCATCCCCACTGTCTCTCTACCATAGAGTTCTATAGCAGCTCAGATTGGCCTATTTGTACTTTCTCTATGCTACCTTTGTTACTTTTGCCATTCTTAGTATCCTAAGCTTTTTTATTGCCTTATTATATTACTTCTTCTTAATCTGTTGATATATTTTGTGTCAGCTTATTCACACACCTGTATATAGGAACTTCAAGTATGCAAAGGAAGAATGTCTGCACCCATCCCATAACCATCTATTCAAGTGGACAGAGATGAAAGATCAGCCTTACTAATATGGGGGGTATCATTTCTCTTTCTTTTTCAGGTATTCAGTACTTCCTAAATAAACTCAATTCTCTGGTTGTCACTTATCTAAATACCATTTTCCAGGGATGGGAAGGAGGCACTGAGGCATTCTAGATAAAATAGAGATGGTCCTAACATGCCACTGACCCGTCATTGGTATGCAGAGTCGTATAACTTCTAATGACAAGATAGAGACACAAAGGCTTAATGTAGGGAAGATGTAGAAGATATTCAGTGTACTTATCTCCCAGGTGTAATTATGCAGGAATTTTCAGCAAAACAATTCATTTTGGTAAGAAACTATAAAGCATTGTGTTGCTTTTTAAAGGTTTTTGCTCTCTGACTCCAATCTTAGAAATTAGATTTTTAAAATTGCATGGCGAATGCAAACCAATCTCTTGCGAGCATGGCATCATACCACGTATTCATAAATATGAATAAATTCCCGGCATGGCCACAACAAACCCCATTGTAAGCCTGGTGGTGTATTTACAACATGCTTTGTACAGCCACCCCCTGCAGGAGCACCCACTGTCTGATTGGCAGACACATTGTGTGTACATTTTCTTATGTAGCAAAGATGTCAATGTATGCAGTGAAGAACATACATTATAGCCCCTACATTCTACTCAGTGTATTTTTGGGGGCTACACactccactcaacttcaactccagactcaactGCCCCCTATATACAACTTATCCCCAACATATTATTAATGATCATTTCTGCCCAAAAGTCTAAAGCCCAAGGTGGGACAAGGATTTTAAAATGAATTCTCTATTAGGAAATACAAGGTAGGAGATCCATCACATGTGGTATTTACAGTATATCCCTTAAGGCAGAGCTGTTTATCTTAAATACCTTATACATCTGGGCAGATTACAACATAAGCATCTCATATTGGGGTGGAGATATTGGTAACCCTGTTGCTAGGCAACCAGGATTGCATGTCAGTGGATCATAAAAGATTCTTAGTGCCACATAGGAAGAATTCATTATTCCTATTAAAATCACAGATCTGCTCCATCTATATGCCTTGCCTACCTCTTCAGAAACTGCACAGTATATAATATAATAATCTGATGGTTAATCGTATGCATTAACATTGTTGTGTTGAAGGTAAAAAATAGTCTGTGCTATTTTAATGTTCCCAGCCTAGGCCTGAGCTGTTACATACATTACGGAAAAGTTATATTTCTGGTCAATAATTCCCAAGACAAGGCTTAAGGATGTGTATATGTGGACTTGTCTGGCTTTCAGGAACGTGCACAATTATACTGCTACAAGCTGAGCCACACTACTTATTGTAAGAGTGACATTGCCACCCAACAAGCTCTGCTATAAAAGAATGAATTCAGAGAGACAGCTCTCCAATATTCAGCTTCTGATGATATTAGACATGATTGTCTGCTCCAGTCTATGGCAATGAACATTAGCTACCCTCTCAAGGTCACCGGCAAGGACATAGCAGGAAGAGAGGAGTGAGCCATTGATTTTGCAGTAACAAGGTTTACATTATGGCAGATGGAAACCAGATTTTGATTTGAAGGGCATAAAGGGAGCGCCACCCTCCCACAGAGGAGGGTAGGATCATGCCCAGGTCTGAGCAGCAGCTGCCGGTTTAACCACCCCTATGCCAAACTCTTTGCAAAATAAAACGGTAACAAGCTAACATTTTACTTTATAGAAAGCCAACAGCAACAAAAGTTACATACTAATGtaattataataattgtaatcattaaatataattataatGCATTCAGATCAATTATAAGAAATATTTATGTTGTCTAATAATAGATAAAAAAGGTAAAGTTAAAAGAAGCTTTCATGTCATGCTGTATAAACTAGCTGAAGGGGCTATTAGGGATTAGAGTTACAGGGAGAGTTTACATAAAGCAATCATTGGATTACatgaagcagtacaaggaaatataggggagggttatatggagcaataggaggagttatagggagggttatatggagtaataggaggagttatagggagggttatatggagcaataggaggagttatagggagggttatatggagcaataggaggagttatagggagggttatatggagccaataggaggagttatagggaaggctatatggagcaataggaggagttatagggagagttatatggagcaataagaggggttatagggagggttatatggagcaatgggaggatttatagggagggccatatggagcaataggaggagttatagggagggttatatggagcaataggaggagttatagggagggttatatggagcaataggtggagttatagggagggttatatggagcaataggaggagttatagggagggttatatggagcaataggaggggttatagggagggttatatggagtaataggaggagttatagggagggttatatggagcaatagaaggagttatagggaggattatatggagcaataggaggagttatagggagggttatatggagcaataggaggaggtattggaagggttatatggaggaacaagtgttataagaaCTAATAGAATAAATAATAGGGGTCAGTTATAGGCAAAATGGGAGGTATAGATACCGGGTAATAAAGAAGTCCATCTTCCCTAATGACGGCTTATAAGGAGTTTAATAAAAATACTTCAGGGTATTACTAATGAGAATACATAACAAAGACATACAATTTATGTACAATTGCTTTGTACTTACTGTCAAGGCAAGGTTCGCAAACAGTTTGGTTTACTCCACAGGGCTTCATAACCCCCTCGCCTGCTGGGCAAGAAATACAACATTCCCCTTTTGCTGTGTATTTCTCTGACCAACATGGCTCCTCCTCAGCATTCAGCAATGTCTGCAAGAGAACACAACAGGTTAGATTCTGGAAAAGTAGAACTCAGCGAATTAGCTGGCAAAAACTACAGGCCAAAAACAAAAGAAAGAGCCTACTGGTAGTGAACAGGGCATAAATATGGGAACATGAAGTGGTAGAGTTGGGTTTGAAGGTCAGATATAGAAAAACATACAGATGGAAATTATCATTTCATCTATCTCTACACAGGTTTACCTGGTAAGTGAAACATGTTTTAGAAGAGACCAAGATAGGAGGAAAGATGAGTGAGAATCTGCAATGGAAGTGATAAACTGGTCAGAGAAGTAAGAAGATGGTAAGAGAAGTCAGTGGGCAAAGTCATCATAAGAAGAGAATCTGGTAGGAAGGGCCAATGAGAAAATTGAAGGAGACGTCATTTAGAACCTATTAGGATAAGTCAGTGGGAACCTAGTAGGACAGGTCAGTTAGAACCTAGTATGAAAAGAAATATAGTAAGAAAGGTCAGTGGGAATTTGGTGGGAGAGGTAAGATAATTTCTAATCTGATAAAAGAAGTGAATAAACATTTGATATGGAAGACCAGTGAGAATTTGGTAATAGGAATCTGCTAGTTAAGGGTAATAAAAATTCCACAGGACAGCGTAGCATGAATTCAGTGAGAAGGGTCTGTGAATTCGGTGAGGACATGTTGACAGACAATAACTAGTTGGATATTTTGTCCCTGTGCCATACAATTCTAAGCATGACAGATCATTCCAGTAAGAGAGAGCATCAAACTGAGCACGTAATGAACCTTCCTTGTATAATAGTTCACTAGTTAAATAAATCAAACTGTCACAGCTAACCCAGTGGGGGCGGGGGCATGGGTTTGCAAATTTATCAAAGCTTGTTTCCCTGTTCTAGTAAACGTATGTCCGGGTCATCACGGTTTGTTAGGGACATTAATCTGAAGCATTTGTATTGTAAAATAAAGATTAGTCACAATTCAGCACGGGGATGCCTGACATCTGTTACAATTCCTGATTGCGTACAGTACTGCTACTACACGGGGATCCAGCCAGCTTCATTCATTGTGAAACACATAAACATAAACCCAAACATTCACCGCTTGGCCAACCAACTAGAACTGTCATTGTACAATGGATAGAGcagtgtttttcaacctttttttggcaaaggcacacttgtttcatgaaaaaaatcacgaggcacaccaccattagaaaatgttaaaaaatttaactctgtgcccagcagcagtgcccccc
In [180]:
exp50bylen = sorted(exp50_nooverlap, key=lambda a: a.primers["product_length"], reverse=True)
In [181]:
len(exp50_nooverlap)
Out[181]:
432
In [182]:
exp50bylen[0].guidecount
Out[182]:
26
In [184]:
zip([(item.primers["product_length"], item.guidecount_nooverlap, index) for index, item in enumerate(exp50bylen)], [(item.primers["product_length"], index) for index, item in enumerate(exp50_nooverlap)])
Out[184]:
[((6133, 22, 0), (4766, 0)),
((6105, 28, 1), (5895, 1)),
((5895, 54, 2), (5643, 2)),
((5698, 40, 3), (3693, 3)),
((5686, 20, 4), (5146, 4)),
((5643, 51, 5), (4438, 5)),
((5146, 44, 6), (3087, 6)),
((5045, 33, 7), (3230, 7)),
((4941, 38, 8), (4666, 8)),
((4838, 35, 9), (2395, 9)),
((4766, 54, 10), (5698, 10)),
((4700, 29, 11), (3077, 11)),
((4693, 28, 12), (3081, 12)),
((4666, 41, 13), (2200, 13)),
((4438, 42, 14), (3286, 14)),
((4306, 34, 15), (4941, 15)),
((4269, 23, 16), (2909, 16)),
((4262, 21, 17), (2816, 17)),
((4232, 28, 18), (3263, 18)),
((4229, 29, 19), (3049, 19)),
((4097, 25, 20), (4838, 20)),
((4065, 33, 21), (3372, 21)),
((3996, 30, 22), (2719, 22)),
((3978, 31, 23), (3622, 23)),
((3930, 27, 24), (2697, 24)),
((3921, 33, 25), (3319, 25)),
((3905, 22, 26), (3374, 26)),
((3900, 33, 27), (4306, 27)),
((3881, 22, 28), (2359, 28)),
((3822, 23, 29), (3921, 29)),
((3812, 24, 30), (2945, 30)),
((3803, 28, 31), (5045, 31)),
((3779, 26, 32), (3169, 32)),
((3720, 23, 33), (4065, 33)),
((3710, 27, 34), (1845, 34)),
((3693, 47, 35), (3212, 35)),
((3667, 19, 36), (3900, 36)),
((3666, 20, 37), (3447, 37)),
((3658, 19, 38), (2222, 38)),
((3645, 28, 39), (3010, 39)),
((3626, 30, 40), (2645, 40)),
((3622, 34, 41), (2664, 41)),
((3568, 21, 42), (2648, 42)),
((3563, 21, 43), (2240, 43)),
((3515, 20, 44), (2589, 44)),
((3511, 25, 45), (2508, 45)),
((3447, 33, 46), (3047, 46)),
((3445, 20, 47), (1746, 47)),
((3410, 26, 48), (2680, 48)),
((3394, 23, 49), (2862, 49)),
((3392, 20, 50), (3978, 50)),
((3389, 20, 51), (2986, 51)),
((3387, 28, 52), (3223, 52)),
((3387, 23, 53), (2382, 53)),
((3377, 21, 54), (2828, 54)),
((3374, 34, 55), (2961, 55)),
((3372, 34, 56), (3131, 56)),
((3363, 23, 57), (2806, 57)),
((3359, 25, 58), (3996, 58)),
((3357, 25, 59), (2557, 59)),
((3351, 23, 60), (3626, 60)),
((3337, 22, 61), (4229, 61)),
((3319, 34, 62), (2653, 62)),
((3294, 28, 63), (3213, 63)),
((3286, 39, 64), (2007, 64)),
((3265, 24, 65), (4700, 65)),
((3263, 35, 66), (1322, 66)),
((3257, 21, 67), (2522, 67)),
((3252, 23, 68), (4232, 68)),
((3252, 19, 69), (2247, 69)),
((3239, 26, 70), (3803, 70)),
((3230, 41, 71), (2547, 71)),
((3227, 27, 72), (3645, 72)),
((3223, 31, 73), (1877, 73)),
((3222, 24, 74), (3387, 74)),
((3221, 24, 75), (4693, 75)),
((3213, 29, 76), (3294, 76)),
((3212, 33, 77), (6105, 77)),
((3209, 25, 78), (2246, 78)),
((3202, 26, 79), (2940, 79)),
((3188, 24, 80), (3227, 80)),
((3169, 33, 81), (2831, 81)),
((3167, 23, 82), (2013, 82)),
((3163, 21, 83), (2356, 83)),
((3160, 20, 84), (2849, 84)),
((3131, 30, 85), (2351, 85)),
((3130, 24, 86), (3930, 86)),
((3109, 25, 87), (1806, 87)),
((3106, 20, 88), (3089, 88)),
((3089, 27, 89), (2936, 89)),
((3087, 42, 90), (1748, 90)),
((3081, 39, 91), (3710, 91)),
((3077, 39, 92), (1650, 92)),
((3064, 20, 93), (1811, 93)),
((3049, 35, 94), (1831, 94)),
((3047, 31, 95), (3779, 95)),
((3044, 22, 96), (2287, 96)),
((3027, 20, 97), (2746, 97)),
((3010, 32, 98), (3410, 98)),
((3002, 21, 99), (2107, 99)),
((3001, 23, 100), (2160, 100)),
((2986, 31, 101), (1919, 101)),
((2981, 23, 102), (3239, 102)),
((2980, 21, 103), (2482, 103)),
((2961, 30, 104), (2336, 104)),
((2954, 20, 105), (2136, 105)),
((2945, 33, 106), (2423, 106)),
((2940, 27, 107), (3202, 107)),
((2936, 27, 108), (1455, 108)),
((2936, 23, 109), (2450, 109)),
((2935, 23, 110), (2172, 110)),
((2933, 20, 111), (1525, 111)),
((2909, 36, 112), (1641, 112)),
((2907, 22, 113), (2433, 113)),
((2903, 22, 114), (2414, 114)),
((2891, 21, 115), (2030, 115)),
((2884, 21, 116), (2378, 116)),
((2880, 19, 117), (1971, 117)),
((2871, 24, 118), (1950, 118)),
((2862, 31, 119), (2156, 119)),
((2862, 24, 120), (1636, 120)),
((2852, 21, 121), (3109, 121)),
((2849, 27, 122), (2246, 122)),
((2831, 27, 123), (4097, 123)),
((2828, 30, 124), (1782, 124)),
((2828, 20, 125), (2333, 125)),
((2816, 35, 126), (1544, 126)),
((2812, 24, 127), (3209, 127)),
((2806, 30, 128), (3359, 128)),
((2806, 23, 129), (2114, 129)),
((2802, 24, 130), (2642, 130)),
((2799, 20, 131), (3511, 131)),
((2766, 24, 132), (2208, 132)),
((2760, 22, 133), (1915, 133)),
((2758, 24, 134), (2377, 134)),
((2746, 26, 135), (2353, 135)),
((2744, 21, 136), (2530, 136)),
((2739, 21, 137), (2529, 137)),
((2725, 24, 138), (3357, 138)),
((2719, 34, 139), (1457, 139)),
((2712, 22, 140), (1277, 140)),
((2697, 34, 141), (2205, 141)),
((2686, 24, 142), (1685, 142)),
((2682, 23, 143), (1101, 143)),
((2680, 31, 144), (1935, 144)),
((2664, 32, 145), (1449, 145)),
((2659, 20, 146), (1277, 146)),
((2657, 23, 147), (2219, 147)),
((2653, 29, 148), (1549, 148)),
((2652, 22, 149), (2725, 149)),
((2648, 32, 150), (2812, 150)),
((2645, 32, 151), (2216, 151)),
((2643, 21, 152), (2236, 152)),
((2642, 25, 153), (2766, 153)),
((2634, 19, 154), (1465, 154)),
((2632, 19, 155), (2862, 155)),
((2611, 20, 156), (2871, 156)),
((2599, 20, 157), (3265, 157)),
((2592, 23, 158), (2758, 158)),
((2589, 31, 159), (2238, 159)),
((2577, 24, 160), (3222, 160)),
((2572, 21, 161), (2686, 161)),
((2562, 23, 162), (1695, 162)),
((2560, 20, 163), (2802, 163)),
((2557, 30, 164), (1248, 164)),
((2549, 23, 165), (3812, 165)),
((2547, 28, 166), (3130, 166)),
((2542, 21, 167), (2106, 167)),
((2530, 25, 168), (2278, 168)),
((2530, 23, 169), (2464, 169)),
((2529, 25, 170), (2042, 170)),
((2522, 28, 171), (2577, 171)),
((2508, 31, 172), (3221, 172)),
((2501, 20, 173), (2455, 173)),
((2482, 26, 174), (3188, 174)),
((2472, 20, 175), (1508, 175)),
((2471, 21, 176), (2592, 176)),
((2464, 24, 177), (1099, 177)),
((2460, 19, 178), (1575, 178)),
((2455, 24, 179), (1168, 179)),
((2452, 20, 180), (1819, 180)),
((2450, 26, 181), (2936, 181)),
((2439, 20, 182), (1184, 182)),
((2438, 21, 183), (1578, 183)),
((2433, 26, 184), (2191, 184)),
((2425, 21, 185), (1474, 185)),
((2423, 26, 186), (3394, 186)),
((2416, 23, 187), (3167, 187)),
((2414, 26, 188), (1713, 188)),
((2407, 22, 189), (2343, 189)),
((2405, 22, 190), (1577, 190)),
((2395, 40, 191), (2806, 191)),
((2382, 31, 192), (1461, 192)),
((2378, 25, 193), (2549, 193)),
((2377, 25, 194), (1869, 194)),
((2370, 23, 195), (3001, 195)),
((2367, 23, 196), (2416, 196)),
((2367, 22, 197), (2682, 197)),
((2359, 33, 198), (1679, 198)),
((2356, 27, 199), (2935, 199)),
((2353, 25, 200), (2367, 200)),
((2352, 22, 201), (3387, 201)),
((2351, 27, 202), (3822, 202)),
((2350, 20, 203), (1846, 203)),
((2343, 23, 204), (2981, 204)),
((2336, 26, 205), (3720, 205)),
((2334, 22, 206), (3363, 206)),
((2333, 25, 207), (2022, 207)),
((2325, 21, 208), (2657, 208)),
((2307, 21, 209), (2530, 209)),
((2287, 26, 210), (2370, 210)),
((2287, 21, 211), (3252, 211)),
((2284, 19, 212), (2038, 212)),
((2279, 20, 213), (2037, 213)),
((2278, 24, 214), (4269, 214)),
((2262, 22, 215), (2023, 215)),
((2261, 22, 216), (2562, 216)),
((2252, 22, 217), (3351, 217)),
((2247, 28, 218), (2652, 218)),
((2246, 28, 219), (1471, 219)),
((2246, 25, 220), (2903, 220)),
((2240, 32, 221), (918, 221)),
((2238, 24, 222), (1506, 222)),
((2236, 24, 223), (3337, 223)),
((2235, 20, 224), (1535, 224)),
((2231, 22, 225), (1354, 225)),
((2226, 20, 226), (2367, 226)),
((2223, 21, 227), (2405, 227)),
((2222, 32, 228), (2334, 228)),
((2219, 24, 229), (1429, 229)),
((2219, 20, 230), (6133, 230)),
((2218, 21, 231), (1763, 231)),
((2216, 24, 232), (3881, 232)),
((2208, 25, 233), (1939, 233)),
((2205, 24, 234), (2712, 234)),
((2200, 39, 235), (2019, 235)),
((2191, 23, 236), (2352, 236)),
((2177, 21, 237), (647, 237)),
((2172, 26, 238), (2252, 238)),
((2166, 20, 239), (3044, 239)),
((2160, 26, 240), (2907, 240)),
((2156, 25, 241), (1621, 241)),
((2147, 19, 242), (2407, 242)),
((2145, 21, 243), (2231, 243)),
((2143, 21, 244), (3905, 244)),
((2142, 20, 245), (2107, 245)),
((2139, 20, 246), (2127, 246)),
((2136, 26, 247), (2261, 247)),
((2133, 20, 248), (1985, 248)),
((2127, 22, 249), (1558, 249)),
((2126, 21, 250), (2760, 250)),
((2121, 21, 251), (2066, 251)),
((2114, 25, 252), (2262, 252)),
((2114, 19, 253), (1819, 253)),
((2107, 26, 254), (1666, 254)),
((2107, 22, 255), (2046, 255)),
((2106, 24, 256), (1429, 256)),
((2073, 19, 257), (798, 257)),
((2069, 21, 258), (4262, 258)),
((2066, 22, 259), (2121, 259)),
((2053, 21, 260), (2053, 260)),
((2046, 22, 261), (1797, 261)),
((2042, 24, 262), (1302, 262)),
((2038, 23, 263), (1435, 263)),
((2037, 23, 264), (1440, 264)),
((2034, 20, 265), (2739, 265)),
((2030, 26, 266), (1680, 266)),
((2027, 21, 267), (3568, 267)),
((2024, 20, 268), (3002, 268)),
((2023, 23, 269), (1416, 269)),
((2022, 23, 270), (1295, 270)),
((2020, 20, 271), (2643, 271)),
((2019, 22, 272), (3257, 272)),
((2013, 27, 273), (1327, 273)),
((2013, 21, 274), (2425, 274)),
((2007, 29, 275), (1631, 275)),
((1985, 22, 276), (2218, 276)),
((1984, 19, 277), (1904, 277)),
((1983, 21, 278), (1700, 278)),
((1981, 19, 279), (2744, 279)),
((1977, 21, 280), (1547, 280)),
((1974, 19, 281), (1491, 281)),
((1971, 25, 282), (2471, 282)),
((1958, 20, 283), (1468, 283)),
((1956, 19, 284), (1383, 284)),
((1955, 20, 285), (2884, 285)),
((1950, 25, 286), (1822, 286)),
((1939, 22, 287), (2572, 287)),
((1935, 24, 288), (1595, 288)),
((1928, 19, 289), (1441, 289)),
((1919, 26, 290), (842, 290)),
((1915, 25, 291), (2325, 291)),
((1914, 20, 292), (1983, 292)),
((1905, 19, 293), (1726, 293)),
((1904, 21, 294), (2307, 294)),
((1900, 20, 295), (2287, 295)),
((1891, 21, 296), (2177, 296)),
((1888, 20, 297), (3563, 297)),
((1877, 28, 298), (1182, 298)),
((1869, 23, 299), (1774, 299)),
((1861, 19, 300), (2980, 300)),
((1860, 19, 301), (2145, 301)),
((1851, 21, 302), (1891, 302)),
((1846, 23, 303), (2143, 303)),
((1845, 33, 304), (1105, 304)),
((1831, 26, 305), (3377, 305)),
((1822, 21, 306), (2027, 306)),
((1819, 23, 307), (1699, 307)),
((1819, 22, 308), (2223, 308)),
((1815, 20, 309), (2542, 309)),
((1811, 26, 310), (2069, 310)),
((1806, 27, 311), (2891, 311)),
((1797, 21, 312), (1977, 312)),
((1782, 25, 313), (2438, 313)),
((1774, 21, 314), (1085, 314)),
((1763, 22, 315), (1851, 315)),
((1748, 27, 316), (2013, 316)),
((1746, 31, 317), (1427, 317)),
((1742, 20, 318), (3163, 318)),
((1726, 21, 319), (2852, 319)),
((1713, 23, 320), (1320, 320)),
((1700, 21, 321), (1240, 321)),
((1699, 21, 322), (2126, 322)),
((1695, 24, 323), (2501, 323)),
((1695, 20, 324), (865, 324)),
((1687, 20, 325), (1955, 325)),
((1685, 24, 326), (1483, 326)),
((1681, 19, 327), (1100, 327)),
((1680, 21, 328), (1914, 328)),
((1679, 23, 329), (1036, 329)),
((1667, 20, 330), (1695, 330)),
((1666, 22, 331), (2472, 331)),
((1661, 20, 332), (2142, 332)),
((1661, 19, 333), (1396, 333)),
((1650, 26, 334), (1052, 334)),
((1647, 20, 335), (1687, 335)),
((1644, 19, 336), (834, 336)),
((1641, 26, 337), (962, 337)),
((1636, 25, 338), (1160, 338)),
((1631, 21, 339), (1072, 339)),
((1621, 22, 340), (1587, 340)),
((1615, 20, 341), (1425, 341)),
((1610, 19, 342), (1742, 342)),
((1595, 21, 343), (3515, 343)),
((1587, 20, 344), (1192, 344)),
((1585, 19, 345), (2828, 345)),
((1585, 19, 346), (2799, 346)),
((1578, 23, 347), (1463, 347)),
((1577, 23, 348), (2599, 348)),
((1575, 23, 349), (2034, 349)),
((1558, 22, 350), (2166, 350)),
((1549, 24, 351), (1647, 351)),
((1549, 20, 352), (1615, 352)),
((1547, 21, 353), (1549, 353)),
((1544, 25, 354), (1107, 354)),
((1535, 22, 355), (2279, 355)),
((1525, 26, 356), (5686, 356)),
((1522, 19, 357), (3106, 357)),
((1508, 23, 358), (2133, 358)),
((1506, 22, 359), (1212, 359)),
((1491, 21, 360), (2139, 360)),
((1490, 19, 361), (992, 361)),
((1483, 20, 362), (1446, 362)),
((1474, 23, 363), (3666, 363)),
((1471, 22, 364), (1667, 364)),
((1468, 21, 365), (2024, 365)),
((1465, 24, 366), (2235, 366)),
((1463, 20, 367), (2219, 367)),
((1461, 23, 368), (2611, 368)),
((1457, 25, 369), (2659, 369)),
((1455, 26, 370), (1661, 370)),
((1449, 24, 371), (2933, 371)),
((1446, 20, 372), (3389, 372)),
((1441, 21, 373), (1900, 373)),
((1440, 21, 374), (2439, 374)),
((1435, 21, 375), (2020, 375)),
((1429, 22, 376), (1055, 376)),
((1429, 22, 377), (3445, 377)),
((1427, 21, 378), (2560, 378)),
((1425, 20, 379), (3027, 379)),
((1416, 21, 380), (1958, 380)),
((1410, 20, 381), (3392, 381)),
((1406, 20, 382), (3160, 382)),
((1396, 20, 383), (3064, 383)),
((1394, 19, 384), (2350, 384)),
((1383, 21, 385), (2452, 385)),
((1354, 22, 386), (1255, 386)),
((1327, 21, 387), (1410, 387)),
((1322, 28, 388), (1888, 388)),
((1320, 21, 389), (1815, 389)),
((1302, 21, 390), (2954, 390)),
((1295, 21, 391), (1406, 391)),
((1277, 24, 392), (2226, 392)),
((1277, 24, 393), (3252, 393)),
((1269, 19, 394), (3667, 394)),
((1255, 20, 395), (1061, 395)),
((1248, 24, 396), (1104, 396)),
((1240, 21, 397), (1956, 397)),
((1212, 20, 398), (1984, 398)),
((1192, 20, 399), (1974, 399)),
((1184, 23, 400), (1269, 400)),
((1182, 21, 401), (1585, 401)),
((1168, 23, 402), (2460, 402)),
((1160, 20, 403), (1132, 403)),
((1139, 19, 404), (1116, 404)),
((1132, 19, 405), (566, 405)),
((1116, 19, 406), (1139, 406)),
((1107, 20, 407), (1394, 407)),
((1105, 21, 408), (2634, 408)),
((1104, 19, 409), (1661, 409)),
((1101, 24, 410), (2632, 410)),
((1100, 20, 411), (2880, 411)),
((1099, 23, 412), (2284, 412)),
((1094, 19, 413), (2114, 413)),
((1085, 21, 414), (1681, 414)),
((1072, 20, 415), (2073, 415)),
((1061, 19, 416), (1522, 416)),
((1055, 20, 417), (1644, 417)),
((1052, 20, 418), (817, 418)),
((1036, 20, 419), (1981, 419)),
((992, 20, 420), (1928, 420)),
((962, 20, 421), (695, 421)),
((948, 19, 422), (948, 422)),
((918, 22, 423), (3658, 423)),
((865, 20, 424), (1905, 424)),
((842, 21, 425), (1490, 425)),
((834, 20, 426), (1094, 426)),
((817, 19, 427), (1861, 427)),
((798, 21, 428), (1585, 428)),
((695, 19, 429), (1610, 429)),
((647, 22, 430), (2147, 430)),
((566, 19, 431), (1860, 431))]
In [158]:
#How many are non-unique due to HpaII/ScrFI overlap?
sum([(item.guidecount - len(set([str(guide.seq) for guide in item.guides]))) for item in exp50bylen])
Out[158]:
555
In [190]:
for item in exp50bylen[0:432]:
print(str(item.start)) +"F;"+ item.primers["leftprimer"]
for item in exp50bylen[0:432]:
print(str(item.start)) +"R;"+ item.primers["rightprimer"]
77581F;CTTCCAGCCGGTAAGTTCTGT
256392F;GCAATGAGACTCCCTCTTTCCA
213752F;ACGATTAGTTCCAGTCCGATACA
205910F;TGTATCTTCATGATGCCCTGTTAAA
57364F;ATGTCAGCCATAACCCAGGC
364209F;TAGAGGCCCTCAGGATAGCC
289188F;ACCCAGTGGACTATGACTAAGAGA
82592F;TTTTAGACCTGGTCCCTGCG
287246F;ACAGAATGACCAGGGATGTGG
226998F;GCCTCTGCACACATGGTAAAG
206079F;CCACACTAGGCTGAAGGC
242527F;CAGGAGGCTGCTTATCAGGG
216162F;AGCGGAGACATTAGCAGTTGT
343556F;GGAACATCACACTCACAGCC
113849F;CACTCTACCGGTCTGCAGTC
62894F;GTGTCTCCTGCACCTTGGAA
146388F;ACTCCACAGTCGGCTTCTTG
18759F;GCACCCACCGCTCCTTTTAT
167825F;ACTCGGCATTAGGAGTTAGGAAA
413023F;CCTGGGGGTTCGATTCTTCG
194704F;GGGAAAGTGTTCTCTTGGGGT
388659F;GCCGGCGCCACATAATTG
187606F;ATTCACCTAGATGGGGCACG
67282F;AGCGCTGGAATTGGCTACAA
205381F;CCCTTACCTAGTGTACCAAGGTC
92094F;CCTGCCTCTCAAGCACCAAT
279065F;GCTGTGGACCTAGCTGCC
129253F;TGCCAACAGTCTAGGGGAAA
141360F;AGCCTGGTTTGTCCCCATTT
340762F;GTTGAATAGGGCACTGGGACA
414279F;GGATTTATAAGCCATTCCTAATTGC
59675F;GCAGCCTAGTTTATCCCTGACA
85399F;ATCTGGGTTTTGCACACCCC
395769F;TTCTTCCAACGCTACCCCTG
297780F;TGGGCTAGTTTGCAATCCAGT
264614F;ACATGGACTTGGGCCTCATG
50159F;CCAGGGTGGGATGGCTCTTA
342572F;GCTCTGCTTACTATATCGGCACT
332312F;GAGGTGCAAAAGATCCCTCC
237607F;ACGAATAACCTCAGTCTCCCA
328902F;AACTTGCTCAAGCCCAGAGG
100915F;GGAAGTGACTGCCAGCTTTG
437410F;GGGTTGTAGTTTCCCTGCCT
258025F;TGCATCCCCCAAAGTACTCA
362623F;GGCTGCTAGTGTAAAAACGC
352403F;CTTCCAGGAGGCTCTACAGC
192325F;GTTATCTGCTAGTGTGGAATCTGA
220359F;TTACCTGGCATGTAAGCGGA
443917F;CCTCCATCTAGCCCTGCTCT
125772F;AAATTGCACAGGGTGTTGGC
276582F;GTGCCTTTACTCATAGTTTCAGCC
118996F;ATGAGCCCCTGAGAATCCTT
162023F;AGGCTTGAACCTGTAGTACAAT
337112F;CACTCTAAGCAGGCAACCGA
194967F;ACAGCCGGCGATACTAAAAGA
128317F;ATGGCATGGGAGGATGTGTC
136252F;TCACTTTGCAGACGACCCTC
428468F;CAGATAATCCCTAGCAGTACATACA
81975F;ACAGGGTCTGTTCATACAAGTT
205963F;TGTTCTCTAGCACTTGTAGCAA
336852F;GGTTGGTGCTTGGGGTTACT
302884F;CCACTCTAGCTAACCATCAGTGT
124848F;CTGCACTGAGGCTAATGGGA
306010F;CCCCGGCGCTCTTTCTTAT
33883F;TGGACACCTGGAAGTTATCAGG
101852F;ACAGGAGTCCCCTACACAGA
56941F;AGAGAGCTAGGCCTCTGCTT
45998F;GCAACTGCTTTACTCAGGGC
341254F;TCTGCCGATACCCCATGTTG
39420F;CTCCCTTCAGATGGGCGAC
97640F;AAACATTGCGGTGGGCCC
175684F;TTTCCTAGCTACTCTTACACGTGG
91629F;GGGGAAAGCAAGCTGGGAT
316151F;CTTATGGCCGGCTATACCCC
45251F;TGTGTATAATCAGCTTAAGCCCCA
166403F;TGTGGGTCAAGTGTTAGATGCA
242301F;GCTGGACTATGAATTATGGGGC
327230F;TTTTGAAGGTCAGTGCCTGC
68358F;CCAGCCCACCATGTAACGAT
45580F;CTCAGAGATGCCGCTGCC
329364F;TGTTGTTGACCCATGCTGGT
123929F;CTCCTGGGTATGCCACACC
136768F;GCCGCATTACATCCAGCAAG
336320F;GTTTGGTGCCTGTGTGATCG
325636F;TCCCTGGTAGCAACGCTTG
167923F;CCATGCGGGTTTATATCTGAAT
215563F;ACCCCAGCGGTACCTACTAG
97853F;TTTCTTTGTCCTGCAGGGTCT
146150F;TGCCGGTGTTACAGTGGATC
199895F;CCTTCTTCCAGGCACCATTTG
446363F;CAGGGGTTGCCCTAATGGAA
95120F;CACGGAGATGCACCTATGGG
221129F;GCCTGGGCTGGAAGTGAAT
383628F;TGCGACCCAGTTGTTTCGTA
194103F;AGCCCTCTAGGTTCTGAGGA
384596F;CACCCATCTCTCCCCGGTG
76760F;AGGCCCTAATCTCTGTGTCTTT
252580F;TGTGCAACCTATGGCCCAAT
63440F;TGTGAAGAAGCACCCAAGGC
115444F;TGTCCGAAATGCATGAGGTGT
301372F;ATGAAGTCACTCCTGCCCAG
91801F;AAGGAGTATCTGCAGGTTACAGG
356458F;GCTTCTCCCACCAGTACGTC
307586F;GTCTGACCCTGGGTTGACAT
203370F;CCCTTCTGCACTATGACTCTCC
215217F;TGCAGGGCCTGGAATGTA
54177F;GCTCAAAGCTATGGGGAATTACA
31816F;CTGGAGTTCCACCCACATGG
326830F;TGCTGTCTGAGGTATTCCTGAA
150878F;ACAACCAGGTAGCACTCACC
240390F;AGCAGAGGAACAGGAATGGT
118340F;ATTTCTGCTTGGGTCCTGCT
235722F;CAGTAGTCACACTAAGATATGGTCA
139943F;CTCTAGGGCCCTGTCCCTTA
46855F;AGAAAACTAGTTTCCTGGAACTCTT
77841F;CCAGGCCATGGTTACAGT
135209F;TGCTTTTCCTGTGATGGCCT
100852F;GGCGTTGGCTTGATTCCATC
51732F;GGGGTGGAATTAGGCGAGAG
98041F;CCCTAGCACCAAACGCTCTT
425611F;GCCCTGGCCTGAACTTTGA
364140F;TGAGGATCAGAGTAGGGGGT
220236F;AAGTCCCTGCCAGGTAGTAGA
215713F;GATAGCACTAGAGGGGCGC
98165F;GAATCCTCTTGTCCCTGGCC
92591F;GGAGACGTCACTGTAGCATCA
93240F;AAACCAGGCTGAATATCAATGACA
92746F;GCTGTGTGTCAGGATGGTGA
101043F;TCCACTAGACACCAGCAAAGAG
19892F;ATCTGCATCCCTCCCACTGT
168026F;TCATAGTGATCCTTGGATGGCA
98138F;CGGTTGAGGTTCTGGCTGAT
142729F;AAGTCATATTGAAGGCACTGAAAC
249338F;CACAATTGCCACTGGGTTGG
165778F;GCGATTCACTGGGAGGTAACA
439089F;TGCCCCTGGATGGATTTTCAG
2456F;GAACACCAAACGCGTCATCC
96913F;ACAGTAACCTAGTGTATTCACCAAT
84921F;CCAGAGAGCCCTCCACAAAG
41196F;CAGCCAGCACACTCTATGCA
224450F;CACTGTGGCCCTCTTGTGAT
22404F;AAGCAGACCGGGTCACCC
64679F;CCCCTAGGTCTGCCCATCTC
57492F;TGATTGGCTGACTGAGTGCC
383860F;ACTCGGATAAGACTTGGGCAG
232823F;TCTCCCCTGGAATTTAGCACTT
111302F;CAGGCAAAAGCCCCCTTTTC
78598F;CATCCTGGCTCCCCATTTCA
345294F;CCCAGGAGTTTTCAGGGCTT
8070F;CCGTCCCACACATGGCTT
84880F;CTAGGGCTGCCAGTTAGAGC
191636F;CCCAAACCTGGTACCTTCCC
443682F;ATTTACTGGCCATGGCTGCT
179516F;TAATTGCCACAAGGGGCAGT
348089F;GCAGCCCCTAGTCTTATTCAAGT
71358F;TGCCCCCCATTTCATGATCC
62516F;AGCCAAAACGGTCACTGACA
257510F;GGCAGCAGTCAGTGTCTCTT
97186F;GGGCACTGAGTGAAACTCCA
18621F;ACACCGGGGGCTTAAATCTG
129494F;TTGCCATGGACCTTCTGCAT
305696F;CTGGTATAAGCCCTGCAGCA
336537F;ACACCTAATGCGGCACCTAA
226151F;GCAACTTGGCTTGGCACATAA
190692F;AAAGGCAGAGCTCCAGGAAT
38410F;TGGGCTTGTTCCATCCAGTT
192798F;AGAGTAAGTGGGGCCGGG
364860F;CCTGGGGTATCCTAGTTTCTCTT
282099F;TACTGGCCGGATAGCAAAGC
98734F;CACCTAGGTTGTGGGGACAG
348903F;TGGGATTTATTAGTGCTGTCCCA
50869F;CCCTTGTGCCGCTCAGTAAA
84225F;CTAGGCACAGGCTCCACAG
50792F;ATGGCTTCCGTTCCTCAGTG
304300F;AGCAGCTGGAGAATGGTACT
101098F;GGGGGAAGCGTAGGGTTAGT
83094F;CCCAAAGGCTAGATGCAGGT
231453F;TGGGATTACCGGCTTGTGTAGC
323488F;GTCCCTGGAATCACACAGGA
321443F;GGAGCCCTTTAGCATGAGCA
402522F;CTCTGCTCCGGGTCCTTC
102110F;TTGATGTAAATACCATCACATCCA
125450F;TCTGCAACAGACACTGGCAT
95243F;CGCTACCTGGATTCTTGCCA
203042F;GGACGGTACCTTCCTGCAAA
123628F;GGCAGTATCCTAATGGCTGTG
3328F;AGGCCTTGTTTGAAAAGCCT
306645F;AACTAGGCATGACTGGGGTG
46827F;AAGACTATCTAGCACACCACA
220644F;TGCGAGTGCCCCAAATCTAA
138452F;GGCCTTGAAACCAAGCAGTG
392291F;TTCAGGAGACCGGACCAACT
59039F;TTACTAGTCCCCTGTTCCACT
150526F;GCCCATAGCCTGTCACATCA
408182F;ACTTCTCTAAGGAGACACGCAAA
300912F;AAGGGGCAATGCTTGGAAGA
259444F;AACTTTGGCACATACGCGTT
420544F;GAAATGTGTGCTGGTGGTCC
8544F;AAGTCCTGGACAAATGCCCA
3035F;GGATGTCTAGGAGCGCGTTT
135361F;CATTCCAGGCCCCTGTCTG
364482F;ACAGAATGGCCCACACACAT
171570F;GTTGGCTCCCTCTTCTGTCC
391780F;GGGGTCTGAGGGGACATAGT
406750F;TGTGGTTATAGTGGACAATCAGCT
349939F;CCACTTCCGGGTGCTGCC
194324F;ACACTAGGCAACACAGAAGGTT
32755F;AACCGGATGCTTCTCTACAC
429634F;CCCTGTAGTACGAGGGGTTA
153380F;TGTCGGGATTCATTACTCACACT
88300F;ATCTAGCCCTGTGAGCCTCT
175257F;TGGCAGGCAATTCATGGTACT
131055F;CCCAGGCAAGCAGAGGTT
43859F;CGAAGGCCAAATGACCTGC
209975F;AGAGCATTAGACTTCTGCAGAGG
372592F;CAGGGGTTCCTTGGAAGACC
130644F;ACACACTGACCGATCCCATG
27043F;TGGAGAGTTAGCTGTGGACCT
352639F;CCCTGGAGAGATGTGAGCAC
301620F;CCCCAACACACAGATGACCA
145527F;AACCAACTCTAGTATTTACCAGCCA
47171F;TCACACCTGGAAAGCATTGA
299809F;ACCCTAGTCGCAAAGAGCTC
349007F;ACAATCTAGTGCCACCCTTT
387995F;CTGTAGGAGTCAAGCCGGAG
224317F;CCGCACAAAGGCCAATAACA
221097F;GCAACACTGTTCTTAACGGCTT
339194F;CCTGCTGTTCTTCTGAGGCT
341157F;GCCATGAAGACAATGGAGGGA
377401F;CCCTGTGCCTAGTGATGG
27560F;AAACCCCAGAAGAGCAGTGC
125946F;GGCACTGTACCAGATGGGGA
340804F;CCATCACAGCACTGGGTGGC
133328F;GCTCCACAGCAAAGTCCTTT
62766F;TGCCGCCATTTTCCCCTTAT
44672F;AGCTTAAGTGCCCCTGTCAC
399628F;TGCTGACAGAAGGGAGGAGA
190636F;ATGCTAGAGAGACAATCCTCTT
148959F;CAACTAGACTTCCTCATTGTTTCT
359886F;ACAGATTCCTTCAGCTGCCC
439582F;TAATGACCAGCCAAGCCCTG
220416F;CCCCGGCGGACTGGACTT
97560F;CCCCGGGCAAATCTGTCC
26170F;TGGAACCCCAGTCAAGCTTG
146842F;TCTCCTAGAGAAGAAGCCAGT
384709F;CCTGGGACAAGCCGTGTG
218825F;AAGGACTAGGGGTTAGGATTCCT
439419F;GCAAGATGCCAGAGAGGTGA
191868F;CCTGTGTCTATGGGGCCAAG
385050F;CAGAGACCCGGCTCTCAG
261302F;GGAGTCTGATGGCCACACAT
187272F;TTCCAAGAAACAGCACAGCC
142838F;GTCGGGCACAACAGTTTTCC
267116F;TCCCCACACCTTAGGTCACT
445617F;CAGTGCGATGGGAGTGGAC
338893F;CATGATACCAGGTAATCTCTTGATG
260786F;CAGACGAAGGCAGCTTGTCT
63996F;TTTCTACGTCACGTCCCTGG
19126F;AATCAGAGCACCATGTTCGGT
331997F;GGCTTTCAGTTCAGTGACCCT
114557F;AACCCTGGAGTCAACTATTGA
287853F;AATTCTCTGTGGGGGTGCTTT
343692F;ACAGATGTGCAGGTGTGAGG
352579F;TTGTAGTCCAGGCAGTTGGG
124239F;CGGGGCTACTTCGGTGTTAA
336576F;CAAATGCGCTCTCCACCTTG
243055F;GCCAGACAGCCAGTAGTAAGA
216012F;GCCGGGAAAAAAGGTCAGGA
366275F;CCGTGACCTGGACCCATATTT
261436F;GCGGTCTGTGTCTTGGGTAA
76078F;GGTGTGCGTGGTGTCTCTTT
145700F;GTTGGACAAAGTAGGGCCAGA
360894F;GTGCAAACCCACTGCTTCAG
40387F;CCCTTGGGCACTTGAACAGT
302032F;GCTACCACTGGACCCCTCTA
301991F;GGCAACCCTATTCAATGAACCC
142467F;TGGCCTCCCGATAGTAAAGT
438895F;GTAACCAGCTAGTTAATCAGTAGTT
42071F;ACTATGTCCGGAACCCCATG
192285F;ATATACCCCAGGAGCAGCTT
80170F;AATCTTCCAGCGGCAGTGTT
4629F;CCAGGCTGGGGGGTATCC
30304F;CCGGAGCTTGATGAACTGGT
253161F;CCATCATGCCTGCGAAACAA
18856F;GCATCCCTGGCCCAGAGATT
49027F;TGGCCATATTGAAACAGCCCT
109529F;TGTACAATCCTCTCGCCTGTG
213562F;CGAGTGGGCCCTCATTTTCT
95184F;TACTCAGCACCCTGGAGAGG
203092F;TTGAGCCTGTAACAATGTCCT
100675F;CCTGAGACTGGTCCAATGCT
172226F;GATGGACTAGCCCTGTGAGC
99310F;GGAATCTGATGTGTGGGGAGA
343260F;TAACCATGCACAGCAGCCAG
321719F;CTAGACTGGCCACCTGGAAC
123677F;CTGTCAGTGGTTCCTGGGAA
64561F;CTTTTTCTAGTCCCCACCTCCA
197877F;TTCCCTTGAGTCTCGAAATCA
128879F;AAATGCAAGGGACAAAGGCA
244070F;ATTGATGCACCTCCACCCAG
68396F;CTGTCACCATCCAGCTGTTA
112262F;GCCTGACCTACCACTTGGAG
151702F;TGTTTGGCAAAGCGGAAAGG
350346F;TTTGGCTGGTTTTGAGCAGC
355528F;ACCCAGCACAGACATGATTAGAG
35682F;TAAGTGCCAGTGTGGGTGTC
239534F;CTAGAAGTCAGGCGGCATTT
29804F;AATGTTTTCTCCAGCGCTGC
57888F;TCTCCCAGCAATACTTTGTGCT
201603F;CAGGTATCCTCTATCCTCTTTCAA
9552F;ATGCCTCAGTGCTTCACGGC
252477F;TTGGATCCAGGTTAGCATATCTGG
438966F;AGTGCCTTTGTCGTCACTCC
300880F;CCGGTTCCTGTGTTTCCCTA
295036F;CTATGACCTTGTGGCCCAGG
118582F;GCCAAGATGGACAGCGAGT
413540F;AGCTGAAGGTCTCTGTCCCT
396596F;TGGGGGCTCTACATCCTTCA
175082F;CCAGTCCAATGGCAGAAGTA
93422F;AATCTTACCAACCGCCTCCG
341892F;CGCTGCGCCACTGATATATTC
338450F;TGAGGGACCAGCCACCAATA
240883F;TTTGGCATTATTGTCCTATGGTCA
68301F;CCCAGGAATGGTGCAATAGCTTA
12762F;CCGGCACAACTGATGGGAAC
34291F;TGGTAACTGGCGTTCTCACC
141260F;CACCGGCTAGTACCTGCAG
355585F;GCAGGGGGCATCTTCATATCA
419014F;CTCATCCGGCACAGACAGAG
68079F;GCTACACAGGAACCGTTGGA
353453F;TCCAAGCTAGGCAGAGTGGA
172755F;TGCTCCTAGAGTCTGCGATACT
117597F;CGGCCCTGCACTTATCAGTT
20828F;GGGCTCTGTTGATGTGGGAT
221200F;CTCATTGGCCCCCGTACG
416138F;CTGGCAACACCTCATGGACA
92462F;GGTTCCCATGATATCCGGGA
384476F;AGCGTAAAGTGCGAAGAGGA
40119F;TCCTCCCTCTAGCTGTCTCC
124212F;GCCGGGAACAATACTACACT
171913F;AGTACTAGCGTGCCCACAAA
17675F;GCCGGATCTACTAACGAGTCC
77946F;ACTAGGGCCCAAGTCAAGTT
347198F;GTAAGGGCCTGGAGTTGCAT
51639F;ATCTAGGGTGGCCTGTGTGA
260574F;TCAGGGCACTCTAGGAGGTT
70643F;CCAGGCATGATCCTAGCTGT
101881F;GCAAGGGAGATATAAGTGAAGAGCT
12877F;GGAGACTTTAGGAGTAAAAGAGTCT
314079F;GTTGCTAGGTAGAGTGACGTCA
142787F;TCCCCAGGAATGATGACAATCA
63124F;TGCAAGGCAAGAGGGAACAA
35760F;GGGGAATGTGCCACCATCTT
44615F;GGGATGGCAAGATGGAACCA
32890F;GCTTCAGCCCGGAATTTCAG
407099F;ACCTCCAGCCGGTCGTGG
222609F;ACTAGCAGCGCCTGTTCC
76855F;CCACAAGGCAGCTAAAGGTG
1202F;CCACTGGCCCCTTCTTTTCT
217673F;ATCCTGGCCCTGCTCTGA
55685F;CCAGGCGGTTGAGGTCAG
356056F;GGATTTCGCTGCTGGCAAAT
50837F;GTTCCAGGTAGGTACTGTGCC
29602F;TGCCAGTAACGTACAGCCTG
34185F;AGCAAGAACGCGATTGGTTT
87020F;CCTCTCGCATCCAGGGAAAG
323970F;GCAGCCGCTGTGATTCTAGT
172444F;TTGGATTCCTATGGCCAGTAGC
33957F;CCGGCACCCCCACCCAAG
245975F;AGTCTTCCTTTGCAAGCCTGT
56086F;AAGCAACATGGCGCAAAGTT
168085F;GGGGGGCTAGACTGAAAGTG
339387F;TGCCACTTCTTGCCATGACA
364672F;GGGGACACTCATTCCCCTTT
13658F;CATGCTGCGGTTAGAGAGGG
451505F;TGCCTGGTTGGTCAGAAAGG
43416F;AGCTGGGATTAGGGTTGCAC
362044F;TAACAACTGACCGGTTCCCC
303704F;CCCTGTGGATGGAGGTTGTT
99689F;CGAGCCTAGATCCCTACGGT
148683F;CCTGGAGGGGCGCATTGG
138480F;ACCTATTCCTTGTGCCGGTG
219992F;GGTTTAGGCCCAGCTATGCA
409232F;GGCAAATGGGTCATTGGGTG
129078F;GCCATTCCCTTTATGCCAGC
125717F;AGCTAGCTTGACCTCATGGA
100084F;CATAGACTAGGCAGCGGCAA
78301F;CCATTTCAAAAGCTAGGGGTGT
70988F;TCACTTTTGCCTGTGGGAGAA
205997F;TGATAAGCCACCTAGACATGGAA
331742F;ATGGTGGGATTCCCTAGGCT
285096F;TGGCCTGGTGGTCAAGTAATT
142149F;GCCTAGGGAATGTGGGAGTG
44930F;CGGTGGAACTGTGACAACCA
33592F;GCCCGACTTCTTCTGGAACT
82745F;AGTGTCTCCTGGTAATGCAC
389468F;GTATGTCTCTGGTTGCACCCT
254241F;GCCCTCATGCGCTTCATTAC
208366F;CCGTTACAGCAAGCCATGTC
16227F;TCTCAGAATGAAATGCCGCCA
13181F;CGAAGTCTGATTTGCTGTGA
288020F;AAGGCGGGCTTAAAACAGGA
1428F;GGGCTACAGTCAGGGACCA
27619F;GCCCCAAAGCTAACACTCCA
50198F;ACCTGGCGCGTGTAATAGAG
324128F;TTCATGCAACCCCAGCACTA
409502F;AATGGGGAGGGTCATGATGC
41673F;CCCCTCTTAGGTCCCCTCAT
187653F;GGGCCTTTACTGCTCGTTGT
359057F;GGGAGGGCTACATTAACAGCT
411033F;GCCCAGTCTTTCACAAGGTC
60328F;CTGCCACTGTCATGTCCCTT
15072F;CCCGGGGCAGGAATGGCA
51596F;CACTGTGTGACTGGGGGAAT
145840F;TTCGTTGCCACCCCTTTCC
45646F;GCCTCAGTCTGTAGGTCTGG
347983F;GGTCACTAGAATAGGGGGGCT
219156F;GTCCGGACACTGCCACAG
92486F;ACTGTGACCCAAGAACAGCA
444797F;TCAGACAAGCAAAGTGCCGA
259223F;TGGTCCCAGGTACCTTTCCT
183897F;ATTAGGCCGGTTACTTGGGT
323150F;CCAGGCCAAGTCAAAGACCT
332149F;CAGTGCCGGTGAGTCTGAC
388276F;TGACTAGAAGCTTTCGGCCC
367783F;GCTGCTTACGTTTAGGGCCT
41887F;CTCCCTCTGCCTTTGTCCC
120611F;AGGGTAGGAGACATATCCCA
301286F;AACAGATGCTGCCTGGACTG
315103F;TGGTGTCTTTGGACCCACTG
10358F;GGGGCAATGAGCTGCATCAC
421358F;GCAACCAGTTGACATGTCCC
77581R;GGTGGACCCTGCAGCTATG
256392R;CCCCATTTCCCCCTTGTACC
213752R;TCCCCTGTAGCTAGCTCGTT
205910R;TAAGGGAGGGCACACAGTAC
57364R;TGCACTGCCCGTTGTTATCT
364209R;ACAGTTCTAGTTGGTTGGCCA
289188R;TCCAATAGCTCAGCTGGCAG
82592R;CAAATGCAGTGCCGGGTATG
287246R;ACACCAGGGTTTACAGGATGTC
226998R;TGCAGCGGTTCTTTTGAAACA
206079R;GAACAGCTAGGGAATGGGGT
242527R;GGGTGAGATTTGTGATGCTTGG
216162R;TCAGAAACACGTGCATTCTTGTT
343556R;GGAAAGTTGCCTGGCGGA
113849R;AGCAGCTTGACCATGGAGTT
62894R;ATGGGATCTGATCACCCCCA
146388R;AACCATCTTCAGCACGCTCA
18759R;GGCGTCTCTCTCCCCAGATA
167825R;GTGGGTAGCCTGGCTAATGT
413023R;CAAGCAGCAGAGAGACGGAG
194704R;GCAATGCTTGTGCCCTTTCT
388659R;GGGGGAGGCAGTTGCTAAAT
187606R;TTAAACTAAACTTGTACCCTTCGT
67282R;GCCTCCGGCCCACCCTAA
205381R;AGTGAAGAAACTAGCCACCAGT
92094R;GCACATGTGTTACAGCCTGC
279065R;AGTCTGACTGGACCTTGGGT
129253R;AGCAAGATGGCACTGATGTTT
141360R;GTGGACGCCAAGCAACTTTT
340762R;TCATTTGCAAAGCCACGGTG
414279R;TGCTCAGGTCTCTCTAGTGA
59675R;GATGAGGCCCTCTCCCCTAT
85399R;CCCCGGCCTACTCACGAG
395769R;CCAAAAGCCACATGGGGATG
297780R;GTGTTTGGGGGTTGAAGCAC
264614R;TGCAACTCTGGGTTTGGTGT
50159R;CCAACCTGGCCGCCATAG
342572R;TGTGAAATTGGGATCAGTCTTGC
332312R;GGACCTGGGAGATCTATTTGT
237607R;GAAGCATAAGCGTCAGGTGT
328902R;CACAAAGGCCCGGTCCAG
100915R;GCAAAAGGTAGAAACTACACTCACT
437410R;CACAGGCACTTGCTGTTTGT
258025R;GTGTCTTGCTAGCAACGCAT
362623R;TTGGGGGGTATGCCATTACG
352403R;CACTCACCCAGGGTAAGGAAC
192325R;AGTAGCCAACACAGCTCACT
220359R;TGTGCAGTTCTAGTCTGTGTGA
443917R;TGGCAATAGGAGTGGCCAAG
125772R;ACAAGAGGGACAGCGACAAG
276582R;CGCAGGATGAAATGCCTGTG
118996R;TTTCAGCCAGATGTAGAGTCTTTAA
162023R;CAGGAGAATCATGGCAACGC
337112R;CATGGGCAAAGCTGGAAACC
194967R;GCCGCCAACTGGTGTTTTAA
128317R;CACACAGGCCCGTACAGAC
136252R;AACAGCTTAGATGAGCCGGG
428468R;TTCACCGGAAAACAGCTTGT
81975R;TCCGGTTCTTGTTGTGTTCT
205963R;GCAAATAGCCTTTGAACCCC
336852R;TTGGGTTTCCACCTCACCAC
302884R;GGCACCCAGGTCTTAAAGGA
124848R;CGTTTACCTGCCCCATTTGG
306010R;TTTAACCCCCTAGAGGTTTCTTT
33883R;AGTCTGGTGTTGTTGCTTAGGT
101852R;TGGAGCCACTTATTCCACGT
56941R;CCTCAATTCACGCAGGAGGT
45998R;CCGCCTCTGGAATGATGTCA
341254R;GCCATAACTGTGATTACAACAAGCT
39420R;GTCCCCTTTAGATCCCCCCT
97640R;CCCCCCCAGGCATTCCTAT
175684R;GTGGAAGGTGTGGGTCCATT
91629R;TGGGCAGACACCAGAATCAC
316151R;ACTGCTCCTAGCTGCCCT
45251R;TGTGTACTGATGCCGGAGTG
166403R;CCGCAAAACACCTGCAATGT
242301R;GGAGGGTATGGGGGTATTGG
327230R;AGTGGTTTAGGCGTAAATAGGGA
68358R;GCTTCAGTTATACCACTAGACAGCT
45580R;GCCCCTACCAGACACTTATTCC
329364R;GCTGGGCCTTGCAGGTAATA
123929R;GGAGCACCATAGCTGAGGTAC
136768R;GGGGGAGGTAGTTGAGGAGT
336320R;TTCCTGCTGGTGGCTTTGAT
325636R;ACCCCAACCATGGCAAGAAA
167923R;GCCAAGAGCTTTGAACTAGG
215563R;GTGCCAGAGTTAGCCTGGTT
97853R;GCCACTCATTTCCCTGGTCT
146150R;GGCTAGATGCTGACCACAATGA
199895R;AGGGAACAAAGGCTCCCATG
446363R;GACTTTGCCAACCTTGTGGG
95120R;GGCACCAGGTATAGCATTACAGT
221129R;TATATAGCCCACTGCCCCCA
383628R;CCCTATGACCCTGGTTTATTTCTCT
194103R;GCCTGTCCAGTAATGCATAGC
384596R;CGCTGGCCTGGCTTTTGG
76760R;ACCAGGAAGCTGCTTATCGT
252580R;ATTACAGCAGGAGGGACCCT
63440R;CCACTGCTTTCTGGCGCTTA
115444R;AATGCATCAGCCAGCTGGAA
301372R;CAATAAGCAGCTAGTTCTCATCAG
91801R;CGTGGGTCCATCCATAACGT
356458R;GGGCAAGGGAGCTGAGAAAT
307586R;AAATGGACATAGCATGACTATCCTG
203370R;TTACTGTGTTGTGCTGCTTGC
215217R;GTAGCGGCCTCTTTACCTGT
54177R;TTCTGTAGGACTAGTGTAGATGCA
31816R;GAGCACCTTCGCCGGACC
326830R;AGTCGAAATTAGGCTGCCCT
150878R;CTGCAGAGACCCACATGTGA
240390R;TGCCCTAACTTCCACGTCAA
118340R;GAGAGCCCCCATGTTGCTTA
235722R;CCAAAGCTGAGAATCCCACC
139943R;ACAGACGACAGCCAGATGTG
46855R;TACTGCTCAGCCTACCACCT
77841R;CTTCACCGGCAGGCAAAC
135209R;ACTCAGTAGAAGCTGCTGCAG
100852R;AGGCTGATGGGGTACACTCT
51732R;CCTCACCTCAAACCACTGCT
98041R;GTAGGACCTTCTAGCTGTCCT
425611R;ATGGCAAGCAGGGAATGTGA
364140R;TCTTTTTTTGGTTAGCCAGGAAGT
220236R;GGAGCCCACCCAGGTAAATT
215713R;TGTGCTGCTTTCTACTGACTGT
98165R;GGTTCTCAAATAACCGACCCAGA
92591R;TCAGCCCCATAGATAGGCCA
93240R;GCCTTCCCCTGGCTGTATTA
92746R;CTCCTCACCCTCCTTGCTTG
101043R;GCACACTGCCTACCACTCTT
19892R;GAATTCCCCGCTAGGTGGC
168026R;CAGATATAACACCTGGGAGCCA
98138R;TGCCTTGGTGCATTCTAGCA
142729R;TGCCACTCATCTACAAAGCC
249338R;GGGAGAGAAGCCAGAAACCC
165778R;GTACATCAGGGAGCCCATCG
439089R;CACCCCGGGCTCCTTACT
2456R;AAAGCCGGCGATGTCCAATA
96913R;CCGCCAGGCCTCAGATGG
84921R;CCTGGTCAAGAGTGGGGTTAC
41196R;TCCAGCCTCATCACAGCAAC
224450R;CCCAGCTCAGTTTCCATGGT
22404R;ATGGTCAGACCGGCCTATCG
64679R;CGAGCCCCGGCACTGAGC
57492R;GCTACGACCTGCTTCCCATT
383860R;CACAGGGGGCCAAAGAGTAT
232823R;GCTGCCCGGCATACCAAG
111302R;TACTCGGTGTGCTCTAGGCT
78598R;TATAGCGCTTCATCCTGGGC
345294R;AGACCTGGTATAGAGTTTGCAACT
8070R;AGGTGTGAGATGCCCAATGG
84880R;TGATGGGATCCTGGCCTT
191636R;TCTCTCCAGGCTAGTCTGCA
443682R;GTATGCCGGCAGTAGAGGG
179516R;CAACTAGCTGACCCCCTTTGT
348089R;ACATGCAGGGCAGACACTAC
71358R;GCCTCATGTGGAGCAACAGA
62516R;CAGGGCTCAGAAATGCTCCA
257510R;CTGATGGTCCTGGGTGCATT
97186R;GACCAGTAGGGGGAACTCCT
18621R;GTCTGGACACACGGCACTAG
129494R;GACACGGCGGCTTGTTATTC
305696R;TGAATTAAGGGGCCCCAAGC
336537R;GGGTTACCACCTGTTCCATCT
226151R;CCGCTAGTCTGATATGGAGTCAC
190692R;AGCTACTACTAGAGCAATAGGAAGT
38410R;GTTGTTCCTCCAGGTGCTTT
192798R;TCGGATCTAGGTGCAGTGGT
364860R;GGTTAGGAAGCCGGAGTCAT
282099R;TGGATACCTGCCTTGGGACT
98734R;TCCTAGAGAATACCCATGCAAAA
348903R;AGATCAAGCACAGGCACCAA
50869R;CCCTCACTTTATCAGCGGGG
84225R;ATCCGGGAGCTGAGGGTAAT
50792R;ACGAGAAGCCTTAGGGATGC
304300R;ACACAAATACCAGGACAAGAGA
101098R;ACCCCCTGGCACCAACAT
83094R;GGGGATAACGTTGTGGTCTGT
231453R;CACTGCATGATTCAGAGCTGC
323488R;GCCGGACAGTATAGCAAGTTTT
321443R;ACCACAGGAACTCACCACAG
402522R;AGCTATTTGGAGCCAGAGCA
102110R;GGGAGGCTAGTAGTGGCTGA
125450R;AAGCTTTCAGGATTGGCGGA
95243R;GTCTGCACTCTAGCCATGGG
203042R;TGCACACAAAACCGCAATGA
123628R;CAGATGGGAGGAAGGTGTCA
3328R;CTGCTCAACATTAGATGACAGGA
306645R;CCATGCATGGGAAGGGGTTA
46827R;AGGAGTAAACTGCCCAAAGA
220644R;CCGACTCAATGGTTCCAGGT
138452R;GGTTTTTGAGGGTGCAGCAG
392291R;TTCTCTTTGCCGGTCTCGTC
59039R;AAACCTGAGAAGCAGCCACT
150526R;AATAAAACCGTGACCCCCCC
408182R;TTGCACACACTAGCAGGAGG
300912R;ACCTTATGTCTGCCCTGCAC
259444R;TCCAAGAGTAGTCATTGTACTCCA
420544R;CCGTCTGCCTCATGTCTTCA
8544R;CTCCTGGCCTCCTCATGTAAG
3035R;TGGGTTGTATCTAGGTGTACCC
135361R;TGACCAACCTAGCTGGCAAA
364482R;CGTGCCCGACTTTACCCATA
171570R;GGAAAAGCTTCTGTTGGCCG
391780R;TAACGTGCCTGCCAAAAAGC
406750R;GGGTTGGCATCCTGAAGCTA
349939R;AAAACTAGTCAGGCCCCACC
194324R;CTCAAGATCGGCCCACCC
32755R;GGAGTCCTGGGAGATGGAG
429634R;GTATCCTGCTACTAGTATACTGCT
153380R;TCAGCTGCAGGATTCCATGT
88300R;ACCCATTCCTCCATCTAGTCCA
175257R;TTATTGGCTAAGGTGGGGCC
131055R;TCAAATACCAGTCTAGTGCCACA
43859R;TAAGCTAAGCACTGGGCCTG
209975R;GGTTGTCCTGGTGCTTCTGA
372592R;ACTGCATTCATGGGAGGGTG
130644R;AGCATTCGCCATAGACAGCA
27043R;TCTCCCACCCAACCTTCTCT
352639R;GCTTGTGCACCCCCAAATAC
301620R;GCCTTCTCAATGGGATTTGCC
145527R;CAGTAACTCCCCTCGGCTTC
47171R;TTTGCAGATATAAGTTGGATTCGG
299809R;AGTTCCATGCCCTAAAGCCA
349007R;GCATACCCTAGCAGAAGGTAC
387995R;GAGAATATAGTGGCCCTAGTAAAGG
224317R;GGTCTGTGCTAGTCTTCTGCA
221097R;CTAGAGCCTGGGGAGGGTAG
339194R;TGGCAAACATCAGACCCACA
341157R;CCCCACAATCCTTGCTCTGT
377401R;AATCATCTTGAGGAGGCCCC
27560R;GCTACCTCTGCCCCAATCTG
125946R;GTCGCCGGGACTCGCTGA
340804R;CAGACGCCCCCCGGTCTC
133328R;GTGGCTCCACTAGAAATTCACTT
62766R;GGTTTCCGGCGGGATTCA
44672R;TCTCCTGAGGGGGCAATACA
399628R;TCTCTATCCCTGGGAGTAGAGC
190636R;ATTGCTAGACCCTGGCCTAC
148959R;AACGCTCCTGCTCAACAATG
359886R;CCACATTGTCCTTCCTCCCC
439582R;ACGGTGCTAGCTCTCTCCAT
220416R;GTTTTAACCCCAGGCTGCTAAA
97560R;TGGGGTCCCACTATTCCTCA
26170R;GGGACCCTGGTAATAACACCTG
146842R;ATCTAGCCCTCTGTTGTTTGT
384709R;GTCACATGACCAGGGCTGAC
218825R;TCTGAAACTGCAACGGTCCA
439419R;ACAAGAAGCTTGGCCCATCA
191868R;AAAATATGACCGGGGGCCC
385050R;ACTTTTTGTCCTGGTATAGAACAGG
261302R;GCTCCCACTCCAGGATAAAGC
187272R;CATGTGACAGCTAGGGGATG
142838R;CTCCCTCTGGTCCTGTGAGA
267116R;TTGGACGCCCAGCATAAAGT
445617R;AGGATAATAACCCCCTCGCTCT
338893R;TCAAGAACGAGCACTTATAGAGT
260786R;CGTGCAGAGATTCTTAGAGCGA
63996R;ACCCACATTATCACTAAAAGCC
19126R;GTTATTGGGTGATGGGGGCA
331997R;GGAGTGGGGTTCCTGGGA
114557R;GGCCTTTTCCTAGTACTCCC
287853R;TGCTTCTTACTTGGGCTGGG
343692R;TTCCACCTGCGTCATGTTCT
352579R;TCAGTCTGGGCCTATCTGGT
124239R;TTCACTTTGGCCACCTACCC
336576R;TTGGTGCTACCCGTGTTACC
243055R;CCACTGGTACTGATGGCCAT
216012R;GCTAGGCAGGCATCAGCATA
366275R;CAGCACTGTGGTAGCTTCCA
261436R;GGAGGATAAGCATGGGCCAA
76078R;CACCCATCTGTGCCCAAGG
145700R;AGCAGGCAGTAATCCTCCAAC
360894R;AATCCTGGCTGCTTCTAGGC
40387R;GCCATCTTTGAGCAGCATGC
302032R;GCAAGAGCCAAGCTTAGCAC
301991R;GACTTCCTGGTAATGCCCCC
142467R;TTCTAGGAAAAGAACTCGTGATTTT
438895R;AACTTACCCTGGTGCAGAAC
42071R;TCTCTGTCTTTAGGCTGTGACA
192285R;GGAGCTGCGAGGGTAATAGA
80170R;CGGGAGGTTCTATGCGGAAG
4629R;CAGGGTTTCGGCCTAAGCTC
30304R;GCTGTGTTACTCTACTCTGACTAGG
253161R;TCCCCACTAGGCCAAAGAAC
18856R;ATACCCCCCTGCTAGCCCGG
49027R;ACCAAAGGCTAACCGTGGAG
109529R;ATTAGTCCGGGGGCCAGAT
213562R;GGGGTTGCAGTGGACCTTAA
95184R;AGAGCAGCTCTACCTACACCA
203092R;TCCTGACCGGCATTTCTAAC
100675R;TGCTGGCTGTGTTAGACTGT
172226R;CTGAGCCGGGAGATATCTGC
99310R;TGCTTGGCACTCATGTACCT
343260R;GGGGGCATCTCAGGGCAT
321719R;CTGTAGTGCAAAAGTATCAGCGT
123677R;GCTTAGCTAGCTGGAAGGTT
64561R;TAAAGGGGTACTGTCGTGCC
197877R;TTATTGCACGTAACGTTCCC
128879R;GCCTTTGTGATGGCATAGCA
244070R;CACTTCTATGACAATGGCCTGC
68396R;TGGGCTCAGTTCCTGTATAGA
112262R;TCCAATGAGCACCTAAACCCA
151702R;TCTGCCGCGTCTATTGACTC
350346R;GTTGCACGTTTCTGTACACACA
355528R;TCAGCCGCCGTGGAAATATT
35682R;CCATACCTCGTGGTTTCCCT
239534R;ACATATTATCTTGCGATCATTGTGG
29804R;TCTGGTTTGTGTGTCACGGA
57888R;CAGCCATAATAGGAGCACGGT
201603R;GGGTTGTAATGGCGCATTGT
9552R;GGGGCCCGGCACAGTCTT
252477R;GTGTCCTGGGATTGGAAGGG
438966R;CCACATGATCCCGGCACTC
300880R;AGATCAGAACAAGATGAGACCATT
295036R;GAATTGCCCAGGAATAGAATTCTGT
118582R;CCTTGATTCCGGGTGTCTGG
413540R;TCTCCGCCTAGTCTGTCCAA
396596R;TGTATGTCTCCGGCATGTCTG
175082R;AACACAAATACTAGCACTTCCTTT
93422R;GACTTGACTGGGTGCCATACA
341892R;TCTATCTCGCCGGGTACAGT
338450R;CTGGCCCGGCTGCTGTTT
240883R;CACGGTGTGTAGGAGGTGAT
68301R;CCCCGGGTGTGACAAAAACA
12762R;CCATTCGCTCTGCTGTGTGG
34291R;GTTCCTAGCACACCAGCCTT
141260R;GCCTTTAGTCTGGGTCCCAC
355585R;GCTGTCTAGTCTGAGAGCGG
419014R;GACTGCAGCACGATTCTCCT
68079R;GGAAATCCTGGTGCTTCCCA
353453R;AACTGGGCCGGTGAGTAAAC
172755R;TGGCTGCTCTGTGGATAAGC
117597R;GTTCCGGCCCAGCATACAG
20828R;CCGAAGAGCCTGCCTGATAG
221200R;GACCCTCCAAACCTTCGGTC
416138R;TGACATCTTCCGTACCTCCCT
92462R;CAGGCAGGAACCTTAAAAAGAGA
384476R;AGAAAAATCTCCGGTGATCTGAAC
40119R;CATGGAAGTTTGCGCCAAGT
124212R;GCAGGTATGTCTAGGTATACCTT
171913R;GGGGTTACAAAGACCTGCCT
17675R;TGCGCTGTGTGTTGAGTAGT
77946R;GGAACATCTAGTTTCATAACCACTG
347198R;GATGGTCAATCACAAGCTATGGC
51639R;GCCCTCTCCTCTGAAATGGC
260574R;TAGTGAAATCTGGCTCCGGC
70643R;TGCCATACAATTGCTGTGCC
101881R;TGAGCTCCTGGATGGATGGA
12877R;GCACTGGTGTCACGATTGTT
314079R;AGCAGCCACACACATGATCA
142787R;CCTGGGCACTGGACAAATTG
63124R;TACCTTGCGAACACCCCATC
35760R;CGGGGAGCGTAGACTTGG
44615R;GAGAGTGGGAATTGCCACCA
32890R;CACTTATGTTGCCACGCTGG
407099R;CGCCTACCCCCATCCAAAAA
222609R;AGCATTCGACACAACACCCT
76855R;ATACCCAGGTGGCAGAGGT
1202R;ATGTTGTACCCGGGGGAAC
217673R;AGCATGGCTTCCTCTGTTCC
55685R;TTTCTCTCGGCAACAGGGTC
356056R;GTTCCTTCCTGGTAACCTCCG
50837R;AGCAGAGCGGCATTCTACAG
29602R;GCAGCTGTACTACTGGACATGT
34185R;AACCCTTGCCCAGAAACTGT
87020R;ACAGCAACTCCAACTGGTCG
323970R;CCCTGCCAGCTACAGCATAA
172444R;GTGCTGCAGGGCTGTACTAT
33957R;GGAGTCCTCTGCCCCCTAAA
245975R;GGCTCACAGACACGTGGAAT
56086R;TGGGTTTCCTGGTTGTGATGT
168085R;GCAGCAAGTGACGCTTTACA
339387R;GGATAACCATTCCATAGGTGGCA
364672R;GGCACCTAAAACAGAAGGCTC
13658R;GTCTCACAGAGGGGCTTCAC
451505R;CAACATGCCCCGGCCATG
43416R;AGAGACGTGTTTGGCAGCAT
362044R;GCCCAGGTAAGCATATCCAGT
303704R;GACAGGGGAGTATGCAACAGA
99689R;GGCCAAGCATCCTAGAGTGG
148683R;AAATGCACCCAGTGTTCCCA
138480R;GGCAAATTGCTCCCAAGTGG
219992R;CTCCTCCCTGGCCAGTCT
409232R;TTTCGCTCCCTGTATTCGCA
129078R;CTGCCATGCCTATAGGTGCA
125717R;GGTATGCAGTATCCCCTCCC
100084R;TTCTTGTCAGCCACCCGTAC
78301R;TCACTAGTGAAGGGGGGGTA
70988R;ATAAATACAACCCCCGCCCC
205997R;GCTTTCTAACCCTGGCAACC
331742R;CCCACTATGCAAGCACGCTA
285096R;CGGGATGCCTAGCTGAGAAG
142149R;GAACTCCCAGATCCCCCCTA
44930R;CCCCTGCCGGCAGTGTTG
33592R;GATGGTGCTCCTGGGAAC
82745R;AGCCCAGTTTCAAATTTGGC
389468R;TGGCAAAACTAGCATTGGGC
254241R;AGGCAGTAAGTCTTTGGAACCA
208366R;ACTAGTAGCGTCAAAGCATCCA
16227R;GATCGTTACCAGGCCACCAA
13181R;GGGCATCAATAGGAATCTAATATCT
288020R;GGCAAAACACTACGCTTGCA
1428R;AGTGGTGTGGCCTTTGCTC
27619R;GGGTCAATAATGGGTGGGCA
50198R;GGGTCTCCCATGGCATTTACT
324128R;GTTGCCGGACATAGAGGC
409502R;CTCAGTTTTCCAGGCACAGAAC
41673R;ACCACACCTTTCTTGCAGGT
187653R;GGACCGCCAGCTGTACATTT
359057R;GGGGGGGAAAGTTCTAGCAG
411033R;CACACCTAGCAATGTGTGTGT
60328R;CCATGCCGGGATCCTTTCA
15072R;CCCAACCAAAGGGCCAGATT
51596R;TCCTGGTGTCCGGAGTTTTT
145840R;GGAAGTAAGGGTGGCTGTCG
45646R;TGCTAGTTTCAGATGTAGCCAAA
347983R;GTGGCTCTAGCGAGAACGTC
219156R;GTGATTGGCCAGTGCAAATGA
92486R;TCCCCGGCAGAACGTTAC
444797R;TAGCCATGGGAGCCAGTACT
259223R;TGGTTTGTCACTGTACTGCCT
183897R;GGAAAAACCCAGGTACTACCAC
323150R;ACTCACTACACTCAAGTACAGCA
332149R;AAGCTCACCTGGTGCAAGAG
388276R;CCGGGGGAACTCATGGTG
367783R;GGGCAAGGGGGAATGACTTT
41887R;CTAGGGTGTTGGGTGCCC
120611R;ATCCTTCACAAATTCATCCTTCA
301286R;CAAAGCCGGGTTCCTAGCC
315103R;TGGATCTTCCAGGCTGGA
10358R;AGGGTGCCCTGGGGTGCC
421358R;CACTAGTGTCAGGAGACTCATGT
In [186]:
print(min([item.primers["rightprimer_tm"] for item in exp50_nooverlap]))
print(max([item.primers["rightprimer_tm"] for item in exp50_nooverlap]))
print(min([item.primers["leftprimer_tm"] for item in exp50_nooverlap]))
print(max([item.primers["leftprimer_tm"] for item in exp50_nooverlap]))
56.0896547539
66.8857041333
56.0715342465
65.4418333702
In [187]:
import pylab
%pylab inline
hist([abs(float(item.primers["rightprimer_tm"])-float(item.primers["leftprimer_tm"])) for item in exp50_nooverlap])
Populating the interactive namespace from numpy and matplotlib
WARNING: pylab import has clobbered these variables: ['copy', 'pylab']
`%matplotlib` prevents importing * from pylab and numpy
Out[187]:
(array([ 336., 43., 9., 10., 10., 8., 5., 4., 2., 5.]),
array([ 0. , 0.49826619, 0.99653238, 1.49479858, 1.99306477,
2.49133096, 2.98959715, 3.48786334, 3.98612953, 4.48439573,
4.98266192]),
<a list of 10 Patch objects>)
In [197]:
len(set([(str(item.permissible_region)) for item in exp50_nooverlap]))
Out[197]:
432
In [203]:
zip([item.primers["product_length"] for item in exp50bylen[0:216]], [item.primers["product_length"] for item in exp50bylen[216:]])
Out[203]:
[(6133, 2261),
(6105, 2252),
(5895, 2247),
(5698, 2246),
(5686, 2246),
(5643, 2240),
(5146, 2238),
(5045, 2236),
(4941, 2235),
(4838, 2231),
(4766, 2226),
(4700, 2223),
(4693, 2222),
(4666, 2219),
(4438, 2219),
(4306, 2218),
(4269, 2216),
(4262, 2208),
(4232, 2205),
(4229, 2200),
(4097, 2191),
(4065, 2177),
(3996, 2172),
(3978, 2166),
(3930, 2160),
(3921, 2156),
(3905, 2147),
(3900, 2145),
(3881, 2143),
(3822, 2142),
(3812, 2139),
(3803, 2136),
(3779, 2133),
(3720, 2127),
(3710, 2126),
(3693, 2121),
(3667, 2114),
(3666, 2114),
(3658, 2107),
(3645, 2107),
(3626, 2106),
(3622, 2073),
(3568, 2069),
(3563, 2066),
(3515, 2053),
(3511, 2046),
(3447, 2042),
(3445, 2038),
(3410, 2037),
(3394, 2034),
(3392, 2030),
(3389, 2027),
(3387, 2024),
(3387, 2023),
(3377, 2022),
(3374, 2020),
(3372, 2019),
(3363, 2013),
(3359, 2013),
(3357, 2007),
(3351, 1985),
(3337, 1984),
(3319, 1983),
(3294, 1981),
(3286, 1977),
(3265, 1974),
(3263, 1971),
(3257, 1958),
(3252, 1956),
(3252, 1955),
(3239, 1950),
(3230, 1939),
(3227, 1935),
(3223, 1928),
(3222, 1919),
(3221, 1915),
(3213, 1914),
(3212, 1905),
(3209, 1904),
(3202, 1900),
(3188, 1891),
(3169, 1888),
(3167, 1877),
(3163, 1869),
(3160, 1861),
(3131, 1860),
(3130, 1851),
(3109, 1846),
(3106, 1845),
(3089, 1831),
(3087, 1822),
(3081, 1819),
(3077, 1819),
(3064, 1815),
(3049, 1811),
(3047, 1806),
(3044, 1797),
(3027, 1782),
(3010, 1774),
(3002, 1763),
(3001, 1748),
(2986, 1746),
(2981, 1742),
(2980, 1726),
(2961, 1713),
(2954, 1700),
(2945, 1699),
(2940, 1695),
(2936, 1695),
(2936, 1687),
(2935, 1685),
(2933, 1681),
(2909, 1680),
(2907, 1679),
(2903, 1667),
(2891, 1666),
(2884, 1661),
(2880, 1661),
(2871, 1650),
(2862, 1647),
(2862, 1644),
(2852, 1641),
(2849, 1636),
(2831, 1631),
(2828, 1621),
(2828, 1615),
(2816, 1610),
(2812, 1595),
(2806, 1587),
(2806, 1585),
(2802, 1585),
(2799, 1578),
(2766, 1577),
(2760, 1575),
(2758, 1558),
(2746, 1549),
(2744, 1549),
(2739, 1547),
(2725, 1544),
(2719, 1535),
(2712, 1525),
(2697, 1522),
(2686, 1508),
(2682, 1506),
(2680, 1491),
(2664, 1490),
(2659, 1483),
(2657, 1474),
(2653, 1471),
(2652, 1468),
(2648, 1465),
(2645, 1463),
(2643, 1461),
(2642, 1457),
(2634, 1455),
(2632, 1449),
(2611, 1446),
(2599, 1441),
(2592, 1440),
(2589, 1435),
(2577, 1429),
(2572, 1429),
(2562, 1427),
(2560, 1425),
(2557, 1416),
(2549, 1410),
(2547, 1406),
(2542, 1396),
(2530, 1394),
(2530, 1383),
(2529, 1354),
(2522, 1327),
(2508, 1322),
(2501, 1320),
(2482, 1302),
(2472, 1295),
(2471, 1277),
(2464, 1277),
(2460, 1269),
(2455, 1255),
(2452, 1248),
(2450, 1240),
(2439, 1212),
(2438, 1192),
(2433, 1184),
(2425, 1182),
(2423, 1168),
(2416, 1160),
(2414, 1139),
(2407, 1132),
(2405, 1116),
(2395, 1107),
(2382, 1105),
(2378, 1104),
(2377, 1101),
(2370, 1100),
(2367, 1099),
(2367, 1094),
(2359, 1085),
(2356, 1072),
(2353, 1061),
(2352, 1055),
(2351, 1052),
(2350, 1036),
(2343, 992),
(2336, 962),
(2334, 948),
(2333, 918),
(2325, 865),
(2307, 842),
(2287, 834),
(2287, 817),
(2284, 798),
(2279, 695),
(2278, 647),
(2262, 566)]
In [204]:
zip([item.primers["product_length"] for item in exp50bylen[0:144]], [item.primers["product_length"] for item in exp50bylen[144:288]], [item.primers["product_length"] for item in exp50bylen[288:]])
Out[204]:
[(6133, 2680, 1935),
(6105, 2664, 1928),
(5895, 2659, 1919),
(5698, 2657, 1915),
(5686, 2653, 1914),
(5643, 2652, 1905),
(5146, 2648, 1904),
(5045, 2645, 1900),
(4941, 2643, 1891),
(4838, 2642, 1888),
(4766, 2634, 1877),
(4700, 2632, 1869),
(4693, 2611, 1861),
(4666, 2599, 1860),
(4438, 2592, 1851),
(4306, 2589, 1846),
(4269, 2577, 1845),
(4262, 2572, 1831),
(4232, 2562, 1822),
(4229, 2560, 1819),
(4097, 2557, 1819),
(4065, 2549, 1815),
(3996, 2547, 1811),
(3978, 2542, 1806),
(3930, 2530, 1797),
(3921, 2530, 1782),
(3905, 2529, 1774),
(3900, 2522, 1763),
(3881, 2508, 1748),
(3822, 2501, 1746),
(3812, 2482, 1742),
(3803, 2472, 1726),
(3779, 2471, 1713),
(3720, 2464, 1700),
(3710, 2460, 1699),
(3693, 2455, 1695),
(3667, 2452, 1695),
(3666, 2450, 1687),
(3658, 2439, 1685),
(3645, 2438, 1681),
(3626, 2433, 1680),
(3622, 2425, 1679),
(3568, 2423, 1667),
(3563, 2416, 1666),
(3515, 2414, 1661),
(3511, 2407, 1661),
(3447, 2405, 1650),
(3445, 2395, 1647),
(3410, 2382, 1644),
(3394, 2378, 1641),
(3392, 2377, 1636),
(3389, 2370, 1631),
(3387, 2367, 1621),
(3387, 2367, 1615),
(3377, 2359, 1610),
(3374, 2356, 1595),
(3372, 2353, 1587),
(3363, 2352, 1585),
(3359, 2351, 1585),
(3357, 2350, 1578),
(3351, 2343, 1577),
(3337, 2336, 1575),
(3319, 2334, 1558),
(3294, 2333, 1549),
(3286, 2325, 1549),
(3265, 2307, 1547),
(3263, 2287, 1544),
(3257, 2287, 1535),
(3252, 2284, 1525),
(3252, 2279, 1522),
(3239, 2278, 1508),
(3230, 2262, 1506),
(3227, 2261, 1491),
(3223, 2252, 1490),
(3222, 2247, 1483),
(3221, 2246, 1474),
(3213, 2246, 1471),
(3212, 2240, 1468),
(3209, 2238, 1465),
(3202, 2236, 1463),
(3188, 2235, 1461),
(3169, 2231, 1457),
(3167, 2226, 1455),
(3163, 2223, 1449),
(3160, 2222, 1446),
(3131, 2219, 1441),
(3130, 2219, 1440),
(3109, 2218, 1435),
(3106, 2216, 1429),
(3089, 2208, 1429),
(3087, 2205, 1427),
(3081, 2200, 1425),
(3077, 2191, 1416),
(3064, 2177, 1410),
(3049, 2172, 1406),
(3047, 2166, 1396),
(3044, 2160, 1394),
(3027, 2156, 1383),
(3010, 2147, 1354),
(3002, 2145, 1327),
(3001, 2143, 1322),
(2986, 2142, 1320),
(2981, 2139, 1302),
(2980, 2136, 1295),
(2961, 2133, 1277),
(2954, 2127, 1277),
(2945, 2126, 1269),
(2940, 2121, 1255),
(2936, 2114, 1248),
(2936, 2114, 1240),
(2935, 2107, 1212),
(2933, 2107, 1192),
(2909, 2106, 1184),
(2907, 2073, 1182),
(2903, 2069, 1168),
(2891, 2066, 1160),
(2884, 2053, 1139),
(2880, 2046, 1132),
(2871, 2042, 1116),
(2862, 2038, 1107),
(2862, 2037, 1105),
(2852, 2034, 1104),
(2849, 2030, 1101),
(2831, 2027, 1100),
(2828, 2024, 1099),
(2828, 2023, 1094),
(2816, 2022, 1085),
(2812, 2020, 1072),
(2806, 2019, 1061),
(2806, 2013, 1055),
(2802, 2013, 1052),
(2799, 2007, 1036),
(2766, 1985, 992),
(2760, 1984, 962),
(2758, 1983, 948),
(2746, 1981, 918),
(2744, 1977, 865),
(2739, 1974, 842),
(2725, 1971, 834),
(2719, 1958, 817),
(2712, 1956, 798),
(2697, 1955, 695),
(2686, 1950, 647),
(2682, 1939, 566)]
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Content source: eatingcrispr/VirtualEating
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